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Protein

CAAX prenyl protease 2

Gene

RCE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proteolytically removes the C-terminal three residues of farnesylated proteins, including the a-factor mating pheromone and RAS.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei156Proton donor/acceptorBy similarity1
Active sitei194Proton donor/acceptorBy similarity1
Sitei248Transition state stabilizerBy similarity1
Sitei252Transition state stabilizerBy similarity1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: GO_Central
  • metalloendopeptidase activity Source: SGD

GO - Biological processi

  • CAAX-box protein processing Source: SGD
  • peptide mating pheromone maturation involved in posititve regulation of conjugation with cellular fusion Source: SGD
  • response to pheromone Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Protease
Biological processPheromone response

Enzyme and pathway databases

BioCyciYEAST:G3O-32945-MONOMER
BRENDAi3.4.99.B1 984
ReactomeiR-SCE-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiM79.001
TCDBi9.B.1.2.1 the integral membrane caax protease (caax protease) family

Names & Taxonomyi

Protein namesi
Recommended name:
CAAX prenyl protease 2 (EC:3.4.22.-)
Alternative name(s):
Prenyl protein-specific endoprotease 2
Short name:
PPSEP 2
Ras and A-factor-converting enzyme
Short name:
RACE
Gene namesi
Name:RCE1
Ordered Locus Names:YMR274C
ORF Names:YM8156.16C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR274C
SGDiS000004887 RCE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3LumenalSequence analysis3
Transmembranei4 – 23HelicalSequence analysisAdd BLAST20
Topological domaini24 – 44CytoplasmicSequence analysisAdd BLAST21
Transmembranei45 – 65HelicalSequence analysisAdd BLAST21
Topological domaini66 – 74LumenalSequence analysis9
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 105CytoplasmicSequence analysis10
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Topological domaini127 – 148LumenalSequence analysisAdd BLAST22
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 208CytoplasmicSequence analysisAdd BLAST39
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Topological domaini230 – 237LumenalSequence analysis8
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 275CytoplasmicSequence analysisAdd BLAST17
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 315LumenalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1250413

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948341 – 315CAAX prenyl protease 2Add BLAST315

Proteomic databases

PaxDbiQ03530
PRIDEiQ03530

Interactioni

Protein-protein interaction databases

BioGridi35453, 156 interactors
DIPiDIP-7636N
STRINGi4932.YMR274C

Structurei

3D structure databases

ProteinModelPortaliQ03530
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase U48 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004124
HOGENOMiHOG000066056
InParanoidiQ03530
KOiK08658
OMAiHMICNIF
OrthoDBiEOG092C3L5K

Family and domain databases

InterProiView protein in InterPro
IPR003675 CAAX_protease
PfamiView protein in Pfam
PF02517 Abi, 1 hit

Sequencei

Sequence statusi: Complete.

Q03530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQFSTFLVL LYISISYVLP LYATSQPEGS KRDNPRTIKS RMQKLTIMLI
60 70 80 90 100
SNLFLVPFLQ SQLSSTTSHI SFKDAFLGLG IIPGYYAALP NPWQFSQFVK
110 120 130 140 150
DLTKCVAMLL TLYCGPVLDF VLYHLLNPKS SILEDFYHEF LNIWSFRNFI
160 170 180 190 200
FAPITEEIFY TSMLLTTYLN LIPHSQLSYQ QLFWQPSLFF GLAHAHHAYE
210 220 230 240 250
QLQEGSMTTV SILLTTCFQI LYTTLFGGLT KFVFVRTGGN LWCCIILHAL
260 270 280 290 300
CNIMGFPGPS RLNLHFTVVD KKAGRISKLV SIWNKCYFAL LVLGLISLKD
310
TLQTLVGTPG YRITL
Length:315
Mass (Da):35,911
Last modified:November 1, 1997 - v1
Checksum:iD151F4790F066081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49260 Genomic DNA Translation: CAA89257.1
BK006946 Genomic DNA Translation: DAA10175.1
PIRiS54486
RefSeqiNP_014001.1, NM_001182781.1

Genome annotation databases

EnsemblFungiiYMR274C; YMR274C; YMR274C
GeneIDi855317
KEGGisce:YMR274C

Similar proteinsi

Entry informationi

Entry nameiRCE1_YEAST
AccessioniPrimary (citable) accession number: Q03530
Secondary accession number(s): D6W0A1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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