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Q03503 (NAA30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-alpha-acetyltransferase 30

EC=2.3.1.88
Alternative name(s):
L-A virus GAG protein N-acetyltransferase subunit MAK3
Maintenance of killer protein 3
N-terminal acetyltransferase C complex catalytic subunit MAK3
Short name=NatC complex subunit MAK3
NatC catalytic subunit
Gene names
Name:MAK3
Synonyms:NAA30
Ordered Locus Names:YPR051W
ORF Names:YP9499.08
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length176 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic component of the NatC N-terminal acetyltransferase, which catalyzes acetylation of the N-terminus Met of L-A virus GAG protein and possibly GRH1. Ref.5 Ref.8

Catalytic activity

Acetyl-CoA + peptide = N(alpha)-acetylpeptide + CoA.

Subunit structure

Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of MAK3, MAK10 and MAK31. Ref.5

Subcellular location

Cytoplasm. Nucleus Ref.6.

Miscellaneous

Present with 1940 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the acetyltransferase family. MAK3 subfamily.

Contains 1 N-acetyltransferase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MAK10Q021975EBI-10388,EBI-10924

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 176176N-alpha-acetyltransferase 30
PRO_0000074560

Regions

Domain3 – 159157N-acetyltransferase

Sequences

Sequence LengthMass (Da)Tools
Q03503 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: DFBF10376FE89913

FASTA17620,457
        10         20         30         40         50         60 
MEIVYKPLDI RNEEQFASIK KLIDADLSEP YSIYVYRYFL NQWPELTYIA VDNKSGTPNI 

        70         80         90        100        110        120 
PIGCIVCKMD PHRNVRLRGY IGMLAVESTY RGHGIAKKLV EIAIDKMQRE HCDEIMLETE 

       130        140        150        160        170 
VENSAALNLY EGMGFIRMKR MFRYYLNEGD AFKLILPLTE KSCTRSTFLM HGRLAT 

« Hide

References

« Hide 'large scale' references
[1]"MAK3 encodes an N-acetyltransferase whose modification of the L-A gag NH2 terminus is necessary for virus particle assembly."
Tercero J.C., Wickner R.B.
J. Biol. Chem. 267:20277-20281(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Localized mutagenesis and evidence for post-transcriptional regulation of MAK3. A putative N-acetyltransferase required for double-stranded RNA virus propagation in Saccharomyces cerevisiae."
Tercero J.C., Riles L.E., Wickner R.B.
J. Biol. Chem. 267:20270-20276(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[5]"NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p."
Polevoda B., Sherman F.
J. Biol. Chem. 276:20154-20159(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE NATC COMPLEX, FUNCTION OF THE NATC COMPLEX.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic."
Behnia R., Barr F.A., Flanagan J.J., Barlowe C., Munro S.
J. Cell Biol. 176:255-261(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95912 Genomic RNA. Translation: AAA34753.1.
Z49219 Genomic DNA. Translation: CAA89170.1.
Z71255 Genomic DNA. Translation: CAA94997.1.
BK006949 Genomic DNA. Translation: DAA11474.1.
PIRB44031.
RefSeqNP_015376.1. NM_001184148.1.

3D structure databases

ProteinModelPortalQ03503.
SMRQ03503. Positions 14-157.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36226. 66 interactions.
DIPDIP-1427N.
IntActQ03503. 4 interactions.
MINTMINT-383720.
STRING4932.YPR051W.

Proteomic databases

PaxDbQ03503.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR051W; YPR051W; YPR051W.
GeneID856163.
KEGGsce:YPR051W.

Organism-specific databases

CYGDYPR051w.
SGDS000006255. MAK3.

Phylogenomic databases

eggNOGCOG0456.
GeneTreeENSGT00390000005665.
HOGENOMHOG000196601.
KOK00670.
OMAVVLETEY.
OrthoDBEOG7P02WW.

Enzyme and pathway databases

BioCycYEAST:YPR051W-MONOMER.

Gene expression databases

GenevestigatorQ03503.

Family and domain databases

Gene3D3.40.630.30. 1 hit.
InterProIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMSSF55729. SSF55729. 1 hit.
PROSITEPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981310.
PROQ03503.

Entry information

Entry nameNAA30_YEAST
AccessionPrimary (citable) accession number: Q03503
Secondary accession number(s): D6W458
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 19, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families