Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fumarate hydratase class II

Gene

fumC

Organism
Lactobacillus paracasei (strain ATCC 334 / BCRC 17002 / CIP 107868 / KCTC 3260 / NRRL B-441)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei183SubstrateUniRule annotation1
Active sitei184Proton donor/acceptorUniRule annotation1
Active sitei314UniRule annotation1
Binding sitei315SubstrateUniRule annotation1
Sitei327Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:LSEI_2410Imported
OrganismiLactobacillus paracasei (strain ATCC 334 / BCRC 17002 / CIP 107868 / KCTC 3260 / NRRL B-441)Imported
Taxonomic identifieri321967 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000001651 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ035H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 338Lyase_1InterPro annotationAdd BLAST326
Domaini404 – 457FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 102Substrate bindingUniRule annotation3
Regioni136 – 138Substrate bindingUniRule annotation3
Regioni320 – 322Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q035H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKYRTETDT LGPVEIPANA LWGPQTERSR HNFQAGPLML MPVIRALLQL
60 70 80 90 100
KKAAARANVA VDVLPAPKGQ LITRAVDALL ALPDEKLQKD FPLHVYQTGS
110 120 130 140 150
GTQTNMNVNE VISHQAAKLN PDLPILPNDD VNHSQSSNDT FPTVMNITAA
160 170 180 190 200
IAVNQLLHAV NQLCSVLYSK AVTYARVVKI GRTHLQDATP LTFGQEISGW
210 220 230 240 250
VAMMEHDQIA LKQLQPNLFY LAIGGTAVGT GLNADPNFGK IVAGRLSDVY
260 270 280 290 300
QLPLSAKTNK FAALAAHSEI SVVHGAIKTL AADLLKIAND IRFLASGPRA
310 320 330 340 350
GYGELRIPAN EPGSSIMPGK VNPTQAEALT MAAVRVMGND TTITVAASQG
360 370 380 390 400
NFELNVYKPV IISTFLESAD LLTKTVTSFT EKLAAGLTIH APHMRALVDH
410 420 430 440 450
SLMLVTALAP HIGYEKSAAI AQLAEQENLD LKAAAVKSGD VTAAQFDKWV

DPLKMTKA
Length:458
Mass (Da):49,061
Last modified:November 14, 2006 - v1
Checksum:i508D29E037996B0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000423 Genomic DNA. Translation: ABJ71148.1.
RefSeqiWP_011674886.1. NC_008526.1.
YP_807590.1. NC_008526.1.

Genome annotation databases

EnsemblBacteriaiABJ71148; ABJ71148; LSEI_2410.
GeneIDi4420990.
KEGGilca:LSEI_2410.
PATRICifig|321967.11.peg.2367.

Similar proteinsi

Entry informationi

Entry nameiQ035H4_LACP3
AccessioniPrimary (citable) accession number: Q035H4
Entry historyiIntegrated into UniProtKB/TrEMBL: November 14, 2006
Last sequence update: November 14, 2006
Last modified: September 27, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported