Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase SKY1

Gene

SKY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Constitutively active kinase, specifically and sequentially phosphorylates serine/arginine (SR)-type shuttling mRNA binding proteins in their RS dipeptide repeats.1 Publication

Miscellaneous

Present with 2420 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187ATPPROSITE-ProRule annotation1
Active sitei294Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi164 – 172ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • cellular cation homeostasis Source: SGD
  • cellular ion homeostasis Source: SGD
  • intracellular signal transduction Source: GO_Central
  • mRNA splice site selection Source: SGD
  • positive regulation of protein import into nucleus Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of mRNA processing Source: GO_Central
  • response to drug Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32899-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SKY1 (EC:2.7.11.1)
Short name:
SRPK
Gene namesi
Name:SKY1
Ordered Locus Names:YMR216C
ORF Names:YM8261.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR216C
SGDiS000004829 SKY1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866581 – 742Serine/threonine-protein kinase SKY1Add BLAST742

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei383PhosphothreonineCombined sources1
Modified residuei386PhosphothreonineCombined sources1
Modified residuei388PhosphoserineCombined sources1
Modified residuei393PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1
Modified residuei427PhosphoserineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei445PhosphoserineCombined sources1
Modified residuei449PhosphoserineCombined sources1
Modified residuei453PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03656
PaxDbiQ03656
PRIDEiQ03656

PTM databases

iPTMnetiQ03656

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TOR1P351692EBI-9800,EBI-19374

Protein-protein interaction databases

BioGridi35394, 392 interactors
DIPiDIP-2884N
IntActiQ03656, 16 interactors
MINTiQ03656
STRINGi4932.YMR216C

Structurei

Secondary structure

1742
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni154 – 157Combined sources4
Beta strandi158 – 166Combined sources9
Beta strandi168 – 177Combined sources10
Turni178 – 181Combined sources4
Beta strandi182 – 189Combined sources8
Helixi193 – 211Combined sources19
Helixi216 – 222Combined sources7
Beta strandi230 – 236Combined sources7
Beta strandi239 – 246Combined sources8
Helixi253 – 259Combined sources7
Turni260 – 262Combined sources3
Helixi267 – 286Combined sources20
Helixi297 – 299Combined sources3
Beta strandi300 – 304Combined sources5
Turni539 – 542Combined sources4
Beta strandi543 – 548Combined sources6
Helixi551 – 553Combined sources3
Helixi568 – 570Combined sources3
Helixi573 – 577Combined sources5
Helixi584 – 599Combined sources16
Beta strandi610 – 612Combined sources3
Helixi615 – 626Combined sources12
Helixi631 – 636Combined sources6
Helixi640 – 643Combined sources4
Beta strandi650 – 652Combined sources3
Helixi661 – 667Combined sources7
Helixi673 – 683Combined sources11
Helixi684 – 687Combined sources4
Turni691 – 693Combined sources3
Helixi697 – 701Combined sources5
Helixi704 – 706Combined sources3
Turni710 – 714Combined sources5
Helixi726 – 728Combined sources3
Beta strandi732 – 734Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HOWX-ray2.10A138-304[»]
A539-742[»]
1Q8YX-ray2.05A/B138-304[»]
A/B539-742[»]
1Q8ZX-ray2.35A/B138-304[»]
A/B539-742[»]
1Q97X-ray2.30A/B138-304[»]
A/B539-742[»]
1Q99X-ray2.11A/B138-304[»]
A/B539-742[»]
2JD5X-ray2.50A/B138-742[»]
ProteinModelPortaliQ03656
SMRiQ03656
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03656

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini158 – 706Protein kinasePROSITE-ProRule annotationAdd BLAST549

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063566
HOGENOMiHOG000171558
InParanoidiQ03656
KOiK08832
OMAiNHHFTND
OrthoDBiEOG092C3YVW

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 2 hits
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q03656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSINYPGF VTKSAHLADT STDASISCEE ATSSQEAKKN FFQRDYNMMK
60 70 80 90 100
KAPAPTKSKL SLALQTSKSS SSANGTVQED TSSKTEDFST KSIKKKPDSG
110 120 130 140 150
VESHVSIQSD SGPQSDSDLD SDSSISSCDE RNEESLKDYR PGGYHPAFKG
160 170 180 190 200
EPYKDARYIL VRKLGWGHFS TVWLAKDMVN NTHVAMKIVR GDKVYTEAAE
210 220 230 240 250
DEIKLLQRVN DADNTKEDSM GANHILKLLD HFNHKGPNGV HVVMVFEVLG
260 270 280 290 300
ENLLALIKKY EHRGIPLIYV KQISKQLLLG LDYMHRRCGI IHTDIKPENV
310 320 330 340 350
LMEIGDVEGI VQMVEALDKQ KREAKRLQRH VSRSSDITAN DSSDEKWAEC
360 370 380 390 400
QTSMPCGSSS NSKSRSIEKD LSKRCFRRPR RHTIITGSQP LPSPISSSNF
410 420 430 440 450
FEMRAHFCGS SHNSFSSVSG NRNIPSSINN NSINNGIGIK NSNNSFLNSV
460 470 480 490 500
PHSVTRMFIN EDSNDNNNND NSKNKNNNNN NSNNNNNEDI MNTPLHEEQL
510 520 530 540 550
ADSLSTFDIS NISQSSDTNG PYISNTMDSN SNVSTDINSP ENLIQIKIAD
560 570 580 590 600
LGNACWYDEH YTNSIQTREY RSPEVLLGAP WGCGADIWST ACLIFELITG
610 620 630 640 650
DFLFEPDEGH SYTKDDDHIA QIIELLGELP SYLLRNGKYT RTFFNSRGLL
660 670 680 690 700
RNISKLKFWP LEDVLTEKYK FSKDEAKEIS DFLSPMLQLD PRKRADAGGL
710 720 730 740
VNHPWLKDTL GMEEIRVPDR ELYGSGSDIP GWFEEVRDHK RH
Length:742
Mass (Da):83,238
Last modified:November 1, 1997 - v1
Checksum:iC775F10B30C950FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49809 Genomic DNA Translation: CAA89931.1
BK006946 Genomic DNA Translation: DAA10115.1
PIRiS55098
RefSeqiNP_013943.1, NM_001182723.1

Genome annotation databases

EnsemblFungiiYMR216C; YMR216C; YMR216C
GeneIDi855256
KEGGisce:YMR216C

Similar proteinsi

Entry informationi

Entry nameiSKY1_YEAST
AccessioniPrimary (citable) accession number: Q03656
Secondary accession number(s): D6W041
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health