Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q03654

- CEF1_YEAST

UniProt

Q03654 - CEF1_YEAST

Protein

Pre-mRNA-splicing factor CEF1

Gene

CEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in pre-mRNA splicing and cell cycle control. Required for the binding of the NTC complex (or PRP19-associated complex) components to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. Its absence leads to an arrest of the cell cycle, possibly due to the inefficient splicing of TUB1.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi33 – 5624H-T-H motifPROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi84 – 10623H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. generation of catalytic spliceosome for second transesterification step Source: SGD
    3. mRNA splicing, via spliceosome Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32896-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor CEF1
    Alternative name(s):
    PRP nineteen-associated complex protein 85
    PRP19-associated complex protein 85
    Gene namesi
    Name:CEF1
    Synonyms:NTC85
    Ordered Locus Names:YMR213W
    ORF Names:YM8261.07
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR213w.
    SGDiS000004826. CEF1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. Prp19 complex Source: SGD
    3. U2-type catalytic step 1 spliceosome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi33 – 331W → G: No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-52 and G-84. Complete loss of function; when associated with G-52; G-84 and G-102. 1 Publication
    Mutagenesisi52 – 521W → G: No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-33 and G-84. Complete loss of function; when associated with G-33; G-84 and G-102. 2 Publications
    Mutagenesisi84 – 841W → G: No effect. Slower growth and thermosensitivity; when associated with G-33 or G-52. Complete loss of function; when associated with G-33 and G-52 or G-52 and Y-102. Complete loss of function; when associated with G-33; G-52 and G-102. 2 Publications
    Mutagenesisi102 – 1021Y → G: No effect. Slower growth and thermosensitivity; when associated with G-52 or G-84. Complete loss of function; when associated with G-33; G-52 and G-84. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 590590Pre-mRNA-splicing factor CEF1PRO_0000197107Add
    BLAST

    Proteomic databases

    MaxQBiQ03654.
    PaxDbiQ03654.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03654.

    Interactioni

    Subunit structurei

    Belongs to the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts with CLF1, ISY1, NTC20, PRP19, PRP46, SYF1 and SYF2.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUD31P253375EBI-476,EBI-547
    CLF1Q123099EBI-476,EBI-484
    CWC15Q037723EBI-476,EBI-36780
    NTC20P383024EBI-476,EBI-20921
    PRP19P325238EBI-476,EBI-493
    SPP382Q064113EBI-476,EBI-576
    SYF1Q040485EBI-476,EBI-540
    SYF2P532773EBI-476,EBI-23308

    Protein-protein interaction databases

    BioGridi35391. 72 interactions.
    DIPiDIP-1113N.
    IntActiQ03654. 51 interactions.
    MINTiMINT-517325.
    STRINGi4932.YMR213W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03654.
    SMRiQ03654. Positions 12-119.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 6060HTH myb-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini63 – 11048HTH myb-type 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni460 – 49031Interaction with PRP19 and self-interactionAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi238 – 32184Lys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CEF1 family.Curated
    Contains 2 HTH myb-type DNA-binding domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5147.
    GeneTreeiENSGT00550000074922.
    HOGENOMiHOG000111535.
    KOiK12860.
    OMAiWNEYLNP.
    OrthoDBiEOG78SQWR.

    Family and domain databases

    Gene3Di1.10.10.60. 2 hits.
    InterProiIPR021786. DUF3351.
    IPR009057. Homeodomain-like.
    IPR017930. Myb_dom.
    IPR001005. SANT/Myb.
    [Graphical view]
    PfamiPF11831. Myb_Cef. 1 hit.
    PF00249. Myb_DNA-binding. 2 hits.
    [Graphical view]
    SMARTiSM00717. SANT. 2 hits.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS51294. HTH_MYB. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03654-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL    50
    RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIADM MARPAQVCVE 100
    RYNRLLESED SGGAALSTGV TDLKAGDINP NAETQMARPD NGDLEDEEKE 150
    MLAEARARLL NTQGKKATRK IRERMLEESK RIAELQKRRE LKQAGINVAI 200
    KKPKKKYGTD IDYNEDIVYE QAPMPGIYDT STEDRQIKKK FEQFERKVNR 250
    KGLDGNKDKP SKKNKDKKRK HDENEHVEKA ALGESTTLTD EYKKPKLILS 300
    APGTKQGKVT YKKKLESKRQ KLIEAQATGT VLTPKELLPH DSGQEDNERS 350
    NIKSGKQLKS RIRKFLVQMF ASLPSPKNDF EIVLSEDEKE EDAEIAEYEK 400
    EFENERAMNE EDNFIEPPSQ NDAPRVSLVA VPLAYSTLPI PEFKNNPQSA 450
    IDNKYNLLVA NAINKEPHMV PEDTVDFLKE VESRMQHITQ GRTSMKIQFK 500
    TAMPPTEVLL ESIQSKVESI EQLQRKLQHV QPLEQQNNEM CSTLCHHSLP 550
    ALIEGQRKYY ADYYAYRQEI RSLEGRRKRL QAMLNSSSSI 590
    Length:590
    Mass (Da):67,731
    Last modified:November 1, 1997 - v1
    Checksum:iA4C417A8B330EA9C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111G → E AA sequence (PubMed:10092627)Curated
    Sequence conflicti192 – 1921K → E in AAT93161. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49809 Genomic DNA. Translation: CAA89928.1.
    AY693142 Genomic DNA. Translation: AAT93161.1.
    BK006946 Genomic DNA. Translation: DAA10112.1.
    PIRiS55095.
    RefSeqiNP_013940.1. NM_001182720.1.

    Genome annotation databases

    EnsemblFungiiYMR213W; YMR213W; YMR213W.
    GeneIDi855253.
    KEGGisce:YMR213W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49809 Genomic DNA. Translation: CAA89928.1 .
    AY693142 Genomic DNA. Translation: AAT93161.1 .
    BK006946 Genomic DNA. Translation: DAA10112.1 .
    PIRi S55095.
    RefSeqi NP_013940.1. NM_001182720.1.

    3D structure databases

    ProteinModelPortali Q03654.
    SMRi Q03654. Positions 12-119.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35391. 72 interactions.
    DIPi DIP-1113N.
    IntActi Q03654. 51 interactions.
    MINTi MINT-517325.
    STRINGi 4932.YMR213W.

    Proteomic databases

    MaxQBi Q03654.
    PaxDbi Q03654.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR213W ; YMR213W ; YMR213W .
    GeneIDi 855253.
    KEGGi sce:YMR213W.

    Organism-specific databases

    CYGDi YMR213w.
    SGDi S000004826. CEF1.

    Phylogenomic databases

    eggNOGi COG5147.
    GeneTreei ENSGT00550000074922.
    HOGENOMi HOG000111535.
    KOi K12860.
    OMAi WNEYLNP.
    OrthoDBi EOG78SQWR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32896-MONOMER.

    Miscellaneous databases

    NextBioi 978831.
    PROi Q03654.

    Gene expression databases

    Genevestigatori Q03654.

    Family and domain databases

    Gene3Di 1.10.10.60. 2 hits.
    InterProi IPR021786. DUF3351.
    IPR009057. Homeodomain-like.
    IPR017930. Myb_dom.
    IPR001005. SANT/Myb.
    [Graphical view ]
    Pfami PF11831. Myb_Cef. 1 hit.
    PF00249. Myb_DNA-binding. 2 hits.
    [Graphical view ]
    SMARTi SM00717. SANT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS51294. HTH_MYB. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing."
      Tsai W.-Y., Chow Y.-T., Chen H.-R., Huang K.-T., Hong R.-I., Jan S.-P., Kuo N.-Y., Tsao T.Y., Chen C.-H., Cheng S.-C.
      J. Biol. Chem. 274:9455-9462(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 3-16, FUNCTION, DOMAIN, IDENTIFICATION IN THE PRP19-ASSOCIATED COMPLEX.
    5. "Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae."
      Burns C.G., Ohi R., Mehta S., O'Toole E.T., Winey M., Clark T.A., Sugnet C.W., Ares M. Jr., Gould K.L.
      Mol. Cell. Biol. 22:801-815(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TRP-52 AND TRP-84.
    6. "Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p."
      Chen H.-R., Jan S.-P., Tsao T.Y., Sheu Y.-J., Banroques J., Cheng S.-C.
      Mol. Cell. Biol. 18:2196-2204(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRP19, IDENTIFICATION IN THE PRP19-ASSOCIATED COMPLEX.
    7. "Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes."
      Ohi R., Feoktistova A., McCann S., Valentine V., Look A.T., Lipsick J.S., Gould K.L.
      Mol. Cell. Biol. 18:4097-4108(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TRP-33; TRP-52; TRP-84 AND TYR-102.
    8. "Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing."
      Burns C.G., Ohi R., Krainer A.R., Gould K.L.
      Proc. Natl. Acad. Sci. U.S.A. 96:13789-13794(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae."
      Ben-Yehuda S., Dix I., Russell C.S., McGarvey M., Beggs J.D., Kupiec M.
      Genetics 156:1503-1517(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLF1; ISY1; NTC20; SYF1 AND SYF2.
    10. "Identification and characterization of two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p."
      Chen C.-H., Tsai W.-Y., Chen H.-R., Wang C.-H., Cheng S.-C.
      J. Biol. Chem. 276:488-494(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ISY1; NTC20 AND PRP19.
    11. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    12. "Characterization of interactions among the Cef1p-Prp19p-associated splicing complex."
      Ohi M.D., Gould K.L.
      RNA 8:798-815(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRP19; PRP46 AND SYF1.
    13. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    14. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCEF1_YEAST
    AccessioniPrimary (citable) accession number: Q03654
    Secondary accession number(s): D6W038, Q6B1D8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 784 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3