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Protein

Protein RPN4

Gene

RPN4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator of a number of genes encoding proteasomal subunits. Binds to a PACE (proteasome-associated control element) DNA sequence 5'-GGTGGCAAA-3'. Its expression is in turn regulated by the 26S proteasome, thereby providing a negative feedback control mechanism. Required for normal growth at low temperatures.2 Publications

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
  • regulation of DNA repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29449-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RPN4
Alternative name(s):
Nuclear protein SON1
Ubiquitin fusion degradation protein 5
Gene namesi
Name:RPN4
Synonyms:SON1, UFD5
Ordered Locus Names:YDL020C
ORF Names:D2840
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL020C.
SGDiS000002178. RPN4.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutants grow slowly at low temperatures and show partial mislocalization of nuclear antigens.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738711 – 531Protein RPN4Add BLAST531

Post-translational modificationi

Ubiquitinated by UBR2 in the presence of UBC2; which leads to proteasomal degradation.2 Publications

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ03465.

PTM databases

iPTMnetiQ03465.

Interactioni

Subunit structurei

Probably interacts with SEC63. Interacts with MUB1, UBR2 and RPN2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MUB1Q031623EBI-15931,EBI-28207
UBR2Q079632EBI-15931,EBI-34338

Protein-protein interaction databases

BioGridi32035. 523 interactors.
DIPiDIP-5779N.
IntActiQ03465. 5 interactors.
MINTiMINT-583572.

Structurei

3D structure databases

ProteinModelPortaliQ03465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi382 – 398Nuclear localization signalSequence analysisAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi211 – 229Asp/Glu-rich (acidic)Add BLAST19
Compositional biasi300 – 315Asp/Glu-rich (acidic)Add BLAST16

Phylogenomic databases

HOGENOMiHOG000144400.
InParanoidiQ03465.
KOiK03034.
OMAiREYEENA.
OrthoDBiEOG092C2YCI.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PROSITEiPS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTELSLKR TLTDILEDEL YHTNPGHSQF TSHYQNYHPN ASITPYKLVN
60 70 80 90 100
KNKENNTFTW NHSLQHQNES SAASIPPQQT YHFPIFNKYA DPTLTTTTSF
110 120 130 140 150
TTSEATANDR QINNVHLIPN EIKGASETPL QKTVNLKNIM KVSDPYVPTR
160 170 180 190 200
NTFNYDVKIS NDFFDNGDNL YGNDEEVLFY EDNYNPKMQW SLQDNSAAIN
210 220 230 240 250
NEDARAIFNN EFDSDDDDIS DDEEDEIEEN CLQQEQHQEE PLLSLDVTPI
260 270 280 290 300
SMFGSDQKTG RAKSSSHLFN EYSYVDSNMD SISSVVSEDL LDERGHEKIE
310 320 330 340 350
DEDEDNDLDE DDIYDISLLK NRRKQSFVLN KNTIDFERFP SPSTSANVPS
360 370 380 390 400
TATTGKRKPA KSSSNRSCVS NSNENGTLER IKKPTSAVVS SNASRRKLIN
410 420 430 440 450
YTKKHLSSHS STNSNSKPST ASPSAHTSSS DGNNEIFTCQ IMNLITNEPC
460 470 480 490 500
GAQFSRSYDL TRHQNTIHAK RKIVFRCSEC IKILGSEGYQ KTFSRLDALT
510 520 530
RHIKSKHEDL SLEQRQEVTK FAKANIGYVM G
Length:531
Mass (Da):60,153
Last modified:October 1, 1993 - v1
Checksum:i4316281AC09FBE7F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231C → Y in AAU09680 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00928 Genomic DNA. Translation: AAA35067.1.
Z48432 Genomic DNA. Translation: CAA88339.1.
Z74068 Genomic DNA. Translation: CAA98579.1.
AY723763 Genomic DNA. Translation: AAU09680.1.
BK006938 Genomic DNA. Translation: DAA11830.1.
PIRiS41986.
RefSeqiNP_010264.3. NM_001180079.3.

Genome annotation databases

EnsemblFungiiYDL020C; YDL020C; YDL020C.
GeneIDi851542.
KEGGisce:YDL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00928 Genomic DNA. Translation: AAA35067.1.
Z48432 Genomic DNA. Translation: CAA88339.1.
Z74068 Genomic DNA. Translation: CAA98579.1.
AY723763 Genomic DNA. Translation: AAU09680.1.
BK006938 Genomic DNA. Translation: DAA11830.1.
PIRiS41986.
RefSeqiNP_010264.3. NM_001180079.3.

3D structure databases

ProteinModelPortaliQ03465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32035. 523 interactors.
DIPiDIP-5779N.
IntActiQ03465. 5 interactors.
MINTiMINT-583572.

PTM databases

iPTMnetiQ03465.

Proteomic databases

PRIDEiQ03465.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL020C; YDL020C; YDL020C.
GeneIDi851542.
KEGGisce:YDL020C.

Organism-specific databases

EuPathDBiFungiDB:YDL020C.
SGDiS000002178. RPN4.

Phylogenomic databases

HOGENOMiHOG000144400.
InParanoidiQ03465.
KOiK03034.
OMAiREYEENA.
OrthoDBiEOG092C2YCI.

Enzyme and pathway databases

BioCyciYEAST:G3O-29449-MONOMER.

Miscellaneous databases

PROiQ03465.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PROSITEiPS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPN4_YEAST
AccessioniPrimary (citable) accession number: Q03465
Secondary accession number(s): D6VRX0, E9P944
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

According to PubMed:9512348, it is a component of the 26S proteasome.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.