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Q03630 (BET5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trafficking protein particle complex subunit BET5

Short name=TRAPP subunit BET5
Alternative name(s):
Transport protein particle 18 kDa subunit
Gene names
Name:BET5
Ordered Locus Names:YML077W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication. Ref.4 Ref.6 Ref.7 Ref.8

Subunit structure

Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Golgi apparatuscis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure Ref.6 Ref.8.

Sequence similarities

Belongs to the TRAPP small subunits family. BET5 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BET3P3614910EBI-3580,EBI-3567

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 159159Trafficking protein particle complex subunit BET5
PRO_0000211565

Secondary structure

....................... 159
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q03630 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 4821217270F516E9

FASTA15918,434
        10         20         30         40         50         60 
MGIYSFWIFD RHCNCIFDRE WTLASNSASG TINSKQNEED AKLLYGMIFS LRSITQKLSK 

        70         80         90        100        110        120 
GSVKNDIRSI STGKYRVHTY CTASGLWFVL LSDFKQQSYT QVLQYIYSHI YVKYVSNNLL 

       130        140        150 
SPYDFAENEN EMRGQGTRKI TNRNFISVLE SFLAPMVNQ 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"A high copy suppressor screen reveals genetic interactions between BET3 and a new gene. Evidence for a novel complex in ER-to-Golgi transport."
Jiang Y., Scarpa A., Zhang L., Stone S., Feliciano E., Ferro-Novick S.
Genetics 149:833-841(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
[6]"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
[7]"Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
Yip C.K., Berscheminski J., Walz T.
Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[8]"Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy."
Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R., Brumell J.H., Ferro-Novick S., Klionsky D.J.
Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46373 Genomic DNA. Translation: CAA86501.1.
AY557760 Genomic DNA. Translation: AAS56086.1.
BK006946 Genomic DNA. Translation: DAA09820.1.
PIRS48820.
RefSeqNP_013634.1. NM_001182436.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUEX-ray3.70C/I/O/U1-159[»]
ProteinModelPortalQ03630.
SMRQ03630. Positions 1-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35064. 40 interactions.
DIPDIP-4528N.
IntActQ03630. 5 interactions.
MINTMINT-478474.
STRING4932.YML077W.

Proteomic databases

MaxQBQ03630.
PaxDbQ03630.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML077W; YML077W; YML077W.
GeneID854898.
KEGGsce:YML077W.

Organism-specific databases

CYGDYML077w.
SGDS000004542. BET5.

Phylogenomic databases

eggNOGNOG237222.
GeneTreeENSGT00510000047863.
HOGENOMHOG000172118.
OMAFYETPAN.
OrthoDBEOG71CFZH.

Enzyme and pathway databases

BioCycYEAST:G3O-32669-MONOMER.

Gene expression databases

GenevestigatorQ03630.

Family and domain databases

InterProIPR011012. Longin-like_dom.
IPR007233. Sybindin.
[Graphical view]
PANTHERPTHR23249. PTHR23249. 1 hit.
PfamPF04099. Sybindin. 1 hit.
[Graphical view]
SUPFAMSSF64356. SSF64356. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ03630.
NextBio977870.
PROQ03630.

Entry information

Entry nameBET5_YEAST
AccessionPrimary (citable) accession number: Q03630
Secondary accession number(s): D6W0K6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references