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Protein

Antiviral protein alpha

Gene
N/A
Organism
Phytolacca americana (American pokeweed) (Phytolacca decandra)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits viral infection of plants, and protein synthesis in vitro. Has also been shown to inhibit the replication of mammalian viruses. The protein may provide a means of cellular suicide upon invasion by a virus.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei199By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein synthesis inhibitor, Toxin

Keywords - Biological processi

Antiviral defense, Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Antiviral protein alpha (EC:3.2.2.22)
Alternative name(s):
PAP-alpha
Ribosome-inactivating protein
rRNA N-glycosidase
OrganismiPhytolacca americana (American pokeweed) (Phytolacca decandra)
Taxonomic identifieri3527 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesPhytolaccaceaePhytolacca

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24By similarityAdd BLAST24
ChainiPRO_000003077925 – 285Antiviral protein alphaAdd BLAST261
PropeptideiPRO_0000030780286 – 2949

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi58 ↔ 282
Disulfide bondi108 ↔ 130

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 31Combined sources4
Helixi32 – 34Combined sources3
Helixi37 – 51Combined sources15
Beta strandi61 – 63Combined sources3
Beta strandi69 – 71Combined sources3
Beta strandi73 – 79Combined sources7
Beta strandi85 – 91Combined sources7
Turni92 – 94Combined sources3
Beta strandi97 – 104Combined sources8
Beta strandi107 – 113Combined sources7
Helixi119 – 129Combined sources11
Beta strandi132 – 138Combined sources7
Helixi147 – 154Combined sources8
Helixi158 – 160Combined sources3
Helixi165 – 175Combined sources11
Helixi183 – 195Combined sources13
Helixi198 – 202Combined sources5
Helixi204 – 212Combined sources9
Turni213 – 215Combined sources3
Helixi222 – 240Combined sources19
Beta strandi245 – 253Combined sources9
Beta strandi259 – 264Combined sources6
Helixi265 – 268Combined sources4
Helixi269 – 271Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1APAX-ray2.30A22-285[»]
ProteinModelPortaliQ03464.
SMRiQ03464.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03464.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMMVVVVVM MLSWLILKPP STWAINTITF DVGNATINKY ATFMKSIHNQ
60 70 80 90 100
AKDPTLKCYG IPMLPNTNLT PKYLLVTLQD SSLKTITLML KRNNLYVMGY
110 120 130 140 150
ADTYNGKCRY HIFKDISNTT ERNDVMTTLC PNPSSRVGKN INYDSSYPAL
160 170 180 190 200
EKKVGRPRSQ VQLGIQILNS GIGKIYGVDS FTEKTEAEFL LVAIQMVSEA
210 220 230 240 250
ARFKYIENQV KTNFNRAFYP NAKVLNLEES WGKISTAIHN AKNGALTSPL
260 270 280 290
ELKNANGSKW IVLRVDDIEP DVGLLKYVNG TCQATYQSAM FPHL
Length:294
Mass (Da):33,069
Last modified:October 1, 1993 - v1
Checksum:iF2EC27724FA85596
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10600 Genomic DNA. Translation: BAA01451.1.
PIRiS28421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10600 Genomic DNA. Translation: BAA01451.1.
PIRiS28421.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1APAX-ray2.30A22-285[»]
ProteinModelPortaliQ03464.
SMRiQ03464.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ03464.

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIPA_PHYAM
AccessioniPrimary (citable) accession number: Q03464
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.