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Protein

NADPH dehydrogenase 2

Gene

OYE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381FMNBy similarity
Binding sitei192 – 1921FMNBy similarity
Binding sitei192 – 1921SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei197 – 1971Proton donorBy similarity
Binding sitei244 – 2441FMNBy similarity
Binding sitei349 – 3491FMNBy similarity
Binding sitei376 – 3761SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciYEAST:YHR179W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH dehydrogenase 2 (EC:1.6.99.1)
Alternative name(s):
Old yellow enzyme 2
Gene namesi
Name:OYE2
Ordered Locus Names:YHR179W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR179W.
SGDiS000001222. OYE2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 400399NADPH dehydrogenase 2PRO_0000194474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei353 – 3531PhosphoserineCombined sources
Modified residuei379 – 3791PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03558.
TopDownProteomicsiQ03558.

PTM databases

iPTMnetiQ03558.

Interactioni

Subunit structurei

Homodimer or heterodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
OYE3P418162EBI-12729,EBI-12734

Protein-protein interaction databases

BioGridi36612. 75 interactions.
DIPiDIP-213N.
IntActiQ03558. 9 interactions.
MINTiMINT-8285266.

Structurei

3D structure databases

ProteinModelPortaliQ03558.
SMRiQ03558. Positions 3-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000064642.
HOGENOMiHOG000116231.
InParanoidiQ03558.
KOiK00354.
OMAiWTKIFKA.
OrthoDBiEOG7M6DK6.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFVKDFKPQ ALGDTNLFKP IKIGNNELLH RAVIPPLTRM RAQHPGNIPN
60 70 80 90 100
RDWAVEYYAQ RAQRPGTLII TEGTFPSPQS GGYDNAPGIW SEEQIKEWTK
110 120 130 140 150
IFKAIHENKS FAWVQLWVLG WAAFPDTLAR DGLRYDSASD NVYMNAEQEE
160 170 180 190 200
KAKKANNPQH SITKDEIKQY VKEYVQAAKN SIAAGADGVE IHSANGYLLN
210 220 230 240 250
QFLDPHSNNR TDEYGGSIEN RARFTLEVVD AVVDAIGPEK VGLRLSPYGV
260 270 280 290 300
FNSMSGGAET GIVAQYAYVL GELERRAKAG KRLAFVHLVE PRVTNPFLTE
310 320 330 340 350
GEGEYNGGSN KFAYSIWKGP IIRAGNFALH PEVVREEVKD PRTLIGYGRF
360 370 380 390 400
FISNPDLVDR LEKGLPLNKY DRDTFYKMSA EGYIDYPTYE EALKLGWDKN
Length:400
Mass (Da):45,011
Last modified:January 23, 2007 - v3
Checksum:i67D4FF141B40324E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901W → R in AAS56612 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06124 Genomic DNA. Translation: AAA83386.1.
U00027 Genomic DNA. Translation: AAB68024.1.
AY558286 Genomic DNA. Translation: AAS56612.1.
BK006934 Genomic DNA. Translation: DAA06872.1.
PIRiA46009.
RefSeqiNP_012049.1. NM_001179310.1.

Genome annotation databases

EnsemblFungiiYHR179W; YHR179W; YHR179W.
GeneIDi856584.
KEGGisce:YHR179W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06124 Genomic DNA. Translation: AAA83386.1.
U00027 Genomic DNA. Translation: AAB68024.1.
AY558286 Genomic DNA. Translation: AAS56612.1.
BK006934 Genomic DNA. Translation: DAA06872.1.
PIRiA46009.
RefSeqiNP_012049.1. NM_001179310.1.

3D structure databases

ProteinModelPortaliQ03558.
SMRiQ03558. Positions 3-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36612. 75 interactions.
DIPiDIP-213N.
IntActiQ03558. 9 interactions.
MINTiMINT-8285266.

PTM databases

iPTMnetiQ03558.

Proteomic databases

MaxQBiQ03558.
TopDownProteomicsiQ03558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR179W; YHR179W; YHR179W.
GeneIDi856584.
KEGGisce:YHR179W.

Organism-specific databases

EuPathDBiFungiDB:YHR179W.
SGDiS000001222. OYE2.

Phylogenomic databases

GeneTreeiENSGT00530000064642.
HOGENOMiHOG000116231.
InParanoidiQ03558.
KOiK00354.
OMAiWTKIFKA.
OrthoDBiEOG7M6DK6.

Enzyme and pathway databases

BioCyciYEAST:YHR179W-MONOMER.

Miscellaneous databases

PROiQ03558.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Old Yellow Enzyme. The discovery of multiple isozymes and a family of related proteins."
    Stott K., Saito K., Thiels D.J., Massey V.
    J. Biol. Chem. 268:6097-6106(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF N-TERMINUS, CLEAVAGE OF INITIATOR METHIONINE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353 AND SER-379, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOYE2_YEAST
AccessioniPrimary (citable) accession number: Q03558
Secondary accession number(s): D3DLC8, E9P8V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.