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Protein

DNA replication complex GINS protein SLD5

Gene

SLD5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for DNA replication. Functions as part of the GINS complex which plays an essential role in the initiation of DNA replication by binding to DNA replication origins and facilitating the assembly of the DNA replication machinery.By similarity1 Publication

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • DNA duplex unwinding Source: GOC
  • DNA replication initiation Source: GO_Central
  • double-strand break repair via break-induced replication Source: SGD

Keywordsi

Biological processDNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-30013-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein SLD5
Gene namesi
Name:SLD5Imported
Ordered Locus Names:YDR489W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR489W.
SGDiS000002897. SLD5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21S → P in sld5-8; temperature-sensitive mutant; in association with P-66. Defective in DNA replication. 1 Publication1
Mutagenesisi66S → P in sld5-8; temperature-sensitive mutant; in association with P-21. Defective in DNA replication. 1 Publication1
Mutagenesisi67W → R in sld5-12; temperature-sensitive mutant. Defective in DNA replication. 1 Publication1
Mutagenesisi150K → E in sld5-2; temperature-sensitive mutant. Defective in DNA replication. 1 Publication1
Mutagenesisi293L → P in sld5-13; temperature-sensitive mutant. Defective in DNA replication. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000719551 – 294DNA replication complex GINS protein SLD5Add BLAST294

Proteomic databases

MaxQBiQ03406.
PRIDEiQ03406.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer composed of SLD5, PSF1, PSF2 and PSF3.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32541. 165 interactors.
DIPiDIP-1813N.
IntActiQ03406. 33 interactors.
MINTiMINT-409410.
STRINGi4932.YDR489W.

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 14Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JC5electron microscopy4.70D1-294[»]
3JC6electron microscopy3.70D1-294[»]
3JC7electron microscopy4.80D1-294[»]
4C95X-ray2.69D/E1-19[»]
5U8Selectron microscopy6.10D1-294[»]
5U8Telectron microscopy4.90D1-294[»]
ProteinModelPortaliQ03406.
SMRiQ03406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GINS4/SLD5 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003246.
HOGENOMiHOG000215319.
InParanoidiQ03406.
KOiK10735.
OMAiVSIHRME.
OrthoDBiEOG092C50L6.

Family and domain databases

InterProiView protein in InterPro
IPR021151. GINS_A.
IPR008591. GINS_Sld5.
IPR031633. SLD5_C.
PANTHERiPTHR21206. PTHR21206. 1 hit.
PfamiView protein in Pfam
PF05916. Sld5. 1 hit.
PF16922. SLD5_C. 1 hit.
PIRSFiPIRSF007764. Sld5. 1 hit.
SUPFAMiSSF158573. SSF158573. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDINIDDILA ELDKETTAVD STKITQGSSS TTHRDANTIV GSSLDLNDKT
60 70 80 90 100
QIYVSPQQDF SDLMKSWKNE RCSPELLPYP HQLMKRLLNR ISMQSQLIEN
110 120 130 140 150
ISMGFLDMQN ASNANPPMPN ESKLPLLCME TELERLKFVI RSYIRCRLSK
160 170 180 190 200
IDKFSLYLRQ LNEDENSLIS LTDLLSKDEI KYHDTHSLIW LKLVNDSILK
210 220 230 240 250
YMPEELQAIN DTEGSVNMID EPDWNKFVFI HVNGPPDGKW NEDPLLQENE
260 270 280 290
FGKPCYTVTI PDLKEEVELT IGSIYVMRYE VIRDLLRDDK VALI
Length:294
Mass (Da):33,948
Last modified:November 1, 1996 - v1
Checksum:i7EC516ADAD9900DD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232V → A in AAS56818 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64907.1.
AY558492 Genomic DNA. Translation: AAS56818.1.
BK006938 Genomic DNA. Translation: DAA12322.1.
PIRiS69656.
RefSeqiNP_010777.3. NM_001180797.3.

Genome annotation databases

EnsemblFungiiYDR489W; YDR489W; YDR489W.
GeneIDi852100.
KEGGisce:YDR489W.

Similar proteinsi

Entry informationi

Entry nameiSLD5_YEAST
AccessioniPrimary (citable) accession number: Q03406
Secondary accession number(s): D6VTB2, Q6Q558
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names