Reviewed, UniProtKB/Swiss-Prot Q03578 (DHE2_PORGI)

Names and origin

Protein names

Recommended name:
    NAD-specific glutamate dehydrogenase
      Short name=NAD-GDH
    EC=1.4.1.2
Alternative name(s):
    Surface-associated protein PGAG1

Gene names

Name:	gdh
Ordered Locus Names:	PG_1232

Organism

Porphyromonas gingivalis (Bacteroides gingivalis) [Complete proteome] [HAMAP]

Taxonomic identifier

837 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably involved in degradation rather than biosynthesis of glutamate.
Catalytic activity	L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH.
Subunit structure	Homohexamer Probable.
Subcellular location	Cell surface.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	NAD-specific glutamate dehydrogenase		PRO_0000182740
Sites
	Active site	124	1	By similarity
Experimental info
	Sequence conflict	258	1	S → Y in AAA50985. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q03578-1 [UniParc]. Last modified October 3, 2003. Version 2. Checksum: 7436A9C2E01C503D	FASTA	445	49,199
	10 20 30 40 50 60 MKTQEIMTML EAKHPGESEF LQAVKEVLLS VEEVYNQHPE FEKNGIIERI VEPDRVFTFR 70 80 90 100 110 120 VPWVDDQGKV QVNIGYRVQF NNAIGPYKGG IRFHPSVNLS ILKFLGFEQM FKNALTTLPM 130 140 150 160 170 180 GGGKGGADFS PKGKSEAEIM RFCQSFMTEL WRNIGPDTDI PAGDIGVGGR EVGYMFGMYK 190 200 210 220 230 240 KLAREHTGTL TGKGFEFGGS RLRPESTGFG AVYFVQNMCK QNGVDYKGKT LAISGFGNVA 250 260 270 280 290 300 WGVAQKATEL GIKVVTISGP DGYVYDPDGI NTPEKFRCML DLRDSGNDVV SDYVKRFPNA 310 320 330 340 350 360 QFFPGKKPWE QKVDFAMPCA TQNEMNLEDA KTLHKNGVTL VAETSNMGCT AEASEYYVAN 370 380 390 400 410 420 KMLFAPGKAV NAGGVSCSGL EMTQNAMHLV WTNEEVDKWL HQIMQDIHEQ CVTYGKDGNY 430 440 IDYVKGANIA GFMKVAKAMV AQGVC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a Porphyromonas gingivalis gene encoding a surface-associated glutamate dehydrogenase and construction of a glutamate dehydrogenase-deficient isogenic mutant." Joe A., Murray C.S., McBride B.C. Infect. Immun. 62:1358-1368(1994) [PubMed: 8132343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33277 / DSM 20709 / JCM 12257 / NCTC 11834 / 2561.
[2]	"Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83." Nelson K.E., Fleischmann R.D., DeBoy R.T., Paulsen I.T., Fouts D.E., Eisen J.A., Daugherty S.C., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Nelson W.C., Mason T.M., Tallon L., Gray J., Granger D., Tettelin H. , Dong H., Galvin J.L., Duncan M.J., Dewhirst F.E., Fraser C.M. J. Bacteriol. 185:5591-5601(2003) [PubMed: 12949112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-308 / W83.
[3]	"Characterization of recombinant and native forms of a cell surface antigen of Porphyromonas (Bacteroides) gingivalis." Joe A., Yamamoto A., McBride B.C. Infect. Immun. 61:3294-3303(1993) [PubMed: 8392971] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-29.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07290 Genomic DNA. Translation: AAA50985.1. AE015924 Genomic DNA. Translation: AAQ66318.1.
RefSeq	NP_905419.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Genome annotation databases
GeneID	2553197.
GenomeReviews	Gene locus PG_1232 in contig AE015924_GR.
KEGG	pgi:PG1232.
NMPDR	fig|242619.1.peg.1060.
TIGR	PG_1232.
Phylogenomic databases
HOGENOM	Q03578.
Enzyme and pathway databases
BioCyc	PGIN242619:PG1232-MON.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE2_PORGI

Accession

Primary (citable) accession number: Q03578

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 3, 2003
Last modified:	November 25, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)