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Q03530 (RCE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CAAX prenyl protease 2
EC=3.4.22.-
Alternative name(s):
Prenyl protein-specific endoprotease 2
Short name=PPSEP 2
Ras and A-factor-converting enzyme
Short name=RACE
Gene names
Name:RCE1
Ordered Locus Names:YMR274C
ORF Names:YM8156.16C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proteolytically removes the C-terminal three residues of farnesylated a-factor mating pheromone and of RAS. Ref.3

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.4.

Sequence similarities

Belongs to the peptidase U48 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315CAAX prenyl protease 2
PRO_0000194834

Regions

Topological domain1 – 33Lumenal Potential
Transmembrane4 – 2320Helical; Potential
Topological domain24 – 4421Cytoplasmic Potential
Transmembrane45 – 6521Helical; Potential
Topological domain66 – 749Lumenal Potential
Transmembrane75 – 9521Helical; Potential
Topological domain96 – 10510Cytoplasmic Potential
Transmembrane106 – 12621Helical; Potential
Topological domain127 – 14822Lumenal Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 20839Cytoplasmic Potential
Transmembrane209 – 22921Helical; Potential
Topological domain230 – 2378Lumenal Potential
Transmembrane238 – 25821Helical; Potential
Topological domain259 – 27517Cytoplasmic Potential
Transmembrane276 – 29621Helical; Potential
Topological domain297 – 31519Lumenal Potential

Sequences

Sequence LengthMass (Da)Tools
Q03530 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: D151F4790F066081

FASTA31535,911
        10         20         30         40         50         60 
MLQFSTFLVL LYISISYVLP LYATSQPEGS KRDNPRTIKS RMQKLTIMLI SNLFLVPFLQ 

        70         80         90        100        110        120 
SQLSSTTSHI SFKDAFLGLG IIPGYYAALP NPWQFSQFVK DLTKCVAMLL TLYCGPVLDF 

       130        140        150        160        170        180 
VLYHLLNPKS SILEDFYHEF LNIWSFRNFI FAPITEEIFY TSMLLTTYLN LIPHSQLSYQ 

       190        200        210        220        230        240 
QLFWQPSLFF GLAHAHHAYE QLQEGSMTTV SILLTTCFQI LYTTLFGGLT KFVFVRTGGN 

       250        260        270        280        290        300 
LWCCIILHAL CNIMGFPGPS RLNLHFTVVD KKAGRISKLV SIWNKCYFAL LVLGLISLKD 

       310 
TLQTLVGTPG YRITL

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Modulation of Ras and a-factor function by carboxyl-terminal proteolysis."
Boyartchuk V.L., Ashby M.N., Rine J.
Science 275:1796-1800(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Endoplasmic reticulum membrane localization of Rce1p and Ste24p, yeast proteases involved in carboxyl-terminal CAAX protein processing and amino-terminal a-factor cleavage."
Schmidt W.K., Tam A., Fujimura-Kamada K., Michaelis S.
Proc. Natl. Acad. Sci. U.S.A. 95:11175-11180(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"The CaaX proteases, Afc1p and Rce1p, have overlapping but distinct substrate specificities."
Trueblood C.E., Boyartchuk V.L., Picologlou E.A., Rozema D., Poulter C.D., Rine J.
Mol. Cell. Biol. 20:4381-4392(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z49260 Genomic DNA. Translation: CAA89257.1.
BK006946 Genomic DNA. Translation: DAA10175.1.
PIRS54486.
RefSeqNP_014001.1. NM_001182781.1.

3D structure databases

ProteinModelPortalQ03530.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-7636N.
STRING4932.YMR274C.

Protein family/group databases

MEROPSM79.001.

Proteomic databases

PaxDbQ03530.

Protocols and materials databases

DNASU855317.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR274C; YMR274C; YMR274C.
GeneID855317.
KEGGsce:YMR274C.

Organism-specific databases

CYGDYMR274c.
SGDS000004887. RCE1.

Phylogenomic databases

eggNOGNOG264307.
GeneTreeENSGT00390000004124.
HOGENOMHOG000066056.
KOK08658.
OMALFGIAHV.
OrthoDBEOG4ZCXF1.

Gene expression databases

GenevestigatorQ03530.
GermOnlineYMR274C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR003675. CAAX_protease.
[Graphical view]
PfamPF02517. Abi. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChEMBLCHEMBL1250413.
NextBio979014.

Entry information

Entry nameRCE1_YEAST
AccessionPrimary (citable) accession number: Q03530
Secondary accession number(s): D6W0A1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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