Reviewed, UniProtKB/Swiss-Prot Q03392 (PCNA_SCHPO)

Names and origin

Protein names

Recommended name:
    Proliferating cell nuclear antigen
      Short name=PCNA

Gene names

Name:	pcn1
Synonyms:	pcn
ORF Names:	SPBC16D10.09

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
Subunit structure	Interacts with cdc24 and rfc1. Ref.3
Subcellular location	Nucleus.
Sequence similarities	Belongs to the PCNA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA strand elongation during DNA replication Inferred by curator. Source: GeneDB_SPombe cellular response to UV Inferred from mutant phenotype. Source: GeneDB_SPombe postreplication repair Inferred from genetic interaction. Source: GeneDB_SPombe regulation of DNA replication Inferred from electronic annotation. Source: InterPro regulation of cell cycle Inferred from mutant phenotype. Source: GeneDB_SPombe response to ionizing radiation Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	PCNA complex Inferred from mutant phenotype. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe nuclear replication fork Inferred by curator. Source: GeneDB_SPombe
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA polymerase processivity factor activity Inferred from electronic annotation. Source: InterPro protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cdc24	O75004	1	EBI-768724,EBI-1559355
cdc27	P30261	3	EBI-768724,EBI-866919
CDKN1A	P38936	1	EBI-768724,EBI-375077	From a different organism.
myh1	Q10159	1	EBI-768724,EBI-767574
POL32	P47110	1	EBI-768724,EBI-6084	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Proliferating cell nuclear antigen		PRO_0000149175
Regions
	DNA binding	61 – 80	20	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03392-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1EE5B2A27A899C4E	FASTA	260	28,969
	10 20 30 40 50 60 MLEARFQQAA LLKKLLDAIK ELVTDANFDC NDNGISLQAM DSSHVALVSM LIKSDGFEPY 70 80 90 100 110 120 RCDRNIALGI NLNALSKVLR CAQNEDLVTL KAEDTPEVLN LVFESEKNDR ISDYDVKLMD 130 140 150 160 170 180 IDQEHLGIPD IEYDATITMP AAEFQRITRD LLTLSDSVTI NASKEGVRFS CKGDIGNGST 190 200 210 220 230 240 TLKQHTDLSD QDQSIEISLT QAVTLTFSLK YLAQFTKATP LATRVTLSMS NDVPLLVEYK 250 260 MESGFLRFYL APKIGEEDEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and analysis of the fission yeast gene encoding polymerase delta accessory protein PCNA." Waseem N.H., Labib K., Nurse P., Lane D.P. EMBO J. 11:5111-5120(1992) [PubMed: 1361173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion." Tanaka H., Tanaka K., Murakami H., Okayama H. Mol. Cell. Biol. 19:1038-1048(1999) [PubMed: 9891039] [Abstract] Cited for: INTERACTION WITH CDC24 AND RFC1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54857 Genomic DNA. Translation: CAA38636.1. CU329671 Genomic DNA. Translation: CAB38513.1.
PIR	WMZPET. S28053.
RefSeq	NP_596504.1.
3D structure databases
SMR	Q03392. Positions 1-258.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03392. 9 interactions.
STRING	Q03392.
Genome annotation databases
GeneID	2540020.
GenomeReviews	Gene locus pcn1 in contig CU329671_GR.
KEGG	spo:SPBC16D10.09.
NMPDR	fig|4896.1.peg.2370.
Organism-specific databases
GeneDB_Spombe	SPBC16D10.09.
Phylogenomic databases
eggNOG	fuNOG07950.
HOGENOM	HBG749295.
OMA	KEEEAXN.
OrthoDB	EOG9X0P8S.
PhylomeDB	Q03392.
Gene expression databases
ArrayExpress	Q03392.
Family and domain databases
InterPro	IPR000730. Pr_cel_nuc_antig. [Graphical view]
PANTHER	PTHR11352. Pr_cel_nuc_antig. 1 hit.
Pfam	PF02747. PCNA_C. 1 hit. PF00705. PCNA_N. 1 hit. [Graphical view]
PRINTS	PR00339. PCNACYCLIN.
TIGRFAMs	TIGR00590. pcna. 1 hit.
PROSITE	PS01251. PCNA_1. 1 hit. PS00293. PCNA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCNA_SCHPO

Accession

Primary (citable) accession number: Q03392

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	February 9, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)