Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q03503

- NAA30_YEAST

UniProt

Q03503 - NAA30_YEAST

Protein

N-alpha-acetyltransferase 30

Gene

MAK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic component of the NatC N-terminal acetyltransferase, which catalyzes acetylation of the N-terminus Met of L-A virus GAG protein and possibly GRH1.2 Publications

    Catalytic activityi

    Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

    GO - Molecular functioni

    1. peptide alpha-N-acetyltransferase activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. N-terminal protein amino acid acetylation Source: SGD
    2. regulation of protein localization Source: SGD

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YPR051W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-alpha-acetyltransferase 30 (EC:2.3.1.88)
    Alternative name(s):
    L-A virus GAG protein N-acetyltransferase subunit MAK3
    Maintenance of killer protein 3
    N-terminal acetyltransferase C complex catalytic subunit MAK3
    Short name:
    NatC complex subunit MAK3
    NatC catalytic subunit
    Gene namesi
    Name:MAK3
    Synonyms:NAA30
    Ordered Locus Names:YPR051W
    ORF Names:YP9499.08
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR051w.
    SGDiS000006255. MAK3.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. NatC complex Source: SGD
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 176176N-alpha-acetyltransferase 30PRO_0000074560Add
    BLAST

    Proteomic databases

    MaxQBiQ03503.
    PaxDbiQ03503.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03503.

    Interactioni

    Subunit structurei

    Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of MAK3, MAK10 and MAK31.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MAK10Q021975EBI-10388,EBI-10924

    Protein-protein interaction databases

    BioGridi36226. 66 interactions.
    DIPiDIP-1427N.
    IntActiQ03503. 4 interactions.
    MINTiMINT-383720.
    STRINGi4932.YPR051W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03503.
    SMRiQ03503. Positions 14-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 159157N-acetyltransferasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0456.
    GeneTreeiENSGT00390000005665.
    HOGENOMiHOG000196601.
    KOiK00670.
    OMAiVVLETEY.
    OrthoDBiEOG7P02WW.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view]
    PfamiPF00583. Acetyltransf_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    PROSITEiPS51186. GNAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03503-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEIVYKPLDI RNEEQFASIK KLIDADLSEP YSIYVYRYFL NQWPELTYIA    50
    VDNKSGTPNI PIGCIVCKMD PHRNVRLRGY IGMLAVESTY RGHGIAKKLV 100
    EIAIDKMQRE HCDEIMLETE VENSAALNLY EGMGFIRMKR MFRYYLNEGD 150
    AFKLILPLTE KSCTRSTFLM HGRLAT 176
    Length:176
    Mass (Da):20,457
    Last modified:October 1, 1993 - v1
    Checksum:iDFBF10376FE89913
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95912 Genomic RNA. Translation: AAA34753.1.
    Z49219 Genomic DNA. Translation: CAA89170.1.
    Z71255 Genomic DNA. Translation: CAA94997.1.
    BK006949 Genomic DNA. Translation: DAA11474.1.
    PIRiB44031.
    RefSeqiNP_015376.1. NM_001184148.1.

    Genome annotation databases

    EnsemblFungiiYPR051W; YPR051W; YPR051W.
    GeneIDi856163.
    KEGGisce:YPR051W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95912 Genomic RNA. Translation: AAA34753.1 .
    Z49219 Genomic DNA. Translation: CAA89170.1 .
    Z71255 Genomic DNA. Translation: CAA94997.1 .
    BK006949 Genomic DNA. Translation: DAA11474.1 .
    PIRi B44031.
    RefSeqi NP_015376.1. NM_001184148.1.

    3D structure databases

    ProteinModelPortali Q03503.
    SMRi Q03503. Positions 14-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36226. 66 interactions.
    DIPi DIP-1427N.
    IntActi Q03503. 4 interactions.
    MINTi MINT-383720.
    STRINGi 4932.YPR051W.

    Proteomic databases

    MaxQBi Q03503.
    PaxDbi Q03503.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR051W ; YPR051W ; YPR051W .
    GeneIDi 856163.
    KEGGi sce:YPR051W.

    Organism-specific databases

    CYGDi YPR051w.
    SGDi S000006255. MAK3.

    Phylogenomic databases

    eggNOGi COG0456.
    GeneTreei ENSGT00390000005665.
    HOGENOMi HOG000196601.
    KOi K00670.
    OMAi VVLETEY.
    OrthoDBi EOG7P02WW.

    Enzyme and pathway databases

    BioCyci YEAST:YPR051W-MONOMER.

    Miscellaneous databases

    NextBioi 981310.
    PROi Q03503.

    Gene expression databases

    Genevestigatori Q03503.

    Family and domain databases

    Gene3Di 3.40.630.30. 1 hit.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR000182. GNAT_dom.
    [Graphical view ]
    Pfami PF00583. Acetyltransf_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 1 hit.
    PROSITEi PS51186. GNAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MAK3 encodes an N-acetyltransferase whose modification of the L-A gag NH2 terminus is necessary for virus particle assembly."
      Tercero J.C., Wickner R.B.
      J. Biol. Chem. 267:20277-20281(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Localized mutagenesis and evidence for post-transcriptional regulation of MAK3. A putative N-acetyltransferase required for double-stranded RNA virus propagation in Saccharomyces cerevisiae."
      Tercero J.C., Riles L.E., Wickner R.B.
      J. Biol. Chem. 267:20270-20276(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    5. "NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p."
      Polevoda B., Sherman F.
      J. Biol. Chem. 276:20154-20159(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE NATC COMPLEX, FUNCTION OF THE NATC COMPLEX.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic."
      Behnia R., Barr F.A., Flanagan J.J., Barlowe C., Munro S.
      J. Cell Biol. 176:255-261(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNAA30_YEAST
    AccessioniPrimary (citable) accession number: Q03503
    Secondary accession number(s): D6W458
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1940 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3