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Q03522 (MURD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene names
Name:murD
Ordered Locus Names:BSU15200
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length451 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity HAMAP MF_00639.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 451451UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_0000108966

Regions

Nucleotide binding120 – 1267ATP Potential

Experimental info

Sequence conflict3421A → R in CAA45555. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q03522 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 66594D6FF91A3932

FASTA45149,651
        10         20         30         40         50         60 
MENDQFLQKQ HFLILGLAKS GYAAASILHE KGIYVAVNDQ KPFEENEPAQ KLSEKGIEVV 

        70         80         90        100        110        120 
CGEHPVSLFD QHQITILIKN PGIPYENIMV QEAEKRGIPV WTEIELAYYL TSAKFIGITG 

       130        140        150        160        170        180 
SNGKTTTTTL IYEMLKADSQ KALIAGNIGT VASEVAYHAD GDEWIVTELS SFQLMGTHAF 

       190        200        210        220        230        240 
RPEISLILNV FDAHLDYHHT RENYEKAKQK VYLHQTASDK AIVNQDDETV VRLAEAGKAE 

       250        260        270        280        290        300 
IVPFSVSKTL EQGAYVKDSM IMFNGEAILP LEEVVLPGAH NLENILAAIA VVKTAGASNE 

       310        320        330        340        350        360 
AVKKVLTSFT GVKHRLQYVT TVNGRKFYND SKATNILATS KALSAFDKPV ILLAGGLDRG 

       370        380        390        400        410        420 
NGFDDLKPYM KHVKAVLTFG QTAPKLEKLG NELGIQHVKR VDNVEQAVSA AFALSNEGDV 

       430        440        450 
ILLSPACASW DQFKTFEERG DMFIDAVHML K

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence of the murE-murD region of Bacillus subtilis 168."
Daniel R.A., Errington J.
J. Gen. Microbiol. 139:361-370(1993) [PubMed: 8436954] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"A Bacillus subtilis morphogene cluster that includes spoVE is homologous to the mra region of Escherichia coli."
Henriques A.O., de Lencastre H., Piggot P.J.
Biochimie 74:735-748(1992) [PubMed: 1391053] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 82-451.
Strain: 168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z15056 Genomic DNA. Translation: CAA78769.1.
AL009126 Genomic DNA. Translation: CAB13393.1.
X64258 Genomic DNA. Translation: CAA45555.1.
PIRD47691.
RefSeqNP_389403.1. NC_000964.3.

3D structure databases

ProteinModelPortalQ03522.
SMRQ03522. Positions 6-451.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000001305; EBBACP00000001305; EBBACG00000001303.
GeneID935950.
GenomeReviewsGene locus BSU15200 in contig AL009126_GR.
KEGGbsu:BSU15200.
NMPDRfig|224308.1.peg.1522.
PATRIC18974845. VBIBacSub10457_1614.

Organism-specific databases

GenoListBSU15200. [Micado]

Phylogenomic databases

GeneTreeEBGT00070000032130.
HOGENOMHBG750024.
OMAACASWDM.
PhylomeDBQ03522.
ProtClustDBPRK02472.

Enzyme and pathway databases

BioCycBSUB:BSU15200-MONOMER.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01925.
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. MurD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_BACSU
AccessionPrimary (citable) accession number: Q03522
Secondary accession number(s): Q59246
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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