Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q03529 (SCS7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ceramide very long chain fatty acid hydroxylase SCS7

Short name=Ceramide VLCFA hydroxylase SCS7
EC=1.-.-.-
Alternative name(s):
Suppressor of calcium sensitivity 7
Gene names
Name:SCS7
Synonyms:FAH1
Ordered Locus Names:YMR272C
ORF Names:YM8156.14C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ceramide hydroxylase involved in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids. Hydroxylates the very long chain fatty acid of ceramides at C2 and C3. Ref.4 Ref.5 Ref.6 Ref.7 Ref.11 Ref.12

Cofactor

Iron By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.8.

Domain

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Miscellaneous

Present with 3290 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the sterol desaturase family. SCS7 subfamily.

Contains 1 cytochrome b5 heme-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Ceramide very long chain fatty acid hydroxylase SCS7
PRO_0000185407

Regions

Topological domain1 – 196196Cytoplasmic Potential
Transmembrane197 – 21721Helical; Potential
Topological domain218 – 2225Lumenal Potential
Transmembrane223 – 24321Helical; Potential
Topological domain244 – 29754Cytoplasmic Potential
Transmembrane298 – 31821Helical; Potential
Topological domain319 – 35234Lumenal Potential
Transmembrane353 – 37321Helical; Potential
Topological domain374 – 38411Cytoplasmic Potential
Domain9 – 9082Cytochrome b5 heme-binding

Sites

Metal binding451Iron (heme axial ligand) By similarity
Metal binding721Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03529 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: DF4BA5F2E0EA2218

FASTA38444,881
        10         20         30         40         50         60 
MSTNTSKTLE LFSKKTVQEH NTANDCWVTY QNRKIYDVTR FLSEHPGGDE SILDYAGKDI 

        70         80         90        100        110        120 
TEIMKDSDVH EHSDSAYEIL EDEYLIGYLA TDEEAARLLT NKNHKVEVQL SADGTEFDST 

       130        140        150        160        170        180 
TFVKELPAEE KLSIATDYSN DYKKHKFLDL NRPLLMQILR SDFKKDFYVD QIHRPRHYGK 

       190        200        210        220        230        240 
GSAPLFGNFL EPLTKTAWWV VPVAWLPVVV YHMGVALKNM NQLFACFLFC VGVFVWTLIE 

       250        260        270        280        290        300 
YGLHRFLFHF DDWLPESNIA FATHFLLHGC HHYLPMDKYR LVMPPTLFVI LCAPFYKLVF 

       310        320        330        340        350        360 
ALLPLYWAYA GFAGGLFGYV CYDECHFFLH HSKLPPFMRK LKKYHLEHHY KNYQLGFGVT 

       370        380 
SWFWDEVFGT YLGPDAPLSK MKYE 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Fah1p, a Saccharomyces cerevisiae cytochrome b5 fusion protein, and its Arabidopsis thaliana homolog that lacks the cytochrome b5 domain both function in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids."
Mitchell A.G., Martin C.E.
J. Biol. Chem. 272:28281-28288(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and Scs7p."
Haak D., Gable K., Beeler T., Dunn T.
J. Biol. Chem. 272:29704-29710(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Synthesis of monohydroxylated inositolphosphorylceramide (IPC-C) in Saccharomyces cerevisiae requires Scs7p, a protein with both a cytochrome b5-like domain and a hydroxylase/desaturase domain."
Dunn T.M., Haak D., Monaghan E., Beeler T.J.
Yeast 14:311-321(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Sterol-dependent regulation of sphingolipid metabolism in Saccharomyces cerevisiae."
Swain E., Baudry K., Stukey J., McDonough V., Germann M., Nickels J.T. Jr.
J. Biol. Chem. 277:26177-26184(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[11]"Mass spectrometry-based profiling of phospholipids and sphingolipids in extracts from Saccharomyces cerevisiae."
Guan X.L., Wenk M.R.
Yeast 23:465-477(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro."
Bosson R., Guillas I., Vionnet C., Roubaty C., Conzelmann A.
Eukaryot. Cell 8:306-314(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49260 Genomic DNA. Translation: CAA89255.1.
AY693150 Genomic DNA. Translation: AAT93169.1.
BK006946 Genomic DNA. Translation: DAA10172.1.
PIRS54484.
RefSeqNP_013999.1. NM_001182779.1.

3D structure databases

ProteinModelPortalQ03529.
SMRQ03529. Positions 13-85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35451. 522 interactions.
IntActQ03529. 34 interactions.
MINTMINT-4498866.
STRING4932.YMR272C.

Proteomic databases

MaxQBQ03529.
PaxDbQ03529.
PeptideAtlasQ03529.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR272C; YMR272C; YMR272C.
GeneID855315.
KEGGsce:YMR272C.

Organism-specific databases

CYGDYMR272c.
SGDS000004885. SCS7.

Phylogenomic databases

eggNOGCOG3000.
GeneTreeENSGT00390000002142.
HOGENOMHOG000023981.
OMAMKAHHVK.
OrthoDBEOG7RNK98.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13537.
YEAST:YMR272C-MONOMER.

Gene expression databases

GenevestigatorQ03529.

Family and domain databases

Gene3D3.10.120.10. 1 hit.
InterProIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR006694. Fatty_acid_hydroxylase.
IPR014430. Ino-phos-ceramide-B_Hydrxlase.
[Graphical view]
PfamPF00173. Cyt-b5. 1 hit.
PF04116. FA_hydroxylase. 1 hit.
[Graphical view]
PIRSFPIRSF005149. IPC-B_HD. 1 hit.
PRINTSPR00363. CYTOCHROMEB5.
SUPFAMSSF55856. SSF55856. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979008.
PROQ03529.

Entry information

Entry nameSCS7_YEAST
AccessionPrimary (citable) accession number: Q03529
Secondary accession number(s): D6W098
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families