Q03585 (RPOA1_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit A'
EC=2.7.7.6

Gene names

Name:	rpoA1
Ordered Locus Names:	Ta0391

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Zinc.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	DNA-directed RNA polymerase subunit A'		PRO_0000074009
Regions
	Zinc finger	60 – 103	44	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03585 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 390C4554F02947D7	FASTA	885	100,075
	10 20 30 40 50 60 MMGISKRISS IKFALLSPDE IRKLSQVKVI TADTYDDDGY PIEHGLMDLH MGVIEPGLRC 70 80 90 100 110 120 ATCGGKVDEC PGHFGHIELA MPVVHVGFVK EIKMFLDATC RSCGRIKLTD DEIRTYLPEI 130 140 150 160 170 180 QKMDFETGDP EDIEILTKKY VDLASQRMVC PHCGAQQSKI ILDKPTTFRE EGTNVKITPK 190 200 210 220 230 240 EIRERLERIP DDDLIFFGFN PKTARPEWMV LTVLPVPPIN VRPSITLETG ERSEDDLTHK 250 260 270 280 290 300 LVDIIRISQR LRESRDNGSP QLIIEDLWDL LQFHVTTYFD NQTPGIPPAR HRSGRALKTL 310 320 330 340 350 360 VQRLKGKEGR FRSNLSGKRV SFSSRTVISP EPYLSVNEVG VPERAARELT VPVIVNQFNI 370 380 390 400 410 420 DEMRELIKRG RNPRDQFGRY VTGVNYVIRP DGRRIKITDQ NAAENADRID IGWTVERQLM 430 440 450 460 470 480 EGDIVLFNRQ PSLHRMSMMG HTVRILPGQT FRFNLAVCTP YNADFDGDEM NLHVIQKEEA 490 500 510 520 530 540 RAEARIIMKV QEQIMSPRFG GPIIGGIHDH VTALFLLTHN NPRYTHEEMV HIMAYLEPDL 550 560 570 580 590 600 LPEARIENGE KYYYGRDIFS TILPKGLNVR FRSKLCSGSS ERCEFEDDPS DTYVEIVDGK 610 620 630 640 650 660 MIHGTIDEAA VSPFSGAIID KIFRKFGSQE AARFIDRMTR LAVGFITYRG FSTGISDYDI 670 680 690 700 710 720 PESAVARIEE LVAQAEDRIN KLIETFRRGE LQPAPGRSVE DTLEMEILSE AGVVRDESGK 730 740 750 760 770 780 IASSYLGLKV PSVIMARSGA RATMLNISEV AGIVGQQSVR GGRLNRGYYN RTLPHFKRGD 790 800 810 820 830 840 IGADARGFVR SSYMTGLSPT EYFFHSIGGR EGLVDTAVRT SRSGYMQRRL INAFEDLKVD 850 860 870 880 DSREVKDTVG SLIQIRYGED GIDPTRSARG KAVDMNYILF DEERR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the genes encoding the subunits H, B, A' and A'' of the DNA-dependent RNA polymerase and the initiator tRNA from Thermoplasma acidophilum." Klenk H.-P., Renner O., Schwass V., Zillig W. Nucleic Acids Res. 20:5226-5226(1992) [PubMed: 1408839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
[2]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68198 Genomic DNA. Translation: CAA48281.1. AL445064 Genomic DNA. Translation: CAC11535.1.
PIR	S26723.
RefSeq	NP_393871.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q03585.
ModBase	Search...
Proteomic databases
PRIDE	Q03585.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1456007.
GenomeReviews	Gene locus Ta0391 in contig AL139299_GR.
KEGG	tac:Ta0391.
NMPDR	fig|273075.1.peg.393.
Phylogenomic databases
HOGENOM	HBG499785.
OMA	HKLVDII.
PhylomeDB	Q03585.
ProtClustDB	PRK08566.
Enzyme and pathway databases
BioCyc	TACI273075:TA0391-MONOMER.
Family and domain databases
InterPro	IPR012758. DNA-dir_RpoA1. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03041.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02390. RNA_pol_rpoA1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOA1_THEAC

Accession

Primary (citable) accession number: Q03585

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program