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Q03465

- RPN4_YEAST

UniProt

Q03465 - RPN4_YEAST

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Protein
Protein RPN4
Gene
RPN4, SON1, UFD5, YDL020C, D2840
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator of a number of genes encoding proteasomal subunits. Binds to a PACE (proteasome-associated control element) DNA sequence 5'-GGTGGCAAA-3'. Its expression is in turn regulated by the 26S proteasome, thereby providing a negative feedback control mechanism. Required for normal growth at low temperatures.2 Publications

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
  3. metal ion binding Source: InterPro
  4. protein binding Source: IntAct

GO - Biological processi

  1. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  2. positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  4. positive regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
  5. regulation of DNA repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29449-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RPN4
Alternative name(s):
Nuclear protein SON1
Ubiquitin fusion degradation protein 5
Gene namesi
Name:RPN4
Synonyms:SON1, UFD5
Ordered Locus Names:YDL020C
ORF Names:D2840
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL020c.
SGDiS000002178. RPN4.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutants grow slowly at low temperatures and show partial mislocalization of nuclear antigens.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Protein RPN4
PRO_0000173871Add
BLAST

Post-translational modificationi

Ubiquitinated by UBR2 in the presence of UBC2; which leads to proteasomal degradation.2 Publications

Keywords - PTMi

Ubl conjugation

Expressioni

Gene expression databases

GenevestigatoriQ03465.

Interactioni

Subunit structurei

Probably interacts with SEC63. Interacts with MUB1, UBR2 and RPN2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MUB1Q031623EBI-15931,EBI-28207
UBR2Q079632EBI-15931,EBI-34338

Protein-protein interaction databases

BioGridi32035. 514 interactions.
DIPiDIP-5779N.
IntActiQ03465. 5 interactions.
MINTiMINT-583572.
STRINGi4932.YDL020C.

Structurei

3D structure databases

ProteinModelPortaliQ03465.
SMRiQ03465. Positions 426-508.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi382 – 39817Nuclear localization signal Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi211 – 22919Asp/Glu-rich (acidic)
Add
BLAST
Compositional biasi300 – 31516Asp/Glu-rich (acidic)
Add
BLAST

Phylogenomic databases

eggNOGiNOG70197.
HOGENOMiHOG000144400.
KOiK03034.
OMAiNKYADPT.
OrthoDBiEOG7TF7PD.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03465-1 [UniParc]FASTAAdd to Basket

« Hide

MASTELSLKR TLTDILEDEL YHTNPGHSQF TSHYQNYHPN ASITPYKLVN    50
KNKENNTFTW NHSLQHQNES SAASIPPQQT YHFPIFNKYA DPTLTTTTSF 100
TTSEATANDR QINNVHLIPN EIKGASETPL QKTVNLKNIM KVSDPYVPTR 150
NTFNYDVKIS NDFFDNGDNL YGNDEEVLFY EDNYNPKMQW SLQDNSAAIN 200
NEDARAIFNN EFDSDDDDIS DDEEDEIEEN CLQQEQHQEE PLLSLDVTPI 250
SMFGSDQKTG RAKSSSHLFN EYSYVDSNMD SISSVVSEDL LDERGHEKIE 300
DEDEDNDLDE DDIYDISLLK NRRKQSFVLN KNTIDFERFP SPSTSANVPS 350
TATTGKRKPA KSSSNRSCVS NSNENGTLER IKKPTSAVVS SNASRRKLIN 400
YTKKHLSSHS STNSNSKPST ASPSAHTSSS DGNNEIFTCQ IMNLITNEPC 450
GAQFSRSYDL TRHQNTIHAK RKIVFRCSEC IKILGSEGYQ KTFSRLDALT 500
RHIKSKHEDL SLEQRQEVTK FAKANIGYVM G 531
Length:531
Mass (Da):60,153
Last modified:October 1, 1993 - v1
Checksum:i4316281AC09FBE7F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 2311C → Y in AAU09680. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00928 Genomic DNA. Translation: AAA35067.1.
Z48432 Genomic DNA. Translation: CAA88339.1.
Z74068 Genomic DNA. Translation: CAA98579.1.
AY723763 Genomic DNA. Translation: AAU09680.1.
BK006938 Genomic DNA. Translation: DAA11830.1.
PIRiS41986.
RefSeqiNP_010264.3. NM_001180079.3.

Genome annotation databases

EnsemblFungiiYDL020C; YDL020C; YDL020C.
GeneIDi851542.
KEGGisce:YDL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00928 Genomic DNA. Translation: AAA35067.1 .
Z48432 Genomic DNA. Translation: CAA88339.1 .
Z74068 Genomic DNA. Translation: CAA98579.1 .
AY723763 Genomic DNA. Translation: AAU09680.1 .
BK006938 Genomic DNA. Translation: DAA11830.1 .
PIRi S41986.
RefSeqi NP_010264.3. NM_001180079.3.

3D structure databases

ProteinModelPortali Q03465.
SMRi Q03465. Positions 426-508.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32035. 514 interactions.
DIPi DIP-5779N.
IntActi Q03465. 5 interactions.
MINTi MINT-583572.
STRINGi 4932.YDL020C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL020C ; YDL020C ; YDL020C .
GeneIDi 851542.
KEGGi sce:YDL020C.

Organism-specific databases

CYGDi YDL020c.
SGDi S000002178. RPN4.

Phylogenomic databases

eggNOGi NOG70197.
HOGENOMi HOG000144400.
KOi K03034.
OMAi NKYADPT.
OrthoDBi EOG7TF7PD.

Enzyme and pathway databases

BioCyci YEAST:G3O-29449-MONOMER.

Miscellaneous databases

NextBioi 968949.

Gene expression databases

Genevestigatori Q03465.

Family and domain databases

InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 2 hits.
[Graphical view ]
PROSITEi PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Extragenic suppressors of mutations in the cytoplasmic C-terminus of SEC63 define five genes in Saccharomyces cerevisiae."
    Nelson M.K., Kurihara T., Silver P.A.
    Genetics 134:159-173(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    Strain: ATCC 200060 / W303.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "A proteolytic pathway that recognizes ubiquitin as a degradation signal."
    Johnson E.S., Ma P.C.M., Ota I.M., Varshavsky A.
    J. Biol. Chem. 270:17442-17456(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 204508 / S288c.
  6. "Son1p is a component of the 26S proteasome of the yeast Saccharomyces cerevisiae."
    Fujimuro M., Tanaka K., Yokosawa H., Toh-e A.
    FEBS Lett. 423:149-154(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY CHARACTERIZATION.
  7. "Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast."
    Mannhaupt G., Schnall R., Karpov V., Vetter I., Feldmann H.
    FEBS Lett. 450:27-34(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "RPN4 is a ligand, substrate, and transcriptional regulator of the 26S proteasome: a negative feedback circuit."
    Xie Y., Varshavsky A.
    Proc. Natl. Acad. Sci. U.S.A. 98:3056-3061(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase."
    Wang L., Mao X., Ju D., Xie Y.
    J. Biol. Chem. 279:55218-55223(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, INTERACTION WITH UBR2.
  10. "Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation."
    Ju D., Wang X., Xu H., Xie Y.
    Mol. Cell. Biol. 28:1404-1412(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, INTERACTION WITH MUB1.

Entry informationi

Entry nameiRPN4_YEAST
AccessioniPrimary (citable) accession number: Q03465
Secondary accession number(s): D6VRX0, E9P944
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

According to 1 Publication, it is a component of the 26S proteasome.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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