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Protein

6-phospho-alpha-glucosidase 2

Gene

LSEI_2684

Organism
Lactobacillus casei (strain ATCC 334)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarityNote: Binds 1 divalent metal ion per subunit. Mn2+.By similarity
  • NAD(+)By similarityNote: Binds 1 NAD(+) per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961SubstrateBy similarity
Sitei112 – 1121Increases basicity of active site TyrBy similarity
Binding sitei150 – 1501SubstrateBy similarity
Metal bindingi172 – 1721ManganeseBy similarity
Active sitei173 – 1731Proton donorBy similarity
Metal bindingi202 – 2021ManganeseBy similarity
Active sitei267 – 2671Proton acceptorBy similarity
Binding sitei287 – 2871SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 7367NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciLCAS321967:GH4S-2681-MONOMER.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-alpha-glucosidase 2 (EC:3.2.1.122)
Gene namesi
Ordered Locus Names:LSEI_2684
OrganismiLactobacillus casei (strain ATCC 334)
Taxonomic identifieri321967 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001651 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4614616-phospho-alpha-glucosidase 2PRO_0000389205Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ034G9.
SMRiQ034G9. Positions 5-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

eggNOGiCOG1486.
HOGENOMiHOG000239810.
KOiK01232.
OMAiLHRFPIR.
OrthoDBiEOG632D2X.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q034G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQNKQFSIV IAGGGSTYTA GIVMMLLENA ERFPLRALKL YDIDEERQAT
60 70 80 90 100
IAEAIAIELK EKAPAIDFTW TTDPQTAFTD VDFCFAHIRS GGYKMREQDE
110 120 130 140 150
KIPLKHHVVG QETCGPGGIA YGMRSIGDII ELIDFIEKYS PDCWMLNYSN
160 170 180 190 200
PASIVAEACR RLRPDAKILN ICDMPVGTQR RMSQIIGLQP KDLEVRYFGM
210 220 230 240 250
NHFGWWTSVK DKAGNEYLPQ IRDYVAHHGY LTQIEVDTQH MDASWQATHK
260 270 280 290 300
KAQDLLAVDP HYLPNTYLKY YLYPDYVVAH SDPDYTRANE VEDGREKCVF
310 320 330 340 350
SAAQKIIDAG TSDVGSFPID SHASFIVDLA CAIAFNTHER MLLIVENNGA
360 370 380 390 400
VANIDDNIMV EVPCIVGKDG AEPLTQGKIP MFQRGMILNQ AMVEKLVVDA
410 420 430 440 450
WINHDYQELW QALTLSKTLP SAQVAKEVLD DLIEANREYW PELKHDEHEV
460
HLISESAVSE I
Length:461
Mass (Da):52,030
Last modified:November 14, 2006 - v1
Checksum:iF96D99955DA75E84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000423 Genomic DNA. Translation: ABJ71403.1.
RefSeqiWP_011674973.1. NC_008526.1.
YP_807845.1. NC_008526.1.

Genome annotation databases

EnsemblBacteriaiABJ71403; ABJ71403; LSEI_2684.
GeneIDi4421236.
KEGGilca:LSEI_2684.
PATRICi22209078. VBILacCas62221_2628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000423 Genomic DNA. Translation: ABJ71403.1.
RefSeqiWP_011674973.1. NC_008526.1.
YP_807845.1. NC_008526.1.

3D structure databases

ProteinModelPortaliQ034G9.
SMRiQ034G9. Positions 5-443.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ71403; ABJ71403; LSEI_2684.
GeneIDi4421236.
KEGGilca:LSEI_2684.
PATRICi22209078. VBILacCas62221_2628.

Phylogenomic databases

eggNOGiCOG1486.
HOGENOMiHOG000239810.
KOiK01232.
OMAiLHRFPIR.
OrthoDBiEOG632D2X.

Enzyme and pathway databases

BioCyciLCAS321967:GH4S-2681-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 334.
  2. "The sim operon facilitates the transport and metabolism of sucrose isomers in Lactobacillus casei ATCC 334."
    Thompson J., Jakubovics N., Abraham B., Hess S., Pikis A.
    J. Bacteriol. 190:3362-3373(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiPAGL2_LACC3
AccessioniPrimary (citable) accession number: Q034G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: November 14, 2006
Last modified: June 24, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.