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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Lactobacillus paracasei (strain ATCC 334 / BCRC 17002 / CIP 107868 / KCTC 3260 / NRRL B-441)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei262ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:LSEI_2313
OrganismiLactobacillus paracasei (strain ATCC 334 / BCRC 17002 / CIP 107868 / KCTC 3260 / NRRL B-441)
Taxonomic identifieri321967 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000001651 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000019111 – 497Glutamate--tRNA ligaseAdd BLAST497

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ035R5.
SMRiQ035R5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionUniRule annotationAdd BLAST11
Motifi259 – 263"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK09698.
OMAiAYYAFDT.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q035R5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRPIRVRY APSPTGHLHI GNARTALFNY LFARHNNGTL VLRIEDTDTK
60 70 80 90 100
RNVADGEKSQ MENLHWLGID WDEGPDKGGD FGPYRQSERK DIYDKLIKQL
110 120 130 140 150
VDKGFAYESY RTEEELKADR EAQKARHEMP HYEYEYEGMT DAEKADAIAA
160 170 180 190 200
AKAKGLEPVI RFHIPMDKTY EWDDIVKGKI SIDANTLGGD FVIQKRDGMP
210 220 230 240 250
TYNFAVVVDD HMMQISHVLR GDDHIANTPK QLAIYDAFGW EPPIFGHMTL
260 270 280 290 300
IINGATGKKL SKRDETVLQF IEQYRELGYL PDAMFNFITL LGWSPVGEDE
310 320 330 340 350
IFTKKEFIKQ FDPKRLSKSP ARFDQKKLEW VNNQYIKAKQ KTNPNELMSL
360 370 380 390 400
SLANLIEDGK IHSDPDPKTI EWARQLISLY TDQMSYTAQI SKLADIFFDK
410 420 430 440 450
ATDLTDDERK ELADDNAKPV IEAFAKKIRA LDTFDAYSIG GIVNEVKQET
460 470 480 490
KVKGRKLYMP IRIAATLRMH GPQLAETIEL MGRDQVLKNV DLVTGQL
Length:497
Mass (Da):56,871
Last modified:November 14, 2006 - v1
Checksum:i569919CD5CBEF5ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000423 Genomic DNA. Translation: ABJ71057.1.
RefSeqiWP_003576207.1. NC_008526.1.
YP_807499.1. NC_008526.1.

Genome annotation databases

EnsemblBacteriaiABJ71057; ABJ71057; LSEI_2313.
GeneIDi31582965.
4420877.
KEGGilca:LSEI_2313.
PATRICifig|321967.11.peg.2274.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSYE_LACP3
AccessioniPrimary (citable) accession number: Q035R5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 14, 2006
Last modified: June 7, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families