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Q03660 (TR130_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trafficking protein particle complex II-specific subunit 130

Short name=TRAPP II-specific subunit 130
Alternative name(s):
Transport protein particle 130 kDa subunit
Gene names
Name:TRS130
Ordered Locus Names:YMR218C
ORF Names:YM8261.12C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1102 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specific subunit of the TRAPP II complex, a highly conserved vesicle tethering complex that functions in the late Golgi as a guanine nucleotide exchange factor (GEF) for the Golgi YPT1 GTPase. TRS130 plays a role in the YPT GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits TRS65 and TRS120. Required for both the cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy through its role in ATG8 and ATG9 trafficking. Ref.4 Ref.5 Ref.6 Ref.8 Ref.9 Ref.10 Ref.11

Subunit structure

Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS120 and TRS130. Interacts with YPT31 and YPT32. Ref.3 Ref.5 Ref.6 Ref.9 Ref.10

Subcellular location

Golgi apparatus Probable Ref.5.

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TMEM1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TCA17P326137EBI-19461,EBI-22370
TRS20P383343EBI-19461,EBI-19468

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11021102Trafficking protein particle complex II-specific subunit 130
PRO_0000193512

Sequences

Sequence LengthMass (Da)Tools
Q03660 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 110EA9D95620E727

FASTA1,102128,131
        10         20         30         40         50         60 
MDKEIYCGSV PVSYFDPFDL FESLRPEFQQ ILPLDNIHWK AFDGTVRTVN RLPIELIPEG 

        70         80         90        100        110        120 
RGEADKSNDE QPFIRFLIVN CISIDQYRAK VRPLVRQWLP NLESVSSSTG EKMIYKPIIL 

       130        140        150        160        170        180 
LYANSEVVDS NLFKSVSLME KFGKDFPHVQ TLEVRSVYRS PKERQEFWNQ FSQKIKASVL 

       190        200        210        220        230        240 
SIFQKRLTHL QHSLANLQKG NNFEEQLLTR EKLYELYVVF NILEDASLEL QKIKKEILRR 

       250        260        270        280        290        300 
NMNMPDGKLQ VPFESSSKSD ESLGSIIIEG TLDKFQLHKY FFIRRLRLLK LEDQTLTAFV 

       310        320        330        340        350        360 
GAFQLIKNFI ESISIEYRKS VRLLEFKHYF ITSMLSYFEF ENVSNPLLCE IKAELLMLKR 

       370        380        390        400        410        420 
DNWVQGVMAT SGYRLMDKNY PNSDVKYKFD LLKETFVDET VFQENFLTLT KEILSLFNKC 

       430        440        450        460        470        480 
EGKRQRIVDI LSIEIGLLYY QGKKYEEAVS LFLSCYEYYT QTNWNSIGLK ILQVFIDSLS 

       490        500        510        520        530        540 
HCPKLDVLQI DGESVSASAV LTNAFLNILK LCKDNDSKEI WWKKFMDLQM KNNIHLMYPL 

       550        560        570        580        590        600 
DGLFEVTLNS KVHLARANVS AIEVNLKSYG FPEDISTKTM RLSLKNMGGD VIVFGASDFL 

       610        620        630        640        650        660 
LKKGENKLIL ECRDIMYGEF SLLSFEIIVE GITFVKEFPE NQDEFIVVPE IYCKESTKVL 

       670        680        690        700        710        720 
VKQAHNLNLG EYALELKSVQ SDALESLQVE VEVQKNIGNM KNLPVSFSMD EIQARKRYNT 

       730        740        750        760        770        780 
PFENVRLEYY LLDQITAFDL IIKTSFTKKN DQGTFGETKK VRIQCYLQLS VSVEDIFKKD 

       790        800        810        820        830        840 
IFFFKFLLNS SVREEPVILY SSELSAPDTR NDYNIRGDYI ATTPALITFD GNESFINCYE 

       850        860        870        880        890        900 
ITANNNFDSK DIFNLKVRYN TLKEQLDCFI TDAVLIEGDV EWFILFEKWK TFWELEILKK 

       910        920        930        940        950        960 
LKYDYDAFKE NRIIRLLKTS IDLNKTKSKI RNLCIEKAVL DKILICLNKV SRGIAVCNTD 

       970        980        990       1000       1010       1020 
MDEYVRNLVP KQLTVPVQLP GFEQFFHVQF EQMETSHDAL HDTIATIGNS LSYTVIVENL 

      1030       1040       1050       1060       1070       1080 
SGQWGQDVID DGGYIFEILS SNEWLIHGQK RCAIKEKRKE FEVHLIPLKK GYLNFPRVEI 

      1090       1100 
TNINGKSCRV DHSNAFESIL IF 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
[4]"Identification and characterization of five new subunits of TRAPP."
Sacher M., Barrowman J., Schieltz D., Yates J.R. III, Ferro-Novick S.
Eur. J. Cell Biol. 79:71-80(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
[6]"Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
Yamamoto K., Jigami Y.
Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH YPT31 AND YPT32.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Mutants in trs120 disrupt traffic from the early endosome to the late Golgi."
Cai H., Zhang Y., Pypaert M., Walker L., Ferro-Novick S.
J. Cell Biol. 171:823-833(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex."
Liang Y., Morozova N., Tokarev A.A., Mulholland J.W., Segev N.
Mol. Biol. Cell 18:2533-2541(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TRS65.
[10]"Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
Yip C.K., Berscheminski J., Walz T.
Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[11]"Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49809 Genomic DNA. Translation: CAA89933.1.
BK006946 Genomic DNA. Translation: DAA10117.1.
PIRS55100.
RefSeqNP_013945.1. NM_001182725.1.

3D structure databases

ProteinModelPortalQ03660.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35396. 39 interactions.
DIPDIP-6482N.
IntActQ03660. 9 interactions.
MINTMINT-628621.
STRING4932.YMR218C.

Proteomic databases

MaxQBQ03660.
PaxDbQ03660.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR218C; YMR218C; YMR218C.
GeneID855258.
KEGGsce:YMR218C.

Organism-specific databases

CYGDYMR218c.
SGDS000004831. TRS130.

Phylogenomic databases

eggNOGNOG307398.
HOGENOMHOG000142365.
OMASYFDPFD.
OrthoDBEOG7966RM.

Enzyme and pathway databases

BioCycYEAST:G3O-32900-MONOMER.

Gene expression databases

GenevestigatorQ03660.

Family and domain databases

InterProIPR022233. TRAPP_II_complex_TRAPPC10.
[Graphical view]
PfamPF12584. TRAPPC10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978846.
PROQ03660.

Entry information

Entry nameTR130_YEAST
AccessionPrimary (citable) accession number: Q03660
Secondary accession number(s): D6W043
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families