Reviewed, UniProtKB/Swiss-Prot Q03470 (GYRA_CAMJE)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	Cj1027c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	863 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 863	863	DNA gyrase subunit A		PRO_0000145227
Sites
	Active site	125	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Natural variations
	Natural variant	70	1	A → T in strain: UA67; confers resistance to nalidixic acid and ciprofloxacin.
	Natural variant	86	1	T → I in strain: UA580R1, UA536, UA543 and UA549; confers resistance to nalidixic acid and ciprofloxacin.
	Natural variant	90	1	D → N in strain: UA580R3; confers resistance to nalidixic acid and ciprofloxacin.

Sequences

	Sequence		Length	Mass (Da)
	Q03470-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: CB8C577F8D6EAF8B	FASTA	863	96,974
	10 20 30 40 50 60 MENIFSKDSD IELVDIENSI KSSYLDYSMS VIIGRALPDA RDGLKPVHRR ILYAMQNDEA 70 80 90 100 110 120 KSRTDFVKSA RIVGAVIGRY HPHGDTAVYD ALVRMAQDFS MRYPSITGQG NFGSIDGDSA 130 140 150 160 170 180 AAMRYTEAKM SKLSHELLKD IDKDTVDFVP NYDGSESEPD VLPSRVPNLL LNGSSGIAVG 190 200 210 220 230 240 MATNIPPHSL NELIDGLLYL LDNKDASLEE IMQFIKGPDF PTGGIIYGKK GIIEAYRTGR 250 260 270 280 290 300 GRVKVRAKTH IEKKTNKDVI VIDELPYQTN KARLIEQIAE LVKERQIEGI SEVRDESNKE 310 320 330 340 350 360 GIRVVIELKR EAMSEIVLNN LFKSTTMEST FGVIMLAIHN KEPKIFSLLE LLNLFLTHRK 370 380 390 400 410 420 TVIIRRTIFE LQKARARAHI LEGLKIALDN IDEVIALIKN SSDNNTARDS LVAKFGLSEL 430 440 450 460 470 480 QANAILDMKL GRLTGLEREK IENELAELMK EIARLEEILK SETLLENLIR DELKEIRSKF 490 500 510 520 530 540 DVPRITQIED DYDDIDIEDL IPNENMVVTI THRGYIKRVP SKQYEKQKRG GKGKLAVTTY 550 560 570 580 590 600 DDDFIESFFT ANTHDTLMFV TDRGQLYWLK VYKIPEGSRT AKGKAVVNLI NLQAEEKIMA 610 620 630 640 650 660 IIPTTDFDES KSLCFFTKNG IVKRTNLSEY QNIRSVGVRA INLDENDELV TAIIVQRDED 670 680 690 700 710 720 EIFATGGEEN LENQEIENLD DENLENEESV STQGKMLFAV TKKGMCIKFP LAKVREIGRV 730 740 750 760 770 780 SRGVTAIKFK EKNDELVGAV VIENDEQEIL SISAKGIGKR TNAGEYRLQS RGGKGVICMK 790 800 810 820 830 840 LTEKTKDLIS VVIVDETMDL MALTSSGKMI RVDMQSIRKA GRNTSGVIVV NVENDEVVSI 850 860 AKCPKEENDE DELSDENFGL DLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the Campylobacter jejuni gyrA gene and characterization of quinolone resistance mutations." Wang Y., Huang W.M., Taylor D.E. Antimicrob. Agents Chemother. 37:457-463(1993) [PubMed: 8384814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: UA580.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L04566 Genomic DNA. Translation: AAA23028.1. AL111168 Genomic DNA. Translation: CAL35145.1.
PIR	A48902.
RefSeq	YP_002344422.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03470. 23 interactions.
Genome annotation databases
GeneID	905319.
GenomeReviews	Gene locus Cj1027c in contig AL111168_GR.
KEGG	cje:Cj1027c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1027C-MON.
BRENDA	5.99.1.3. 255835.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_CAMJE

Accession

Primary (citable) accession number: Q03470
Secondary accession number(s): Q0P9M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)