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Protein

RNA polymerase sigma-54 factor

Gene

rpoN

Organism
Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. This sigma factor is required for the de novo biogenesis of both flagellum and stalk, and for polar morphogenesis and normal cell division. It may not be involved in nitrogen assimilation.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi380 – 399H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciCAULO:CC3599-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor
Gene namesi
Name:rpoN
Synonyms:flbU
Ordered Locus Names:CC_3599
OrganismiCaulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001816 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055271 – 497RNA polymerase sigma-54 factorAdd BLAST497

Proteomic databases

PRIDEiQ03408

Interactioni

Protein-protein interaction databases

STRINGi190650.CC_3599

Structurei

3D structure databases

ProteinModelPortaliQ03408
SMRiQ03408
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi471 – 479RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 29Gln-richAdd BLAST19

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DTW Bacteria
COG1508 LUCA
HOGENOMiHOG000071904
KOiK03092
OMAiVTTQKFM

Family and domain databases

Gene3Di1.10.10.1330, 1 hit
InterProiView protein in InterPro
IPR000394 RNA_pol_sigma_54
IPR007046 RNA_pol_sigma_54_core-bd
IPR007634 RNA_pol_sigma_54_DNA-bd
IPR038709 RpoN_core-bd_sf
PANTHERiPTHR32248 PTHR32248, 1 hit
PfamiView protein in Pfam
PF00309 Sigma54_AID, 1 hit
PF04963 Sigma54_CBD, 1 hit
PF04552 Sigma54_DBD, 1 hit
PIRSFiPIRSF000774 RpoN, 1 hit
PRINTSiPR00045 SIGMA54FCT
TIGRFAMsiTIGR02395 rpoN_sigma, 1 hit
PROSITEiView protein in PROSITE
PS00717 SIGMA54_1, 1 hit
PS00718 SIGMA54_2, 1 hit
PS50044 SIGMA54_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q03408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSHRLELR QGQGLVITPQ LQQAIKLLQL SNIELDAFVE AELERNPLLQ
60 70 80 90 100
RDEGDHEPAV EVEAERDASL TQVDAVADTT AGRELDTPVD DVSPGERATG
110 120 130 140 150
EGTDAEHAGG QIDWSRAGGG GSFESDEGYE RALTDSPTLA AHLRTQLVQA
160 170 180 190 200
ALSPAHNAIA EILIDAVDEG GYLRLDIVEL ADRLGCALAL VEETLSVLQG
210 220 230 240 250
FEPVGVFARD VRECLALQLK DLNRYDPAMA AMLDHLELLA KRDMAGLRRI
260 270 280 290 300
CGVDDEDLRE MIGEIRSLNP RPGAAYHSEP AETLVPDVMV REGLGGMWHV
310 320 330 340 350
ELNTDTLPRV LVDQRYHARV SKGARSDQEK TFVADCMASA NWLVKSLDQR
360 370 380 390 400
AKTILKVASE IVRQQDGFLA FGVEHLRPLN LKTVADAIGM HESTVSRVTS
410 420 430 440 450
NKYIATPRGV FELKFFFTSA IQSSEGGEAH SAASVRHKIK GLVDAEKCEA
460 470 480 490
DVHSDDRIVE ILKAAGVDIA RRTVAKYREA MRIPSSVERR RLMKEAV
Length:497
Mass (Da):54,457
Last modified:April 27, 2001 - v3
Checksum:i2F877D258FB984B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26K → M (PubMed:1459461).Curated1
Sequence conflicti26K → M (PubMed:9260957).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68549 Genomic DNA Translation: CAA48553.1
L11003 Genomic DNA Translation: AAA73956.1
AF006743 Genomic DNA Translation: AAC45594.1
AE005673 Genomic DNA Translation: AAK25561.1
PIRiS54743 I40668
RefSeqiNP_422393.1, NC_002696.2
WP_010921426.1, NC_002696.2

Genome annotation databases

EnsemblBacteriaiAAK25561; AAK25561; CC_3599
GeneIDi940711
KEGGiccr:CC_3599
PATRICifig|190650.5.peg.3602

Similar proteinsi

Entry informationi

Entry nameiRP54_CAUVC
AccessioniPrimary (citable) accession number: Q03408
Secondary accession number(s): O08456, O32343
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 27, 2001
Last modified: April 25, 2018
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health