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Protein

Pre-mRNA-splicing factor CEF1

Gene

CEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing and cell cycle control. Required for the binding of the NTC complex (or PRP19-associated complex) components to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. Its absence leads to an arrest of the cell cycle, possibly due to the inefficient splicing of TUB1.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi33 – 5624H-T-H motifPROSITE-ProRule annotationAdd
BLAST
DNA bindingi84 – 10623H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. generation of catalytic spliceosome for second transesterification step Source: SGD
  2. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32896-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CEF1
Alternative name(s):
PRP nineteen-associated complex protein 85
PRP19-associated complex protein 85
Gene namesi
Name:CEF1
Synonyms:NTC85
Ordered Locus Names:YMR213W
ORF Names:YM8261.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR213w.
SGDiS000004826. CEF1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. Prp19 complex Source: SGD
  3. U2-type catalytic step 1 spliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi33 – 331W → G: No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-52 and G-84. Complete loss of function; when associated with G-52; G-84 and G-102. 1 Publication
Mutagenesisi52 – 521W → G: No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-33 and G-84. Complete loss of function; when associated with G-33; G-84 and G-102. 2 Publications
Mutagenesisi84 – 841W → G: No effect. Slower growth and thermosensitivity; when associated with G-33 or G-52. Complete loss of function; when associated with G-33 and G-52 or G-52 and Y-102. Complete loss of function; when associated with G-33; G-52 and G-102. 2 Publications
Mutagenesisi102 – 1021Y → G: No effect. Slower growth and thermosensitivity; when associated with G-52 or G-84. Complete loss of function; when associated with G-33; G-52 and G-84. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590Pre-mRNA-splicing factor CEF1PRO_0000197107Add
BLAST

Proteomic databases

MaxQBiQ03654.
PaxDbiQ03654.

Expressioni

Gene expression databases

GenevestigatoriQ03654.

Interactioni

Subunit structurei

Belongs to the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts with CLF1, ISY1, NTC20, PRP19, PRP46, SYF1 and SYF2.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUD31P253375EBI-476,EBI-547
CLF1Q123099EBI-476,EBI-484
CWC15Q037723EBI-476,EBI-36780
NTC20P383024EBI-476,EBI-20921
PRP19P325238EBI-476,EBI-493
SPP382Q064113EBI-476,EBI-576
SYF1Q040485EBI-476,EBI-540
SYF2P532773EBI-476,EBI-23308

Protein-protein interaction databases

BioGridi35391. 72 interactions.
DIPiDIP-1113N.
IntActiQ03654. 51 interactions.
MINTiMINT-517325.
STRINGi4932.YMR213W.

Structurei

3D structure databases

ProteinModelPortaliQ03654.
SMRiQ03654. Positions 12-119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6060HTH myb-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini63 – 11048HTH myb-type 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni460 – 49031Interaction with PRP19 and self-interactionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi238 – 32184Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the CEF1 family.Curated
Contains 2 HTH myb-type DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5147.
GeneTreeiENSGT00550000074922.
HOGENOMiHOG000111535.
InParanoidiQ03654.
KOiK12860.
OMAiTRENERT.
OrthoDBiEOG78SQWR.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR021786. DUF3351.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF11831. Myb_Cef. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51294. HTH_MYB. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL
60 70 80 90 100
RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIADM MARPAQVCVE
110 120 130 140 150
RYNRLLESED SGGAALSTGV TDLKAGDINP NAETQMARPD NGDLEDEEKE
160 170 180 190 200
MLAEARARLL NTQGKKATRK IRERMLEESK RIAELQKRRE LKQAGINVAI
210 220 230 240 250
KKPKKKYGTD IDYNEDIVYE QAPMPGIYDT STEDRQIKKK FEQFERKVNR
260 270 280 290 300
KGLDGNKDKP SKKNKDKKRK HDENEHVEKA ALGESTTLTD EYKKPKLILS
310 320 330 340 350
APGTKQGKVT YKKKLESKRQ KLIEAQATGT VLTPKELLPH DSGQEDNERS
360 370 380 390 400
NIKSGKQLKS RIRKFLVQMF ASLPSPKNDF EIVLSEDEKE EDAEIAEYEK
410 420 430 440 450
EFENERAMNE EDNFIEPPSQ NDAPRVSLVA VPLAYSTLPI PEFKNNPQSA
460 470 480 490 500
IDNKYNLLVA NAINKEPHMV PEDTVDFLKE VESRMQHITQ GRTSMKIQFK
510 520 530 540 550
TAMPPTEVLL ESIQSKVESI EQLQRKLQHV QPLEQQNNEM CSTLCHHSLP
560 570 580 590
ALIEGQRKYY ADYYAYRQEI RSLEGRRKRL QAMLNSSSSI
Length:590
Mass (Da):67,731
Last modified:November 1, 1997 - v1
Checksum:iA4C417A8B330EA9C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111G → E AA sequence (PubMed:10092627).Curated
Sequence conflicti192 – 1921K → E in AAT93161 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49809 Genomic DNA. Translation: CAA89928.1.
AY693142 Genomic DNA. Translation: AAT93161.1.
BK006946 Genomic DNA. Translation: DAA10112.1.
PIRiS55095.
RefSeqiNP_013940.1. NM_001182720.1.

Genome annotation databases

EnsemblFungiiYMR213W; YMR213W; YMR213W.
GeneIDi855253.
KEGGisce:YMR213W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49809 Genomic DNA. Translation: CAA89928.1.
AY693142 Genomic DNA. Translation: AAT93161.1.
BK006946 Genomic DNA. Translation: DAA10112.1.
PIRiS55095.
RefSeqiNP_013940.1. NM_001182720.1.

3D structure databases

ProteinModelPortaliQ03654.
SMRiQ03654. Positions 12-119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35391. 72 interactions.
DIPiDIP-1113N.
IntActiQ03654. 51 interactions.
MINTiMINT-517325.
STRINGi4932.YMR213W.

Proteomic databases

MaxQBiQ03654.
PaxDbiQ03654.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR213W; YMR213W; YMR213W.
GeneIDi855253.
KEGGisce:YMR213W.

Organism-specific databases

CYGDiYMR213w.
SGDiS000004826. CEF1.

Phylogenomic databases

eggNOGiCOG5147.
GeneTreeiENSGT00550000074922.
HOGENOMiHOG000111535.
InParanoidiQ03654.
KOiK12860.
OMAiTRENERT.
OrthoDBiEOG78SQWR.

Enzyme and pathway databases

BioCyciYEAST:G3O-32896-MONOMER.

Miscellaneous databases

NextBioi978831.
PROiQ03654.

Gene expression databases

GenevestigatoriQ03654.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR021786. DUF3351.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF11831. Myb_Cef. 1 hit.
PF00249. Myb_DNA-binding. 2 hits.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51294. HTH_MYB. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing."
    Tsai W.-Y., Chow Y.-T., Chen H.-R., Huang K.-T., Hong R.-I., Jan S.-P., Kuo N.-Y., Tsao T.Y., Chen C.-H., Cheng S.-C.
    J. Biol. Chem. 274:9455-9462(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 3-16, FUNCTION, DOMAIN, IDENTIFICATION IN THE PRP19-ASSOCIATED COMPLEX.
  5. "Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae."
    Burns C.G., Ohi R., Mehta S., O'Toole E.T., Winey M., Clark T.A., Sugnet C.W., Ares M. Jr., Gould K.L.
    Mol. Cell. Biol. 22:801-815(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-52 AND TRP-84.
  6. "Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p."
    Chen H.-R., Jan S.-P., Tsao T.Y., Sheu Y.-J., Banroques J., Cheng S.-C.
    Mol. Cell. Biol. 18:2196-2204(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRP19, IDENTIFICATION IN THE PRP19-ASSOCIATED COMPLEX.
  7. "Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes."
    Ohi R., Feoktistova A., McCann S., Valentine V., Look A.T., Lipsick J.S., Gould K.L.
    Mol. Cell. Biol. 18:4097-4108(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-33; TRP-52; TRP-84 AND TYR-102.
  8. "Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing."
    Burns C.G., Ohi R., Krainer A.R., Gould K.L.
    Proc. Natl. Acad. Sci. U.S.A. 96:13789-13794(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae."
    Ben-Yehuda S., Dix I., Russell C.S., McGarvey M., Beggs J.D., Kupiec M.
    Genetics 156:1503-1517(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CLF1; ISY1; NTC20; SYF1 AND SYF2.
  10. "Identification and characterization of two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p."
    Chen C.-H., Tsai W.-Y., Chen H.-R., Wang C.-H., Cheng S.-C.
    J. Biol. Chem. 276:488-494(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ISY1; NTC20 AND PRP19.
  11. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  12. "Characterization of interactions among the Cef1p-Prp19p-associated splicing complex."
    Ohi M.D., Gould K.L.
    RNA 8:798-815(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRP19; PRP46 AND SYF1.
  13. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  14. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCEF1_YEAST
AccessioniPrimary (citable) accession number: Q03654
Secondary accession number(s): D6W038, Q6B1D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.