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Protein

Protein RPN4

Gene

RPN4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator of a number of genes encoding proteasomal subunits. Binds to a PACE (proteasome-associated control element) DNA sequence 5'-GGTGGCAAA-3'. Its expression is in turn regulated by the 26S proteasome, thereby providing a negative feedback control mechanism. Required for normal growth at low temperatures.2 Publications

Caution

According to PubMed:9512348, it is a component of the 26S proteasome.Curated

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
  • regulation of DNA repair Source: SGD

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29449-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RPN4
Alternative name(s):
Nuclear protein SON1
Ubiquitin fusion degradation protein 5
Gene namesi
Name:RPN4
Synonyms:SON1, UFD5
Ordered Locus Names:YDL020C
ORF Names:D2840
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL020C
SGDiS000002178 RPN4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutants grow slowly at low temperatures and show partial mislocalization of nuclear antigens.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738711 – 531Protein RPN4Add BLAST531

Post-translational modificationi

Ubiquitinated by UBR2 in the presence of UBC2; which leads to proteasomal degradation.2 Publications

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ03465
PRIDEiQ03465

PTM databases

iPTMnetiQ03465

Interactioni

Subunit structurei

Probably interacts with SEC63. Interacts with MUB1, UBR2 and RPN2.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32035, 779 interactors
DIPiDIP-5779N
IntActiQ03465, 5 interactors
MINTiQ03465
STRINGi4932.YDL020C

Structurei

3D structure databases

ProteinModelPortaliQ03465
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi382 – 398Nuclear localization signalSequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi211 – 229Asp/Glu-rich (acidic)Add BLAST19
Compositional biasi300 – 315Asp/Glu-rich (acidic)Add BLAST16

Phylogenomic databases

HOGENOMiHOG000144400
InParanoidiQ03465
KOiK03034
OMAiSNTHTHI
OrthoDBiEOG092C2YCI

Family and domain databases

InterProiView protein in InterPro
IPR013087 Znf_C2H2_type
PROSITEiView protein in PROSITE
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q03465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTELSLKR TLTDILEDEL YHTNPGHSQF TSHYQNYHPN ASITPYKLVN
60 70 80 90 100
KNKENNTFTW NHSLQHQNES SAASIPPQQT YHFPIFNKYA DPTLTTTTSF
110 120 130 140 150
TTSEATANDR QINNVHLIPN EIKGASETPL QKTVNLKNIM KVSDPYVPTR
160 170 180 190 200
NTFNYDVKIS NDFFDNGDNL YGNDEEVLFY EDNYNPKMQW SLQDNSAAIN
210 220 230 240 250
NEDARAIFNN EFDSDDDDIS DDEEDEIEEN CLQQEQHQEE PLLSLDVTPI
260 270 280 290 300
SMFGSDQKTG RAKSSSHLFN EYSYVDSNMD SISSVVSEDL LDERGHEKIE
310 320 330 340 350
DEDEDNDLDE DDIYDISLLK NRRKQSFVLN KNTIDFERFP SPSTSANVPS
360 370 380 390 400
TATTGKRKPA KSSSNRSCVS NSNENGTLER IKKPTSAVVS SNASRRKLIN
410 420 430 440 450
YTKKHLSSHS STNSNSKPST ASPSAHTSSS DGNNEIFTCQ IMNLITNEPC
460 470 480 490 500
GAQFSRSYDL TRHQNTIHAK RKIVFRCSEC IKILGSEGYQ KTFSRLDALT
510 520 530
RHIKSKHEDL SLEQRQEVTK FAKANIGYVM G
Length:531
Mass (Da):60,153
Last modified:October 1, 1993 - v1
Checksum:i4316281AC09FBE7F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231C → Y in AAU09680 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00928 Genomic DNA Translation: AAA35067.1
Z48432 Genomic DNA Translation: CAA88339.1
Z74068 Genomic DNA Translation: CAA98579.1
AY723763 Genomic DNA Translation: AAU09680.1
BK006938 Genomic DNA Translation: DAA11830.1
PIRiS41986
RefSeqiNP_010264.3, NM_001180079.3

Genome annotation databases

EnsemblFungiiYDL020C; YDL020C; YDL020C
GeneIDi851542
KEGGisce:YDL020C

Similar proteinsi

Entry informationi

Entry nameiRPN4_YEAST
AccessioniPrimary (citable) accession number: Q03465
Secondary accession number(s): D6VRX0, E9P944
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health