Reviewed, UniProtKB/Swiss-Prot Q03558 (OYE2_YEAST)

Names and origin

Protein names

Recommended name:
    NADPH dehydrogenase 2
    EC=1.6.99.1
Alternative name(s):
    Old yellow enzyme 2

Gene names

Name:	OYE2
Ordered Locus Names:	YHR179W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.
Catalytic activity	NADPH + acceptor = NADP(+) + reduced acceptor.
Cofactor	FMN.
Subunit structure	Homodimer or heterodimer.
Miscellaneous	Present with 15100 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Ref.1 Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
OYE3	P41816	1	EBI-12729,EBI-12734
PRP40	P33203	1	EBI-12729,EBI-701
RSP5	P39940	1	EBI-12729,EBI-16219

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 400	399	NADPH dehydrogenase 2		PRO_0000194474
Sites
	Active site	197	1	Proton donor By similarity
	Binding site	38	1	FMN By similarity
	Binding site	192	1	FMN By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity
	Binding site	244	1	FMN By similarity
	Binding site	349	1	FMN By similarity
	Binding site	376	1	Substrate By similarity
Amino acid modifications
	Modified residue	137	1	Phosphoserine
	Modified residue	305	1	Phosphotyrosine
	Modified residue	353	1	Phosphoserine
	Modified residue	379	1	Phosphoserine
	Modified residue	388	1	Phosphothreonine

Sequences

	Sequence		Length	Mass (Da)
	Q03558-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 67D4FF141B40324E	FASTA	400	45,011
	10 20 30 40 50 60 MPFVKDFKPQ ALGDTNLFKP IKIGNNELLH RAVIPPLTRM RAQHPGNIPN RDWAVEYYAQ 70 80 90 100 110 120 RAQRPGTLII TEGTFPSPQS GGYDNAPGIW SEEQIKEWTK IFKAIHENKS FAWVQLWVLG 130 140 150 160 170 180 WAAFPDTLAR DGLRYDSASD NVYMNAEQEE KAKKANNPQH SITKDEIKQY VKEYVQAAKN 190 200 210 220 230 240 SIAAGADGVE IHSANGYLLN QFLDPHSNNR TDEYGGSIEN RARFTLEVVD AVVDAIGPEK 250 260 270 280 290 300 VGLRLSPYGV FNSMSGGAET GIVAQYAYVL GELERRAKAG KRLAFVHLVE PRVTNPFLTE 310 320 330 340 350 360 GEGEYNGGSN KFAYSIWKGP IIRAGNFALH PEVVREEVKD PRTLIGYGRF FISNPDLVDR 370 380 390 400 LEKGLPLNKY DRDTFYKMSA EGYIDYPTYE EALKLGWDKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Old Yellow Enzyme. The discovery of multiple isozymes and a family of related proteins." Stott K., Saito K., Thiels D.J., Massey V. J. Biol. Chem. 268:6097-6106(1993) [PubMed: 8454584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, MASS SPECTROMETRY.
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137; TYR-305; SER-353; SER-379 AND THR-388, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L06124 Genomic DNA. Translation: AAA83386.1. U00027 Genomic DNA. Translation: AAB68024.1.
PIR	A46009.
RefSeq	NP_012049.1.
3D structure databases
HSSP	HSSP built from PDB template 1OYC based on UniProtKB Q02899.
SMR	Q03558. Positions 3-399.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:213N.
IntAct	Q03558.
Proteomic databases
PeptideAtlas	Q03558.
Genome annotation databases
Ensembl	YHR179W. Saccharomyces cerevisiae. [Contig view]
GeneID	856584.
GenomeReviews	Gene locus YHR179W in contig U00093_GR.
KEGG	sce:YHR179W.
NMPDR	fig|4932.3.peg.3215.
Organism-specific databases
CYGD	YHR179w.
SGD	S000001222. OYE2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q03558.
Gene expression databases
GermOnline	YHR179W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q03558.
NextBio	982453.

Entry information

Entry name

OYE2_YEAST

Accession

Primary (citable) accession number: Q03558

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)