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Protein

N-alpha-acetyltransferase 30

Gene

MAK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the NatC N-terminal acetyltransferase, which catalyzes acetylation of the N-terminus Met of L-A virus GAG protein and possibly GRH1.2 Publications

Catalytic activityi

Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

GO - Molecular functioni

  1. peptide alpha-N-acetyltransferase activity Source: SGD

GO - Biological processi

  1. N-terminal protein amino acid acetylation Source: SGD
  2. regulation of protein localization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YPR051W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-alpha-acetyltransferase 30 (EC:2.3.1.88)
Alternative name(s):
L-A virus GAG protein N-acetyltransferase subunit MAK3
Maintenance of killer protein 3
N-terminal acetyltransferase C complex catalytic subunit MAK3
Short name:
NatC complex subunit MAK3
NatC catalytic subunit
Gene namesi
Name:MAK3
Synonyms:NAA30
Ordered Locus Names:YPR051W
ORF Names:YP9499.08
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPR051w.
SGDiS000006255. MAK3.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. NatC complex Source: SGD
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 176176N-alpha-acetyltransferase 30PRO_0000074560Add
BLAST

Proteomic databases

MaxQBiQ03503.
PaxDbiQ03503.

Expressioni

Gene expression databases

GenevestigatoriQ03503.

Interactioni

Subunit structurei

Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of MAK3, MAK10 and MAK31.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MAK10Q021975EBI-10388,EBI-10924

Protein-protein interaction databases

BioGridi36226. 68 interactions.
DIPiDIP-1427N.
IntActiQ03503. 4 interactions.
MINTiMINT-383720.
STRINGi4932.YPR051W.

Structurei

3D structure databases

ProteinModelPortaliQ03503.
SMRiQ03503. Positions 14-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 159157N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0456.
GeneTreeiENSGT00390000005665.
HOGENOMiHOG000196601.
InParanoidiQ03503.
KOiK00670.
OMAiKNWRKRG.
OrthoDBiEOG7P02WW.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIVYKPLDI RNEEQFASIK KLIDADLSEP YSIYVYRYFL NQWPELTYIA
60 70 80 90 100
VDNKSGTPNI PIGCIVCKMD PHRNVRLRGY IGMLAVESTY RGHGIAKKLV
110 120 130 140 150
EIAIDKMQRE HCDEIMLETE VENSAALNLY EGMGFIRMKR MFRYYLNEGD
160 170
AFKLILPLTE KSCTRSTFLM HGRLAT
Length:176
Mass (Da):20,457
Last modified:October 1, 1993 - v1
Checksum:iDFBF10376FE89913
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95912 Genomic RNA. Translation: AAA34753.1.
Z49219 Genomic DNA. Translation: CAA89170.1.
Z71255 Genomic DNA. Translation: CAA94997.1.
BK006949 Genomic DNA. Translation: DAA11474.1.
PIRiB44031.
RefSeqiNP_015376.1. NM_001184148.1.

Genome annotation databases

EnsemblFungiiYPR051W; YPR051W; YPR051W.
GeneIDi856163.
KEGGisce:YPR051W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95912 Genomic RNA. Translation: AAA34753.1.
Z49219 Genomic DNA. Translation: CAA89170.1.
Z71255 Genomic DNA. Translation: CAA94997.1.
BK006949 Genomic DNA. Translation: DAA11474.1.
PIRiB44031.
RefSeqiNP_015376.1. NM_001184148.1.

3D structure databases

ProteinModelPortaliQ03503.
SMRiQ03503. Positions 14-157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36226. 68 interactions.
DIPiDIP-1427N.
IntActiQ03503. 4 interactions.
MINTiMINT-383720.
STRINGi4932.YPR051W.

Proteomic databases

MaxQBiQ03503.
PaxDbiQ03503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR051W; YPR051W; YPR051W.
GeneIDi856163.
KEGGisce:YPR051W.

Organism-specific databases

CYGDiYPR051w.
SGDiS000006255. MAK3.

Phylogenomic databases

eggNOGiCOG0456.
GeneTreeiENSGT00390000005665.
HOGENOMiHOG000196601.
InParanoidiQ03503.
KOiK00670.
OMAiKNWRKRG.
OrthoDBiEOG7P02WW.

Enzyme and pathway databases

BioCyciYEAST:YPR051W-MONOMER.

Miscellaneous databases

NextBioi981310.
PROiQ03503.

Gene expression databases

GenevestigatoriQ03503.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MAK3 encodes an N-acetyltransferase whose modification of the L-A gag NH2 terminus is necessary for virus particle assembly."
    Tercero J.C., Wickner R.B.
    J. Biol. Chem. 267:20277-20281(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Localized mutagenesis and evidence for post-transcriptional regulation of MAK3. A putative N-acetyltransferase required for double-stranded RNA virus propagation in Saccharomyces cerevisiae."
    Tercero J.C., Riles L.E., Wickner R.B.
    J. Biol. Chem. 267:20270-20276(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  5. "NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p."
    Polevoda B., Sherman F.
    J. Biol. Chem. 276:20154-20159(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NATC COMPLEX, FUNCTION OF THE NATC COMPLEX.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic."
    Behnia R., Barr F.A., Flanagan J.J., Barlowe C., Munro S.
    J. Cell Biol. 176:255-261(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNAA30_YEAST
AccessioniPrimary (citable) accession number: Q03503
Secondary accession number(s): D6W458
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 4, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1940 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.