Reviewed, UniProtKB/Swiss-Prot Q03603 (DGK3_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable diacylglycerol kinase 3
      Short name=DAG kinase 3
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase 3
      Short name=DGK-3

Gene names

Name:	dgk-3
ORF Names:	F54G8.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	812 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 812	812	Probable diacylglycerol kinase 3		PRO_0000218471
Regions
	Domain	170 – 205	36	EF-hand 1
	Domain	215 – 250	36	EF-hand 2
	Domain	423 – 558	136	DAGKc
	Calcium binding	183 – 194	12	1 Probable
	Calcium binding	228 – 239	12	2 Probable
	Zinc finger	265 – 316	52	Phorbol-ester/DAG-type 1
	Zinc finger	329 – 375	47	Phorbol-ester/DAG-type 2

Sequences

	Sequence		Length	Mass (Da)
	Q03603-1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: E98832644C43BF85	FASTA	812	91,380
	10 20 30 40 50 60 MLLSPEQFSR LSEYAAYSRR KLKDMLSDFQ QDGKFYSYLS VDGQTINIDG FRAFLIDYFG 70 80 90 100 110 120 ADLPSDLVDQ LFLSFSKPPI KERRTSLFED AISTVRAKFS ESLSGRMAGL NIAGGSGQQT 130 140 150 160 170 180 DRSQSSEPQA LVCIPEDDVM GPRVANNDSQ EPRIPLKPLI CTLSLLEADT PENKLDVVFH 190 200 210 220 230 240 VYDSDGNGFL DKSEIDGIIE QMMNVARYQQ WDTIELEQVI RQMMVDIDYD NDGIVSFDEW 250 260 270 280 290 300 RRGGLTNIPL LVLLGFDTEM KEDGSHVWRL RHFTKPTYCN ACCSILVGWG GKQGLSCSLC 310 320 330 340 350 360 KYTVHERCVR SAATNCIRTY SSRQQDKLYH HWQDANATAK CVKCKATVGV FQGKGCRWCH 370 380 390 400 410 420 NYVHHRCMSA LAQECDLGAL VHHILPPTHI FPAFLERKTS TSLKNHNFSS HSASLLQAVS 430 440 450 460 470 480 PSNDCRPLLV LVNPKSGGKQ GVKILQKFEY LLNPRQVYDL SKTGPEPGLQ LFSTLKNCNI 490 500 510 520 530 540 LVCGGDGTIG WVLESMDKMT FPHGRPPVAV LPLGTGNDLA RCLRWGGGYE NENLHKILEQ 550 560 570 580 590 600 IEKSSLIDMD RWQIKIEITE NKSARRASEK GDTPPYSIIN NYFSIGVDAS IAHRFHVMRE 610 620 630 640 650 660 KFPEKFNSRM RNKLWYFELG TSETLSSSCK NLHEQIDILC DGESIDLGQD ASLEGIALLN 670 680 690 700 710 720 IPSIYGGSNL WGRSRKSKGR MPGLFPMKNA EKMQLQTRVQ DIGDGLIELV GLESAMQMGQ 730 740 750 760 770 780 IKAGVRGARR LSQCSTVVIQ THKSFPMQID GEPWMQPPCI IQITHKNQAK MLVGEFRHIL 790 800 810 ELSINFFLFT AAAPRKRSSW MLLKRQSTND DN

References

[1]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z19155 Genomic DNA. Translation: CAA79558.2.
PIR	S28273.
RefSeq	NP_499031.2.
UniGene	Cel.10418
3D structure databases
HSSP	HSSP built from PDB template 1TBN based on UniProtKB P05697.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03603.
Genome annotation databases
Ensembl	F54G8.2; F54G8.2; F54G8.2; Caenorhabditis elegans. [Genome view]
GeneID	186262.
KEGG	cel:F54G8.2.
NMPDR	fig|6239.3.peg.11080.
UCSC	F54G8.2. c. elegans.
Organism-specific databases
CTD	186262.
WormBase	WBGene00000960. dgk-3.
WormPep	F54G8.2. CE33654. [WorfDB]
Phylogenomic databases
OMA	IPSIYGG.
Enzyme and pathway databases
BRENDA	2.7.1.107. 672.
Gene expression databases
ArrayExpress	Q03603.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR018248. EF_hand. IPR018247. EF_HAND_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00036. efhand. 1 hit. [Graphical view]
ProDom	PD002939. DAGKa. 1 hit. PD005043. DAGKc. 1 hit. PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	931226.

Entry information

Entry name

DGK3_CAEEL

Accession

Primary (citable) accession number: Q03603

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	March 1, 2004
Last modified:	November 3, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project