Q03660 (TR130_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trafficking protein particle complex II-specific subunit 130
Short name=TRAPP II-specific subunit 130

Alternative name(s):
Transport protein particle 130 kDa subunit

Gene names

Name:	TRS130
Ordered Locus Names:	YMR218C
ORF Names:	YM8261.12C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1102 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specific subunit of the TRAPP II complex, a highly conserved vesicle tethering complex that functions in the late Golgi as a guanine nucleotide exchange factor (GEF) for the Golgi YPT1 GTPase. TRS130 plays a role in the YPT GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits TRS65 and TRS120. Ref.4 Ref.5 Ref.7 Ref.8
Subunit structure	Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS120 and TRS130. Ref.3 Ref.5 Ref.8
Subcellular location	Golgi apparatus Probable Ref.5.
Miscellaneous	Present with 432 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TMEM1 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Golgi apparatus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CVT pathway Inferred from mutant phenotype PubMed 23078654. Source: SGD early endosome to Golgi transport Inferred from mutant phenotype PubMed 16314430. Source: SGD intra-Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.5PubMed 16314430. Source: SGD macroautophagy Inferred from mutant phenotype PubMed 23078654. Source: SGD regulation of Rab GTPase activity Inferred from mutant phenotype PubMed 17041589. Source: SGD
Cellular_component	TRAPP complex Inferred from direct assay Ref.4. Source: SGD early endosome Inferred from direct assay PubMed 16314430. Source: SGD trans-Golgi network Inferred from direct assay PubMed 16314430. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1102	1102	Trafficking protein particle complex II-specific subunit 130		PRO_0000193512

Sequences

	Sequence		Length	Mass (Da)
	Q03660 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 110EA9D95620E727	FASTA	1,102	128,131
	10 20 30 40 50 60 MDKEIYCGSV PVSYFDPFDL FESLRPEFQQ ILPLDNIHWK AFDGTVRTVN RLPIELIPEG 70 80 90 100 110 120 RGEADKSNDE QPFIRFLIVN CISIDQYRAK VRPLVRQWLP NLESVSSSTG EKMIYKPIIL 130 140 150 160 170 180 LYANSEVVDS NLFKSVSLME KFGKDFPHVQ TLEVRSVYRS PKERQEFWNQ FSQKIKASVL 190 200 210 220 230 240 SIFQKRLTHL QHSLANLQKG NNFEEQLLTR EKLYELYVVF NILEDASLEL QKIKKEILRR 250 260 270 280 290 300 NMNMPDGKLQ VPFESSSKSD ESLGSIIIEG TLDKFQLHKY FFIRRLRLLK LEDQTLTAFV 310 320 330 340 350 360 GAFQLIKNFI ESISIEYRKS VRLLEFKHYF ITSMLSYFEF ENVSNPLLCE IKAELLMLKR 370 380 390 400 410 420 DNWVQGVMAT SGYRLMDKNY PNSDVKYKFD LLKETFVDET VFQENFLTLT KEILSLFNKC 430 440 450 460 470 480 EGKRQRIVDI LSIEIGLLYY QGKKYEEAVS LFLSCYEYYT QTNWNSIGLK ILQVFIDSLS 490 500 510 520 530 540 HCPKLDVLQI DGESVSASAV LTNAFLNILK LCKDNDSKEI WWKKFMDLQM KNNIHLMYPL 550 560 570 580 590 600 DGLFEVTLNS KVHLARANVS AIEVNLKSYG FPEDISTKTM RLSLKNMGGD VIVFGASDFL 610 620 630 640 650 660 LKKGENKLIL ECRDIMYGEF SLLSFEIIVE GITFVKEFPE NQDEFIVVPE IYCKESTKVL 670 680 690 700 710 720 VKQAHNLNLG EYALELKSVQ SDALESLQVE VEVQKNIGNM KNLPVSFSMD EIQARKRYNT 730 740 750 760 770 780 PFENVRLEYY LLDQITAFDL IIKTSFTKKN DQGTFGETKK VRIQCYLQLS VSVEDIFKKD 790 800 810 820 830 840 IFFFKFLLNS SVREEPVILY SSELSAPDTR NDYNIRGDYI ATTPALITFD GNESFINCYE 850 860 870 880 890 900 ITANNNFDSK DIFNLKVRYN TLKEQLDCFI TDAVLIEGDV EWFILFEKWK TFWELEILKK 910 920 930 940 950 960 LKYDYDAFKE NRIIRLLKTS IDLNKTKSKI RNLCIEKAVL DKILICLNKV SRGIAVCNTD 970 980 990 1000 1010 1020 MDEYVRNLVP KQLTVPVQLP GFEQFFHVQF EQMETSHDAL HDTIATIGNS LSYTVIVENL 1030 1040 1050 1060 1070 1080 SGQWGQDVID DGGYIFEILS SNEWLIHGQK RCAIKEKRKE FEVHLIPLKK GYLNFPRVEI 1090 1100 TNINGKSCRV DHSNAFESIL IF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion." Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S. EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
[4]	"Identification and characterization of five new subunits of TRAPP." Sacher M., Barrowman J., Schieltz D., Yates J.R. III, Ferro-Novick S. Eur. J. Cell Biol. 79:71-80(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport." Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S. Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex." Liang Y., Morozova N., Tokarev A.A., Mulholland J.W., Segev N. Mol. Biol. Cell 18:2533-2541(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TRS65.
[8]	"Molecular architecture of the TRAPPII complex and implications for vesicle tethering." Yip C.K., Berscheminski J., Walz T. Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49809 Genomic DNA. Translation: CAA89933.1. BK006946 Genomic DNA. Translation: DAA10117.1.
PIR	S55100.
RefSeq	NP_013945.1. NM_001182725.1.
3D structure databases
ProteinModelPortal	Q03660.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6482N.
IntAct	Q03660. 9 interactions.
MINT	MINT-628621.
STRING	4932.YMR218C.
Proteomic databases
PaxDb	Q03660.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR218C; YMR218C; YMR218C.
GeneID	855258.
KEGG	sce:YMR218C.
Organism-specific databases
CYGD	YMR218c.
SGD	S000004831. TRS130.
Phylogenomic databases
eggNOG	NOG307398.
HOGENOM	HOG000142365.
OMA	SKFQVGT.
OrthoDB	EOG42FWRV.
Gene expression databases
Genevestigator	Q03660.
GermOnline	YMR218C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022233. TRAPP_II_complex_TRAPPC10. [Graphical view]
Pfam	PF12584. TRAPPC10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978846.

Entry information

Entry name

TR130_YEAST

Accession

Primary (citable) accession number: Q03660
Secondary accession number(s): D6W043

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program