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Q03497

- STE20_YEAST

UniProt

Q03497 - STE20_YEAST

Protein

Serine/threonine-protein kinase STE20

Gene

STE20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 161 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H2O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling.20 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei649 – 6491ATPPROSITE-ProRule annotation
    Active sitei739 – 7391Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi626 – 6349ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. histone kinase activity (H2B-S14 specific) Source: SGD
    3. identical protein binding Source: IntAct
    4. MAP kinase kinase kinase kinase activity Source: SGD
    5. protein binding Source: IntAct

    GO - Biological processi

    1. activation of MAPKKK activity Source: SGD
    2. bipolar cellular bud site selection Source: SGD
    3. budding cell apical bud growth Source: SGD
    4. cellular response to heat Source: SGD
    5. histone H2B-S14 phosphorylation Source: GOC
    6. invasive growth in response to glucose limitation Source: SGD
    7. MAPK cascade Source: GOC
    8. negative regulation of gene expression Source: SGD
    9. osmosensory signaling pathway via Sho1 osmosensor Source: SGD
    10. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
    11. positive regulation of apoptotic process Source: SGD
    12. protein phosphorylation Source: SGD
    13. pseudohyphal growth Source: SGD
    14. regulation of exit from mitosis Source: SGD
    15. signal transduction involved in filamentous growth Source: SGD
    16. sterol import Source: SGD
    17. stress granule assembly Source: SGD
    18. vacuole inheritance Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Pheromone response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31029-MONOMER.
    BRENDAi2.7.11.1. 984.
    ReactomeiREACT_100522. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_189214. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase STE20 (EC:2.7.11.1)
    Gene namesi
    Name:STE20
    Ordered Locus Names:YHL007C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHL007c.
    SGDiS000000999. STE20.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: The translocation from the cytoplasm to the nucleus is stimulated by H2O2. Localizes at bud emergence during cell cycle and the shmoo top during mating, both localizations requiring an interaction with CDC42.

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. incipient cellular bud site Source: SGD
    3. mating projection tip Source: SGD
    4. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi338 – 3381S → A: Reduces interaction with CDC42. 1 Publication
    Mutagenesisi345 – 3451H → A or D: Reduces interaction with CDC42. 2 Publications
    Mutagenesisi348 – 3481H → D: Reduces interaction with CDC42. 1 Publication
    Mutagenesisi475 – 4751P → G: Impairs interaction with BEM1; when associated with A-477. 1 Publication
    Mutagenesisi477 – 4771P → A: Impairs interaction with BEM1; when associated with G-475. 1 Publication
    Mutagenesisi649 – 6491K → R: Impairs phosphorylation of STE11 and histone H2B and mating efficiency. 2 Publications
    Mutagenesisi777 – 7771T → A: Impairs autophosphorylation and mating efficiency. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 939938Serine/threonine-protein kinase STE20PRO_0000086686Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei87 – 871Phosphoserine2 Publications
    Modified residuei165 – 1651Phosphoserine2 Publications
    Modified residuei167 – 1671Phosphothreonine2 Publications
    Modified residuei169 – 1691Phosphoserine4 Publications
    Modified residuei203 – 2031Phosphothreonine4 Publications
    Modified residuei418 – 4181Phosphoserine2 Publications
    Modified residuei502 – 5021Phosphoserine3 Publications
    Modified residuei547 – 5471Phosphoserine3 Publications
    Modified residuei562 – 5621Phosphoserine5 Publications
    Modified residuei573 – 5731Phosphothreonine2 Publications
    Modified residuei585 – 5851Phosphoserine4 Publications
    Modified residuei773 – 7731Phosphothreonine2 Publications
    Modified residuei924 – 9241Phosphoserine2 Publications
    Modified residuei927 – 9271Phosphothreonine2 Publications

    Post-translational modificationi

    Autophosphorylated and phosphorylated by the CLN2-CDC28 complex in a cell cycle dependent manner.
    Autophosphorylated on serine residues.

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ03497.
    PaxDbiQ03497.
    PeptideAtlasiQ03497.
    PRIDEiQ03497.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03497.

    Interactioni

    Subunit structurei

    Interacts with BEM1, CDC42, CLN2, STE4 and the 14-3-3 proteins BMH1 and BMH2.11 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-18285,EBI-18285
    BEM1P2936611EBI-18285,EBI-3508
    CDC42P190734EBI-18285,EBI-4274
    MYO3P360063EBI-18285,EBI-11670
    MYO5Q044394EBI-18285,EBI-11687
    NBP2Q121635EBI-18285,EBI-34713
    PEX13P806673EBI-18285,EBI-13206
    STE4P188513EBI-18285,EBI-7390

    Protein-protein interaction databases

    BioGridi36419. 234 interactions.
    DIPiDIP-712N.
    IntActiQ03497. 23 interactions.
    MINTiMINT-582880.
    STRINGi4932.YHL007C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KYMNMR-B468-483[»]
    2LCSNMR-B468-483[»]
    2RQWNMR-B463-486[»]
    ProteinModelPortaliQ03497.
    SMRiQ03497. Positions 337-380, 560-930.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ03497.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini337 – 35014CRIBPROSITE-ProRule annotationAdd
    BLAST
    Domaini620 – 871252Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni434 – 49966BEM1-bindingAdd
    BLAST

    Domaini

    The CRIB domain is required for the association with CDC42.

    Sequence similaritiesi

    Contains 1 CRIB domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00670000097939.
    HOGENOMiHOG000234202.
    KOiK04409.
    OMAiTDVVTHC.
    OrthoDBiEOG708W7W.

    Family and domain databases

    Gene3Di3.90.810.10. 1 hit.
    InterProiIPR000095. CRIB_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00786. PBD. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00285. PBD. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50108. CRIB. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03497-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNDPSAVSE LPDKDSLDNG ISNDNERAMG GNGDGGDGLR LPRTTGTLNV    50
    NALQKGTNAA HEAGGYKSMD PAKNAETTND DDNNVVSLDD PIQFTRVSSS 100
    SVISGMSSSM SPHSNIDETK SLEAVTPNIN TSNITPDHSA DNTFSTINAS 150
    ESDHQFNDTL LSKLSLTDST ETIENNATVK HQQPVASSTV NSNKSSTDIR 200
    RATPVSTPVI SKPSMTTTPR QINSASHSLS NPKHKQHKPK VKPSKPEAKS 250
    KPVSVKKSFP SKNPLKNSSP PKKQTEKSYY SSSSKKRKSG SNSGTLRMKD 300
    VFTSFVQNIK RNSQDDKRAS SSSNNSSSSS ITTALRISTP YNAKHIHHVG 350
    VDSKTGEYTG LPEEWEKLLT SSGISKREQQ QNMQAVMDIV KFYQDVTETN 400
    GEDKMFKTFN TTTGLPGSPQ VSTPPANSFN KFPPSTSDSH NYGSRTGTPM 450
    SNHVMSPTLN TDSSSANGKF IPSRPAPKPP SSASASAPII KSPVMNSAAN 500
    VSPLKQTHAP TTPNRTSPNR SSISRNATLK KEEQPLPPIP PTKSKTSPII 550
    STAHTPQQVA QSPKAPAQET VTTPTSKPAQ ARSLSKELNE KKREERERRK 600
    KQLYAKLNEI CSDGDPSTKY ANLVKIGQGA SGGVYTAYEI GTNVSVAIKQ 650
    MNLEKQPKKE LIINEILVMK GSKHPNIVNF IDSYVLKGDL WVIMEYMEGG 700
    SLTDVVTHCI LTEGQIGAVC RETLSGLEFL HSKGVLHRDI KSDNILLSME 750
    GDIKLTDFGF CAQINELNLK RTTMVGTPYW MAPEVVSRKE YGPKVDIWSL 800
    GIMIIEMIEG EPPYLNETPL RALYLIATNG TPKLKEPENL SSSLKKFLDW 850
    CLCVEPEDRA SATELLHDEY ITEIAEANSS LAPLVKLARL KKVAENMDAD 900
    EDNDDDNDNE HINKTNNCDD NNDSKETVNL DVTEDDKQK 939
    Length:939
    Mass (Da):102,362
    Last modified:October 1, 1993 - v1
    Checksum:i69C1C12F5B87733C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191N → S in AAA35038. (PubMed:8421676)Curated
    Sequence conflicti134 – 1341I → M in AAA35038. (PubMed:8421676)Curated
    Sequence conflicti271 – 2711P → S in AAA35038. (PubMed:8421676)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94719 Genomic DNA. Translation: AAA35111.1.
    L04655 Genomic DNA. Translation: AAA35038.1.
    L04655 Genomic DNA. Translation: AAA35039.1.
    U11581 Genomic DNA. Translation: AAB69747.1.
    BK006934 Genomic DNA. Translation: DAA06681.1.
    PIRiS28394.
    RefSeqiNP_011856.1. NM_001179087.1.

    Genome annotation databases

    EnsemblFungiiYHL007C; YHL007C; YHL007C.
    GeneIDi856382.
    KEGGisce:YHL007C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94719 Genomic DNA. Translation: AAA35111.1 .
    L04655 Genomic DNA. Translation: AAA35038.1 .
    L04655 Genomic DNA. Translation: AAA35039.1 .
    U11581 Genomic DNA. Translation: AAB69747.1 .
    BK006934 Genomic DNA. Translation: DAA06681.1 .
    PIRi S28394.
    RefSeqi NP_011856.1. NM_001179087.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KYM NMR - B 468-483 [» ]
    2LCS NMR - B 468-483 [» ]
    2RQW NMR - B 463-486 [» ]
    ProteinModelPortali Q03497.
    SMRi Q03497. Positions 337-380, 560-930.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36419. 234 interactions.
    DIPi DIP-712N.
    IntActi Q03497. 23 interactions.
    MINTi MINT-582880.
    STRINGi 4932.YHL007C.

    Proteomic databases

    MaxQBi Q03497.
    PaxDbi Q03497.
    PeptideAtlasi Q03497.
    PRIDEi Q03497.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHL007C ; YHL007C ; YHL007C .
    GeneIDi 856382.
    KEGGi sce:YHL007C.

    Organism-specific databases

    CYGDi YHL007c.
    SGDi S000000999. STE20.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00670000097939.
    HOGENOMi HOG000234202.
    KOi K04409.
    OMAi TDVVTHC.
    OrthoDBi EOG708W7W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31029-MONOMER.
    BRENDAi 2.7.11.1. 984.
    Reactomei REACT_100522. Regulation of activated PAK-2p34 by proteasome mediated degradation.
    REACT_189214. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    EvolutionaryTracei Q03497.
    NextBioi 981881.
    PROi Q03497.

    Gene expression databases

    Genevestigatori Q03497.

    Family and domain databases

    Gene3Di 3.90.810.10. 1 hit.
    InterProi IPR000095. CRIB_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00786. PBD. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00285. PBD. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50108. CRIB. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components."
      Leberer E., Dignard D., Harcus D., Thomas D.Y., Whiteway M.
      EMBO J. 11:4815-4824(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae."
      Ramer S.W., Davis R.W.
      Proc. Natl. Acad. Sci. U.S.A. 90:452-456(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The RA domain of Ste50 adaptor protein is required for delivery of Ste11 to the plasma membrane in the filamentous growth signaling pathway of the yeast Saccharomyces cerevisiae."
      Truckses D.M., Bloomekatz J.E., Thorner J.
      Mol. Cell. Biol. 26:912-928(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 164-169; 583-591 AND 408-431, PHOSPHORYLATION AT SER-169; SER-585 AND SER-418.
    6. "Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae."
      Wu C., Whiteway M., Thomas D.Y., Leberer E.
      J. Biol. Chem. 270:15984-15992(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: AUTOPHOSPHORYLATION, FUNCTION IN PHOSPHORYLATION OF STE11, MUTAGENESIS OF LYS-649 AND THR-777.
    7. "Functional analysis of the interaction between the small GTP binding protein Cdc42 and the Ste20 protein kinase in yeast."
      Peter M., Neiman A.M., Park H.-O., van Lohuizen M., Herskowitz I.
      EMBO J. 15:7046-7059(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC42, SUBCELLULAR LOCATION.
    8. "Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway."
      Akada R., Kallal L., Johnson D.I., Kurjan J.
      Genetics 143:103-117(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Ras2 signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae."
      Moesch H.-U., Roberts R.L., Fink G.R.
      Proc. Natl. Acad. Sci. U.S.A. 93:5352-5356(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "14-3-3 proteins are essential for RAS/MAPK cascade signaling during pseudohyphal development in S. cerevisiae."
      Roberts R.L., Moesch H.-U., Fink G.R.
      Cell 89:1055-1065(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BMH1 AND BMH2.
    11. "Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase."
      Leberer E., Wu C., Leeuw T., Fourest-Lieuvin A., Segall J.E., Thomas D.Y.
      EMBO J. 16:83-97(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC42, SUBCELLULAR LOCATION.
    12. "The phosphorylation site for Ste20p-like protein kinases is essential for the function of myosin-I in yeast."
      Wu C., Lytvyn V., Thomas D.Y., Leberer E.
      J. Biol. Chem. 272:30623-30626(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF MYO3.
    13. "Actin cytoskeleton organization regulated by the PAK family of protein kinases."
      Eby J.J., Holly S.P., van Drogen F., Grishin A.V., Peter M., Drubin D.G., Blumer K.J.
      Curr. Biol. 8:967-970(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Cell cycle- and Cln2p-Cdc28p-dependent phosphorylation of the yeast Ste20p protein kinase."
      Wu C., Leeuw T., Leberer E., Thomas D.Y., Whiteway M.
      J. Biol. Chem. 273:28107-28115(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY THE CLN2-CDC28 COMPLEX.
    15. "Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases."
      Leeuw T., Wu C., Schrag J.D., Whiteway M., Thomas D.Y., Leberer E.
      Nature 391:191-195(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH STE4.
    16. "PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae."
      Holly S.P., Blumer K.J.
      J. Cell Biol. 147:845-856(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    17. "Accurate quantitation of protein expression and site-specific phosphorylation."
      Oda Y., Huang K., Cross F.R., Cowburn D., Chait B.T.
      Proc. Natl. Acad. Sci. U.S.A. 96:6591-6596(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLN2, IDENTIFICATION BY MASS SPECTROMETRY, PHOSPHORYLATION AT SER-547; SER-562; THR-573; SER-585 AND THR-773.
    18. "Phosphorylation of the MEKK Ste11p by the PAK-like kinase Ste20p is required for MAP kinase signaling in vivo."
      van Drogen F., O'Rourke S.M., Stucke V.M., Jaquenoud M., Neiman A.M., Peter M.
      Curr. Biol. 10:630-639(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF STE11.
    19. "Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway."
      Raitt D.C., Posas F., Saito H.
      EMBO J. 19:4623-4631(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    20. "Role of Cdc42p in pheromone-stimulated signal transduction in Saccharomyces cerevisiae."
      Moskow J.J., Gladfelter A.S., Lamson R.E., Pryciak P.M., Lew D.J.
      Mol. Cell. Biol. 20:7559-7571(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC42, SUBCELLULAR LOCATION.
    21. "Saccharomyces cerevisiae cdc42p GTPase is involved in preventing the recurrence of bud emergence during the cell cycle."
      Richman T.J., Johnson D.I.
      Mol. Cell. Biol. 20:8548-8559(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC42.
    22. Cited for: INTERACTION WITH CDC42.
    23. "Cdc42 regulation of kinase activity and signaling by the yeast p21-activated kinase Ste20."
      Lamson R.E., Winters M.J., Pryciak P.M.
      Mol. Cell. Biol. 22:2939-2951(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC42, MUTAGENESIS OF SER-338 AND HIS-345.
    24. "Genetic analysis of the interface between Cdc42p and the CRIB domain of Ste20p in Saccharomyces cerevisiae."
      Ash J., Wu C., Larocque R., Jamal M., Stevens W., Osborne M., Thomas D.Y., Whiteway M.
      Genetics 163:9-20(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC42, MUTAGENESIS OF HIS-345 AND HIS-348, SUBCELLULAR LOCATION.
    25. "Synthetic lethal analysis implicates Ste20p, a p21-activated protein kinase, in polarisome activation."
      Goehring A.S., Mitchell D.A., Tong A.H., Keniry M.E., Boone C., Sprague G.F. Jr.
      Mol. Biol. Cell 14:1501-1516(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    26. "Identification of p21-activated kinase specificity determinants in budding yeast: a single amino acid substitution imparts Ste20 specificity to Cla4."
      Keniry M.E., Sprague G.F. Jr.
      Mol. Cell. Biol. 23:1569-1580(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    27. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    28. "Sterile 20 kinase phosphorylates histone H2B at serine 10 during hydrogen peroxide-induced apoptosis in S. cerevisiae."
      Ahn S.-H., Cheung W.L., Hsu J.-Y., Diaz R.L., Smith M.M., Allis C.D.
      Cell 120:25-36(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF HISTONE H2B, MUTAGENESIS OF LYS-649, SUBCELLULAR LOCATION.
    29. "H2B (Ser10) phosphorylation is induced during apoptosis and meiosis in S. cerevisiae."
      Ahn S.-H., Henderson K.A., Keeney S., Allis C.D.
      Cell Cycle 4:780-783(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF HISTONE H2B.
    30. "Interaction with the SH3 domain protein Bem1 regulates signaling by the Saccharomyces cerevisiae p21-activated kinase Ste20."
      Winters M.J., Pryciak P.M.
      Mol. Cell. Biol. 25:2177-2190(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BEM1, MUTAGENESIS OF PRO-475 AND PRO-477, SUBCELLULAR LOCATION.
    31. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    32. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169; THR-203; SER-562 AND SER-585, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    33. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    34. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; THR-167; THR-203; SER-502; SER-924 AND THR-927, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    35. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87; SER-169; THR-203; SER-502; SER-547 AND SER-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    36. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSTE20_YEAST
    AccessioniPrimary (citable) accession number: Q03497
    Secondary accession number(s): D3DKQ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 161 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 259 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3