Reviewed, UniProtKB/Swiss-Prot Q03647 (MEL_SACPS)

Names and origin

Protein names

Recommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Melibiase
    MELx

Gene names

Name:

MEL

Organism

Saccharomyces pastorianus (Lager yeast) (Saccharomyces carlsbergensis)

Taxonomic identifier

27292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Chain	19 – 471	453	Alpha-galactosidase		PRO_0000001009
Sites
	Active site	149	1	Nucleophile By similarity
	Active site	209	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	175	1	N-linked (GlcNAc...) Potential
	Glycosylation	270	1	N-linked (GlcNAc...) Potential
	Glycosylation	403	1	N-linked (GlcNAc...) Potential
	Glycosylation	412	1	N-linked (GlcNAc...) Potential
	Glycosylation	417	1	N-linked (GlcNAc...) Potential
	Glycosylation	422	1	N-linked (GlcNAc...) Potential
	Glycosylation	435	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 74		By similarity
	Disulfide bond	121 ↔ 151		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03647-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E49E27EC278C4A23	FASTA	471	52,000
	10 20 30 40 50 60 MFLLYLFTSF AAVSGVLGSS PSYNGLGLTP QMGWDNWNTF ACDVSEQLLL DTADRISEIG 70 80 90 100 110 120 LKDLGYTYVI LDDCWSSGRT ANGTLVADKE KFPNGMSHVA DHLHNNNFLF GMYSSAGEYT 130 140 150 160 170 180 CAGYPGSLGH EEEDAEFFAS NGVDYLKYDN CYNKGQFGAP ETSYKRYKAM SDALNKTGRP 190 200 210 220 230 240 IFYSLCNWGQ DLTHYWGSDI ANSWRMSGDI YPQFTRPDSR CPCDGDQFDC AYAGFHCSIM 250 260 270 280 290 300 NILNKAAPMG QNAGIGGWND LDNLEVGVGN LTDDEEKAHF SMWAMVKSPL VIGADVNHLK 310 320 330 340 350 360 ASSYSIYSQA SVIAINQDPK GVPATRVWRH QVPQTDKYGQ GEIQFWSGPL DNGDQVIALL 370 380 390 400 410 420 NGGIKPRPMN TNLEEIFFDS YLGFEQLSSN WDIYDLWANR VDNATSANIL NNNSVGNATI 430 440 450 460 470 YNATALSYKD GMAKNDTRLF GTKIGSISPD GLLNTTVPAH GIAFYRLRRS T

References

[1]	"Cloning sequence and chromosomal location of a MEL gene from Saccharomyces carlsbergenesis NCYC396." Turakainen H., Korhola M., Aho S. Gene 101:97-104(1991) [PubMed: 1711992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCYC 396.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58484 Genomic DNA. Translation: AAA34769.1.
PIR	JQ1021.
3D structure databases
SMR	Q03647. Positions 24-399.
ModBase	Search...
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
Enzyme and pathway databases
BRENDA	3.2.1.22. 3401.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002241. Glyco_hydro_27. IPR017853. Glyco_hydro_catalytic_core. IPR000111. Glyco_hydro_GHD. IPR006215. Glyco_hydro_melibiase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF02065. Melibiase. 2 hits. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27. PR00748. MELIBIASE.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MEL_SACPS

Accession

Primary (citable) accession number: Q03647

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)