Q03555 (GEPH_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gephyrin

Alternative name(s):
Putative glycine receptor-tubulin linker protein

Including the following 2 domains:

1. Molybdopterin adenylyltransferase
   Short name=MPT adenylyltransferase
   EC=2.7.7.75
   Alternative name(s):
   Domain G
2. Molybdopterin molybdenumtransferase
   Short name=MPT Mo-transferase
   EC=2.10.1.1
   Alternative name(s):
   Domain E

Gene names

Name:	Gphn
Synonyms:	Gph

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	768 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules By similarity. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. Ref.5 Ref.7
Catalytic activity	ATP + molybdopterin = diphosphate + adenylyl-molybdopterin. Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP.
Cofactor	Magnesium.
Enzyme regulation	Inhibited by copper and tungsten By similarity.
Pathway	Cofactor biosynthesis; molybdopterin biosynthesis.
Subunit structure	Homotrimer, homodimer and homooligomer. Interacts with SRGAP2 (via SH3 domain) By similarity. Interacts with GLRB and GABARAP. Ref.4 Ref.6 Ref.8
Subcellular location	Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm › cytoskeleton. Note: Cytoplasmic face of glycinergic postsynaptic membranes. Ref.9
Tissue specificity	Expressed in tissues including spinal cord, brain, liver, kidney and lung.
Post-translational modification	Phosphorylated.
Sequence similarities	In the N-terminal section; belongs to the MoaB/Mog family. In the C-terminal section; belongs to the MoeA family.

Ontologies

Keywords
Biological process	Molybdenum cofactor biosynthesis
Cellular component	Cell junction Cell membrane Cytoplasm Cytoskeleton Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Molybdenum Nucleotide-binding
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW protein homooligomerization Inferred from direct assay PubMed 14976213. Source: RGD protein targeting Traceable author statement PubMed 11095995. Source: RGD synapse assembly Inferred from expression pattern PubMed 9130666. Source: RGD
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from direct assay PubMed 12967995. Source: RGD cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell inhibitory synapse Inferred from direct assay PubMed 18550748. Source: BHF-UCL plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding, bridging Traceable author statement Ref.1. Source: RGD protein complex scaffold Inferred from direct assay PubMed 10844024. Source: RGD protein homodimerization activity Inferred from direct assay PubMed 14976213. Source: RGD transferase activity Inferred from electronic annotation. Source: UniProtKB-KW tubulin binding Traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gabra3	P20236	5	EBI-5273276,EBI-5273284

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 6 (identifier: Q03555-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q03555-2)

Also known as: GE124'56;

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MATEGMILTNHDHQIRVGVLTV → MSFPLSPAFTLLHILV

Isoform 2 (identifier: Q03555-3)

Also known as: GE236;

The sequence of this isoform differs from the canonical sequence as follows:
     99-111: Missing.
     256-256: K → KNHPFYTSPAVFMANHGQPIPGLISYSHHATGSADKR
     302-320: Missing.

Isoform 3 (identifier: Q03555-4)

Also known as: GE24'6;

The sequence of this isoform differs from the canonical sequence as follows:
     99-111: Missing.

Isoform 4 (identifier: Q03555-5)

Also known as: GE245;

The sequence of this isoform differs from the canonical sequence as follows:
     302-315: QIRRPDESKGVASR → ARLPSCSSTYSVSE
     316-320: Missing.

Isoform 5 (identifier: Q03555-6)

Also known as: GE26;

The sequence of this isoform differs from the canonical sequence as follows:
     99-111: Missing.
     302-320: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 768

768

Gephyrin

PRO_0000170965

Regions

Region

14 – 166

153

MPT Mo-transferase

Region

153 – 348

196

Interaction with GABARAP

Region

326 – 768

443

MPT adenylyltransferase

Compositional bias

213 – 218

6

Glu-rich (acidic)

Amino acid modifications

Modified residue

201

1

Phosphoserine By similarity

Modified residue

207

1

Phosphoserine By similarity

Modified residue

279

1

Phosphothreonine By similarity

Modified residue

337

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 22

22

MATEG…GVLTV → MSFPLSPAFTLLHILV in isoform 1.

VSP_003238

Alternative sequence

99 – 111

13

Missing in isoform 5, isoform 2 and isoform 3.

VSP_003239

Alternative sequence

256

1

K → KNHPFYTSPAVFMANHGQPI PGLISYSHHATGSADKR in isoform 2.

VSP_003240

Alternative sequence

302 – 320

19

Missing in isoform 5 and isoform 2.

VSP_003243

Alternative sequence

302 – 315

14

QIRRP…GVASR → ARLPSCSSTYSVSE in isoform 4.

VSP_003241

Alternative sequence

316 – 320

5

Missing in isoform 4.

VSP_003242

Experimental info

Mutagenesis

362

1

F → A: Reduced GLRB binding. Ref.9

Mutagenesis

745 – 746

2

PP → AA: Reduced GLRB binding. Ref.9

Mutagenesis

745

1

P → A: Loss of GLRB binding. Ref.9

Sequence conflict

255

1

A → R Ref.3

Secondary structure

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768

Helix Strand Turn

Details...

Beta strand

16 – 22

7

Helix

24 – 27

4

Helix

34 – 44

11

Turn

46 – 49

4

Beta strand

52 – 59

8

Helix

63 – 75

13

Beta strand

80 – 86

7

Beta strand

89 – 91

3

Helix

112 – 116

5

Beta strand

118 – 120

3

Helix

122 – 135

14

Helix

137 – 141

5

Beta strand

146 – 149

4

Beta strand

152 – 157

6

Helix

161 – 171

11

Helix

172 – 174

3

Helix

175 – 182

8

Helix

188 – 193

6

Beta strand

355 – 357

3

Helix

358 – 368

11

Beta strand

374 – 378

5

Helix

379 – 381

3

Beta strand

386 – 389

4

Beta strand

397 – 400

4

Beta strand

402 – 410

9

Helix

412 – 414

3

Beta strand

418 – 424

7

Beta strand

439 – 443

5

Beta strand

455 – 458

4

Helix

459 – 461

3

Beta strand

462 – 468

7

Beta strand

469 – 472

4

Beta strand

474 – 479

6

Turn

485 – 488

4

Beta strand

494 – 496

3

Beta strand

501 – 503

3

Helix

511 – 520

10

Beta strand

524 – 528

5

Beta strand

533 – 538

6

Helix

558 – 567

10

Turn

568 – 570

3

Beta strand

573 – 579

7

Helix

583 – 596

14

Beta strand

598 – 604

7

Beta strand

607 – 609

3

Helix

612 – 619

8

Turn

620 – 622

3

Beta strand

625 – 632

8

Beta strand

640 – 646

7

Beta strand

649 – 656

8

Helix

660 – 678

19

Beta strand

688 – 695

8

Beta strand

704 – 711

8

Beta strand

716 – 718

3

Beta strand

720 – 723

4

Beta strand

728 – 730

3

Beta strand

740 – 744

5

Beta strand

752 – 754

3

Beta strand

759 – 764

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 6 [UniParc]. Last modified May 16, 2006. Version 3. Checksum: FAD1B6DD76ED79EA	FASTA	768	83,266
	10 20 30 40 50 60 MATEGMILTN HDHQIRVGVL TVSDSCFRNL AEDRSGINLK DLVQDPSLLG GTISAYKIVP 70 80 90 100 110 120 DEIEEIKETL IDWCDEKELN LILTTGGTGF APRDVTPEKF PTFPFCGLQK GATKEVIERE 130 140 150 160 170 180 APGMALAMLM GSLNVTPLGM LSRPVCGIRG KTLIINLPGS KKGSQECFQF ILPALPHAID 190 200 210 220 230 240 LLRDAIVKVK EVHDELEDLP SPPPPLSPPP TTSPHKQTED KGVQCEEEEE EKKDSGVAST 250 260 270 280 290 300 EDSSSSHITA AALAAKIPDS IISRGVQVLP RDTASLSTTP SESPRAQATS RLSTASCPTP 310 320 330 340 350 360 KQIRRPDESK GVASRVGSLK VQSRCSSKEN ILRASHSAVD ITKVARRHRM SPFPLTSMDK 370 380 390 400 410 420 AFITVLEMTP VLGTEIINYR DGMGRVLAQD VYAKDNLPPF PASVKDGYAV RAADGPGDRF 430 440 450 460 470 480 IIGESQAGEQ PTQTVMPGQV MRVTTGAPIP CGADAVVQVE DTELIRESDD GTEELEVRIL 490 500 510 520 530 540 VQARPGQDIR PIGHDIKRGE CVLAKGTHMG PSEIGLLATV GVTEVEVNKF PVVAVMSTGN 550 560 570 580 590 600 ELLNPEDDLL PGKIRDSNRS TLLATIQEHG YPTINLGIVG DNPDDLLNAL NEGISRADVI 610 620 630 640 650 660 ITSGGVSMGE KDYLKQVLDI DLHAQIHFGR VFMKPGLPTT FATLDIDGVR KIIFALPGNP 670 680 690 700 710 720 VSAVVTCNLF VVPALRKMQG ILDPRPTIIK ARLSCDVKLD PRPEYHRCIL TWHHQEPLPW 730 740 750 760 AQSTGNQMSS RLMSMRSANG LLMLPPKTEQ YVELHKGEVV DVMVIGRL
	Isoform 1 (GE124'56) [UniParc]. Checksum: CF467AB5D3BE99BE	FASTA	762	82,616
	10 20 30 40 50 60 MSFPLSPAFT LLHILVSDSC FRNLAEDRSG INLKDLVQDP SLLGGTISAY KIVPDEIEEI 70 80 90 100 110 120 KETLIDWCDE KELNLILTTG GTGFAPRDVT PEKFPTFPFC GLQKGATKEV IEREAPGMAL 130 140 150 160 170 180 AMLMGSLNVT PLGMLSRPVC GIRGKTLIIN LPGSKKGSQE CFQFILPALP HAIDLLRDAI 190 200 210 220 230 240 VKVKEVHDEL EDLPSPPPPL SPPPTTSPHK QTEDKGVQCE EEEEEKKDSG VASTEDSSSS 250 260 270 280 290 300 HITAAALAAK IPDSIISRGV QVLPRDTASL STTPSESPRA QATSRLSTAS CPTPKQIRRP 310 320 330 340 350 360 DESKGVASRV GSLKVQSRCS SKENILRASH SAVDITKVAR RHRMSPFPLT SMDKAFITVL 370 380 390 400 410 420 EMTPVLGTEI INYRDGMGRV LAQDVYAKDN LPPFPASVKD GYAVRAADGP GDRFIIGESQ 430 440 450 460 470 480 AGEQPTQTVM PGQVMRVTTG APIPCGADAV VQVEDTELIR ESDDGTEELE VRILVQARPG 490 500 510 520 530 540 QDIRPIGHDI KRGECVLAKG THMGPSEIGL LATVGVTEVE VNKFPVVAVM STGNELLNPE 550 560 570 580 590 600 DDLLPGKIRD SNRSTLLATI QEHGYPTINL GIVGDNPDDL LNALNEGISR ADVIITSGGV 610 620 630 640 650 660 SMGEKDYLKQ VLDIDLHAQI HFGRVFMKPG LPTTFATLDI DGVRKIIFAL PGNPVSAVVT 670 680 690 700 710 720 CNLFVVPALR KMQGILDPRP TIIKARLSCD VKLDPRPEYH RCILTWHHQE PLPWAQSTGN 730 740 750 760 QMSSRLMSMR SANGLLMLPP KTEQYVELHK GEVVDVMVIG RL
	Isoform 2 (GE236) [UniParc]. Checksum: D25D2294184BE3D4	FASTA	772	83,638
	10 20 30 40 50 60 MATEGMILTN HDHQIRVGVL TVSDSCFRNL AEDRSGINLK DLVQDPSLLG GTISAYKIVP 70 80 90 100 110 120 DEIEEIKETL IDWCDEKELN LILTTGGTGF APRDVTPEAT KEVIEREAPG MALAMLMGSL 130 140 150 160 170 180 NVTPLGMLSR PVCGIRGKTL IINLPGSKKG SQECFQFILP ALPHAIDLLR DAIVKVKEVH 190 200 210 220 230 240 DELEDLPSPP PPLSPPPTTS PHKQTEDKGV QCEEEEEEKK DSGVASTEDS SSSHITAAAL 250 260 270 280 290 300 AAKNHPFYTS PAVFMANHGQ PIPGLISYSH HATGSADKRI PDSIISRGVQ VLPRDTASLS 310 320 330 340 350 360 TTPSESPRAQ ATSRLSTASC PTPKVQSRCS SKENILRASH SAVDITKVAR RHRMSPFPLT 370 380 390 400 410 420 SMDKAFITVL EMTPVLGTEI INYRDGMGRV LAQDVYAKDN LPPFPASVKD GYAVRAADGP 430 440 450 460 470 480 GDRFIIGESQ AGEQPTQTVM PGQVMRVTTG APIPCGADAV VQVEDTELIR ESDDGTEELE 490 500 510 520 530 540 VRILVQARPG QDIRPIGHDI KRGECVLAKG THMGPSEIGL LATVGVTEVE VNKFPVVAVM 550 560 570 580 590 600 STGNELLNPE DDLLPGKIRD SNRSTLLATI QEHGYPTINL GIVGDNPDDL LNALNEGISR 610 620 630 640 650 660 ADVIITSGGV SMGEKDYLKQ VLDIDLHAQI HFGRVFMKPG LPTTFATLDI DGVRKIIFAL 670 680 690 700 710 720 PGNPVSAVVT CNLFVVPALR KMQGILDPRP TIIKARLSCD VKLDPRPEYH RCILTWHHQE 730 740 750 760 770 PLPWAQSTGN QMSSRLMSMR SANGLLMLPP KTEQYVELHK GEVVDVMVIG RL
	Isoform 3 (GE24'6) [UniParc]. Checksum: 219EC28CC2910AC1	FASTA	755	81,814
	10 20 30 40 50 60 MATEGMILTN HDHQIRVGVL TVSDSCFRNL AEDRSGINLK DLVQDPSLLG GTISAYKIVP 70 80 90 100 110 120 DEIEEIKETL IDWCDEKELN LILTTGGTGF APRDVTPEAT KEVIEREAPG MALAMLMGSL 130 140 150 160 170 180 NVTPLGMLSR PVCGIRGKTL IINLPGSKKG SQECFQFILP ALPHAIDLLR DAIVKVKEVH 190 200 210 220 230 240 DELEDLPSPP PPLSPPPTTS PHKQTEDKGV QCEEEEEEKK DSGVASTEDS SSSHITAAAL 250 260 270 280 290 300 AAKIPDSIIS RGVQVLPRDT ASLSTTPSES PRAQATSRLS TASCPTPKQI RRPDESKGVA 310 320 330 340 350 360 SRVGSLKVQS RCSSKENILR ASHSAVDITK VARRHRMSPF PLTSMDKAFI TVLEMTPVLG 370 380 390 400 410 420 TEIINYRDGM GRVLAQDVYA KDNLPPFPAS VKDGYAVRAA DGPGDRFIIG ESQAGEQPTQ 430 440 450 460 470 480 TVMPGQVMRV TTGAPIPCGA DAVVQVEDTE LIRESDDGTE ELEVRILVQA RPGQDIRPIG 490 500 510 520 530 540 HDIKRGECVL AKGTHMGPSE IGLLATVGVT EVEVNKFPVV AVMSTGNELL NPEDDLLPGK 550 560 570 580 590 600 IRDSNRSTLL ATIQEHGYPT INLGIVGDNP DDLLNALNEG ISRADVIITS GGVSMGEKDY 610 620 630 640 650 660 LKQVLDIDLH AQIHFGRVFM KPGLPTTFAT LDIDGVRKII FALPGNPVSA VVTCNLFVVP 670 680 690 700 710 720 ALRKMQGILD PRPTIIKARL SCDVKLDPRP EYHRCILTWH HQEPLPWAQS TGNQMSSRLM 730 740 750 SMRSANGLLM LPPKTEQYVE LHKGEVVDVM VIGRL
	Isoform 4 (GE245) [UniParc]. Checksum: 5E6039E40BDA68ED	FASTA	763	82,669
	10 20 30 40 50 60 MATEGMILTN HDHQIRVGVL TVSDSCFRNL AEDRSGINLK DLVQDPSLLG GTISAYKIVP 70 80 90 100 110 120 DEIEEIKETL IDWCDEKELN LILTTGGTGF APRDVTPEKF PTFPFCGLQK GATKEVIERE 130 140 150 160 170 180 APGMALAMLM GSLNVTPLGM LSRPVCGIRG KTLIINLPGS KKGSQECFQF ILPALPHAID 190 200 210 220 230 240 LLRDAIVKVK EVHDELEDLP SPPPPLSPPP TTSPHKQTED KGVQCEEEEE EKKDSGVAST 250 260 270 280 290 300 EDSSSSHITA AALAAKIPDS IISRGVQVLP RDTASLSTTP SESPRAQATS RLSTASCPTP 310 320 330 340 350 360 KARLPSCSST YSVSEVQSRC SSKENILRAS HSAVDITKVA RRHRMSPFPL TSMDKAFITV 370 380 390 400 410 420 LEMTPVLGTE IINYRDGMGR VLAQDVYAKD NLPPFPASVK DGYAVRAADG PGDRFIIGES 430 440 450 460 470 480 QAGEQPTQTV MPGQVMRVTT GAPIPCGADA VVQVEDTELI RESDDGTEEL EVRILVQARP 490 500 510 520 530 540 GQDIRPIGHD IKRGECVLAK GTHMGPSEIG LLATVGVTEV EVNKFPVVAV MSTGNELLNP 550 560 570 580 590 600 EDDLLPGKIR DSNRSTLLAT IQEHGYPTIN LGIVGDNPDD LLNALNEGIS RADVIITSGG 610 620 630 640 650 660 VSMGEKDYLK QVLDIDLHAQ IHFGRVFMKP GLPTTFATLD IDGVRKIIFA LPGNPVSAVV 670 680 690 700 710 720 TCNLFVVPAL RKMQGILDPR PTIIKARLSC DVKLDPRPEY HRCILTWHHQ EPLPWAQSTG 730 740 750 760 NQMSSRLMSM RSANGLLMLP PKTEQYVELH KGEVVDVMVI GRL
	Isoform 5 (GE26) [UniParc]. Checksum: B3817E1BE1D4D41B	FASTA	736	79,748
	10 20 30 40 50 60 MATEGMILTN HDHQIRVGVL TVSDSCFRNL AEDRSGINLK DLVQDPSLLG GTISAYKIVP 70 80 90 100 110 120 DEIEEIKETL IDWCDEKELN LILTTGGTGF APRDVTPEAT KEVIEREAPG MALAMLMGSL 130 140 150 160 170 180 NVTPLGMLSR PVCGIRGKTL IINLPGSKKG SQECFQFILP ALPHAIDLLR DAIVKVKEVH 190 200 210 220 230 240 DELEDLPSPP PPLSPPPTTS PHKQTEDKGV QCEEEEEEKK DSGVASTEDS SSSHITAAAL 250 260 270 280 290 300 AAKIPDSIIS RGVQVLPRDT ASLSTTPSES PRAQATSRLS TASCPTPKVQ SRCSSKENIL 310 320 330 340 350 360 RASHSAVDIT KVARRHRMSP FPLTSMDKAF ITVLEMTPVL GTEIINYRDG MGRVLAQDVY 370 380 390 400 410 420 AKDNLPPFPA SVKDGYAVRA ADGPGDRFII GESQAGEQPT QTVMPGQVMR VTTGAPIPCG 430 440 450 460 470 480 ADAVVQVEDT ELIRESDDGT EELEVRILVQ ARPGQDIRPI GHDIKRGECV LAKGTHMGPS 490 500 510 520 530 540 EIGLLATVGV TEVEVNKFPV VAVMSTGNEL LNPEDDLLPG KIRDSNRSTL LATIQEHGYP 550 560 570 580 590 600 TINLGIVGDN PDDLLNALNE GISRADVIIT SGGVSMGEKD YLKQVLDIDL HAQIHFGRVF 610 620 630 640 650 660 MKPGLPTTFA TLDIDGVRKI IFALPGNPVS AVVTCNLFVV PALRKMQGIL DPRPTIIKAR 670 680 690 700 710 720 LSCDVKLDPR PEYHRCILTW HHQEPLPWAQ STGNQMSSRL MSMRSANGLL MLPPKTEQYV 730 ELHKGEVVDV MVIGRL

References

[1]	"Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein." Prior P., Schmitt B., Grenningloh G., Pribilla I., Multhaup G., Beyreuther K., Maulet Y., Werner P., Langosch D., Kirsch J., Betz H. Neuron 8:1161-1170(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), PARTIAL PROTEIN SEQUENCE. Strain: Wistar. Tissue: Brain.
[2]	Schmitt B. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 255.
[3]	"Functional heterogeneity of gephyrins." Meier J., De Chaldee M., Triller A., Vannier C. Mol. Cell. Neurosci. 16:566-577(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 5).
[4]	"Structural basis of dynamic glycine receptor clustering by gephyrin." Sola M., Bavro V.N., Timmins J., Franz T., Ricard-Blum S., Schoehn G., Ruigrok R.W.H., Paarmann I., Saiyed T., O'Sullivan G.A., Schmitt B., Betz H., Weissenhorn W. EMBO J. 23:2510-2519(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY, X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 348-768 IN COMPLEX WITH GLRB, SUBUNIT.
[5]	"Gephyrin antisense oligonucleotides prevent glycine receptor clustering in spinal neurons." Kirsch J., Wolters I., Triller A., Betz H. Nature 366:745-748(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H., Olsen R.W., Betz H. Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GABARAP.
[7]	"The neurotransmitter receptor-anchoring protein gephyrin reconstitutes molybdenum cofactor biosynthesis in bacteria, plants, and mammalian cells." Stallmeyer B., Schwarz G., Schulze J., Nerlich A., Reiss J., Kirsch J., Mendel R.R. Proc. Natl. Acad. Sci. U.S.A. 96:1333-1338(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"X-ray crystal structure of the trimeric N-terminal domain of gephyrin." Sola M., Kneussel M., Heck I.S., Betz H., Weissenhorn W. J. Biol. Chem. 276:25294-25301(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-201, SUBUNIT.
[9]	"Deciphering the structural framework of glycine receptor anchoring by gephyrin." Kim E.Y., Schrader N., Smolinsky B., Bedet C., Vannier C., Schwarz G., Schindelin H. EMBO J. 25:1385-1395(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 350-768 IN COMPLEX WITH GLRB, SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-362; PRO-745 AND 745-PRO-PRO-746.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X66366 mRNA. Translation: CAA47009.2.

IPI

IPI00211800.
IPI00230795.
IPI00230796.
IPI00230797.
IPI00230798.
IPI00323990.

PIR

JH0681.

RefSeq

NP_074056.2. NM_022865.3.

UniGene

Rn.11032.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IHC	X-ray	1.90	A	1-201	[»]
1T3E	X-ray	3.25	A/B	348-768	[»]
2FTS	X-ray	2.41	A	350-768	[»]
2FU3	X-ray	2.70	A/B	350-768	[»]

ProteinModelPortal

Q03555.

SMR

Q03555. Positions 13-194, 350-768.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-33263N.

IntAct

Q03555. 3 interactions.

PTM databases

PhosphoSite

Q03555.

Proteomic databases

PaxDb

Q03555.

PRIDE

Q03555.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

64845.

KEGG

rno:64845.

UCSC

RGD:69194. rat.

Organism-specific databases

CTD

10243.

RGD

69194. Gphn.

Phylogenomic databases

eggNOG

COG0303.

HOGENOM

HOG000280651.

HOVERGEN

HBG005828.

InParanoid

Q03555.

KO

K15376.

Enzyme and pathway databases

UniPathway

UPA00344.

Gene expression databases

ArrayExpress

Q03555.

Genevestigator

Q03555.

GermOnline

ENSRNOG00000028366. Rattus norvegicus.

Family and domain databases

Gene3D

2.40.340.10. 1 hit.
3.40.980.10. 2 hits.

InterPro

IPR020817. Mo_cofactor_synthesis.
IPR008284. MoCF_biosynth_CS.
IPR005111. MoeA_C_domain_IV.
IPR005110. MoeA_linker/N.
IPR001453. Mopterin-bd_dom.
[Graphical view]

Pfam

PF00994. MoCF_biosynth. 2 hits.
PF03454. MoeA_C. 1 hit.
PF03453. MoeA_N. 1 hit.
[Graphical view]

SMART

SM00852. MoCF_biosynth. 2 hits.
[Graphical view]

SUPFAM

SSF53218. MoCF_biosynth. 2 hits.
SSF63867. MoeA_C. 1 hit.
SSF63882. MoeA_N. 1 hit.

TIGRFAMs

TIGR00177. molyb_syn. 2 hits.

PROSITE

PS01078. MOCF_BIOSYNTHESIS_1. 1 hit.
PS01079. MOCF_BIOSYNTHESIS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q03555.

NextBio

613764.

Entry information

Entry name

GEPH_RAT

Accession

Primary (citable) accession number: Q03555

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 135 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program