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Q03435

- NHP10_YEAST

UniProt

Q03435 - NHP10_YEAST

Protein

Non-histone protein 10

Gene

NHP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin remodeling complex.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi94 – 15865HMG boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA end binding Source: SGD
    2. heteroduplex DNA loop binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. double-strand break repair Source: SGD
    2. nucleosome mobilization Source: SGD
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. telomere maintenance via recombination Source: SGD
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29433-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-histone protein 10
    Alternative name(s):
    High mobility group protein 2
    Gene namesi
    Name:NHP10
    Synonyms:HMO2
    Ordered Locus Names:YDL002C
    ORF Names:YD8119.05C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL002c.
    SGDiS000002160. NHP10.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. Ino80 complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 203202Non-histone protein 10PRO_0000048567Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ03435.
    PaxDbiQ03435.
    PeptideAtlasiQ03435.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03435.

    Interactioni

    Subunit structurei

    Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IES1P435795EBI-12010,EBI-22775
    IES3Q123453EBI-12010,EBI-35758
    IES5P400605EBI-12010,EBI-22617
    INO80P5311511EBI-12010,EBI-24019

    Protein-protein interaction databases

    BioGridi32052. 321 interactions.
    DIPiDIP-1387N.
    IntActiQ03435. 28 interactions.
    MINTiMINT-388714.
    STRINGi4932.YDL002C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03435.
    SMRiQ03435. Positions 76-160.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5648.
    HOGENOMiHOG000001026.
    KOiK11680.
    OMAiAYLLFCE.
    OrthoDBiEOG70KH25.

    Family and domain databases

    Gene3Di1.10.30.10. 1 hit.
    InterProiIPR009071. HMG_box_dom.
    [Graphical view]
    PfamiPF00505. HMG_box. 1 hit.
    [Graphical view]
    SMARTiSM00398. HMG. 1 hit.
    [Graphical view]
    SUPFAMiSSF47095. SSF47095. 1 hit.
    PROSITEiPS50118. HMG_BOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03435-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVEEKKRRL EELKDQNVVL GLAIQRSRLS VKRLKLEYGV LLERLESRIE    50
    LDPELNCEDP LPTLASFKQE LLTKPFRKSK TKRHKVKERD PNMPKRPTNA 100
    YLLYCEMNKE RIRQNGSLDV TRDLAEGWKN LNEQDRKPYY KLYSEDRERY 150
    QMEMEIYNKK ISNIDADDDK EENEQKIKNN EEGSSTKVAD SKGGEDGSLV 200
    SSN 203
    Length:203
    Mass (Da):23,857
    Last modified:November 1, 1996 - v1
    Checksum:iEE6F488F78D32415
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48008 Genomic DNA. Translation: CAA88059.1.
    BK006938 Genomic DNA. Translation: DAA11846.1.
    PIRiS50980.
    RefSeqiNP_010282.3. NM_001180061.3.

    Genome annotation databases

    EnsemblFungiiYDL002C; YDL002C; YDL002C.
    GeneIDi851562.
    KEGGisce:YDL002C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48008 Genomic DNA. Translation: CAA88059.1 .
    BK006938 Genomic DNA. Translation: DAA11846.1 .
    PIRi S50980.
    RefSeqi NP_010282.3. NM_001180061.3.

    3D structure databases

    ProteinModelPortali Q03435.
    SMRi Q03435. Positions 76-160.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32052. 321 interactions.
    DIPi DIP-1387N.
    IntActi Q03435. 28 interactions.
    MINTi MINT-388714.
    STRINGi 4932.YDL002C.

    Proteomic databases

    MaxQBi Q03435.
    PaxDbi Q03435.
    PeptideAtlasi Q03435.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL002C ; YDL002C ; YDL002C .
    GeneIDi 851562.
    KEGGi sce:YDL002C.

    Organism-specific databases

    CYGDi YDL002c.
    SGDi S000002160. NHP10.

    Phylogenomic databases

    eggNOGi COG5648.
    HOGENOMi HOG000001026.
    KOi K11680.
    OMAi AYLLFCE.
    OrthoDBi EOG70KH25.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29433-MONOMER.

    Miscellaneous databases

    NextBioi 968998.

    Gene expression databases

    Genevestigatori Q03435.

    Family and domain databases

    Gene3Di 1.10.30.10. 1 hit.
    InterProi IPR009071. HMG_box_dom.
    [Graphical view ]
    Pfami PF00505. HMG_box. 1 hit.
    [Graphical view ]
    SMARTi SM00398. HMG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47095. SSF47095. 1 hit.
    PROSITEi PS50118. HMG_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Characterization of a high mobility group 1/2 homolog in yeast."
      Lu J., Kobayashi R., Brill S.J.
      J. Biol. Chem. 271:33678-33685(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    4. "Involvement of actin-related proteins in ATP-dependent chromatin remodeling."
      Shen X., Ranallo R., Choi E., Wu C.
      Mol. Cell 12:147-155(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE INO80 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNHP10_YEAST
    AccessioniPrimary (citable) accession number: Q03435
    Secondary accession number(s): D6VRY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1750 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3