ID   GOT1_YEAST              Reviewed;         138 AA.
AC   Q03554; D6W0B9;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 149.
DE   RecName: Full=Protein transport protein GOT1;
DE   AltName: Full=Golgi transport protein 1;
GN   Name=GOT1; OrderedLocusNames=YMR292W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF GLY-73, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=10406798; DOI=10.1093/emboj/18.14.3934;
RA   Conchon S., Cao X., Barlowe C., Pelham H.R.;
RT   "Got1p and Sft2p: membrane proteins involved in traffic to the Golgi
RT   complex.";
RL   EMBO J. 18:3934-3946(1999).
CC   -!- FUNCTION: Nonessential protein required for the fusion of ER-derived
CC       transport vesicles with the Golgi complex. Can be replaced by SFT2.
CC       {ECO:0000269|PubMed:10406798}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:10406798}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:10406798}.
CC   -!- DISRUPTION PHENOTYPE: Lack of GOT1 is lethal in a strain lacking SFT2.
CC       {ECO:0000269|PubMed:10406798}.
CC   -!- SIMILARITY: Belongs to the GOT1 family. {ECO:0000305}.
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DR   EMBL; X80836; CAA56801.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA10193.1; -; Genomic_DNA.
DR   PIR; S47453; S47453.
DR   RefSeq; NP_014020.1; NM_001182800.1.
DR   AlphaFoldDB; Q03554; -.
DR   BioGRID; 35472; 127.
DR   DIP; DIP-8271N; -.
DR   IntAct; Q03554; 15.
DR   MINT; Q03554; -.
DR   STRING; 4932.YMR292W; -.
DR   MaxQB; Q03554; -.
DR   PaxDb; 4932-YMR292W; -.
DR   PeptideAtlas; Q03554; -.
DR   EnsemblFungi; YMR292W_mRNA; YMR292W; YMR292W.
DR   GeneID; 855337; -.
DR   KEGG; sce:YMR292W; -.
DR   AGR; SGD:S000004906; -.
DR   SGD; S000004906; GOT1.
DR   VEuPathDB; FungiDB:YMR292W; -.
DR   eggNOG; KOG1743; Eukaryota.
DR   HOGENOM; CLU_124519_1_0_1; -.
DR   InParanoid; Q03554; -.
DR   OMA; VAMVMGP; -.
DR   OrthoDB; 75938at2759; -.
DR   BioCyc; YEAST:G3O-32962-MONOMER; -.
DR   BioGRID-ORCS; 855337; 0 hits in 10 CRISPR screens.
DR   PRO; PR:Q03554; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; Q03554; Protein.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IDA:SGD.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:SGD.
DR   GO; GO:0000137; C:Golgi cis cisterna; IDA:SGD.
DR   GO; GO:0000139; C:Golgi membrane; IDA:SGD.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IMP:SGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0042147; P:retrograde transport, endosome to Golgi; IGI:SGD.
DR   InterPro; IPR045176; Got1.
DR   InterPro; IPR007305; Vesicle_transpt_Got1/SFT2.
DR   PANTHER; PTHR21493; CGI-141-RELATED/LIPASE CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR21493:SF9; GOLGI TRANSPORT PROTEIN 1-RELATED; 1.
DR   Pfam; PF04178; Got1; 1.
PE   1: Evidence at protein level;
KW   Golgi apparatus; Membrane; Protein transport; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..138
FT                   /note="Protein transport protein GOT1"
FT                   /id="PRO_0000218586"
FT   TOPO_DOM        1..8
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        9..29
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..31
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        53..67
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        89..112
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..133
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        134..138
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         73
FT                   /note="G->S: In got1-1; loss of function. ER-Golgi
FT                   transport defect when associated with lack of SFT2."
FT                   /evidence="ECO:0000269|PubMed:10406798"
SQ   SEQUENCE   138 AA;  15431 MW;  F078F3725D504B7D CRC64;
     MWLTEAQKFG VAFTFGGFLF FLFGIFTFFD RALLALGNIL FLIGVFLIIG SQKTYIFFTR
     PNKRRGSLFF LVGAFLILLK WTFLGFIIES LGIIGLFGDF FGVIVQFLRS MPIIGPILSH
     PAIAPIVDKL AGVRVLPV
//