Reviewed, UniProtKB/Swiss-Prot Q03523 (MURE_BACSU)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	BSU15180

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity HAMAP MF_00208.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_0000101866
Regions
	Nucleotide binding	109 – 115	7	ATP Potential
	Region	151 – 152	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	408 – 411	4	Meso-diaminopimelate binding By similarity
	Motif	408 – 411	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	31	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	150	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	178	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	186	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	384	1	Meso-diaminopimelate By similarity
	Binding site	458	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	462	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	218	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03523-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: AD5458EB6DF1C1B0	FASTA	494	54,326
	10 20 30 40 50 60 MKLTKLLTYL TTEPSVNDSQ DPEITSIEMD SREVKKGSLF VCVKGYTVDG HDFAQKAVEN 70 80 90 100 110 120 GAAAIVAERE VDVNVPVIIV RQSLRALSVL SDAFYGQPTK KLQLIGITGT NGKTSTTHMV 130 140 150 160 170 180 DEILKKAGKR TGLIGTMYMK IGDETLPVKN TTPESVTLQK TFKKMNDKHV DTAIMEVSSH 190 200 210 220 230 240 ALSLGRVHGC DYDIAVFTNL TQDHLDYHKT MDEYRHAKSL LFSQLGGAFN HEHPKRAVLN 250 260 270 280 290 300 ADDEASAYFE KVTAAHISTY GIKNDADVMA KNISITAQGT SFDLVTNKGT KHITMSLVGQ 310 320 330 340 350 360 FNVYNVLAAV ATCIAAGIPF EIITEAVEEL HGVRGRFELV NQQQEFPVIV DYAHTPDSLE 370 380 390 400 410 420 NVLETCRDMT EGKLFVVVGC GGDRDKTKRP KMAKIAVELA DEPIFTSDNP RSEDPRAILR 430 440 450 460 470 480 DMEAGVENAY YHSIANREQA IFFAIANAKK GDVVLIAGKG HETYQQIGNE TFDFDDAEVA 490 ARAIVELNKN KTNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of the murE-murD region of Bacillus subtilis 168." Daniel R.A., Errington J. J. Gen. Microbiol. 139:361-370(1993) [PubMed: 8436954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"The Bacillus subtilis spoVD gene encodes a mother-cell-specific penicillin-binding protein required for spore morphogenesis." Daniel R.A., Drake S., Buchanan C.E., Scholle R., Errington J. J. Mol. Biol. 235:209-220(1994) [PubMed: 8289242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z15056 Genomic DNA. Translation: CAA78767.1. AL009126 Genomic DNA. Translation: CAB13391.1. Z25865 Genomic DNA. Translation: CAA81086.1.
PIR	B47691.
RefSeq	NP_389401.1.
3D structure databases
SMR	Q03523. Positions 13-490.
ModBase	Search...
Genome annotation databases
GeneID	937567.
GenomeReviews	Gene locus BSU15180 in contig AL009126_GR.
KEGG	bsu:BSU15180.
NMPDR	fig|224308.1.peg.1520.
Organism-specific databases
SubtiList	BG10223. murE. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG602753.
OMA	EHLDFHG.
PhylomeDB	Q03523.
Enzyme and pathway databases
BRENDA	6.3.2.13. 150.
Family and domain databases
HAMAP	MF_00208. MurE. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_BACSU

Accession

Primary (citable) accession number: Q03523

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	February 9, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)