Q03631 (WAR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Weak acid resistance protein 1

Gene names

Name:	WAR1
Ordered Locus Names:	YML076C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	944 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	transcription factor which binds to a weak acid response element (WARE) to mediate stress induction of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively. Ref.3 Ref.6 Ref.7 Ref.8 Ref.9
Subunit structure	Homodimer. Ref.3
Subcellular location	Nucleus Ref.3 Ref.4 Ref.8.
Post-translational modification	Phosphorylation is required for PDR12 induction.
Miscellaneous	Present with 907 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to acid Inferred from mutant phenotype Ref.3. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD nucleus Inferred from direct assay Ref.3. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype Ref.3. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 944	944	Weak acid resistance protein 1		PRO_0000115004
Regions
	DNA binding	76 – 109	34	Zn(2)-C6 fungal-type
Amino acid modifications
	Modified residue	124	1	Phosphoserine Ref.10
Experimental info
	Mutagenesis	368	1	S → L: Causes hyperactivity with constitutive induction of PDR12. Ref.9
	Mutagenesis	452	1	Y → C: Causes hyperactivity with constitutive induction of PDR12. Ref.9
	Mutagenesis	463	1	Y → C: Causes hyperactivity with constitutive induction of PDR12. Ref.9
	Mutagenesis	640	1	A → T: Causes hyperactivity with constitutive induction of PDR12. Ref.9
	Mutagenesis	703	1	S → P: Causes hyperactivitywith constitutive induction of PDR12. Ref.9
	Mutagenesis	762	1	K → R or N: Causes hyperactivity with constitutive induction of PDR12. Ref.8 Ref.9
	Mutagenesis	763	1	F → M: Causes hyperactivity with constitutive induction of PDR12. Ref.8
	Mutagenesis	764	1	Missing: Causes hypersensitivity to sorbate through its inability to induce PDR12. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q03631 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 020A56745DF52CCC	FASTA	944	107,561
	10 20 30 40 50 60 MDTQIAITGV AVGKEINNDN SKTDQKVSLP KADVPCIDKA TQTIIEGCSK DDPRLSYPTK 70 80 90 100 110 120 LETTEKGKTK RNSFACVCCH SLKQKCEPSD VNDIYRKPCR RCLKHKKLCK FDLSKRTRKR 130 140 150 160 170 180 KPRSRSPTPF ESPMVNVSTK SKGPTDSEES SLKDGTSYLA SFPSDPNAKQ FPNSRTVLPG 190 200 210 220 230 240 LQQSLSDLWS TLSQPPSYGA REAETTSTGE ITTNNHTKSN GSVPTNPAVL ASNDEHTNIS 250 260 270 280 290 300 DAPVIYSTYN SPVPISSAPT SINSEALFKH RPKIVGDEET QNVKVKRQKK SYSRHMTRSF 310 320 330 340 350 360 RKQLQSLIIS QKGKIRDISM KLDTWSKQWN DLVEKSMFLP TIADPVSVGI ISHEEATLRL 370 380 390 400 410 420 HLYKTEISYL SKLPFIKVEE NVSVDELRKK KPILFSVIMS CVSIVLTPKQ TTRGTIMKLD 430 440 450 460 470 480 SFVLNLITNQ IFKANNKSIE IIESLSTLCL WYNFFEWSSK TRYHIFNYIC CCLTRDLGPT 490 500 510 520 530 540 YVNRSFGMFS DEDPKRFKSP LELYSNGASL TLLVYISALN ISIFLRQSIQ ARWSHVTEKA 550 560 570 580 590 600 CEDLVKETKK SRHYDNDKLL LDSADDPILV QFAKMNHVLE NIHTHLHERD LNDDEFDDPI 610 620 630 640 650 660 FTKKYLNKLM EKYHKQLQEI FTKLDRNRPR VIAFYYSVEA YLYQYKLAVF IGEMSHTINE 670 680 690 700 710 720 KVELPREIMD DFVKCYHCCK SALEEFSKLE PILITSLPLF HTSRIIYTVG MLLLKLRYSV 730 740 750 760 770 780 VAIPSFHDLM PLTDDAIALV IGVNNLLEKT SELYPFNNSL YKFRYVIALF CQTYANKVID 790 800 810 820 830 840 VADRYNAERE KLKEKQVIDE VSNGHDGTKP INAYVTESQK MPTEEDPIID NNTNQNITAV 850 860 870 880 890 900 PDEMLPVYSR VRDDTAAMNL NINSTSYMNE SPHEHRESMT GTTLLPPPFI SNDVTNSADS 910 920 930 940 TNIKPSPSSS VDNLNDYLTD INSLAWGVNS LNDEFWTDLF MNDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"War1p, a novel transcription factor controlling weak acid stress response in yeast." Kren A., Mamnun Y.M., Bauer B.E., Schueller C., Wolfger H., Hatzixanthis K., Mollapour M., Gregori C., Piper P.W., Kuchler K. Mol. Cell. Biol. 23:1775-1785(2003) [PubMed: 12588995] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, PHOSPHORYLATION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae." Schueller C., Mamnun Y.M., Mollapour M., Krapf G., Schuster M., Bauer B.E., Piper P.W., Kuchler K. Mol. Biol. Cell 15:706-720(2004) [PubMed: 14617816] [Abstract] Cited for: FUNCTION.
[7]	"A new physiological role for Pdr12p in Saccharomyces cerevisiae: export of aromatic and branched-chain organic acids produced in amino acid catabolism." Hazelwood L.A., Tai S.L., Boer V.M., de Winde J.H., Pronk J.T., Daran J.-M. FEMS Yeast Res. 6:937-945(2006) [PubMed: 16911515] [Abstract] Cited for: FUNCTION.
[8]	"A genetic screen identifies mutations in the yeast WAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation." Gregori C., Bauer B.E., Schwartz C., Kren A., Schueller C., Kuchler K. FEBS J. 274:3094-3107(2007) [PubMed: 17509074] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-762; PHE-763 AND ARG-764.
[9]	"Weak organic acids trigger conformational changes of the yeast transcription factor War1 in vivo to elicit stress adaptation." Gregori C., Schueller C., Frohner I.E., Ammerer G., Kuchler K. J. Biol. Chem. 283:25752-25764(2008) [PubMed: 18621731] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF SER-368; TYR-452; TYR-463; ALA-640; SER-703 AND LYS-762.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46373 Genomic DNA. Translation: CAA86502.1. BK006946 Genomic DNA. Translation: DAA09821.1.
PIR	S48821.
RefSeq	NP_013635.1. NM_001182435.1.
3D structure databases
ProteinModelPortal	Q03631.
SMR	Q03631. Positions 71-143.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03631. 1 interaction.
MINT	MINT-2734616.
STRING	Q03631.
Proteomic databases
PeptideAtlas	Q03631.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML076C; YML076C; YML076C.
GeneID	854899.
KEGG	sce:YML076C.
NMPDR	fig|4932.3.peg.4672.
Organism-specific databases
CYGD	YML076c.
SGD	S000004541. WAR1.
Phylogenomic databases
eggNOG	fuNOG04302.
HOGENOM	HBG203538.
OMA	NQTRKRR.
OrthoDB	EOG4GJ2Z0.
Gene expression databases
ArrayExpress	Q03631.
Genevestigator	Q03631.
GermOnline	YML076C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.240.10. Zn2-C6_fun-type_DNA-bd. 1 hit.
SMART	SM00066. GAL4. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	977873.

Entry information

Entry name

WAR1_YEAST

Accession

Primary (citable) accession number: Q03631
Secondary accession number(s): D6W0K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program