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Protein

Serine/threonine-protein kinase PKH1

Gene

PKH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates YPK1 by phosphorylating of a threonine residue.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei154ATPBy similarity1
Binding sitei210ATPBy similarity1
Active sitei249Proton acceptorPROSITE-ProRule annotation1
Binding sitei253ATP; via carbonyl oxygenBy similarity1
Binding sitei267ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 137ATPBy similarity3
Nucleotide bindingi204 – 206ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • MAPK cascade involved in cell wall organization or biogenesis Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • peptidyl-threonine phosphorylation Source: SGD
  • positive regulation of cytoplasmic mRNA processing body assembly Source: SGD
  • positive regulation of protein serine/threonine kinase activity Source: SGD
  • regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30014-MONOMER
ReactomeiR-SCE-114604 GPVI-mediated activation cascade
R-SCE-1257604 PIP3 activates AKT signaling
R-SCE-165158 Activation of AKT2
R-SCE-202424 Downstream TCR signaling
R-SCE-2730905 Role of LAT2/NTAL/LAB on calcium mobilization
R-SCE-389357 CD28 dependent PI3K/Akt signaling
R-SCE-392451 G beta:gamma signalling through PI3Kgamma
R-SCE-444257 RSK activation
R-SCE-5218920 VEGFR2 mediated vascular permeability
R-SCE-5218921 VEGFR2 mediated cell proliferation
R-SCE-5625740 RHO GTPases activate PKNs
R-SCE-6804757 Regulation of TP53 Degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PKH1 (EC:2.7.11.1)
Alternative name(s):
3-phosphoinositide-dependent protein kinase 1
Gene namesi
Name:PKH1
Ordered Locus Names:YDR490C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR490C
SGDiS000002898 PKH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865521 – 766Serine/threonine-protein kinase PKH1Add BLAST766

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei294PhosphoserineCombined sources1
Modified residuei296PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03407
PaxDbiQ03407
PRIDEiQ03407

PTM databases

iPTMnetiQ03407

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32542, 138 interactors
DIPiDIP-1518N
ELMiQ03407
IntActiQ03407, 11 interactors
MINTiQ03407
STRINGi4932.YDR490C

Structurei

3D structure databases

ProteinModelPortaliQ03407
SMRiQ03407
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 391Protein kinasePROSITE-ProRule annotationAdd BLAST267

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 201PIF-pocketBy similarityAdd BLAST46

Domaini

The PIF-pocket is a small lobe in the catalytic domain required by the enzyme for the binding to the hydrophobic motif of its substrates. It is an allosteric regulatory site that can accommodate small compounds acting as allosteric inhibitors.By similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074819
InParanoidiQ03407
KOiK06276
OrthoDBiEOG092C0HH3

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q03407-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNRSLTEAD HALLSKPLVP TSAEHTQTQE YPRPFVDGSN SQSGSELQAS
60 70 80 90 100
PQGQFGEKAL TSTNRFIPLA NDDPGMQHEM GLDPSMRRRR EEWAERGAAK
110 120 130 140 150
IVKDVVDPAT GELTKHVVKM GIKDFKFGEQ LGDGSYSSVV LATARDSGKK
160 170 180 190 200
YAVKVLSKEY LIRQKKVKYV TVEKLALQKL NGTKGIFKLF FTFQDEASLY
210 220 230 240 250
FLLEYAPHGD FLGLIKKYGS LNETCARYYA SQIIDAVDSL HNIGIIHRDI
260 270 280 290 300
KPENILLDKN MKVKLTDFGT AKILPEEPSN TADGKPYFDL YAKSKSFVGT
310 320 330 340 350
AEYVSPELLN DNYTDSRCDI WAFGCILYQM LAGKPPFKAA NEYLTFQKVM
360 370 380 390 400
KIQYAFTAGF PQIVKDLVKK LLVRDPNDRL TIKQIKAHLF FHEVNFEDGS
410 420 430 440 450
VWDDNPPEIQ PYKINAEAMK PLQKVSESDT TVKMANLQLA GNGHADTPLQ
460 470 480 490 500
APAATSQEHS VISMTAATAA FNKDYTSQPK LGSKSSTSVR SASNNTDREV
510 520 530 540 550
IQKKVSKNRA SVSSPSISTT SRGKDNRSRS SDAFWSRYLQ NMDERVLLMK
560 570 580 590 600
EVALSTRNLE DSPVGLENVA LDYKNPLDIE PPTDSAGKFY KKMFLITNLG
610 620 630 640 650
RALVFVKRRS LSMWEEQEFE LQFELELNDV EKIRFISDQV LEIDGSRTIF
660 670 680 690 700
IGCKERAVLM KLWKLIHNGM TAKPKVVSPK SDHKMFDKFI LQKRQNTKKK
710 720 730 740 750
NQAPPVPQSN RLINGLPDRC ILKTPEEGAL HTKRPTSLQT RSSSNYSKLL
760
ARSTQMRKNM TRTDEK
Length:766
Mass (Da):86,253
Last modified:November 1, 1996 - v1
Checksum:i7FFD32E8BE8BC367
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA Translation: AAB64917.1
BK006938 Genomic DNA Translation: DAA12323.1
PIRiS69657
RefSeqiNP_010778.3, NM_001180798.3

Genome annotation databases

EnsemblFungiiYDR490C; YDR490C; YDR490C
GeneIDi852101
KEGGisce:YDR490C

Similar proteinsi

Entry informationi

Entry nameiPKH1_YEAST
AccessioniPrimary (citable) accession number: Q03407
Secondary accession number(s): D6VTB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Blood group antigen proteins
    Nomenclature of blood group antigens and list of entries
  2. Protein nomenclature information
    List of publications dealing with protein nomenclature and links to specialized websites

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