Reviewed, UniProtKB/Swiss-Prot Q03515 (NAR1_RABIT)

Names and origin

Protein names

Recommended name:
    GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
    EC=2.4.2.31
Alternative name(s):
    Mono(ADP-ribosyl)transferase
    CD_antigen=CD296

Gene names

Name:

ART1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Sarcoplasmic reticulum membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Primarily in skeletal and cardiac muscle.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Membrane Sarcoplasmic reticulum
Domain	Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell sarcoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD(P)+-protein-arginine ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 295	273	GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1		PRO_0000019315
	Propeptide	296 – 327	32	Removed in mature form Potential		PRO_0000019316
Sites
	Active site	247	1	By similarity
	Binding site	121	1	NAD By similarity
	Binding site	179	1	NAD By similarity
	Binding site	233	1	NAD By similarity
Amino acid modifications
	Lipidation	295	1	GPI-anchor amidated serine Potential
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Disulfide bond	53 ↔ 277		By similarity
	Disulfide bond	174 ↔ 224		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03515-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 3D2E4E005264A187	FASTA	327	36,134
	10 20 30 40 50 60 MWVPAVANLL LLSLGLLEAI QAQSHLVTRR DLFSQETPLD MAPASFDDQY VGCAAAMTAA 70 80 90 100 110 120 LPHLNLTEFQ VNKVYADGWA LASSQWRERS AWGPEWGLST TRLPPPPAGF RDEHGVALLA 130 140 150 160 170 180 YTANSPLHKE FNAAVRQAGR SRAHYLQHFS FKTLHFLLTE ALQLLGRDQR MPRCRQVFRG 190 200 210 220 230 240 VHGLRFRPAG PGTTVRLGGF ASASLKNVAA QQFGEDTFFG IWTCLGVPIQ GYSFFPGEEE 250 260 270 280 290 300 VLIPPFETFQ VINASRPAQG PARIYLKALG KRSSYNCEYI KEMQCKSRPC HLDNSASAQE 310 320 RLSTAWSLLL LLAFLAVGPF PGSPGLF

References

[1]

"Molecular characterization of NAD:arginine ADP-ribosyltransferase from rabbit skeletal muscle." Zolkiewska A., Nightingale M.S., Moss J. Proc. Natl. Acad. Sci. U.S.A. 89:11352-11356(1992) [PubMed: 1454819] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Skeletal muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M98764 mRNA. Translation: AAA31418.1.
PIR	A47239.
RefSeq	NP_001076139.1.
UniGene	Ocu.1934
3D structure databases
HSSP	HSSP built from PDB template 1GXY based on UniProtKB P20974.
ModBase	Search...
Genome annotation databases
GeneID	100009387.
Organism-specific databases
CTD	100009387.
Phylogenomic databases
HOVERGEN	Q03515.
Enzyme and pathway databases
BRENDA	2.4.2.31. 255.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAR1_RABIT

Accession

Primary (citable) accession number: Q03515

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	October 13, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)