Q03467 (E13B_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glucan endo-1,3-beta-glucosidase EC=3.2.1.39 Alternative name(s): (1->3)-beta-glucan endohydrolase Short name=(1->3)-beta-glucanase Beta-1,3-endoglucanase
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Tissue specificity	Constitutively expressed in seedling roots.
Induction	By fungal elicitors.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 347	315	Glucan endo-1,3-beta-glucosidase		PRO_0000011855
	Propeptide	348 – 370	23	Removed in mature form By similarity		PRO_0000011856
Sites
	Active site	272	1	Nucleophile By similarity
	Active site	328	1	Proton donor By similarity
Amino acid modifications
	Modified residue	33	1	Pyrrolidone carboxylic acid Probable
	Glycosylation	361	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03467 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 93847A4CF99B6186	FASTA	370	41,050
	10 20 30 40 50 60 MASFFARTRR FSLVSLFLLE LFTINLIPTT DAQIGICYGM MGNNLPPANE VIALYKANNI 70 80 90 100 110 120 KRMRLYDPNQ PALNALRDSG IELILGIPNS DLQTLATNQD SARQWVQRNV LNFYPSVKIK 130 140 150 160 170 180 YIAVGNEVSP VGGSSWLAQY VLPATQNVYQ AIRAQGLHDQ IKVTTAIDMT LIGNSFPPSK 190 200 210 220 230 240 GSFRSDVRSY LDPFIGYLVY AGAPLLVNVY PYFSHIGNPR DISLPYALFT SPGVMVQDGP 250 260 270 280 290 300 NGYQNLFDAM LDSVHAALDN TGIGWVNVVV SESGWPSDGG SATSYDNARI YLDNLIRHVG 310 320 330 340 350 360 KGTPRRPWAT EAYLFAMFDE NQKSPELEKH FGVFYPNKQK KYPFGFGGER RDGEIVEGDF 370 NGTVSLKSDM

References

[1]	"Nucleotide sequence of a pea (Pisum sativum L.) beta-1,3-glucanase gene." Chang M.-M., Culley D.E., Hadwiger L.A. Plant Physiol. 101:1121-1122(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Alcan. Tissue: Leaf.
[2]	"Molecular characterization of a pea beta-1,3-glucanase induced by Fusarium solani and chitosan challenge." Chang M.-M., Hadwiger L.A., Horovitz D. Plant Mol. Biol. 20:609-618(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 32-370. Strain: cv. Alcan. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S51479 Genomic DNA. Translation: AAB24398.1. L02212 Genomic DNA. Translation: AAA33648.1. S69419 Genomic DNA. No translation available.
PIR	T06552.
3D structure databases
ProteinModelPortal	Q03467.
SMR	Q03467. Positions 34-346.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_PEA

Accession

Primary (citable) accession number: Q03467

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program