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Protein

Myosin IE heavy chain

Gene

myoE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Myosin is a protein that binds to actin and has ATPase activity that is activated by actin. May play a role in moving membranes relative to actin.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi101 – 108ATP8

GO - Molecular functioni

  • actin binding Source: dictyBase
  • actin-dependent ATPase activity Source: dictyBase
  • ATP binding Source: dictyBase
  • calmodulin binding Source: dictyBase
  • microfilament motor activity Source: dictyBase
  • microtubule binding Source: InterPro
  • microtubule motor activity Source: InterPro
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: dictyBase

GO - Biological processi

  • actin filament polymerization Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • microtubule-based movement Source: InterPro
  • phagocytosis Source: dictyBase
  • phagocytosis, engulfment Source: dictyBase

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, Motor protein, Myosin
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin IE heavy chain
Gene namesi
Name:myoE
Synonyms:dmiE
ORF Names:DDB_G0288679
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288679 myoE

Subcellular locationi

GO - Cellular componenti

  • cell-cell contact zone Source: dictyBase
  • cell leading edge Source: dictyBase
  • cytosol Source: dictyBase
  • early phagosome Source: dictyBase
  • leading edge membrane Source: dictyBase
  • macropinocytic cup Source: dictyBase
  • macropinocytic cup membrane Source: dictyBase
  • myosin I complex Source: dictyBase
  • phagocytic cup Source: dictyBase
  • plasma membrane Source: dictyBase
  • pseudopodium Source: dictyBase
  • pseudopodium membrane Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001233691 – 1005Myosin IE heavy chainAdd BLAST1005

Proteomic databases

PaxDbiQ03479
PRIDEiQ03479

Interactioni

Subunit structurei

Myosin I heavy chain is single-headed. Dimer of a heavy and a light chain. Inability to self-assemble into filaments.

GO - Molecular functioni

  • actin binding Source: dictyBase
  • calmodulin binding Source: dictyBase
  • microtubule binding Source: InterPro

Protein-protein interaction databases

STRINGi44689.DDB0216200

Structurei

Secondary structure

11005
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 15Combined sources3
Helixi21 – 33Combined sources13
Beta strandi38 – 43Combined sources6
Beta strandi45 – 48Combined sources4
Helixi59 – 65Combined sources7
Helixi70 – 72Combined sources3
Helixi77 – 91Combined sources15
Beta strandi95 – 100Combined sources6
Helixi107 – 121Combined sources15
Helixi127 – 147Combined sources21
Beta strandi160 – 168Combined sources9
Beta strandi174 – 182Combined sources9
Helixi186 – 189Combined sources4
Helixi200 – 206Combined sources7
Helixi211 – 217Combined sources7
Helixi223 – 225Combined sources3
Helixi227 – 230Combined sources4
Turni231 – 233Combined sources3
Helixi242 – 255Combined sources14
Helixi260 – 276Combined sources17
Beta strandi281 – 284Combined sources4
Turni288 – 290Combined sources3
Beta strandi293 – 298Combined sources6
Helixi299 – 309Combined sources11
Helixi313 – 321Combined sources9
Helixi342 – 372Combined sources31
Beta strandi381 – 386Combined sources6
Beta strandi394 – 396Combined sources3
Helixi398 – 418Combined sources21
Helixi420 – 428Combined sources9
Helixi443 – 447Combined sources5
Beta strandi450 – 454Combined sources5
Helixi455 – 464Combined sources10
Helixi470 – 480Combined sources11
Beta strandi481 – 484Combined sources4
Turni490 – 492Combined sources3
Beta strandi502 – 507Combined sources6
Beta strandi510 – 515Combined sources6
Helixi519 – 524Combined sources6
Helixi529 – 536Combined sources8
Helixi541 – 546Combined sources6
Helixi562 – 577Combined sources16
Beta strandi580 – 588Combined sources9
Helixi601 – 610Combined sources10
Helixi614 – 622Combined sources9
Helixi631 – 634Combined sources4
Beta strandi639 – 641Combined sources3
Helixi654 – 661Combined sources8
Helixi667 – 669Combined sources3
Beta strandi670 – 672Combined sources3
Beta strandi674 – 682Combined sources9
Helixi683 – 689Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LKXX-ray3.00A/B/C/D1-697[»]
4A7Felectron microscopy7.70C/G/J1-697[»]
4A7Helectron microscopy7.80C/I/J1-697[»]
4A7Lelectron microscopy8.10C/G/J1-697[»]
ProteinModelPortaliQ03479
SMRiQ03479
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03479

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 693Myosin motorPROSITE-ProRule annotationAdd BLAST686
Domaini694 – 722IQ 1PROSITE-ProRule annotationAdd BLAST29
Domaini716 – 745IQ 2PROSITE-ProRule annotationAdd BLAST30
Domaini810 – 1004TH1PROSITE-ProRule annotationAdd BLAST195

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni556 – 630Actin-bindingAdd BLAST75

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0164 Eukaryota
COG5022 LUCA
InParanoidiQ03479
KOiK10356
OMAiAMWKAGH
PhylomeDBiQ03479

Family and domain databases

CDDicd01378 MYSc_Myo1, 1 hit
Gene3Di3.40.850.10, 3 hits
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR010926 Myosin_TH1
IPR036072 MYSc_Myo1
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00612 IQ, 2 hits
PF00063 Myosin_head, 1 hit
PF06017 Myosin_TH1, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00015 IQ, 1 hit
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 2 hits
PS51456 MYOSIN_MOTOR, 1 hit
PS51757 TH1, 1 hit

Sequencei

Sequence statusi: Complete.

Q03479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPKTKAEGV PDFVLLNQIT ENAFIENLTM RHKSDNIYTY IGDVVISTNP
60 70 80 90 100
FKNLNIYKES DIKAYNGRYK YEMPPHIYAL ANDAYRSMRQ SQENQCVIIS
110 120 130 140 150
GESGAGKTEA SKKIMQFLTF VSSNQSPNGE RISKMLLDSN PLLEAFGNAK
160 170 180 190 200
TLRNDNSSRF GKYMEMQFNA VGSPIGGKIT NYLLEKSRVV GRTQGERSFH
210 220 230 240 250
IFYQMLKGLS QSKLNELGLT PNAPAYEYLK KSGCFDVSTI DDSGEFKIIV
260 270 280 290 300
KAMETLGLKE SDQNSIWRIL AAILHIGNIT FAEAAEQRTG TTTVKVSDTK
310 320 330 340 350
SLAAAASCLK TDQQSLSIAL CYRSISTGVG KRCSVISVPM DCNQAAYSRD
360 370 380 390 400
ALAKALYERL FNWLVSKINT IINCTTEKGP VIGILDIYGF EVFQNNSFEQ
410 420 430 440 450
LNINFCNEKL QQLFIELTLK SEQEEYVREG IEWKNIEYFN NKPICELIEK
460 470 480 490 500
KPIGLISLLD EACLIAKSTD QTFLDSICKQ FEKNPHLQSY VVSKDRSIGD
510 520 530 540 550
TCFRLKHYAG DVTYDVRGFL DKNKDTLFGD LISSMQSSSD PLVQGLFPPT
560 570 580 590 600
RPEDSKKRPE TAGSQFRNAM NALITTLLAC SPHYVRCIKS NDNKQAGVID
610 620 630 640 650
EDRVRHQVRY LGLLENVRVR RAGFAGRIEY TRFYNRYKML CKKTWPSFNG
660 670 680 690 700
TAKQATELIL QQHNIDKEEI RMGKTKVFIR NPTTLFYFEE KRELEMPRIV
710 720 730 740 750
TLIQKTWRGY RARSKWNQRK AAIKIQLFYR SYRYKKWFRE LHRAFKDVAR
760 770 780 790 800
DPQWGKQVFW PKHPSILDRA VQLTHKIHNC WRAEKMILSL GAGQNHMRQK
810 820 830 840 850
VMAYDIFHGK KKWDFRRHFD ADYLEKPSNP NQQKYVLAMQ NLFSTYGDTE
860 870 880 890 900
VLFADYVIKV NPKGVPQRRG IVVTGTNIYK HDPKNYKVKK WGTPLVDVTS
910 920 930 940 950
ISISPMADTF LVLHCKAPQR DFVLDLGCNG YEAVSEITTV IVQQVLKLTG
960 970 980 990 1000
VKLSVQFTSS ITYNNARPKG SDTILTFAPI NNDPKLIGSQ FKKGKGNQAT

IQFKD
Length:1,005
Mass (Da):114,795
Last modified:December 4, 2007 - v2
Checksum:i8E3D638C7043CF0A
GO

Sequence cautioni

The sequence AAA33201 differs from that shown. Reason: Frameshift at positions 136, 370, 376 and 380.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26E → D in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti48T → R in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti427 – 429VRE → K in AAA33201 (PubMed:8504170).Curated3
Sequence conflicti440Missing in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti498I → N in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti604V → D in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti681 – 683NPT → IPR in AAA33201 (PubMed:8504170).Curated3
Sequence conflicti735K → N in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti763H → D in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti828S → W in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti889K → N in AAA33201 (PubMed:8504170).Curated1
Sequence conflicti997 – 998NQ → KE in AAA33201 (PubMed:8504170).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06805 Genomic DNA Translation: AAA33201.1 Frameshift.
AAFI02000120 Genomic DNA Translation: EAL63071.1
PIRiS33760
RefSeqiXP_636580.1, XM_631488.1

Genome annotation databases

EnsemblProtistsiEAL63071; EAL63071; DDB_G0288679
GeneIDi8626753
KEGGiddi:DDB_G0288679

Similar proteinsi

Entry informationi

Entry nameiMYOE_DICDI
AccessioniPrimary (citable) accession number: Q03479
Secondary accession number(s): Q54IK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health