Reviewed, UniProtKB/Swiss-Prot Q03522 (MURD_BACSU)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BSU15200

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108966
Regions
	Nucleotide binding	120 – 126	7	ATP Potential
Experimental info
	Sequence conflict	342	1	A → R in CAA45555. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q03522-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 66594D6FF91A3932	FASTA	451	49,651
	10 20 30 40 50 60 MENDQFLQKQ HFLILGLAKS GYAAASILHE KGIYVAVNDQ KPFEENEPAQ KLSEKGIEVV 70 80 90 100 110 120 CGEHPVSLFD QHQITILIKN PGIPYENIMV QEAEKRGIPV WTEIELAYYL TSAKFIGITG 130 140 150 160 170 180 SNGKTTTTTL IYEMLKADSQ KALIAGNIGT VASEVAYHAD GDEWIVTELS SFQLMGTHAF 190 200 210 220 230 240 RPEISLILNV FDAHLDYHHT RENYEKAKQK VYLHQTASDK AIVNQDDETV VRLAEAGKAE 250 260 270 280 290 300 IVPFSVSKTL EQGAYVKDSM IMFNGEAILP LEEVVLPGAH NLENILAAIA VVKTAGASNE 310 320 330 340 350 360 AVKKVLTSFT GVKHRLQYVT TVNGRKFYND SKATNILATS KALSAFDKPV ILLAGGLDRG 370 380 390 400 410 420 NGFDDLKPYM KHVKAVLTFG QTAPKLEKLG NELGIQHVKR VDNVEQAVSA AFALSNEGDV 430 440 450 ILLSPACASW DQFKTFEERG DMFIDAVHML K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of the murE-murD region of Bacillus subtilis 168." Daniel R.A., Errington J. J. Gen. Microbiol. 139:361-370(1993) [PubMed: 8436954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"A Bacillus subtilis morphogene cluster that includes spoVE is homologous to the mra region of Escherichia coli." Henriques A.O., de Lencastre H., Piggot P.J. Biochimie 74:735-748(1992) [PubMed: 1391053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 82-451. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z15056 Genomic DNA. Translation: CAA78769.1. AL009126 Genomic DNA. Translation: CAB13393.1. X64258 Genomic DNA. Translation: CAA45555.1.
PIR	D47691.
RefSeq	NP_389403.1.
3D structure databases
SMR	Q03522. Positions 7-450.
ModBase	Search...
Genome annotation databases
GeneID	935950.
GenomeReviews	Gene locus BSU15200 in contig AL009126_GR.
KEGG	bsu:BSU15200.
NMPDR	fig|224308.1.peg.1522.
Organism-specific databases
SubtiList	BG10225. murD. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	VKYYNDS.
PhylomeDB	Q03522.
Enzyme and pathway databases
BRENDA	6.3.2.9. 150.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BACSU

Accession

Primary (citable) accession number: Q03522
Secondary accession number(s): Q59246

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	February 9, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)