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Protein

Myosin IE heavy chain

Gene

myoE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Myosin is a protein that binds to actin and has ATPase activity that is activated by actin. May play a role in moving membranes relative to actin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi101 – 1088ATP

GO - Molecular functioni

  • actin binding Source: dictyBase
  • actin-dependent ATPase activity Source: dictyBase
  • ATP binding Source: dictyBase
  • microfilament motor activity Source: dictyBase

GO - Biological processi

  • phagocytosis Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin IE heavy chain
Gene namesi
Name:myoE
Synonyms:dmiE
ORF Names:DDB_G0288679
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288679. myoE.

Subcellular locationi

GO - Cellular componenti

  • cell-cell contact zone Source: dictyBase
  • cell leading edge Source: dictyBase
  • early phagosome Source: dictyBase
  • macropinocytic cup Source: dictyBase
  • myosin I complex Source: dictyBase
  • phagocytic cup Source: dictyBase
  • plasma membrane Source: dictyBase
  • pseudopodium Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10051005Myosin IE heavy chainPRO_0000123369Add
BLAST

Proteomic databases

PaxDbiQ03479.
PRIDEiQ03479.

Interactioni

Subunit structurei

Myosin I heavy chain is single-headed. Dimer of a heavy and a light chain. Inability to self-assemble into filaments.

GO - Molecular functioni

  • actin binding Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0216200.

Structurei

Secondary structure

1
1005
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 153Combined sources
Helixi21 – 3313Combined sources
Beta strandi38 – 436Combined sources
Beta strandi45 – 484Combined sources
Helixi59 – 657Combined sources
Helixi70 – 723Combined sources
Helixi77 – 9115Combined sources
Beta strandi95 – 1006Combined sources
Helixi107 – 12115Combined sources
Helixi127 – 14721Combined sources
Beta strandi160 – 1689Combined sources
Beta strandi174 – 1829Combined sources
Helixi186 – 1894Combined sources
Helixi200 – 2067Combined sources
Helixi211 – 2177Combined sources
Helixi223 – 2253Combined sources
Helixi227 – 2304Combined sources
Turni231 – 2333Combined sources
Helixi242 – 25514Combined sources
Helixi260 – 27617Combined sources
Beta strandi281 – 2844Combined sources
Turni288 – 2903Combined sources
Beta strandi293 – 2986Combined sources
Helixi299 – 30911Combined sources
Helixi313 – 3219Combined sources
Helixi342 – 37231Combined sources
Beta strandi381 – 3866Combined sources
Beta strandi394 – 3963Combined sources
Helixi398 – 41821Combined sources
Helixi420 – 4267Combined sources
Helixi443 – 4475Combined sources
Beta strandi450 – 4545Combined sources
Helixi455 – 46410Combined sources
Helixi470 – 48011Combined sources
Beta strandi481 – 4844Combined sources
Turni490 – 4923Combined sources
Beta strandi502 – 5076Combined sources
Beta strandi510 – 5156Combined sources
Helixi519 – 5246Combined sources
Helixi529 – 5368Combined sources
Helixi541 – 5466Combined sources
Helixi562 – 57716Combined sources
Beta strandi580 – 5889Combined sources
Helixi601 – 61010Combined sources
Helixi614 – 6229Combined sources
Helixi631 – 6344Combined sources
Beta strandi639 – 6413Combined sources
Helixi654 – 6618Combined sources
Helixi667 – 6693Combined sources
Beta strandi670 – 6723Combined sources
Beta strandi674 – 6829Combined sources
Helixi683 – 6897Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LKXX-ray3.00A/B/C/D1-697[»]
4A7Felectron microscopy7.70C/G/J1-697[»]
4A7Helectron microscopy7.80C/I/J1-697[»]
4A7Lelectron microscopy8.10C/G/J1-697[»]
ProteinModelPortaliQ03479.
SMRiQ03479. Positions 9-697.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03479.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 693686Myosin motorAdd
BLAST
Domaini694 – 72229IQ 1PROSITE-ProRule annotationAdd
BLAST
Domaini716 – 74530IQ 2PROSITE-ProRule annotationAdd
BLAST
Domaini810 – 1004195TH1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni556 – 63075Actin-bindingAdd
BLAST

Sequence similaritiesi

Contains 2 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 1 TH1 (class I myosin tail homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0164. Eukaryota.
COG5022. LUCA.
InParanoidiQ03479.
KOiK10356.
OMAiLANDAYR.
PhylomeDBiQ03479.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00612. IQ. 2 hits.
PF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 2 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPKTKAEGV PDFVLLNQIT ENAFIENLTM RHKSDNIYTY IGDVVISTNP
60 70 80 90 100
FKNLNIYKES DIKAYNGRYK YEMPPHIYAL ANDAYRSMRQ SQENQCVIIS
110 120 130 140 150
GESGAGKTEA SKKIMQFLTF VSSNQSPNGE RISKMLLDSN PLLEAFGNAK
160 170 180 190 200
TLRNDNSSRF GKYMEMQFNA VGSPIGGKIT NYLLEKSRVV GRTQGERSFH
210 220 230 240 250
IFYQMLKGLS QSKLNELGLT PNAPAYEYLK KSGCFDVSTI DDSGEFKIIV
260 270 280 290 300
KAMETLGLKE SDQNSIWRIL AAILHIGNIT FAEAAEQRTG TTTVKVSDTK
310 320 330 340 350
SLAAAASCLK TDQQSLSIAL CYRSISTGVG KRCSVISVPM DCNQAAYSRD
360 370 380 390 400
ALAKALYERL FNWLVSKINT IINCTTEKGP VIGILDIYGF EVFQNNSFEQ
410 420 430 440 450
LNINFCNEKL QQLFIELTLK SEQEEYVREG IEWKNIEYFN NKPICELIEK
460 470 480 490 500
KPIGLISLLD EACLIAKSTD QTFLDSICKQ FEKNPHLQSY VVSKDRSIGD
510 520 530 540 550
TCFRLKHYAG DVTYDVRGFL DKNKDTLFGD LISSMQSSSD PLVQGLFPPT
560 570 580 590 600
RPEDSKKRPE TAGSQFRNAM NALITTLLAC SPHYVRCIKS NDNKQAGVID
610 620 630 640 650
EDRVRHQVRY LGLLENVRVR RAGFAGRIEY TRFYNRYKML CKKTWPSFNG
660 670 680 690 700
TAKQATELIL QQHNIDKEEI RMGKTKVFIR NPTTLFYFEE KRELEMPRIV
710 720 730 740 750
TLIQKTWRGY RARSKWNQRK AAIKIQLFYR SYRYKKWFRE LHRAFKDVAR
760 770 780 790 800
DPQWGKQVFW PKHPSILDRA VQLTHKIHNC WRAEKMILSL GAGQNHMRQK
810 820 830 840 850
VMAYDIFHGK KKWDFRRHFD ADYLEKPSNP NQQKYVLAMQ NLFSTYGDTE
860 870 880 890 900
VLFADYVIKV NPKGVPQRRG IVVTGTNIYK HDPKNYKVKK WGTPLVDVTS
910 920 930 940 950
ISISPMADTF LVLHCKAPQR DFVLDLGCNG YEAVSEITTV IVQQVLKLTG
960 970 980 990 1000
VKLSVQFTSS ITYNNARPKG SDTILTFAPI NNDPKLIGSQ FKKGKGNQAT

IQFKD
Length:1,005
Mass (Da):114,795
Last modified:December 4, 2007 - v2
Checksum:i8E3D638C7043CF0A
GO

Sequence cautioni

The sequence AAA33201.1 differs from that shown. Reason: Frameshift at positions 136, 370, 376 and 380. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261E → D in AAA33201 (PubMed:8504170).Curated
Sequence conflicti48 – 481T → R in AAA33201 (PubMed:8504170).Curated
Sequence conflicti427 – 4293VRE → K in AAA33201 (PubMed:8504170).Curated
Sequence conflicti440 – 4401Missing in AAA33201 (PubMed:8504170).Curated
Sequence conflicti498 – 4981I → N in AAA33201 (PubMed:8504170).Curated
Sequence conflicti604 – 6041V → D in AAA33201 (PubMed:8504170).Curated
Sequence conflicti681 – 6833NPT → IPR in AAA33201 (PubMed:8504170).Curated
Sequence conflicti735 – 7351K → N in AAA33201 (PubMed:8504170).Curated
Sequence conflicti763 – 7631H → D in AAA33201 (PubMed:8504170).Curated
Sequence conflicti828 – 8281S → W in AAA33201 (PubMed:8504170).Curated
Sequence conflicti889 – 8891K → N in AAA33201 (PubMed:8504170).Curated
Sequence conflicti997 – 9982NQ → KE in AAA33201 (PubMed:8504170).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06805 Genomic DNA. Translation: AAA33201.1. Frameshift.
AAFI02000120 Genomic DNA. Translation: EAL63071.1.
PIRiS33760.
RefSeqiXP_636580.1. XM_631488.1.

Genome annotation databases

EnsemblProtistsiDDB0216200; DDB0216200; DDB_G0288679.
GeneIDi8626753.
KEGGiddi:DDB_G0288679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06805 Genomic DNA. Translation: AAA33201.1. Frameshift.
AAFI02000120 Genomic DNA. Translation: EAL63071.1.
PIRiS33760.
RefSeqiXP_636580.1. XM_631488.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LKXX-ray3.00A/B/C/D1-697[»]
4A7Felectron microscopy7.70C/G/J1-697[»]
4A7Helectron microscopy7.80C/I/J1-697[»]
4A7Lelectron microscopy8.10C/G/J1-697[»]
ProteinModelPortaliQ03479.
SMRiQ03479. Positions 9-697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0216200.

Proteomic databases

PaxDbiQ03479.
PRIDEiQ03479.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0216200; DDB0216200; DDB_G0288679.
GeneIDi8626753.
KEGGiddi:DDB_G0288679.

Organism-specific databases

dictyBaseiDDB_G0288679. myoE.

Phylogenomic databases

eggNOGiKOG0164. Eukaryota.
COG5022. LUCA.
InParanoidiQ03479.
KOiK10356.
OMAiLANDAYR.
PhylomeDBiQ03479.

Miscellaneous databases

EvolutionaryTraceiQ03479.
PROiQ03479.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00612. IQ. 2 hits.
PF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 2 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Dictyostelium myosin IE heavy chain gene encodes a truncated isoform that lacks sequences corresponding to the actin binding site in the tail."
    Urrutia R., Jung G., Hammer J.A. III
    Biochim. Biophys. Acta 1173:225-229(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Thirteen is enough: the myosins of Dictyostelium discoideum and their light chains."
    Kollmar M.
    BMC Genomics 7:183-183(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  4. "Crystal structure of the motor domain of a class-I myosin."
    Kollmar M., Duerrwang U., Kliche W., Manstein D.J., Kull F.J.
    EMBO J. 21:2517-2525(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-695.

Entry informationi

Entry nameiMYOE_DICDI
AccessioniPrimary (citable) accession number: Q03479
Secondary accession number(s): Q54IK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 4, 2007
Last modified: April 13, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.