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Q03631 (WAR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Weak acid resistance protein 1
Gene names
Name:WAR1
Ordered Locus Names:YML076C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length944 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

transcription factor which binds to a weak acid response element (WARE) to mediate stress induction of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively. Ref.3 Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure

Homodimer. Ref.3

Subcellular location

Nucleus Ref.3 Ref.4 Ref.8.

Post-translational modification

Phosphorylation is required for PDR12 induction.

Miscellaneous

Present with 907 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 944944Weak acid resistance protein 1
PRO_0000115004

Regions

DNA binding76 – 10934Zn(2)-C6 fungal-type

Amino acid modifications

Modified residue1281Phosphothreonine Ref.10

Experimental info

Mutagenesis3681S → L: Causes hyperactivity with constitutive induction of PDR12. Ref.9
Mutagenesis4521Y → C: Causes hyperactivity with constitutive induction of PDR12. Ref.9
Mutagenesis4631Y → C: Causes hyperactivity with constitutive induction of PDR12. Ref.9
Mutagenesis6401A → T: Causes hyperactivity with constitutive induction of PDR12. Ref.9
Mutagenesis7031S → P: Causes hyperactivitywith constitutive induction of PDR12. Ref.9
Mutagenesis7621K → R or N: Causes hyperactivity with constitutive induction of PDR12. Ref.8 Ref.9
Mutagenesis7631F → M: Causes hyperactivity with constitutive induction of PDR12. Ref.8
Mutagenesis7641Missing: Causes hypersensitivity to sorbate through its inability to induce PDR12. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q03631 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 020A56745DF52CCC

FASTA944107,561
        10         20         30         40         50         60 
MDTQIAITGV AVGKEINNDN SKTDQKVSLP KADVPCIDKA TQTIIEGCSK DDPRLSYPTK 

        70         80         90        100        110        120 
LETTEKGKTK RNSFACVCCH SLKQKCEPSD VNDIYRKPCR RCLKHKKLCK FDLSKRTRKR 

       130        140        150        160        170        180 
KPRSRSPTPF ESPMVNVSTK SKGPTDSEES SLKDGTSYLA SFPSDPNAKQ FPNSRTVLPG 

       190        200        210        220        230        240 
LQQSLSDLWS TLSQPPSYGA REAETTSTGE ITTNNHTKSN GSVPTNPAVL ASNDEHTNIS 

       250        260        270        280        290        300 
DAPVIYSTYN SPVPISSAPT SINSEALFKH RPKIVGDEET QNVKVKRQKK SYSRHMTRSF 

       310        320        330        340        350        360 
RKQLQSLIIS QKGKIRDISM KLDTWSKQWN DLVEKSMFLP TIADPVSVGI ISHEEATLRL 

       370        380        390        400        410        420 
HLYKTEISYL SKLPFIKVEE NVSVDELRKK KPILFSVIMS CVSIVLTPKQ TTRGTIMKLD 

       430        440        450        460        470        480 
SFVLNLITNQ IFKANNKSIE IIESLSTLCL WYNFFEWSSK TRYHIFNYIC CCLTRDLGPT 

       490        500        510        520        530        540 
YVNRSFGMFS DEDPKRFKSP LELYSNGASL TLLVYISALN ISIFLRQSIQ ARWSHVTEKA 

       550        560        570        580        590        600 
CEDLVKETKK SRHYDNDKLL LDSADDPILV QFAKMNHVLE NIHTHLHERD LNDDEFDDPI 

       610        620        630        640        650        660 
FTKKYLNKLM EKYHKQLQEI FTKLDRNRPR VIAFYYSVEA YLYQYKLAVF IGEMSHTINE 

       670        680        690        700        710        720 
KVELPREIMD DFVKCYHCCK SALEEFSKLE PILITSLPLF HTSRIIYTVG MLLLKLRYSV 

       730        740        750        760        770        780 
VAIPSFHDLM PLTDDAIALV IGVNNLLEKT SELYPFNNSL YKFRYVIALF CQTYANKVID 

       790        800        810        820        830        840 
VADRYNAERE KLKEKQVIDE VSNGHDGTKP INAYVTESQK MPTEEDPIID NNTNQNITAV 

       850        860        870        880        890        900 
PDEMLPVYSR VRDDTAAMNL NINSTSYMNE SPHEHRESMT GTTLLPPPFI SNDVTNSADS 

       910        920        930        940 
TNIKPSPSSS VDNLNDYLTD INSLAWGVNS LNDEFWTDLF MNDI 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"War1p, a novel transcription factor controlling weak acid stress response in yeast."
Kren A., Mamnun Y.M., Bauer B.E., Schueller C., Wolfger H., Hatzixanthis K., Mollapour M., Gregori C., Piper P.W., Kuchler K.
Mol. Cell. Biol. 23:1775-1785(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, PHOSPHORYLATION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae."
Schueller C., Mamnun Y.M., Mollapour M., Krapf G., Schuster M., Bauer B.E., Piper P.W., Kuchler K.
Mol. Biol. Cell 15:706-720(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"A new physiological role for Pdr12p in Saccharomyces cerevisiae: export of aromatic and branched-chain organic acids produced in amino acid catabolism."
Hazelwood L.A., Tai S.L., Boer V.M., de Winde J.H., Pronk J.T., Daran J.-M.
FEMS Yeast Res. 6:937-945(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"A genetic screen identifies mutations in the yeast WAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation."
Gregori C., Bauer B.E., Schwartz C., Kren A., Schueller C., Kuchler K.
FEBS J. 274:3094-3107(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-762; PHE-763 AND ARG-764.
[9]"Weak organic acids trigger conformational changes of the yeast transcription factor War1 in vivo to elicit stress adaptation."
Gregori C., Schueller C., Frohner I.E., Ammerer G., Kuchler K.
J. Biol. Chem. 283:25752-25764(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF SER-368; TYR-452; TYR-463; ALA-640; SER-703 AND LYS-762.
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-128, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46373 Genomic DNA. Translation: CAA86502.1.
BK006946 Genomic DNA. Translation: DAA09821.1.
PIRS48821.
RefSeqNP_013635.1. NM_001182435.1.

3D structure databases

ProteinModelPortalQ03631.
SMRQ03631. Positions 74-116.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35065. 16 interactions.
MINTMINT-2734616.
STRING4932.YML076C.

Proteomic databases

MaxQBQ03631.
PaxDbQ03631.
PeptideAtlasQ03631.
PRIDEQ03631.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML076C; YML076C; YML076C.
GeneID854899.
KEGGsce:YML076C.

Organism-specific databases

CYGDYML076c.
SGDS000004541. WAR1.

Phylogenomic databases

eggNOGNOG46074.
HOGENOMHOG000142350.
OMANQTRKRR.
OrthoDBEOG7Q2NDP.

Enzyme and pathway databases

BioCycYEAST:G3O-32668-MONOMER.

Gene expression databases

GenevestigatorQ03631.

Family and domain databases

InterProIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
SMARTSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMSSF57701. SSF57701. 1 hit.
PROSITEPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977873.

Entry information

Entry nameWAR1_YEAST
AccessionPrimary (citable) accession number: Q03631
Secondary accession number(s): D6W0K7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families