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Protein

Probable diacylglycerol kinase 3

Gene

dgk-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in AFD-neuron mediated thermotaxis. Regulates behavior to environmental temperature. Thought to have a role in olfactory adaptation by affecting diacylglycerol levels.2 Publications

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi183 – 194121CuratedAdd
BLAST
Calcium bindingi228 – 239122CuratedAdd
BLAST
Zinc fingeri265 – 31652Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri329 – 37547Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • olfactory behavior Source: UniProtKB
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  • regulation of long-term neuronal synaptic plasticity Source: WormBase
  • thermotaxis Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_304295. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diacylglycerol kinase 3 (EC:2.7.1.107)
Short name:
DAG kinase 3
Alternative name(s):
Diglyceride kinase 3
Short name:
DGK-3
Gene namesi
Name:dgk-3
ORF Names:F54G8.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF54G8.2; CE46230; WBGene00000960; dgk-3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Slow to adapt to new temperature levels. Dgk-3 and dgk-1 double mutant shows defects in olfactory adaptation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 795795Probable diacylglycerol kinase 3PRO_0000218471Add
BLAST

Proteomic databases

PaxDbiQ03603.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi6239.F54G8.2.

Structurei

3D structure databases

ProteinModelPortaliQ03603.
SMRiQ03603. Positions 2-75, 159-243, 266-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini170 – 20536EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini215 – 25036EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini423 – 558136DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri265 – 31652Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri329 – 37547Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
InParanoidiQ03603.
KOiK00901.
OMAiQYSEYSS.
OrthoDBiEOG75XGK8.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 2 hits.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSPEQFSR LSEYAAYSRR KLKDMLSDFQ QDGKFYSYLS VDGQTINIDG
60 70 80 90 100
FRAFLIDYFG ADLPSDLVDQ LFLSFSKPPI KERRTSLFED AISTVRAKFS
110 120 130 140 150
ESLSGRMAGL NIAGGSGQQT DRSQSSEPQA LVCIPEDDVM GPRVANNDSQ
160 170 180 190 200
EPRIPLKPLI CTLSLLEADT PENKLDVVFH VYDSDGNGFL DKSEIDGIIE
210 220 230 240 250
QMMNVARYQQ WDTIELEQVI RQMMVDIDYD NDGIVSFDEW RRGGLTNIPL
260 270 280 290 300
LVLLGFDTEM KEDGSHVWRL RHFTKPTYCN ACCSILVGWG GKQGLSCSLC
310 320 330 340 350
KYTVHERCVR SAATNCIRTY SSRQQDKLYH HWQDANATAK CVKCKATVGV
360 370 380 390 400
FQGKGCRWCH NYVHHRCMSA LAQECDLGAL VHHILPPTHI FPAFLERKTS
410 420 430 440 450
TSLKNHNFSS HSASLLQAVS PSNDCRPLLV LVNPKSGGKQ GVKILQKFEY
460 470 480 490 500
LLNPRQVYDL SKTGPEPGLQ LFSTLKNCNI LVCGGDGTIG WVLESMDKMT
510 520 530 540 550
FPHGRPPVAV LPLGTGNDLA RCLRWGGGYE NENLHKILEQ IEKSSLIDMD
560 570 580 590 600
RWQIKIEITE NKSARRASEK GDTPPYSIIN NYFSIGVDAS IAHRFHVMRE
610 620 630 640 650
KFPEKFNSRM RNKLWYFELG TSETLSSSCK NLHEQIDILC DGESIDLGQD
660 670 680 690 700
ASLEGIALLN IPSIYGGSNL WGRSRKSKGR MPGLFPMKNA EKMQLQTRVQ
710 720 730 740 750
DIGDGLIELV GLESAMQMGQ IKAGVRGARR LSQCSTVVIQ THKSFPMQID
760 770 780 790
GEPWMQPPCI IQITHKNQAK MLVAAAPRKR SSWMLLKRQS TNDDN
Length:795
Mass (Da):89,315
Last modified:May 16, 2012 - v3
Checksum:iA1427D2F9837616A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19155 Genomic DNA. Translation: CAA79558.3.
PIRiS28273.
RefSeqiNP_499031.3. NM_066630.3.
UniGeneiCel.10418.

Genome annotation databases

EnsemblMetazoaiF54G8.2; F54G8.2; WBGene00000960.
GeneIDi186262.
KEGGicel:CELE_F54G8.2.
UCSCiF54G8.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19155 Genomic DNA. Translation: CAA79558.3.
PIRiS28273.
RefSeqiNP_499031.3. NM_066630.3.
UniGeneiCel.10418.

3D structure databases

ProteinModelPortaliQ03603.
SMRiQ03603. Positions 2-75, 159-243, 266-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F54G8.2.

Proteomic databases

PaxDbiQ03603.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF54G8.2; F54G8.2; WBGene00000960.
GeneIDi186262.
KEGGicel:CELE_F54G8.2.
UCSCiF54G8.2. c. elegans.

Organism-specific databases

CTDi186262.
WormBaseiF54G8.2; CE46230; WBGene00000960; dgk-3.

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
InParanoidiQ03603.
KOiK00901.
OMAiQYSEYSS.
OrthoDBiEOG75XGK8.

Enzyme and pathway databases

ReactomeiREACT_304295. Effects of PIP2 hydrolysis.

Miscellaneous databases

NextBioi931226.
PROiQ03603.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 2 hits.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "A diacylglycerol kinase modulates long-term thermotactic behavioral plasticity in C. elegans."
    Biron D., Shibuya M., Gabel C., Wasserman S.M., Clark D.A., Brown A., Sengupta P., Samuel A.D.
    Nat. Neurosci. 9:1499-1505(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. "Goalpha regulates olfactory adaptation by antagonizing Gqalpha-DAG signaling in Caenorhabditis elegans."
    Matsuki M., Kunitomo H., Iino Y.
    Proc. Natl. Acad. Sci. U.S.A. 103:1112-1117(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiDGK3_CAEEL
AccessioniPrimary (citable) accession number: Q03603
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 16, 2012
Last modified: June 24, 2015
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.