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Q03631

- WAR1_YEAST

UniProt

Q03631 - WAR1_YEAST

Protein

Weak acid resistance protein 1

Gene

WAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    transcription factor which binds to a weak acid response element (WARE) to mediate stress induction of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively.5 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi76 – 10934Zn(2)-C6 fungal-typeAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: SGD
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. response to acid chemical Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32668-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Weak acid resistance protein 1
    Gene namesi
    Name:WAR1
    Ordered Locus Names:YML076C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML076c.
    SGDiS000004541. WAR1.

    Subcellular locationi

    Nucleus 3 Publications

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi368 – 3681S → L: Causes hyperactivity with constitutive induction of PDR12. 1 Publication
    Mutagenesisi452 – 4521Y → C: Causes hyperactivity with constitutive induction of PDR12. 1 Publication
    Mutagenesisi463 – 4631Y → C: Causes hyperactivity with constitutive induction of PDR12. 1 Publication
    Mutagenesisi640 – 6401A → T: Causes hyperactivity with constitutive induction of PDR12. 1 Publication
    Mutagenesisi703 – 7031S → P: Causes hyperactivitywith constitutive induction of PDR12. 1 Publication
    Mutagenesisi762 – 7621K → R or N: Causes hyperactivity with constitutive induction of PDR12. 2 Publications
    Mutagenesisi763 – 7631F → M: Causes hyperactivity with constitutive induction of PDR12. 1 Publication
    Mutagenesisi764 – 7641Missing: Causes hypersensitivity to sorbate through its inability to induce PDR12. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 944944Weak acid resistance protein 1PRO_0000115004Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281Phosphothreonine2 Publications

    Post-translational modificationi

    Phosphorylation is required for PDR12 induction.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03631.
    PaxDbiQ03631.
    PeptideAtlasiQ03631.
    PRIDEiQ03631.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03631.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi35065. 16 interactions.
    MINTiMINT-2734616.
    STRINGi4932.YML076C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03631.
    SMRiQ03631. Positions 74-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG46074.
    HOGENOMiHOG000142350.
    OMAiNQTRKRR.
    OrthoDBiEOG7Q2NDP.

    Family and domain databases

    InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view]
    SMARTiSM00066. GAL4. 1 hit.
    [Graphical view]
    SUPFAMiSSF57701. SSF57701. 1 hit.
    PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03631-1 [UniParc]FASTAAdd to Basket

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    MDTQIAITGV AVGKEINNDN SKTDQKVSLP KADVPCIDKA TQTIIEGCSK    50
    DDPRLSYPTK LETTEKGKTK RNSFACVCCH SLKQKCEPSD VNDIYRKPCR 100
    RCLKHKKLCK FDLSKRTRKR KPRSRSPTPF ESPMVNVSTK SKGPTDSEES 150
    SLKDGTSYLA SFPSDPNAKQ FPNSRTVLPG LQQSLSDLWS TLSQPPSYGA 200
    REAETTSTGE ITTNNHTKSN GSVPTNPAVL ASNDEHTNIS DAPVIYSTYN 250
    SPVPISSAPT SINSEALFKH RPKIVGDEET QNVKVKRQKK SYSRHMTRSF 300
    RKQLQSLIIS QKGKIRDISM KLDTWSKQWN DLVEKSMFLP TIADPVSVGI 350
    ISHEEATLRL HLYKTEISYL SKLPFIKVEE NVSVDELRKK KPILFSVIMS 400
    CVSIVLTPKQ TTRGTIMKLD SFVLNLITNQ IFKANNKSIE IIESLSTLCL 450
    WYNFFEWSSK TRYHIFNYIC CCLTRDLGPT YVNRSFGMFS DEDPKRFKSP 500
    LELYSNGASL TLLVYISALN ISIFLRQSIQ ARWSHVTEKA CEDLVKETKK 550
    SRHYDNDKLL LDSADDPILV QFAKMNHVLE NIHTHLHERD LNDDEFDDPI 600
    FTKKYLNKLM EKYHKQLQEI FTKLDRNRPR VIAFYYSVEA YLYQYKLAVF 650
    IGEMSHTINE KVELPREIMD DFVKCYHCCK SALEEFSKLE PILITSLPLF 700
    HTSRIIYTVG MLLLKLRYSV VAIPSFHDLM PLTDDAIALV IGVNNLLEKT 750
    SELYPFNNSL YKFRYVIALF CQTYANKVID VADRYNAERE KLKEKQVIDE 800
    VSNGHDGTKP INAYVTESQK MPTEEDPIID NNTNQNITAV PDEMLPVYSR 850
    VRDDTAAMNL NINSTSYMNE SPHEHRESMT GTTLLPPPFI SNDVTNSADS 900
    TNIKPSPSSS VDNLNDYLTD INSLAWGVNS LNDEFWTDLF MNDI 944
    Length:944
    Mass (Da):107,561
    Last modified:November 1, 1997 - v1
    Checksum:i020A56745DF52CCC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46373 Genomic DNA. Translation: CAA86502.1.
    BK006946 Genomic DNA. Translation: DAA09821.1.
    PIRiS48821.
    RefSeqiNP_013635.1. NM_001182435.1.

    Genome annotation databases

    EnsemblFungiiYML076C; YML076C; YML076C.
    GeneIDi854899.
    KEGGisce:YML076C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46373 Genomic DNA. Translation: CAA86502.1 .
    BK006946 Genomic DNA. Translation: DAA09821.1 .
    PIRi S48821.
    RefSeqi NP_013635.1. NM_001182435.1.

    3D structure databases

    ProteinModelPortali Q03631.
    SMRi Q03631. Positions 74-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35065. 16 interactions.
    MINTi MINT-2734616.
    STRINGi 4932.YML076C.

    Proteomic databases

    MaxQBi Q03631.
    PaxDbi Q03631.
    PeptideAtlasi Q03631.
    PRIDEi Q03631.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML076C ; YML076C ; YML076C .
    GeneIDi 854899.
    KEGGi sce:YML076C.

    Organism-specific databases

    CYGDi YML076c.
    SGDi S000004541. WAR1.

    Phylogenomic databases

    eggNOGi NOG46074.
    HOGENOMi HOG000142350.
    OMAi NQTRKRR.
    OrthoDBi EOG7Q2NDP.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32668-MONOMER.

    Miscellaneous databases

    NextBioi 977873.

    Gene expression databases

    Genevestigatori Q03631.

    Family and domain databases

    InterProi IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view ]
    SMARTi SM00066. GAL4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57701. SSF57701. 1 hit.
    PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "War1p, a novel transcription factor controlling weak acid stress response in yeast."
      Kren A., Mamnun Y.M., Bauer B.E., Schueller C., Wolfger H., Hatzixanthis K., Mollapour M., Gregori C., Piper P.W., Kuchler K.
      Mol. Cell. Biol. 23:1775-1785(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, PHOSPHORYLATION.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae."
      Schueller C., Mamnun Y.M., Mollapour M., Krapf G., Schuster M., Bauer B.E., Piper P.W., Kuchler K.
      Mol. Biol. Cell 15:706-720(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A new physiological role for Pdr12p in Saccharomyces cerevisiae: export of aromatic and branched-chain organic acids produced in amino acid catabolism."
      Hazelwood L.A., Tai S.L., Boer V.M., de Winde J.H., Pronk J.T., Daran J.-M.
      FEMS Yeast Res. 6:937-945(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "A genetic screen identifies mutations in the yeast WAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation."
      Gregori C., Bauer B.E., Schwartz C., Kren A., Schueller C., Kuchler K.
      FEBS J. 274:3094-3107(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-762; PHE-763 AND ARG-764.
    9. "Weak organic acids trigger conformational changes of the yeast transcription factor War1 in vivo to elicit stress adaptation."
      Gregori C., Schueller C., Frohner I.E., Ammerer G., Kuchler K.
      J. Biol. Chem. 283:25752-25764(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-368; TYR-452; TYR-463; ALA-640; SER-703 AND LYS-762.
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-128, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiWAR1_YEAST
    AccessioniPrimary (citable) accession number: Q03631
    Secondary accession number(s): D6W0K7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 907 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3