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Q03471

- ARIS_PENRO

UniProt

Q03471 - ARIS_PENRO

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Protein
Aristolochene synthase
Gene
ARI1
Organism
Penicillium roqueforti
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic sesquiterpene aristolochene. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce aristolochene. Aristolochene is the likely parent compound for a number of sesquiterpenoid toxins produced by filamentous fungi.2 Publications

Catalytic activityi

(2E,6E)-farnesyl diphosphate = aristolochene + diphosphate.1 Publication

Cofactori

Binds 3 magnesium ions per subunit By similarity.

Kineticsi

  1. KM=0.6 µM for (2E,6E)-farnesyl diphosphate1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151Magnesium 1 By similarity
Metal bindingi115 – 1151Magnesium 2 By similarity
Binding sitei200 – 2001Substrate By similarity
Metal bindingi244 – 2441Magnesium 3 By similarity
Metal bindingi248 – 2481Magnesium 3 By similarity
Binding sitei251 – 2511Substrate By similarity
Metal bindingi252 – 2521Magnesium 3 By similarity

GO - Molecular functioni

  1. aristolochene synthase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16547.
BRENDAi4.2.3.9. 8737.
UniPathwayiUPA00177; UER00582.

Names & Taxonomyi

Protein namesi
Recommended name:
Aristolochene synthase (EC:4.2.3.9)
Short name:
AS
Alternative name(s):
Sesquiterpene cyclase
Gene namesi
Name:ARI1
OrganismiPenicillium roqueforti
Taxonomic identifieri5082 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicillium

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi92 – 921Y → F: Causes 100-fold reduction in kcat but a 50-fold decrease in KM, resulting in a 2-fold decrease in catalytic efficiency. 1 Publication
Mutagenesisi115 – 1151D → N: Abolishes catalytic activity. 1 Publication
Mutagenesisi244 – 2441N → L: Abolishes catalytic activity. 1 Publication
Mutagenesisi248 – 2481S → A: Abolishes catalytic activity; when associated with D-252. 1 Publication
Mutagenesisi252 – 2521E → D: Abolishes catalytic activity; when associated with A-248. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Aristolochene synthase
PRO_0000064677Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi54 – 6815
Helixi74 – 8310
Helixi85 – 928
Turni98 – 1003
Helixi101 – 12020
Helixi123 – 13715
Helixi148 – 18033
Turni181 – 1833
Helixi193 – 2019
Helixi204 – 21714
Helixi223 – 2275
Helixi230 – 24819
Turni249 – 2524
Helixi269 – 2779
Helixi281 – 30727
Helixi315 – 33622

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DGPX-ray2.80A/B40-339[»]
1DI1X-ray2.50A/B40-339[»]
1F1Kmodel-A1-342[»]
1F1Lmodel-A1-342[»]
1F1Nmodel-A1-342[»]
1F1Pmodel-A1-342[»]
ProteinModelPortaliQ03471.
SMRiQ03471. Positions 40-339.

Miscellaneous databases

EvolutionaryTraceiQ03471.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni340 – 3412Substrate-binding By similarity

Sequence similaritiesi

Belongs to the terpene synthase family.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03471-1 [UniParc]FASTAAdd to Basket

« Hide

MATSTETISS LAQPFVHLEN PINSPLVKET IRPRNDTTIT PPPTQWSYLC    50
HPRVKEVQDE VDGYFLENWK FPSFKAVRTF LDAKFSEVTC LYFPLALDDR 100
IHFACRLLTV LFLIDDVLEH MSFADGEAYN NRLIPISRGD VLPDRTKPEE 150
FILYDLWESM RAHDAELANE VLEPTFVFMR AQTDRARLSI HELGHYLEYR 200
EKDVGKALLS ALMRFSMGLR LSADELQDMK ALEANCAKQL SVVNDIYSYD 250
KEEEASRTGH KEGAFLCSAV KVLAEESKLG IPATKRVLWS MTREWETVHD 300
EIVAEKIASP DGCSEAAKAY MKGLEYQMSG NEQWSKTTRR YN 342
Length:342
Mass (Da):39,192
Last modified:October 1, 1993 - v1
Checksum:iACFBD63F4FF9BB03
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05193 Genomic DNA. Translation: AAA33694.1.
PIRiA45462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05193 Genomic DNA. Translation: AAA33694.1 .
PIRi A45462.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DGP X-ray 2.80 A/B 40-339 [» ]
1DI1 X-ray 2.50 A/B 40-339 [» ]
1F1K model - A 1-342 [» ]
1F1L model - A 1-342 [» ]
1F1N model - A 1-342 [» ]
1F1P model - A 1-342 [» ]
ProteinModelPortali Q03471.
SMRi Q03471. Positions 40-339.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00177 ; UER00582 .
BioCyci MetaCyc:MONOMER-16547.
BRENDAi 4.2.3.9. 8737.

Miscellaneous databases

EvolutionaryTracei Q03471.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Aristolochene synthase. Isolation, characterization, and bacterial expression of a sesquiterpenoid biosynthetic gene (Ari1) from Penicillium roqueforti."
    Proctor R.H., Hohn T.M.
    J. Biol. Chem. 268:4543-4548(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 56-70; 76-84; 245-251 AND 323-336, FUNCTION, CATALYTIC ACTIVITY.
    Strain: ATCC 10110 / CBS 221.30 / NBRC 5459 / NRRL 849.
  2. "Aristolochene synthase: mechanistic analysis of active site residues by site-directed mutagenesis."
    Felicetti B., Cane D.E.
    J. Am. Chem. Soc. 126:7212-7221(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF TYR-92; ASP-115; ASN-244; SER-248 AND GLU-252.
  3. "Crystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti."
    Caruthers J.M., Kang I., Rynkiewicz M.J., Cane D.E., Christianson D.W.
    J. Biol. Chem. 275:25533-25539(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiARIS_PENRO
AccessioniPrimary (citable) accession number: Q03471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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