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Reviewed, UniProtKB/Swiss-Prot Q03520 (PCPC_SPHCR)

Last modified March 2, 2010. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
Tetrachloro-P-hydroquinone reductive dehalogenase
EC=2.5.1.-
Gene names
Name:pcpC
OrganismSphingobium chlorophenolicum
Taxonomic identifier46429 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingobium
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Protein attributesHide

Sequence length248 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Function

Sequential reduction of tetrachloro-p-hydroquinone to monochlorophenol, using glutathione as the reducing agent. Ref.2

Pathway

Xenobiotic degradation; pentachlorophenol degradation.

Subunit structure

Homodimer.

Induction

Constitutively expressed.

Sequence similarities

Belongs to the GST superfamily.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

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OntologiesHide

Keywords
   Biological processAromatic hydrocarbons catabolism
   Molecular functionTransferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functiontransferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...
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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 248247Tetrachloro-P-hydroquinone reductive dehalogenase
PRO_0000185991

Regions

Domain2 – 8483GST N-terminal
Domain133 – 248116GST C-terminal
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SequencesHide

Sequence LengthMass (Da)Tools
Q03520-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A6B4A59A65F8AC77

FASTA24828,246
        10         20         30         40         50         60 
MPEVSLYNYT MSICSMKTRL AMEEFGVDYD DKQVDIGFAL ENFEPDYVRL NEKAVVPTLV 

        70         80         90        100        110        120 
VGDRVVTNSY NIVLEAAKLG KVGIPADPVE NKAALDWFQK GDQVNFQVIT YGHKGVPRGD 

       130        140        150        160        170        180 
ELLIARRERA KEYAEKYPEL RSIYQAAHDR IVEHGNCAYD ADTVAQAEVD LQKRLDELDA 

       190        200        210        220        230        240 
HLADKPFIAG SNYSIADIMW TVLLARIEML NMTAWISERP NLLAYYQRMK ARRSFETARV 


MPNWKGGI

« Hide

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ReferencesHide

[1]"Characterization of a Flavobacterium glutathione S-transferase gene involved reductive dechlorination."
Orser C.S., Dutton J., Lange C.C., Jablonski P.E., Xun L., Hargis M.
J. Bacteriol. 175:2640-2644(1993) [PubMed: 8478329] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 39723 / DSM 6824 / L-1.
[2]"Organization and regulation of pentachlorophenol-degrading genes in Sphingobium chlorophenolicum ATCC 39723."
Cai M., Xun L.
J. Bacteriol. 184:4672-4680(2002) [PubMed: 12169590] [Abstract]
Cited for: SEQUENCE REVISION TO 78-79 AND 180, FUNCTION.
Strain: ATCC 39723 / DSM 6824 / L-1.
[3]"Purification and characterization of a tetrachloro-p-hydroquinone reductive dehalogenase from a Flavobacterium sp."
Xun L., Topp E., Orser C.S.
J. Bacteriol. 174:8003-8007(1992) [PubMed: 1459949] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-19, CHARACTERIZATION.
Strain: ATCC 39723 / DSM 6824 / L-1.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ		AF512952 Genomic DNA. Translation: AAM96673.1.

3D structure databases

ModBaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:PCPCFLAVO-MONOMER.

Family and domain databases

InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR012335. Thioredoxin_fold.
[Graphical view]
Gene3DG3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...
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Entry informationHide

Entry namePCPC_SPHCR
AccessionPrimary (citable) accession number: Q03520
Secondary accession number(s): Q8KN34
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: March 2, 2010
This is version 53 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
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Relevant documentsHide

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents