Q03586 (RPOA2_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit A''
EC=2.7.7.6

Gene names

Name:	rpoA2
Ordered Locus Names:	Ta0392

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00411
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00411
Sequence similarities	Belongs to the RNA polymerase beta' chain family.
Sequence caution	The sequence CAC11536.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	DNA-directed RNA polymerase subunit A'' HAMAP MF_00411		PRO_0000074028
Regions
	Region	1 – 123	123	Unknown HAMAP MF_00411
	Region	124 – 508	385	DNA-directed RNA polymerase subunit A" HAMAP MF_00411

Sequences

	Sequence		Length	Mass (Da)
	Q03586 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 1599FAF94897CA4C	FASTA	508	56,599
	10 20 30 40 50 60 MASLLWRDTS KNIAAILEKL PADYAVDYDV PNNVEDGYIT INKKNFTYHV VISGVRKYSP 70 80 90 100 110 120 DVEAIVKKKS GLKSIITIEK VEKIEPLSFM EFRVGGKTLE AMGSFEVAER QVTEIKEKYG 130 140 150 160 170 180 ENLSEDVQKV LDDARAMGFT LPESVAEEIA RRRTEWGEKA YKNILKRIGE EIGNELIDPY 190 200 210 220 230 240 EAVGIIAAQS IGEPGTQMTM RTFHFAGVRE MNVTLGLPRL IEIVDARRIP STPSMTIYLR 250 260 270 280 290 300 PEYETNDEVV MDVVKRLENT SISDVADIIT DIGELTVTVR PDPRKTKDRL IEMEDIMNAI 310 320 330 340 350 360 SKIKGITVME DSGQIIIKPQ QESFKKLYLL QEQIKGLTIK GISGIKRAIA RVEGKEHRWV 370 380 390 400 410 420 IYTQGSNLKD VLEVDEVDPT RTYTNDIVEI ANVLGIEAAR NAILNEALRT LQEQGLNVDV 430 440 450 460 470 480 RHLMLVADMM TFSGSVRAVG RTGISGRKSS VLARAAFEIT TKHLLRAGIM GEVDKLAGVA 490 500 ENIIVGQPIT LGTGAVDIIY KGYPKTKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the genes encoding the subunits H, B, A' and A'' of the DNA-dependent RNA polymerase and the initiator tRNA from Thermoplasma acidophilum." Klenk H.-P., Renner O., Schwass V., Zillig W. Nucleic Acids Res. 20:5226-5226(1992) [PubMed: 1408839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
[2]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68198 Genomic DNA. Translation: CAA48282.1. AL445064 Genomic DNA. Translation: CAC11536.1. Different initiation.
PIR	S26724.
RefSeq	NP_393872.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q03586.
SMR	Q03586. Positions 181-228.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1456008.
GenomeReviews	Gene locus Ta0392 in contig AL139299_GR.
KEGG	tac:Ta0392.
NMPDR	fig|273075.1.peg.394.
Phylogenomic databases
HOGENOM	HBG297327.
OMA	RPIGRHG.
ProtClustDB	PRK14897.
Enzyme and pathway databases
BioCyc	TACI273075:TA0392-MONOMER.
Family and domain databases
HAMAP	MF_00411. RNApol_arch_A. Fused. [Tree]
InterPro	IPR012757. DNA-dir_RpoA2. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03042.
Pfam	PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
TIGRFAMs	TIGR02389. RNA_pol_rpoA2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOA2_THEAC

Accession

Primary (citable) accession number: Q03586

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 16, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program