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Q03660

- TR130_YEAST

UniProt

Q03660 - TR130_YEAST

Protein

Trafficking protein particle complex II-specific subunit 130

Gene

TRS130

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Specific subunit of the TRAPP II complex, a highly conserved vesicle tethering complex that functions in the late Golgi as a guanine nucleotide exchange factor (GEF) for the Golgi YPT1 GTPase. TRS130 plays a role in the YPT GEF activity of TRAPP II in concert with the two other TRAPP II-specific subunits TRS65 and TRS120. Required for both the cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy through its role in ATG8 and ATG9 trafficking.7 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. CVT pathway Source: SGD
    2. early endosome to Golgi transport Source: SGD
    3. intra-Golgi vesicle-mediated transport Source: SGD
    4. macroautophagy Source: SGD
    5. positive regulation of Rab GTPase activity Source: GOC
    6. regulation of Rab GTPase activity Source: SGD

    Keywords - Biological processi

    Autophagy, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32900-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trafficking protein particle complex II-specific subunit 130
    Short name:
    TRAPP II-specific subunit 130
    Alternative name(s):
    Transport protein particle 130 kDa subunit
    Gene namesi
    Name:TRS130
    Ordered Locus Names:YMR218C
    ORF Names:YM8261.12C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR218c.
    SGDiS000004831. TRS130.

    Subcellular locationi

    Golgi apparatus 1 Publication

    GO - Cellular componenti

    1. early endosome Source: SGD
    2. trans-Golgi network Source: SGD
    3. TRAPPII protein complex Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11021102Trafficking protein particle complex II-specific subunit 130PRO_0000193512Add
    BLAST

    Proteomic databases

    MaxQBiQ03660.
    PaxDbiQ03660.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03660.

    Interactioni

    Subunit structurei

    Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS120 and TRS130. Interacts with YPT31 and YPT32.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TCA17P326137EBI-19461,EBI-22370
    TRS20P383343EBI-19461,EBI-19468

    Protein-protein interaction databases

    BioGridi35396. 39 interactions.
    DIPiDIP-6482N.
    IntActiQ03660. 9 interactions.
    MINTiMINT-628621.
    STRINGi4932.YMR218C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03660.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TMEM1 family.Curated

    Phylogenomic databases

    eggNOGiNOG307398.
    HOGENOMiHOG000142365.
    OMAiSYFDPFD.
    OrthoDBiEOG7966RM.

    Family and domain databases

    InterProiIPR022233. TRAPP_II_complex_TRAPPC10.
    [Graphical view]
    PfamiPF12584. TRAPPC10. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03660-1 [UniParc]FASTAAdd to Basket

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    MDKEIYCGSV PVSYFDPFDL FESLRPEFQQ ILPLDNIHWK AFDGTVRTVN     50
    RLPIELIPEG RGEADKSNDE QPFIRFLIVN CISIDQYRAK VRPLVRQWLP 100
    NLESVSSSTG EKMIYKPIIL LYANSEVVDS NLFKSVSLME KFGKDFPHVQ 150
    TLEVRSVYRS PKERQEFWNQ FSQKIKASVL SIFQKRLTHL QHSLANLQKG 200
    NNFEEQLLTR EKLYELYVVF NILEDASLEL QKIKKEILRR NMNMPDGKLQ 250
    VPFESSSKSD ESLGSIIIEG TLDKFQLHKY FFIRRLRLLK LEDQTLTAFV 300
    GAFQLIKNFI ESISIEYRKS VRLLEFKHYF ITSMLSYFEF ENVSNPLLCE 350
    IKAELLMLKR DNWVQGVMAT SGYRLMDKNY PNSDVKYKFD LLKETFVDET 400
    VFQENFLTLT KEILSLFNKC EGKRQRIVDI LSIEIGLLYY QGKKYEEAVS 450
    LFLSCYEYYT QTNWNSIGLK ILQVFIDSLS HCPKLDVLQI DGESVSASAV 500
    LTNAFLNILK LCKDNDSKEI WWKKFMDLQM KNNIHLMYPL DGLFEVTLNS 550
    KVHLARANVS AIEVNLKSYG FPEDISTKTM RLSLKNMGGD VIVFGASDFL 600
    LKKGENKLIL ECRDIMYGEF SLLSFEIIVE GITFVKEFPE NQDEFIVVPE 650
    IYCKESTKVL VKQAHNLNLG EYALELKSVQ SDALESLQVE VEVQKNIGNM 700
    KNLPVSFSMD EIQARKRYNT PFENVRLEYY LLDQITAFDL IIKTSFTKKN 750
    DQGTFGETKK VRIQCYLQLS VSVEDIFKKD IFFFKFLLNS SVREEPVILY 800
    SSELSAPDTR NDYNIRGDYI ATTPALITFD GNESFINCYE ITANNNFDSK 850
    DIFNLKVRYN TLKEQLDCFI TDAVLIEGDV EWFILFEKWK TFWELEILKK 900
    LKYDYDAFKE NRIIRLLKTS IDLNKTKSKI RNLCIEKAVL DKILICLNKV 950
    SRGIAVCNTD MDEYVRNLVP KQLTVPVQLP GFEQFFHVQF EQMETSHDAL 1000
    HDTIATIGNS LSYTVIVENL SGQWGQDVID DGGYIFEILS SNEWLIHGQK 1050
    RCAIKEKRKE FEVHLIPLKK GYLNFPRVEI TNINGKSCRV DHSNAFESIL 1100
    IF 1102
    Length:1,102
    Mass (Da):128,131
    Last modified:November 1, 1997 - v1
    Checksum:i110EA9D95620E727
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49809 Genomic DNA. Translation: CAA89933.1.
    BK006946 Genomic DNA. Translation: DAA10117.1.
    PIRiS55100.
    RefSeqiNP_013945.1. NM_001182725.1.

    Genome annotation databases

    EnsemblFungiiYMR218C; YMR218C; YMR218C.
    GeneIDi855258.
    KEGGisce:YMR218C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49809 Genomic DNA. Translation: CAA89933.1 .
    BK006946 Genomic DNA. Translation: DAA10117.1 .
    PIRi S55100.
    RefSeqi NP_013945.1. NM_001182725.1.

    3D structure databases

    ProteinModelPortali Q03660.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35396. 39 interactions.
    DIPi DIP-6482N.
    IntActi Q03660. 9 interactions.
    MINTi MINT-628621.
    STRINGi 4932.YMR218C.

    Proteomic databases

    MaxQBi Q03660.
    PaxDbi Q03660.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR218C ; YMR218C ; YMR218C .
    GeneIDi 855258.
    KEGGi sce:YMR218C.

    Organism-specific databases

    CYGDi YMR218c.
    SGDi S000004831. TRS130.

    Phylogenomic databases

    eggNOGi NOG307398.
    HOGENOMi HOG000142365.
    OMAi SYFDPFD.
    OrthoDBi EOG7966RM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32900-MONOMER.

    Miscellaneous databases

    NextBioi 978846.
    PROi Q03660.

    Gene expression databases

    Genevestigatori Q03660.

    Family and domain databases

    InterProi IPR022233. TRAPP_II_complex_TRAPPC10.
    [Graphical view ]
    Pfami PF12584. TRAPPC10. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
      Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
      EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
    4. "Identification and characterization of five new subunits of TRAPP."
      Sacher M., Barrowman J., Schieltz D., Yates J.R. III, Ferro-Novick S.
      Eur. J. Cell Biol. 79:71-80(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
      Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
      Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
    6. "Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
      Yamamoto K., Jigami Y.
      Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH YPT31 AND YPT32.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Mutants in trs120 disrupt traffic from the early endosome to the late Golgi."
      Cai H., Zhang Y., Pypaert M., Walker L., Ferro-Novick S.
      J. Cell Biol. 171:823-833(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex."
      Liang Y., Morozova N., Tokarev A.A., Mulholland J.W., Segev N.
      Mol. Biol. Cell 18:2533-2541(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRS65.
    10. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
      Yip C.K., Berscheminski J., Walz T.
      Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
    11. "Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
      Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
      Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiTR130_YEAST
    AccessioniPrimary (citable) accession number: Q03660
    Secondary accession number(s): D6W043
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 432 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3