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Protein

DNA replication complex GINS protein SLD5

Gene

SLD5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for DNA replication. Functions as part of the GINS complex which plays an essential role in the initiation of DNA replication by binding to DNA replication origins and facilitating the assembly of the DNA replication machinery.By similarity1 Publication

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-30013-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein SLD5
Gene namesi
Name:SLD5Imported
Ordered Locus Names:YDR489W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR489W.
SGDiS000002897. SLD5.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • DNA replication preinitiation complex Source: SGD
  • GINS complex Source: SGD
  • replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi21 – 211S → P in sld5-8; temperature-sensitive mutant; in association with P-66. Defective in DNA replication. 1 Publication
Mutagenesisi66 – 661S → P in sld5-8; temperature-sensitive mutant; in association with P-21. Defective in DNA replication. 1 Publication
Mutagenesisi67 – 671W → R in sld5-12; temperature-sensitive mutant. Defective in DNA replication. 1 Publication
Mutagenesisi150 – 1501K → E in sld5-2; temperature-sensitive mutant. Defective in DNA replication. 1 Publication
Mutagenesisi293 – 2931L → P in sld5-13; temperature-sensitive mutant. Defective in DNA replication. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294DNA replication complex GINS protein SLD5PRO_0000071955Add
BLAST

Proteomic databases

MaxQBiQ03406.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer composed of SLD5, PSF1, PSF2 and PSF3.

Binary interactionsi

WithEntry#Exp.IntActNotes
PSF1Q124884EBI-37437,EBI-22066
PSF2P403598EBI-37437,EBI-25936

Protein-protein interaction databases

BioGridi32541. 40 interactions.
DIPiDIP-1813N.
IntActiQ03406. 27 interactions.
MINTiMINT-409410.

Structurei

Secondary structure

1
294
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 149Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JC5electron microscopy4.70D1-294[»]
3JC6electron microscopy3.70D1-294[»]
3JC7electron microscopy4.80D1-294[»]
4C95X-ray2.69D/E1-19[»]
ProteinModelPortaliQ03406.
SMRiQ03406. Positions 54-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GINS4/SLD5 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003246.
HOGENOMiHOG000215319.
InParanoidiQ03406.
KOiK10735.
OMAiPLLCMEA.
OrthoDBiEOG7KDFPN.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR008591. GINS_Sld5.
IPR031633. SLD5_C.
[Graphical view]
PANTHERiPTHR21206. PTHR21206. 2 hits.
PfamiPF05916. Sld5. 1 hit.
PF16922. SLD5_C. 1 hit.
[Graphical view]
PIRSFiPIRSF007764. Sld5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDINIDDILA ELDKETTAVD STKITQGSSS TTHRDANTIV GSSLDLNDKT
60 70 80 90 100
QIYVSPQQDF SDLMKSWKNE RCSPELLPYP HQLMKRLLNR ISMQSQLIEN
110 120 130 140 150
ISMGFLDMQN ASNANPPMPN ESKLPLLCME TELERLKFVI RSYIRCRLSK
160 170 180 190 200
IDKFSLYLRQ LNEDENSLIS LTDLLSKDEI KYHDTHSLIW LKLVNDSILK
210 220 230 240 250
YMPEELQAIN DTEGSVNMID EPDWNKFVFI HVNGPPDGKW NEDPLLQENE
260 270 280 290
FGKPCYTVTI PDLKEEVELT IGSIYVMRYE VIRDLLRDDK VALI
Length:294
Mass (Da):33,948
Last modified:November 1, 1996 - v1
Checksum:i7EC516ADAD9900DD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2321V → A in AAS56818 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64907.1.
AY558492 Genomic DNA. Translation: AAS56818.1.
BK006938 Genomic DNA. Translation: DAA12322.1.
PIRiS69656.
RefSeqiNP_010777.3. NM_001180797.3.

Genome annotation databases

EnsemblFungiiYDR489W; YDR489W; YDR489W.
GeneIDi852100.
KEGGisce:YDR489W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64907.1.
AY558492 Genomic DNA. Translation: AAS56818.1.
BK006938 Genomic DNA. Translation: DAA12322.1.
PIRiS69656.
RefSeqiNP_010777.3. NM_001180797.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JC5electron microscopy4.70D1-294[»]
3JC6electron microscopy3.70D1-294[»]
3JC7electron microscopy4.80D1-294[»]
4C95X-ray2.69D/E1-19[»]
ProteinModelPortaliQ03406.
SMRiQ03406. Positions 54-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32541. 40 interactions.
DIPiDIP-1813N.
IntActiQ03406. 27 interactions.
MINTiMINT-409410.

Proteomic databases

MaxQBiQ03406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR489W; YDR489W; YDR489W.
GeneIDi852100.
KEGGisce:YDR489W.

Organism-specific databases

EuPathDBiFungiDB:YDR489W.
SGDiS000002897. SLD5.

Phylogenomic databases

GeneTreeiENSGT00390000003246.
HOGENOMiHOG000215319.
InParanoidiQ03406.
KOiK10735.
OMAiPLLCMEA.
OrthoDBiEOG7KDFPN.

Enzyme and pathway databases

BioCyciYEAST:G3O-30013-MONOMER.

Miscellaneous databases

PROiQ03406.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR008591. GINS_Sld5.
IPR031633. SLD5_C.
[Graphical view]
PANTHERiPTHR21206. PTHR21206. 2 hits.
PfamiPF05916. Sld5. 1 hit.
PF16922. SLD5_C. 1 hit.
[Graphical view]
PIRSFiPIRSF007764. Sld5. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
    Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
    Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COMPOSITION OF THE GINS COMPLEX, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-21; SER-66; TRP-67; LYS-150 AND LEU-293.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiSLD5_YEAST
AccessioniPrimary (citable) accession number: Q03406
Secondary accession number(s): D6VTB2, Q6Q558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.