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Reviewed, UniProtKB/Swiss-Prot Q03503 (MAK3_YEAST)

Last modified January 19, 2010. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    N-terminal acetyltransferase C complex catalytic subunit MAK3
      Short name=NatC complex subunit MAK3
    EC=2.3.1.88
Alternative name(s):
    L-A virus GAG protein N-acetyltransferase subunit MAK3
    Maintenance of killer protein 3
Gene names
Name: MAK3
Ordered Locus Names: YPR051W
ORF Names: YP9499.08
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length176 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalytic component of the NatC N-terminal acetyltransferase, which catalyzes acetylation of the N-terminus Met of L-A virus Gag protein. Ref.4

Catalytic activity

Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

Subunit structure

Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of MAK3, MAK10 and MAK31.

Subcellular location

Cytoplasm. Nucleus Ref.5.

Miscellaneous

Present with 1940 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the acetyltransferase family. MAK3 subfamily.

Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processN-terminal protein amino acid acetylation Ref.4

Inferred from mutant phenotype. Source: SGD

   Cellular componentNatC complex

Inferred from direct assay. Source: SGD

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionpeptide alpha-N-acetyltransferase activity Ref.4

Inferred from mutant phenotype. Source: SGD

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MAK10Q021973EBI-10388,EBI-10924

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 176176N-terminal acetyltransferase C complex catalytic subunit MAK3
PRO_0000074560

Regions

Domain3 – 159157N-acetyltransferase

Sequences

Sequence LengthMass (Da)Tools
Q03503-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: DFBF10376FE89913

FASTA17620,457
        10         20         30         40         50         60 
MEIVYKPLDI RNEEQFASIK KLIDADLSEP YSIYVYRYFL NQWPELTYIA VDNKSGTPNI 

        70         80         90        100        110        120 
PIGCIVCKMD PHRNVRLRGY IGMLAVESTY RGHGIAKKLV EIAIDKMQRE HCDEIMLETE 

       130        140        150        160        170 
VENSAALNLY EGMGFIRMKR MFRYYLNEGD AFKLILPLTE KSCTRSTFLM HGRLAT 

« Hide

References

« Hide 'large scale' references
[1]"MAK3 encodes an N-acetyltransferase whose modification of the L-A gag NH2 terminus is necessary for virus particle assembly."
Tercero J.C., Wickner R.B.
J. Biol. Chem. 267:20277-20281(1992) [PubMed: 1400344] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed: 9169875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Localized mutagenesis and evidence for post-transcriptional regulation of MAK3. A putative N-acetyltransferase required for double-stranded RNA virus propagation in Saccharomyces cerevisiae."
Tercero J.C., Riles L.E., Wickner R.B.
J. Biol. Chem. 267:20270-20276(1992) [PubMed: 1339437] [Abstract]
Cited for: MUTAGENESIS.
[4]"NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p."
Polevoda B., Sherman F.
J. Biol. Chem. 276:20154-20159(2001) [PubMed: 11274203] [Abstract]
Cited for: IDENTIFICATION IN THE NATC COMPLEX, FUNCTION OF THE NATC COMPLEX.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95912 Genomic RNA. Translation: AAA34753.1.
Z49219 Genomic DNA. Translation: CAA89170.1.
Z71255 Genomic DNA. Translation: CAA94997.1.
PIRB44031.
RefSeqNP_015376.1.

3D structure databases

SMRQ03503. Positions 13-147.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1427N.
IntActQ03503. 5 interactions.
STRINGQ03503.

Genome annotation databases

EnsemblYPR051W; YPR051W; YPR051W; Saccharomyces cerevisiae. [Genome view]
GeneID856163.
KEGGsce:YPR051W.
NMPDRfig|4932.3.peg.6512.

Organism-specific databases

CYGDYPR051w.
SGDS000006255. MAK3.

Phylogenomic databases

eggNOGfuNOG09079.
HOGENOMHBG742014.
OMADEIMLET.
OrthoDBEOG9SXPVW.
PhylomeDBQ03503.

Enzyme and pathway databases

BRENDA2.3.1.88. 250.

Gene expression databases

ArrayExpressQ03503.
GenevestigatorQ03503.
GermOnlineYPR051W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GCN5-rel_AcTrfase.
[Graphical view]
Gene3DG3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
PfamPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PROSITEPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio981310.

Entry information

Entry nameMAK3_YEAST
AccessionPrimary (citable) accession number: Q03503
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: January 19, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents