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Protein

Pre-mRNA-splicing factor CEF1

Gene

CEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing and cell cycle control. Required for the binding of the NTC complex (or PRP19-associated complex) components to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. Its absence leads to an arrest of the cell cycle, possibly due to the inefficient splicing of TUB1.4 Publications

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi33 – 56H-T-H motifPROSITE-ProRule annotationAdd BLAST24
DNA bindingi84 – 106H-T-H motifPROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: GO_Central
  • generation of catalytic spliceosome for second transesterification step Source: SGD
  • mRNA splicing, via spliceosome Source: SGD
  • regulation of transcription by RNA polymerase II Source: GO_Central

Keywordsi

Molecular functionDNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-32896-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CEF1
Alternative name(s):
PRP nineteen-associated complex protein 85
PRP19-associated complex protein 85
Gene namesi
Name:CEF1
Synonyms:NTC85
Ordered Locus Names:YMR213W
ORF Names:YM8261.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR213W
SGDiS000004826 CEF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33W → G: No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-52 and G-84. Complete loss of function; when associated with G-52; G-84 and G-102. 1 Publication1
Mutagenesisi52W → G: No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-33 and G-84. Complete loss of function; when associated with G-33; G-84 and G-102. 2 Publications1
Mutagenesisi84W → G: No effect. Slower growth and thermosensitivity; when associated with G-33 or G-52. Complete loss of function; when associated with G-33 and G-52 or G-52 and Y-102. Complete loss of function; when associated with G-33; G-52 and G-102. 2 Publications1
Mutagenesisi102Y → G: No effect. Slower growth and thermosensitivity; when associated with G-52 or G-84. Complete loss of function; when associated with G-33; G-52 and G-84. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001971071 – 590Pre-mRNA-splicing factor CEF1Add BLAST590

Proteomic databases

MaxQBiQ03654
PaxDbiQ03654
PRIDEiQ03654

PTM databases

iPTMnetiQ03654

Interactioni

Subunit structurei

Belongs to the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts with CLF1, ISY1, NTC20, PRP19, PRP46, SYF1 and SYF2.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35391, 304 interactors
DIPiDIP-1113N
IntActiQ03654, 59 interactors
MINTiQ03654
STRINGi4932.YMR213W

Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 28Combined sources14
Helixi33 – 35Combined sources3
Helixi36 – 39Combined sources4
Helixi45 – 54Combined sources10
Helixi67 – 79Combined sources13
Beta strandi80 – 82Combined sources3
Helixi86 – 91Combined sources6
Helixi95 – 106Combined sources12
Helixi146 – 159Combined sources14
Helixi165 – 193Combined sources29
Turni213 – 215Combined sources3
Helixi233 – 248Combined sources16
Beta strandi335 – 337Combined sources3
Helixi338 – 355Combined sources18
Helixi359 – 363Combined sources5
Helixi374 – 381Combined sources8
Helixi385 – 400Combined sources16
Helixi423 – 437Combined sources15
Helixi445 – 468Combined sources24
Helixi483 – 581Combined sources99
Helixi583 – 586Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50c1-253[»]
c482-587[»]
5GMKelectron microscopy3.40c1-253[»]
c482-587[»]
5LJ3electron microscopy3.80O1-590[»]
5LJ5electron microscopy3.80O1-590[»]
5LQWelectron microscopy5.80W1-590[»]
5MPSelectron microscopy3.85O1-590[»]
5MQ0electron microscopy4.17O1-590[»]
5WSGelectron microscopy4.00c9-253[»]
c482-587[»]
6BK8electron microscopy3.30S1-590[»]
6EXNelectron microscopy3.70O1-590[»]
ProteinModelPortaliQ03654
SMRiQ03654
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 60HTH myb-type 1PROSITE-ProRule annotationAdd BLAST60
Domaini63 – 110HTH myb-type 2PROSITE-ProRule annotationAdd BLAST48

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni460 – 490Interaction with PRP19 and self-interactionAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi238 – 321Lys-richAdd BLAST84

Sequence similaritiesi

Belongs to the CEF1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074922
HOGENOMiHOG000111535
InParanoidiQ03654
KOiK12860
OMAiTISELMG
OrthoDBiEOG092C1DPM

Family and domain databases

CDDicd00167 SANT, 1 hit
InterProiView protein in InterPro
IPR021786 Cdc5p/Cef1
IPR009057 Homeobox-like_sf
IPR017930 Myb_dom
IPR001005 SANT/Myb
PfamiView protein in Pfam
PF11831 Myb_Cef, 1 hit
PF00249 Myb_DNA-binding, 2 hits
SMARTiView protein in SMART
SM00717 SANT, 2 hits
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS51294 HTH_MYB, 2 hits

Sequencei

Sequence statusi: Complete.

Q03654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL
60 70 80 90 100
RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIADM MARPAQVCVE
110 120 130 140 150
RYNRLLESED SGGAALSTGV TDLKAGDINP NAETQMARPD NGDLEDEEKE
160 170 180 190 200
MLAEARARLL NTQGKKATRK IRERMLEESK RIAELQKRRE LKQAGINVAI
210 220 230 240 250
KKPKKKYGTD IDYNEDIVYE QAPMPGIYDT STEDRQIKKK FEQFERKVNR
260 270 280 290 300
KGLDGNKDKP SKKNKDKKRK HDENEHVEKA ALGESTTLTD EYKKPKLILS
310 320 330 340 350
APGTKQGKVT YKKKLESKRQ KLIEAQATGT VLTPKELLPH DSGQEDNERS
360 370 380 390 400
NIKSGKQLKS RIRKFLVQMF ASLPSPKNDF EIVLSEDEKE EDAEIAEYEK
410 420 430 440 450
EFENERAMNE EDNFIEPPSQ NDAPRVSLVA VPLAYSTLPI PEFKNNPQSA
460 470 480 490 500
IDNKYNLLVA NAINKEPHMV PEDTVDFLKE VESRMQHITQ GRTSMKIQFK
510 520 530 540 550
TAMPPTEVLL ESIQSKVESI EQLQRKLQHV QPLEQQNNEM CSTLCHHSLP
560 570 580 590
ALIEGQRKYY ADYYAYRQEI RSLEGRRKRL QAMLNSSSSI
Length:590
Mass (Da):67,731
Last modified:November 1, 1997 - v1
Checksum:iA4C417A8B330EA9C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11G → E AA sequence (PubMed:10092627).Curated1
Sequence conflicti192K → E in AAT93161 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49809 Genomic DNA Translation: CAA89928.1
AY693142 Genomic DNA Translation: AAT93161.1
BK006946 Genomic DNA Translation: DAA10112.1
PIRiS55095
RefSeqiNP_013940.1, NM_001182720.1

Genome annotation databases

EnsemblFungiiYMR213W; YMR213W; YMR213W
GeneIDi855253
KEGGisce:YMR213W

Similar proteinsi

Entry informationi

Entry nameiCEF1_YEAST
AccessioniPrimary (citable) accession number: Q03654
Secondary accession number(s): D6W038, Q6B1D8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health