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Q03603

- DGK3_CAEEL

UniProt

Q03603 - DGK3_CAEEL

Protein

Probable diacylglycerol kinase 3

Gene

dgk-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (16 May 2012)
      Previous versions | rss
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    Functioni

    Involved in AFD-neuron mediated thermotaxis. Regulates behavior to environmental temperature. Thought to have a role in olfactory adaptation by affecting diacylglycerol levels.2 Publications

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi183 – 194121CuratedAdd
    BLAST
    Calcium bindingi228 – 239122CuratedAdd
    BLAST
    Zinc fingeri265 – 31652Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri329 – 37547Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. diacylglycerol kinase activity Source: UniProtKB-EC
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. olfactory behavior Source: UniProtKB
    3. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
    4. regulation of long-term neuronal synaptic plasticity Source: WormBase
    5. thermotaxis Source: WormBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable diacylglycerol kinase 3 (EC:2.7.1.107)
    Short name:
    DAG kinase 3
    Alternative name(s):
    Diglyceride kinase 3
    Short name:
    DGK-3
    Gene namesi
    Name:dgk-3
    ORF Names:F54G8.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiF54G8.2; CE46230; WBGene00000960; dgk-3.

    Pathology & Biotechi

    Disruption phenotypei

    Slow to adapt to new temperature levels. Dgk-3 and dgk-1 double mutant shows defects in olfactory adaptation.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 795795Probable diacylglycerol kinase 3PRO_0000218471Add
    BLAST

    Proteomic databases

    PaxDbiQ03603.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi6239.F54G8.2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03603.
    SMRiQ03603. Positions 2-75, 159-243, 266-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini170 – 20536EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini215 – 25036EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini423 – 558136DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri265 – 31652Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri329 – 37547Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00750000117569.
    HOGENOMiHOG000252931.
    InParanoidiQ03603.
    OMAiCTVRITH.
    OrthoDBiEOG75XGK8.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.238.110. 2 hits.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03603-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLSPEQFSR LSEYAAYSRR KLKDMLSDFQ QDGKFYSYLS VDGQTINIDG    50
    FRAFLIDYFG ADLPSDLVDQ LFLSFSKPPI KERRTSLFED AISTVRAKFS 100
    ESLSGRMAGL NIAGGSGQQT DRSQSSEPQA LVCIPEDDVM GPRVANNDSQ 150
    EPRIPLKPLI CTLSLLEADT PENKLDVVFH VYDSDGNGFL DKSEIDGIIE 200
    QMMNVARYQQ WDTIELEQVI RQMMVDIDYD NDGIVSFDEW RRGGLTNIPL 250
    LVLLGFDTEM KEDGSHVWRL RHFTKPTYCN ACCSILVGWG GKQGLSCSLC 300
    KYTVHERCVR SAATNCIRTY SSRQQDKLYH HWQDANATAK CVKCKATVGV 350
    FQGKGCRWCH NYVHHRCMSA LAQECDLGAL VHHILPPTHI FPAFLERKTS 400
    TSLKNHNFSS HSASLLQAVS PSNDCRPLLV LVNPKSGGKQ GVKILQKFEY 450
    LLNPRQVYDL SKTGPEPGLQ LFSTLKNCNI LVCGGDGTIG WVLESMDKMT 500
    FPHGRPPVAV LPLGTGNDLA RCLRWGGGYE NENLHKILEQ IEKSSLIDMD 550
    RWQIKIEITE NKSARRASEK GDTPPYSIIN NYFSIGVDAS IAHRFHVMRE 600
    KFPEKFNSRM RNKLWYFELG TSETLSSSCK NLHEQIDILC DGESIDLGQD 650
    ASLEGIALLN IPSIYGGSNL WGRSRKSKGR MPGLFPMKNA EKMQLQTRVQ 700
    DIGDGLIELV GLESAMQMGQ IKAGVRGARR LSQCSTVVIQ THKSFPMQID 750
    GEPWMQPPCI IQITHKNQAK MLVAAAPRKR SSWMLLKRQS TNDDN 795
    Length:795
    Mass (Da):89,315
    Last modified:May 16, 2012 - v3
    Checksum:iA1427D2F9837616A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z19155 Genomic DNA. Translation: CAA79558.3.
    PIRiS28273.
    RefSeqiNP_499031.3. NM_066630.3.
    UniGeneiCel.10418.

    Genome annotation databases

    EnsemblMetazoaiF54G8.2; F54G8.2; WBGene00000960.
    GeneIDi186262.
    KEGGicel:CELE_F54G8.2.
    UCSCiF54G8.2. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z19155 Genomic DNA. Translation: CAA79558.3 .
    PIRi S28273.
    RefSeqi NP_499031.3. NM_066630.3.
    UniGenei Cel.10418.

    3D structure databases

    ProteinModelPortali Q03603.
    SMRi Q03603. Positions 2-75, 159-243, 266-324.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.F54G8.2.

    Proteomic databases

    PaxDbi Q03603.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F54G8.2 ; F54G8.2 ; WBGene00000960 .
    GeneIDi 186262.
    KEGGi cel:CELE_F54G8.2.
    UCSCi F54G8.2. c. elegans.

    Organism-specific databases

    CTDi 186262.
    WormBasei F54G8.2 ; CE46230 ; WBGene00000960 ; dgk-3.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00750000117569.
    HOGENOMi HOG000252931.
    InParanoidi Q03603.
    OMAi CTVRITH.
    OrthoDBi EOG75XGK8.

    Miscellaneous databases

    NextBioi 931226.
    PROi Q03603.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.238.110. 2 hits.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "A diacylglycerol kinase modulates long-term thermotactic behavioral plasticity in C. elegans."
      Biron D., Shibuya M., Gabel C., Wasserman S.M., Clark D.A., Brown A., Sengupta P., Samuel A.D.
      Nat. Neurosci. 9:1499-1505(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    4. "Goalpha regulates olfactory adaptation by antagonizing Gqalpha-DAG signaling in Caenorhabditis elegans."
      Matsuki M., Kunitomo H., Iino Y.
      Proc. Natl. Acad. Sci. U.S.A. 103:1112-1117(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiDGK3_CAEEL
    AccessioniPrimary (citable) accession number: Q03603
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: May 16, 2012
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3