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Q03660

- TR130_YEAST

UniProt

Q03660 - TR130_YEAST

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Protein

Trafficking protein particle complex II-specific subunit 130

Gene

TRS130

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specific subunit of the TRAPP II complex, a highly conserved vesicle tethering complex that functions in the late Golgi as a guanine nucleotide exchange factor (GEF) for the Golgi YPT1 GTPase. TRS130 plays a role in the YPT GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits TRS65 and TRS120. Required for both the cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy through its role in ATG8 and ATG9 trafficking.7 Publications

GO - Biological processi

  1. CVT pathway Source: SGD
  2. early endosome to Golgi transport Source: SGD
  3. intra-Golgi vesicle-mediated transport Source: SGD
  4. macroautophagy Source: SGD
  5. positive regulation of Rab GTPase activity Source: GOC
  6. regulation of Rab GTPase activity Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking protein particle complex II-specific subunit 130
Short name:
TRAPP II-specific subunit 130
Alternative name(s):
Transport protein particle 130 kDa subunit
Gene namesi
Name:TRS130
Ordered Locus Names:YMR218C
ORF Names:YM8261.12C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR218c.
SGDiS000004831. TRS130.

Subcellular locationi

Golgi apparatus 1 Publication

GO - Cellular componenti

  1. early endosome Source: SGD
  2. trans-Golgi network Source: SGD
  3. TRAPPII protein complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11021102Trafficking protein particle complex II-specific subunit 130PRO_0000193512Add
BLAST

Proteomic databases

MaxQBiQ03660.
PaxDbiQ03660.

Expressioni

Gene expression databases

GenevestigatoriQ03660.

Interactioni

Subunit structurei

Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS120 and TRS130. Interacts with YPT31 and YPT32.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TCA17P326137EBI-19461,EBI-22370
TRS20P383343EBI-19461,EBI-19468

Protein-protein interaction databases

BioGridi35396. 39 interactions.
DIPiDIP-6482N.
IntActiQ03660. 9 interactions.
MINTiMINT-628621.
STRINGi4932.YMR218C.

Structurei

3D structure databases

ProteinModelPortaliQ03660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TMEM1 family.Curated

Phylogenomic databases

eggNOGiNOG307398.
HOGENOMiHOG000142365.
InParanoidiQ03660.
OMAiSYFDPFD.
OrthoDBiEOG7966RM.

Family and domain databases

InterProiIPR022233. TRAPP_II_complex_TRAPPC10.
[Graphical view]
PfamiPF12584. TRAPPC10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03660-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKEIYCGSV PVSYFDPFDL FESLRPEFQQ ILPLDNIHWK AFDGTVRTVN
60 70 80 90 100
RLPIELIPEG RGEADKSNDE QPFIRFLIVN CISIDQYRAK VRPLVRQWLP
110 120 130 140 150
NLESVSSSTG EKMIYKPIIL LYANSEVVDS NLFKSVSLME KFGKDFPHVQ
160 170 180 190 200
TLEVRSVYRS PKERQEFWNQ FSQKIKASVL SIFQKRLTHL QHSLANLQKG
210 220 230 240 250
NNFEEQLLTR EKLYELYVVF NILEDASLEL QKIKKEILRR NMNMPDGKLQ
260 270 280 290 300
VPFESSSKSD ESLGSIIIEG TLDKFQLHKY FFIRRLRLLK LEDQTLTAFV
310 320 330 340 350
GAFQLIKNFI ESISIEYRKS VRLLEFKHYF ITSMLSYFEF ENVSNPLLCE
360 370 380 390 400
IKAELLMLKR DNWVQGVMAT SGYRLMDKNY PNSDVKYKFD LLKETFVDET
410 420 430 440 450
VFQENFLTLT KEILSLFNKC EGKRQRIVDI LSIEIGLLYY QGKKYEEAVS
460 470 480 490 500
LFLSCYEYYT QTNWNSIGLK ILQVFIDSLS HCPKLDVLQI DGESVSASAV
510 520 530 540 550
LTNAFLNILK LCKDNDSKEI WWKKFMDLQM KNNIHLMYPL DGLFEVTLNS
560 570 580 590 600
KVHLARANVS AIEVNLKSYG FPEDISTKTM RLSLKNMGGD VIVFGASDFL
610 620 630 640 650
LKKGENKLIL ECRDIMYGEF SLLSFEIIVE GITFVKEFPE NQDEFIVVPE
660 670 680 690 700
IYCKESTKVL VKQAHNLNLG EYALELKSVQ SDALESLQVE VEVQKNIGNM
710 720 730 740 750
KNLPVSFSMD EIQARKRYNT PFENVRLEYY LLDQITAFDL IIKTSFTKKN
760 770 780 790 800
DQGTFGETKK VRIQCYLQLS VSVEDIFKKD IFFFKFLLNS SVREEPVILY
810 820 830 840 850
SSELSAPDTR NDYNIRGDYI ATTPALITFD GNESFINCYE ITANNNFDSK
860 870 880 890 900
DIFNLKVRYN TLKEQLDCFI TDAVLIEGDV EWFILFEKWK TFWELEILKK
910 920 930 940 950
LKYDYDAFKE NRIIRLLKTS IDLNKTKSKI RNLCIEKAVL DKILICLNKV
960 970 980 990 1000
SRGIAVCNTD MDEYVRNLVP KQLTVPVQLP GFEQFFHVQF EQMETSHDAL
1010 1020 1030 1040 1050
HDTIATIGNS LSYTVIVENL SGQWGQDVID DGGYIFEILS SNEWLIHGQK
1060 1070 1080 1090 1100
RCAIKEKRKE FEVHLIPLKK GYLNFPRVEI TNINGKSCRV DHSNAFESIL

IF
Length:1,102
Mass (Da):128,131
Last modified:November 1, 1997 - v1
Checksum:i110EA9D95620E727
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49809 Genomic DNA. Translation: CAA89933.1.
BK006946 Genomic DNA. Translation: DAA10117.1.
PIRiS55100.
RefSeqiNP_013945.1. NM_001182725.1.

Genome annotation databases

EnsemblFungiiYMR218C; YMR218C; YMR218C.
GeneIDi855258.
KEGGisce:YMR218C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49809 Genomic DNA. Translation: CAA89933.1 .
BK006946 Genomic DNA. Translation: DAA10117.1 .
PIRi S55100.
RefSeqi NP_013945.1. NM_001182725.1.

3D structure databases

ProteinModelPortali Q03660.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35396. 39 interactions.
DIPi DIP-6482N.
IntActi Q03660. 9 interactions.
MINTi MINT-628621.
STRINGi 4932.YMR218C.

Proteomic databases

MaxQBi Q03660.
PaxDbi Q03660.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR218C ; YMR218C ; YMR218C .
GeneIDi 855258.
KEGGi sce:YMR218C.

Organism-specific databases

CYGDi YMR218c.
SGDi S000004831. TRS130.

Phylogenomic databases

eggNOGi NOG307398.
HOGENOMi HOG000142365.
InParanoidi Q03660.
OMAi SYFDPFD.
OrthoDBi EOG7966RM.

Enzyme and pathway databases

BioCyci YEAST:G3O-32900-MONOMER.

Miscellaneous databases

NextBioi 978846.
PROi Q03660.

Gene expression databases

Genevestigatori Q03660.

Family and domain databases

InterProi IPR022233. TRAPP_II_complex_TRAPPC10.
[Graphical view ]
Pfami PF12584. TRAPPC10. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
    Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
    EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
  4. "Identification and characterization of five new subunits of TRAPP."
    Sacher M., Barrowman J., Schieltz D., Yates J.R. III, Ferro-Novick S.
    Eur. J. Cell Biol. 79:71-80(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
    Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
    Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
  6. "Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
    Yamamoto K., Jigami Y.
    Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH YPT31 AND YPT32.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Mutants in trs120 disrupt traffic from the early endosome to the late Golgi."
    Cai H., Zhang Y., Pypaert M., Walker L., Ferro-Novick S.
    J. Cell Biol. 171:823-833(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex."
    Liang Y., Morozova N., Tokarev A.A., Mulholland J.W., Segev N.
    Mol. Biol. Cell 18:2533-2541(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRS65.
  10. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
    Yip C.K., Berscheminski J., Walz T.
    Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
  11. "Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
    Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
    Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTR130_YEAST
AccessioniPrimary (citable) accession number: Q03660
Secondary accession number(s): D6W043
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3