Reviewed, UniProtKB/Swiss-Prot Q033G3 (MTLD_LACLS)

Names and origin

Protein names

Recommended name:
    Mannitol-1-phosphate 5-dehydrogenase
    EC=1.1.1.17

Gene names

Name:	mtlD
Ordered Locus Names:	LACR_0030

Organism

Lactococcus lactis subsp. cremoris (strain SK11) [Complete proteome] [HAMAP]

Taxonomic identifier

272622 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH. HAMAP MF_00196
Sequence similarities	Belongs to the mannitol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro mannitol-1-phosphate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Mannitol-1-phosphate 5-dehydrogenase HAMAP MF_00196		PRO_1000011804
Regions
	Nucleotide binding	4 – 15	12	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q033G3-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 2ABC9359F30FB71D	FASTA	388	43,993
	10 20 30 40 50 60 MKKAVHFGAG NIGRGFIGEI LSKNGFEIYF VDTNKAIIDE LNTRHSYEIG IASSSPEKIS 70 80 90 100 110 120 VSGLFGINNG ENPEDVIEAI AQADIVTKAI GPNILPYIAE LVAKGIQKRK EENKQVQIDI 130 140 150 160 170 180 IACENMIGGS EFLEKKVAEY LSDSDKVYLA NYIGFPNAAV DRIVPGQKHE DLLYVEVEPF 190 200 210 220 230 240 CEWVIDESQI KNKSFKLEGV HYANNLEPFI ERKLFSVNSG HATVAYSSAY KGYKIILEGL 250 260 270 280 290 300 QHKEILSALK GVQKETRALL LAKWPQYFTE EDLMSYHQMI ISRFANPKII DEVTRVARTP 310 320 330 340 350 360 IRKLGYDERF IRPIRELNER GLSYQNHLDI VGKIFAYQDE NDSQSVQLQE KLSTMDLQRL 370 380 IEEVTGLSNK KIILEIELVI KKYKNDSK

References

[1]

"Comparative genomics of the lactic acid bacteria." Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V., Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V., Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M., Hawkins T. , Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A., Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W., Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y., Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R., O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T., Richardson P., Kozyavkin S., Weimer B.C., Mills D.A. Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006) [PubMed: 17030793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000425 Genomic DNA. Translation: ABJ71659.1.
RefSeq	YP_808081.1.
3D structure databases
SMR	Q033G3. Positions 3-337.
ModBase	Search...
Protein-protein interaction databases
STRING	Q033G3.
Genome annotation databases
GeneID	4433611.
GenomeReviews	Gene locus LACR_0030 in contig CP000425_GR.
KEGG	llc:LACR_0030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0246.
HOGENOM	HBG568825.
OMA	VDRIVPN.
Family and domain databases
HAMAP	MF_00196. Mannitol_dehydrog. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR013118. Mannitol_DH_C. IPR000669. Mannitol_DH_core. IPR013131. Mannitol_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
PROSITE	PS00974. MANNITOL_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTLD_LACLS

Accession

Primary (citable) accession number: Q033G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)