Q03647 (MEL_SACPS) Reviewed, UniProtKB/Swiss-Prot
Last modified
September 21, 2011.
Version 55.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Alpha-galactosidase EC=3.2.1.22 Alternative name(s): Alpha-D-galactoside galactohydrolase MELx Melibiase | ||
| Gene names |
| ||
| Organism | Saccharomyces pastorianus (Lager yeast) (Saccharomyces carlsbergensis) | ||
| Taxonomic identifier | 27292 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 471 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | Secreted By similarity. |
| Sequence similarities | Belongs to the glycosyl hydrolase 27 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond Glycoprotein |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | cation binding Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 18 | 18 | By similarity | ||||||||
| Chain | 19 – 471 | 453 | Alpha-galactosidase | PRO_0000001009 | |||||||
Sites | |||||||||||
| Active site | 149 | 1 | Nucleophile By similarity | ||||||||
| Active site | 209 | 1 | Proton donor By similarity | ||||||||
| Binding site | 72 | 1 | Substrate By similarity | ||||||||
| Binding site | 73 | 1 | Substrate By similarity | ||||||||
| Binding site | 147 | 1 | Substrate By similarity | ||||||||
| Binding site | 205 | 1 | Substrate By similarity | ||||||||
| Binding site | 251 | 1 | Substrate By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 82 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 175 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 270 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 403 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 412 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 417 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 422 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 435 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 454 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 42 ↔ 74 | By similarity | |||||||||
| Disulfide bond | 121 ↔ 151 | By similarity | |||||||||
| Disulfide bond | 221 ↔ 237 | By similarity | |||||||||
| Disulfide bond | 223 ↔ 230 | By similarity | |||||||||
Sequences
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References
| [1] | "Cloning sequence and chromosomal location of a MEL gene from Saccharomyces carlsbergenesis NCYC396." Turakainen H., Korhola M., Aho S. Gene 101:97-104(1991) [PubMed: 1711992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCYC 396. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M58484 Genomic DNA. Translation: AAA34769.1. |
| PIR | JQ1021. |
3D structure databases | |
| ProteinModelPortal | Q03647. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH27. Glycoside Hydrolase Family 27. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR006215. Glyco_hydro_melibiase. IPR017853. Glycoside_hydrolase_SF. [Graphical view] |
| Gene3D | G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. |
| Pfam | PF02065. Melibiase. 2 hits. [Graphical view] |
| PRINTS | PR00740. GLHYDRLASE27. PR00748. MELIBIASE. |
| SUPFAM | SSF51445. Glyco_hydro_cat. 1 hit. |
| PROSITE | PS00512. ALPHA_GALACTOSIDASE. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MEL_SACPS | ||||||||
| Accession | Primary (citable) accession number: Q03647 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with