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Q03521 (MRAY_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phospho-N-acetylmuramoyl-pentapeptide-transferase
EC=2.7.8.13
Alternative name(s):
UDP-MurNAc-pentapeptide phosphotransferase
Gene names
Name:mraY
Ordered Locus Names:BSU15190
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan. HAMAP MF_00038

Catalytic activity

UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00038.

Sequence similarities

Belongs to the glycosyltransferase 4 family. MraY subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038
PRO_0000108783

Regions

Transmembrane5 – 2521Helical; Potential
Transmembrane50 – 7021Helical; Potential
Transmembrane77 – 9721Helical; Potential
Transmembrane117 – 13721Helical; Potential
Transmembrane147 – 16721Helical; Potential
Transmembrane176 – 19621Helical; Potential
Transmembrane203 – 22321Helical; Potential
Transmembrane227 – 24721Helical; Potential
Transmembrane250 – 27021Helical; Potential
Transmembrane302 – 32221Helical; Potential

Experimental info

Sequence conflict222 – 2232AH → RD in CAA78768. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q03521 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: 91246A8B8CAF05BD

FASTA32435,527
        10         20         30         40         50         60 
MLEQVILFTI LMGFLISVLL SPILIPFLRR LKFGQSIREE GPKSHQKKSG TPTMGGVMII 

        70         80         90        100        110        120 
LSIIVTTIVM TQKFSEISPE MVLLLFVTLG YGLLGFLDDY IKVVMKRNLG LTSKQKLIGQ 

       130        140        150        160        170        180 
IIIAVVFYAV YHYYNFATDI RIPGTDLSFD LGWAYFILVL FMLVGGSNAV NLTDGLDGLL 

       190        200        210        220        230        240 
SGTAAIAFGA FAILAWNQSQ YDVAIFSVAV VGAVLGFLVF NAHPAKVFMG DTGSLALGGA 

       250        260        270        280        290        300 
IVTIAILTKL EILLVIIGGV FVIETLSVIL QVISFKTTGK RIFKMSPLHH HYELVGWSEW 

       310        320 
RVVVTFWAAG LLLAVLGIYI EVWL

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence of the murE-murD region of Bacillus subtilis 168."
Daniel R.A., Errington J.
J. Gen. Microbiol. 139:361-370(1993) [PubMed: 8436954] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 222-223.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z15056 Genomic DNA. Translation: CAA78768.1.
AL009126 Genomic DNA. Translation: CAB13392.2.
PIRC47691.
RefSeqNP_389402.2. NC_000964.3.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000002534; EBBACP00000002534; EBBACG00000002529.
GeneID939856.
GenomeReviewsGene locus BSU15190 in contig AL009126_GR.
KEGGbsu:BSU15190.
NMPDRfig|224308.1.peg.1521.
PATRIC18974843. VBIBacSub10457_1613.

Organism-specific databases

GenoListBSU15190. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000001191.
HOGENOMHBG708263.
PhylomeDBQ03521.
ProtClustDBPRK00108.

Enzyme and pathway databases

BioCycBSUB:BSU15190-MONOMER.

Family and domain databases

HAMAPMF_00038. MraY.
[Tree]
InterProIPR000715. Glycosyl_transferase_4.
IPR003524. PNAcMuramoyl-5peptid_Trfase.
IPR018480. PNAcMuramoyl-5peptid_Trfase_CS.
[Graphical view]
KOK01000.
PANTHERPTHR22926. Glyco_trans_4. 1 hit.
PTHR22926:SF3. PNAcPpept_trans. 1 hit.
PfamPF00953. Glycos_transf_4. 1 hit.
PF10555. MraY_sig1. 1 hit.
[Graphical view]
TIGRFAMsTIGR00445. MraY. 1 hit.
PROSITEPS01347. MRAY_1. 1 hit.
PS01348. MRAY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMRAY_BACSU
AccessionPrimary (citable) accession number: Q03521
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 16, 2009
Last modified: January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents