Q03559 (YM8V_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YMR295C

Gene names

Ordered Locus Names:

YMR295C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous	Present with 10800 molecules/cell in log phase SD medium.
Sequence similarities	To yeast YGR273c.

Ontologies

Keywords
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cellular bud Inferred from direct assay PubMed 14562095. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Uncharacterized protein YMR295C		PRO_0000203351
Amino acid modifications
	Modified residue	8	1	Phosphoserine Ref.8
	Modified residue	9	1	Phosphoserine Ref.8
	Modified residue	11	1	Phosphoserine Ref.4 Ref.8 Ref.10
	Modified residue	13	1	Phosphothreonine Ref.8 Ref.10
	Modified residue	14	1	Phosphoserine Ref.4 Ref.8 Ref.9 Ref.10
	Modified residue	23	1	Phosphoserine Ref.8
	Modified residue	74	1	Phosphoserine Ref.7 Ref.9
	Cross-link	26		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.6
	Cross-link	32		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q03559 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: BA9A4F868E9DCE8D	FASTA	197	22,179
	10 20 30 40 50 60 MMHFRKKSSI SNTSDHDGAN RASDVKISED DKARLKMRTA SVADPILDAV QEAQPFEQAA 70 80 90 100 110 120 DTFHDNMNRQ SYFSNEEGHV LCDVFGQPIT QADISNPTRA RDERPLDTIR SFEYAVSGDP 130 140 150 160 170 180 VWAQQLETPT YGFRVRPDFP VFGAAVTYDA NGMPQQVGGA SSQMYGEQAV YQPQQHVQTE 190 EKQKKKKKGL FGRMKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND SER-14, MASS SPECTROMETRY. Strain: 2124.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-26 AND LYS-32, MASS SPECTROMETRY. Strain: SUB592.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-9; SER-11; THR-13; SER-14 AND SER-23, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 AND SER-74, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11; THR-13 AND SER-14, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80836 Genomic DNA. Translation: CAA56804.1. AY557969 Genomic DNA. Translation: AAS56295.1. BK006946 Genomic DNA. Translation: DAA10196.1.
PIR	S47456.
RefSeq	NP_014024.1. NM_001182804.1.
3D structure databases
ProteinModelPortal	Q03559.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1843N.
IntAct	Q03559. 4 interactions.
MINT	MINT-402720.
STRING	4932.YMR295C.
Proteomic databases
PaxDb	Q03559.
PeptideAtlas	Q03559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR295C; YMR295C; YMR295C.
GeneID	855341.
KEGG	sce:YMR295C.
Organism-specific databases
CYGD	YMR295c.
SGD	S000004910. YMR295C.
Phylogenomic databases
eggNOG	NOG74880.
GeneTree	ENSGT00530000067701.
HOGENOM	HOG000246490.
OMA	WGFHEDF.
OrthoDB	EOG4H75N4.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32965-MONOMER.
Gene expression databases
Genevestigator	Q03559.
GermOnline	YMR295C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018809. DUF2406. [Graphical view]
Pfam	PF10295. DUF2406. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979079.

Entry information

Entry name

YM8V_YEAST

Accession

Primary (citable) accession number: Q03559
Secondary accession number(s): D6W0C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program