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Q03640

- TCB3_YEAST

UniProt

Q03640 - TCB3_YEAST

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Protein
Tricalbin-3
Gene
TCB3, YML072C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play a role in membrane trafficking.2 Publications

Cofactori

Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1150 – 11501Calcium 1 By similarity
Metal bindingi1150 – 11501Calcium 2 By similarity
Metal bindingi1156 – 11561Calcium 1 By similarity
Metal bindingi1204 – 12041Calcium 1 By similarity
Metal bindingi1204 – 12041Calcium 2 By similarity
Metal bindingi1206 – 12061Calcium 1 By similarity
Metal bindingi1206 – 12061Calcium 2 By similarity
Metal bindingi1206 – 12061Calcium 3 By similarity
Metal bindingi1212 – 12121Calcium 2 By similarity
Metal bindingi1212 – 12121Calcium 3 By similarity

GO - Molecular functioni

  1. calcium-dependent phospholipid binding Source: UniProtKB-KW
  2. lipid binding Source: SGD
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. endoplasmic reticulum membrane organization Source: SGD
  2. regulation of phosphatidylinositol dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding, Lipid-binding, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32666-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tricalbin-3
Gene namesi
Name:TCB3
Ordered Locus Names:YML072C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML072c.
SGDiS000004537. TCB3.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 206206Cytoplasmic By similarity
Add
BLAST
Transmembranei207 – 22721Helical; Reviewed prediction
Add
BLAST
Topological domaini228 – 2281Extracellular By similarity
Transmembranei229 – 24921Helical; Reviewed prediction
Add
BLAST
Topological domaini250 – 15451296Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. cellular bud Source: SGD
  2. cortical endoplasmic reticulum Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15451545Tricalbin-3
PRO_0000203249Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine1 Publication
Modified residuei112 – 1121Phosphoserine1 Publication
Modified residuei1340 – 13401Phosphoserine1 Publication
Modified residuei1342 – 13421Phosphoserine1 Publication
Modified residuei1346 – 13461Phosphoserine1 Publication
Modified residuei1350 – 13501Phosphothreonine4 Publications
Modified residuei1354 – 13541Phosphoserine1 Publication
Modified residuei1400 – 14001Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03640.
PaxDbiQ03640.
PeptideAtlasiQ03640.

Expressioni

Gene expression databases

GenevestigatoriQ03640.

Interactioni

Subunit structurei

Interacts with TCB2 via its C-terminal domain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-27872,EBI-27872
DIP5P533881EBI-27872,EBI-5904
DUR3P334131EBI-27872,EBI-2060312
FLC1Q089671EBI-27872,EBI-36673
FMP45Q076511EBI-27872,EBI-2051056
FTR1P400881EBI-27872,EBI-7138
GPA2P108231EBI-27872,EBI-7382
HNM1P198071EBI-27872,EBI-8409
HSP30P256191EBI-27872,EBI-8563
HXT1P324651EBI-27872,EBI-8759
HXT2P235851EBI-27872,EBI-2054100
HXT3P324661EBI-27872,EBI-8770
HXT5P386951EBI-27872,EBI-8778
HXT7P390041EBI-27872,EBI-8790
IST2P382501EBI-27872,EBI-21520
ITR1P306051EBI-27872,EBI-2050956
MEP1P402601EBI-27872,EBI-10714
MEP3P533901EBI-27872,EBI-10729
MID2P360271EBI-27872,EBI-10901
MUP1P502761EBI-27872,EBI-11624
OSH7P387551EBI-27872,EBI-12641
PDR12Q027851EBI-27872,EBI-13065
PHO84P252971EBI-27872,EBI-13320
PIS1P061971EBI-27872,EBI-13458
PLM2Q043831EBI-27872,EBI-2079237
PMP2P409751EBI-27872,EBI-2043041
PMP3P872841EBI-27872,EBI-13555
PST2Q123351EBI-27872,EBI-14064
QDR2P404741EBI-27872,EBI-25203
RFS1P382341EBI-27872,EBI-21445
RIM1P324451EBI-27872,EBI-15206
RSN1Q035161EBI-27872,EBI-27593
RTN1Q049471EBI-27872,EBI-38020
SDS23P531721EBI-27872,EBI-23782
SFK1P357351EBI-27872,EBI-26678
SNQ2P325681EBI-27872,EBI-17590
TCB1Q124661EBI-27872,EBI-34614
TCB2P482311EBI-27872,EBI-28779
TPO1Q078241EBI-27872,EBI-38106
TPO2P532831EBI-27872,EBI-2044753
TPO3Q064511EBI-27872,EBI-34275
TPO4Q122561EBI-27872,EBI-37213
VHT1P532411EBI-27872,EBI-20297
WSC2P538321EBI-27872,EBI-2055941
WSC3Q122151EBI-27872,EBI-20551
YBL029C-AQ3E7561EBI-27872,EBI-2044812
YLR326WQ061701EBI-27872,EBI-35994
YLR413WQ066891EBI-27872,EBI-38659
YOP1Q124021EBI-27872,EBI-37092
YOR1P530491EBI-27872,EBI-29324

Protein-protein interaction databases

BioGridi35069. 88 interactions.
DIPiDIP-6589N.
IntActiQ03640. 62 interactions.
MINTiMINT-667993.
STRINGi4932.YML072C.

Structurei

3D structure databases

ProteinModelPortaliQ03640.
SMRiQ03640. Positions 478-736, 1053-1223.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini478 – 580103C2 1
Add
BLAST
Domaini785 – 88197C2 2
Add
BLAST
Domaini1121 – 121898C2 3
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili620 – 66041 Reviewed prediction
Add
BLAST
Coiled coili937 – 97236 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1316 – 140489Ser-rich
Add
BLAST

Domaini

The C-terminal C2 domain shows Ca2+-dependent phospholipid binding. It binds to phosphatidylserine, phosphatidylinositol and various phosphoinositides. The other C2 domains do not retain all 5 conserved Asp residues found in calcium-binding C2 domains.

Sequence similaritiesi

Belongs to the tricalbin family.
Contains 3 C2 domains.

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5038.
GeneTreeiENSGT00730000113103.
HOGENOMiHOG000093250.
OMAiFWVIYMP.
OrthoDBiEOG7TQV86.

Family and domain databases

Gene3Di2.60.40.150. 4 hits.
InterProiIPR000008. C2_dom.
IPR017147. Tricalbin.
[Graphical view]
PfamiPF00168. C2. 5 hits.
[Graphical view]
PIRSFiPIRSF037232. Tricalbin. 1 hit.
SMARTiSM00239. C2. 5 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 5 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03640-1 [UniParc]FASTAAdd to Basket

« Hide

MTGIKAQVHP PPDSTLFHEE EKKKVGGNLP QKVINQQERG SDHAPSGHHQ     50
YHQLINHDAN DTKTSNSVSD VSKGQKTADS NPEGKKQSSK DIFVASSAQK 100
TNQLPGPNPQ GSIGAVPLEG LRPKEFRSAP SRKPNKFDTS ITKPGVLDDL 150
GKLDEKDIKE KFHLDSDDKL FPWQNVGEFH ASGKGSPNTK MSRVIKAYIL 200
ENFYNDWYCN IATVLGTCFF SWLFAYIGFS WWSMIFIFLG TATVYNAEYT 250
RFNRNIRDDL KRVTVEETLS DRVESTTWLN SFLSKFWVIY MPVLSQQVKD 300
NVNPQLAGVA PGYGIDALAI DEFTLGSKAP TIKGIKSYTK TGKNTVEMDW 350
SFAFTPSDVS DMTATEAREK INPKISLGVT LGKSFVSKTM PILVEDINVA 400
GKMRIKVEFG KAFPNIKIVS LQLLEPPLID FALKPIGGDT LGLDVMSFLP 450
GLKSFVKNII NSNIGPMLFP PNHLDINVED IMAAQSKEAI GVLAVTIASA 500
DSLKGSDFIT NTVDPYIVMT TEDAVPGTDE EVRTSIKSNV KNPRWNETKY 550
LLLNTLEQKL NLKCFDFNDV RKDTVIGDLQ LDLADLLQNP VLDNQTAELR 600
SGTKSKGILH YSLHWFPVKE DKSEEKAVER AEAKAKGKKE DENEDTTEKE 650
EDENEESSQT DVGIAKITLQ KVKYLDTTSS MTGSLSPCAE LFIDGQKVKS 700
YRTLRRINEP SWNETIEVLV PSKSNSKFVL KIFDDRMNGK ALICEYSSSL 750
DDIMTTLDTA QEFVKGSPQG DIYLDVSWKS IEMTGAFAAA NSVSEPIGCI 800
KLDVKDAIIK GDLSGVGDVD PYYTVSLNRR VLYKSIYHSD TDHPIFDNST 850
YVPIFSPNQI LTLEFHDYQK IGKDRFIGSV QIPTSNVFKK DPKSGKYVGN 900
NGKEEISKLK LKDHEHKVTE SIVNVSTTFI PINLVYSPEE LVNVEKLEKE 950
LKEKKKKFEA TQEENEQEME KNPKEWEVAE IEDPFDSDEK KINRKAKLSL 1000
NELIKQKSGI LSMQILEGTL SPSSAYLEIL ADDISYPVFI CMKPSQGKLN 1050
SEMANIFIRD LNYSKLHFRV SKKHIAKDSD DVISETSYST LKLLKQAYEE 1100
PMWLNFNGSK MKVRFLYTPT SVKLPSSESV EDTGYLNIKL ISGHGLKSAD 1150
RNGYSDPFVH IFVNDKKVFK SNIKKKTLDP VWNEDAKIPI LSRSKNQVIF 1200
NVLDWDRAGD NDDLGQASLD VSSLEVGKTY NWNLNLNTQG SIKLQGSFNP 1250
EYIKPSFDIV KGGITDKPMK IASGAAHATV GIAGTGIGAA TGVATGGLKK 1300
GGHLLKSLGG NPMKRSKSSN GNESNGAKKS SEKKSFDRRS PSNLNSTSVT 1350
PRASLDYDPS VPNTSYAPVQ SASPVVKPTD NTSSSSNKKD TPSSNSRGHS 1400
RASSFARTLA PHGTYNGFIT VVAAENVAKH VQIKISLTQG GRLKHIYKTK 1450
SQKANNDGVA VFDEECSFKA SPEANLVLGA ISHQRLSRDK DLGIAQINLG 1500
DPQIQQDGQI SVKLGDGHLI VKINYGKDKN GQVPPVPEVP QEYTQ 1545
Length:1,545
Mass (Da):171,076
Last modified:November 1, 1997 - v1
Checksum:i77A0AE57F9259784
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46373 Genomic DNA. Translation: CAA86506.1.
BK006946 Genomic DNA. Translation: DAA09825.1.
PIRiS48824.
RefSeqiNP_013639.1. NM_001182431.1.

Genome annotation databases

EnsemblFungiiYML072C; YML072C; YML072C.
GeneIDi854903.
KEGGisce:YML072C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46373 Genomic DNA. Translation: CAA86506.1 .
BK006946 Genomic DNA. Translation: DAA09825.1 .
PIRi S48824.
RefSeqi NP_013639.1. NM_001182431.1.

3D structure databases

ProteinModelPortali Q03640.
SMRi Q03640. Positions 478-736, 1053-1223.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35069. 88 interactions.
DIPi DIP-6589N.
IntActi Q03640. 62 interactions.
MINTi MINT-667993.
STRINGi 4932.YML072C.

Proteomic databases

MaxQBi Q03640.
PaxDbi Q03640.
PeptideAtlasi Q03640.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML072C ; YML072C ; YML072C .
GeneIDi 854903.
KEGGi sce:YML072C.

Organism-specific databases

CYGDi YML072c.
SGDi S000004537. TCB3.

Phylogenomic databases

eggNOGi COG5038.
GeneTreei ENSGT00730000113103.
HOGENOMi HOG000093250.
OMAi FWVIYMP.
OrthoDBi EOG7TQV86.

Enzyme and pathway databases

BioCyci YEAST:G3O-32666-MONOMER.

Miscellaneous databases

NextBioi 977885.

Gene expression databases

Genevestigatori Q03640.

Family and domain databases

Gene3Di 2.60.40.150. 4 hits.
InterProi IPR000008. C2_dom.
IPR017147. Tricalbin.
[Graphical view ]
Pfami PF00168. C2. 5 hits.
[Graphical view ]
PIRSFi PIRSF037232. Tricalbin. 1 hit.
SMARTi SM00239. C2. 5 hits.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 5 hits.
PROSITEi PS50004. C2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "The tricalbin C2 domains: lipid-binding properties of a novel, synaptotagmin-like yeast protein family."
    Schulz T.A., Creutz C.E.
    Biochemistry 43:3987-3995(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CALCIUM-DEPENDENT BINDING TO PHOSPHOLIPIDS.
  6. "Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking."
    Creutz C.E., Snyder S.L., Schulz T.A.
    Cell. Mol. Life Sci. 61:1208-1220(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TCB2.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67; SER-112 AND THR-1350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1340; SER-1342; SER-1346; THR-1350; SER-1354 AND SER-1400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTCB3_YEAST
AccessioniPrimary (citable) accession number: Q03640
Secondary accession number(s): D6VZA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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