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Protein

CAAX prenyl protease 2

Gene

RCE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Proteolytically removes the C-terminal three residues of farnesylated proteins, including the a-factor mating pheromone and RAS.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei156Proton donor/acceptorBy similarity1
Active sitei194Proton donor/acceptorBy similarity1
Sitei248Transition state stabilizerBy similarity1
Sitei252Transition state stabilizerBy similarity1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: GO_Central
  • metalloendopeptidase activity Source: SGD

GO - Biological processi

  • CAAX-box protein processing Source: SGD
  • peptide mating pheromone maturation involved in conjugation with cellular fusion Source: SGD
  • response to pheromone Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Pheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-32945-MONOMER.
BRENDAi3.4.99.B1. 984.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiM79.001.
TCDBi9.B.1.2.1. the integral membrane caax protease (caax protease) family.

Names & Taxonomyi

Protein namesi
Recommended name:
CAAX prenyl protease 2 (EC:3.4.22.-)
Alternative name(s):
Prenyl protein-specific endoprotease 2
Short name:
PPSEP 2
Ras and A-factor-converting enzyme
Short name:
RACE
Gene namesi
Name:RCE1
Ordered Locus Names:YMR274C
ORF Names:YM8156.16C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR274C.
SGDiS000004887. RCE1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3LumenalSequence analysis3
Transmembranei4 – 23HelicalSequence analysisAdd BLAST20
Topological domaini24 – 44CytoplasmicSequence analysisAdd BLAST21
Transmembranei45 – 65HelicalSequence analysisAdd BLAST21
Topological domaini66 – 74LumenalSequence analysis9
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 105CytoplasmicSequence analysis10
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Topological domaini127 – 148LumenalSequence analysisAdd BLAST22
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 208CytoplasmicSequence analysisAdd BLAST39
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Topological domaini230 – 237LumenalSequence analysis8
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 275CytoplasmicSequence analysisAdd BLAST17
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 315LumenalSequence analysisAdd BLAST19

GO - Cellular componenti

  • integral component of endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1250413.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948341 – 315CAAX prenyl protease 2Add BLAST315

Proteomic databases

PRIDEiQ03530.

Interactioni

Protein-protein interaction databases

BioGridi35453. 57 interactors.
DIPiDIP-7636N.

Structurei

3D structure databases

ProteinModelPortaliQ03530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase U48 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004124.
HOGENOMiHOG000066056.
InParanoidiQ03530.
KOiK08658.
OMAiGNLWACI.
OrthoDBiEOG092C3L5K.

Family and domain databases

InterProiIPR003675. CAAX_protease.
[Graphical view]
PfamiPF02517. Abi. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQFSTFLVL LYISISYVLP LYATSQPEGS KRDNPRTIKS RMQKLTIMLI
60 70 80 90 100
SNLFLVPFLQ SQLSSTTSHI SFKDAFLGLG IIPGYYAALP NPWQFSQFVK
110 120 130 140 150
DLTKCVAMLL TLYCGPVLDF VLYHLLNPKS SILEDFYHEF LNIWSFRNFI
160 170 180 190 200
FAPITEEIFY TSMLLTTYLN LIPHSQLSYQ QLFWQPSLFF GLAHAHHAYE
210 220 230 240 250
QLQEGSMTTV SILLTTCFQI LYTTLFGGLT KFVFVRTGGN LWCCIILHAL
260 270 280 290 300
CNIMGFPGPS RLNLHFTVVD KKAGRISKLV SIWNKCYFAL LVLGLISLKD
310
TLQTLVGTPG YRITL
Length:315
Mass (Da):35,911
Last modified:November 1, 1997 - v1
Checksum:iD151F4790F066081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49260 Genomic DNA. Translation: CAA89257.1.
BK006946 Genomic DNA. Translation: DAA10175.1.
PIRiS54486.
RefSeqiNP_014001.1. NM_001182781.1.

Genome annotation databases

EnsemblFungiiYMR274C; YMR274C; YMR274C.
GeneIDi855317.
KEGGisce:YMR274C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49260 Genomic DNA. Translation: CAA89257.1.
BK006946 Genomic DNA. Translation: DAA10175.1.
PIRiS54486.
RefSeqiNP_014001.1. NM_001182781.1.

3D structure databases

ProteinModelPortaliQ03530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35453. 57 interactors.
DIPiDIP-7636N.

Chemistry databases

ChEMBLiCHEMBL1250413.

Protein family/group databases

MEROPSiM79.001.
TCDBi9.B.1.2.1. the integral membrane caax protease (caax protease) family.

Proteomic databases

PRIDEiQ03530.

Protocols and materials databases

DNASUi855317.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR274C; YMR274C; YMR274C.
GeneIDi855317.
KEGGisce:YMR274C.

Organism-specific databases

EuPathDBiFungiDB:YMR274C.
SGDiS000004887. RCE1.

Phylogenomic databases

GeneTreeiENSGT00390000004124.
HOGENOMiHOG000066056.
InParanoidiQ03530.
KOiK08658.
OMAiGNLWACI.
OrthoDBiEOG092C3L5K.

Enzyme and pathway databases

BioCyciYEAST:G3O-32945-MONOMER.
BRENDAi3.4.99.B1. 984.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.

Miscellaneous databases

PROiQ03530.

Family and domain databases

InterProiIPR003675. CAAX_protease.
[Graphical view]
PfamiPF02517. Abi. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCE1_YEAST
AccessioniPrimary (citable) accession number: Q03530
Secondary accession number(s): D6W0A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.