Reviewed, UniProtKB/Swiss-Prot Q03577 (SYDC_CAEEL)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	drs-1
ORF Names:	B0464.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 531	531	Aspartyl-tRNA synthetase, cytoplasmic		PRO_0000111013

Sequences

	Sequence		Length	Mass (Da)
	Q03577-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 094E3207DC102733	FASTA	531	59,939
	10 20 30 40 50 60 MADAAEGEQP KLSKKELNKL ARKAKKDEKA GEKGGNQQQA AAMDQEDASK DFYGSYGLVN 70 80 90 100 110 120 SKEKKVLNFL KVKEINVSNA TKDVWVRGRI HTTRSKGKNC FLVLRQGVYT VQVAMFMNEK 130 140 150 160 170 180 ISKQMLKFVS SISKESIVDV YATINKVDNP IESCTQKDVE LLAQQVFVVS TSAPKLPLQI 190 200 210 220 230 240 EDASRRAPTD EEKASEQENQ LAVVNLDTRL DNRVIDLRTP TSHAIFRIQA GICNQFRNIL 250 260 270 280 290 300 DVRGFVEIMA PKIISAPSEG GANVFEVSYF KGSAYLAQSP QLYKQMAIAG DFEKVYTIGP 310 320 330 340 350 360 VFRAEDSNTH RHMTEFVGLD LEMAFNFHYH EVMETIAEVL TQMFKGLQQN YQDEIAAVGN 370 380 390 400 410 420 QYPAEPFQFC EPPLILKYPD AITLLRENGI EIGDEDDLST PVEKFLGKLV KEKYSTDFYV 430 440 450 460 470 480 LDKFPLSVRP FYTMPDAHDE RYSNSYDMFM RGEEILSGAQ RIHDADMLVE RAKHHQVDLA 490 500 510 520 530 KIQSYIDSFK YGCPPHAGGG IGLERVTMLF LGLHNIRLAS LFPRDPKRLT P

References

[1]

"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

[2]

"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z19152 Genomic DNA. Translation: CAA79536.1.
PIR	S28278.
RefSeq	NP_499089.1.
UniGene	Cel.7882
3D structure databases
HSSP	HSSP built from PDB template 1N9W based on UniProtKB Q9LCY8.
ModBase	Search...
Genome annotation databases
Ensembl	B0464.1. Caenorhabditis elegans. [Contig view]
GeneID	176334.
KEGG	cel:B0464.1.
Organism-specific databases
WormBase	WBGene00001094. drs-1.
WormPep	B0464.1. CE00015. [WorfDB]
Phylogenomic databases
OMA	Q03577. RNPKQSN.
Enzyme and pathway databases
BRENDA	6.1.1.12. 672.
Gene expression databases
ArrayExpress	Q03577.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR004523. Asp-tRNA-synth_IIb_arc/euk. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00458. aspS_arch. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	892140.

Entry information

Entry name

SYDC_CAEEL

Accession

Primary (citable) accession number: Q03577

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project