Q03579 (LIP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ceramide synthase subunit LIP1

Alternative name(s):
LAG1/LAC1-interacting protein 1

Gene names

Name:	LIP1
Ordered Locus Names:	YMR298W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ceramide synthase complex required for synthesis of ceramides. Ref.3
Subunit structure	Component of the ceramide synthase complex composed of at least LAC1, LAG1 and LIP1.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein Ref.3.
Sequence similarities	Belongs to the LIP1 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ceramide biosynthetic process Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay Ref.3. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay PubMed 14690591. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LAC1	P28496	6	EBI-27640,EBI-26585
LAG1	P38703	4	EBI-27640,EBI-10035

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 150	150	Ceramide synthase subunit LIP1		PRO_0000203352
Regions
	Topological domain	1 – 20	20	Cytoplasmic Ref.3
	Transmembrane	21 – 40	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	41 – 150	110	Lumenal Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q03579 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 75DBA35225C3065C	FASTA	150	17,207
	10 20 30 40 50 60 MSQPTPIITT KSAAKPKPKI FNLFRVCFIS LLLIAAVEYF KYGTRINYEW FHCTPIKEPQ 70 80 90 100 110 120 SGSVIKLWAR GGPSCDKRGE YKTIVKRITR DYEPNDEHLS FCIIENDNVP PVHYPIHEDK 130 140 150 GEPGYVAYVG YDTDSELVQE LCADSTIYHM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Lip1p: a novel subunit of acyl-CoA ceramide synthase." Vallee B., Riezman H. EMBO J. 24:730-741(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH LAC1 AND LAG1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80836 Genomic DNA. Translation: CAA56807.1. BK006946 Genomic DNA. Translation: DAA10199.1.
PIR	S47459.
RefSeq	NP_014027.1. NM_001182807.1.
3D structure databases
ProteinModelPortal	Q03579.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4392N.
IntAct	Q03579. 3 interactions.
MINT	MINT-506475.
STRING	4932.YMR298W.
Proteomic databases
PaxDb	Q03579.
PeptideAtlas	Q03579.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR298W; YMR298W; YMR298W.
GeneID	855344.
KEGG	sce:YMR298W.
Organism-specific databases
CYGD	YMR298w.
SGD	S000004913. LIP1.
Phylogenomic databases
eggNOG	NOG42883.
HOGENOM	HOG000066058.
OMA	AVEYFKY.
OrthoDB	EOG49KK16.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-17699.
Gene expression databases
Genevestigator	Q03579.
GermOnline	YMR298W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	979088.

Entry information

Entry name

LIP1_YEAST

Accession

Primary (citable) accession number: Q03579
Secondary accession number(s): D6W0C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program