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Q03391

- NMDE4_MOUSE

UniProt

Q03391 - NMDE4_MOUSE

Protein

Glutamate receptor ionotropic, NMDA 2D

Gene

Grin2d

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei639 – 6391Functional determinant of NMDA receptorsBy similarity

    GO - Molecular functioni

    1. extracellular-glutamate-gated ion channel activity Source: RefGenome
    2. N-methyl-D-aspartate selective glutamate receptor activity Source: MGI

    GO - Biological processi

    1. adult locomotory behavior Source: MGI
    2. ionotropic glutamate receptor signaling pathway Source: GOC
    3. ion transmembrane transport Source: GOC
    4. regulation of sensory perception of pain Source: MGI
    5. startle response Source: MGI
    6. synaptic transmission, glutamatergic Source: RefGenome

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Calcium, Magnesium

    Enzyme and pathway databases

    ReactomeiREACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
    REACT_198248. CREB phosphorylation through the activation of CaMKII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate receptor ionotropic, NMDA 2D
    Short name:
    GluN2D
    Alternative name(s):
    Glutamate [NMDA] receptor subunit epsilon-4
    N-methyl D-aspartate receptor subtype 2D
    Short name:
    NMDAR2D
    Short name:
    NR2D
    Gene namesi
    Name:Grin2d
    Synonyms:GluN2D
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:95823. Grin2d.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. dendrite Source: RefGenome
    3. N-methyl-D-aspartate selective glutamate receptor complex Source: RefGenome
    4. postsynaptic membrane Source: RefGenome
    5. synapse Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 13231296Glutamate receptor ionotropic, NMDA 2DPRO_0000011584Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi363 – 3631N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi452 ↔ 480By similarity
    Disulfide bondi459 ↔ 481By similarity
    Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi566 – 5661N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi770 ↔ 825By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ03391.
    PRIDEiQ03391.

    PTM databases

    PhosphoSiteiQ03391.

    Expressioni

    Gene expression databases

    ArrayExpressiQ03391.
    BgeeiQ03391.
    GenevestigatoriQ03391.

    Interactioni

    Subunit structurei

    Interacts with PDZ domains of INADL and DLG4 By similarity. Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B).By similarity

    Protein-protein interaction databases

    BioGridi200071. 2 interactions.
    IntActiQ03391. 2 interactions.
    MINTiMINT-4103932.
    STRINGi10090.ENSMUSP00000002848.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03391.
    SMRiQ03391. Positions 426-564, 686-826.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 580553ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini602 – 65352CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini675 – 841167ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini863 – 1323461CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei581 – 60121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei654 – 67421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei842 – 86221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1321 – 13233PDZ-bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi278 – 2836Poly-Gly
    Compositional biasi905 – 9139Poly-Pro
    Compositional biasi1030 – 10356Poly-Ala
    Compositional biasi1197 – 12015Poly-Pro

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG282132.
    GeneTreeiENSGT00540000070221.
    HOGENOMiHOG000113803.
    HOVERGENiHBG052637.
    InParanoidiQ03391.
    KOiK05212.
    OMAiWDYLPPR.
    OrthoDBiEOG72ZCD1.
    TreeFamiTF314731.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF10613. Lig_chan-Glu_bd. 1 hit.
    [Graphical view]
    PRINTSiPR00177. NMDARECEPTOR.
    SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03391-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGAGGPRGP RGPAKMLLLL ALACASPFPE EVPGPGAAGG GTGGARPLNV     50
    ALVFSGPAYA AEAARLGPAV AAAVRSPGLD VRPVALVLNG SDPRSLVLQL 100
    CDLLSGLRVH GVVFEDDSRA PAVAPILDFL SAQTSLPIVA VHGGAALVLT 150
    PKEKGSTFLQ LGSSTEQQLQ VIFEVLEEYD WTSFVAVTTR APGHRAFLSY 200
    IEVLTDGSLV GWEHRGALTL DPGAGEAVLG AQLRSVSAQI RLLFCAREEA 250
    EPVFRAAEEA GLTGPGYVWF MVGPQLAGGG GSGVPGEPLL LPGGAPLPAG 300
    LFAVRSAGWR DDLARRVAAG VAVVARGAQA LLRDYGFLPE LGHDCRAQNR 350
    THRGESLHRY FMNITWDNRD YSFNEDGFLV NPSLVVISLT RDRTWEVVGS 400
    WEQQTLRLKY PLWSRYGRFL QPVDDTQHLT VATLEERPFV IVEPADPISG 450
    TCIRDSVPCR SQLNRTHSPP PDAPRPEKRC CKGFCIDILK RLAHTIGFSY 500
    DLYLVTNGKH GKKIDGVWNG MIGEVFYQRA DMAIGSLTIN EERSEIVDFS 550
    VPFVETGISV MVARSNGTVS PSAFLEPYSP AVWVMMFVMC LTVVAVTVFI 600
    FEYLSPVGYN RSLATGKRPG GSTFTIGKSI WLLWALVFNN SVPVENPRGT 650
    TSKIMVLVWA FFAVIFLASY TANLAAFMIQ EEYVDTVSGL SDRKFQRPQE 700
    QYPPLKFGTV PNGSTEKNIR SNYPDMHSYM VRYNQPRVEE ALTQLKAGKL 750
    DAFIYDAAVL NYMARKDEGC KLVTIGSGKV FATTGYGIAL HKGSRWKRPI 800
    DLALLQFLGD DEIEMLERLW LSGICHNDKI EVMSSKLDID NMAGVFYMLL 850
    VAMGLSLLVF AWEHLVYWRL RHCLGPTHRM DFLLAFSRGM YSCCSAEAAP 900
    PPAKPPPPPQ PLPSPAYPAA RPPPGPAPFV PRERAAADRW RRAKGTGPPG 950
    GAALADGFHR YYGPIEPQGL GLGEARAAPR GAAGRPLSPP TTQPPQKPPP 1000
    SYFAIVREQE PAEPPAGAFP GFPSPPAPPA AAAAAVGPPL CRLAFEDESP 1050
    PAPSRWPRSD PESQPLLGGG AGGPSAGAPT APPPRRAAPP PCAYLDLEPS 1100
    PSDSEDSESL GGASLGGLEP WWFADFPYPY AERLGPPPGR YWSVDKLGGW 1150
    RAGSWDYLPP RGGPAWHCRH CASLELLPPP RHLSCSHDGL DGGWWAPPPP 1200
    PWAAGPPPRR RARCGCPRPH PHRPRASHRA PAAAPHHHRH RRAAGGWDLP 1250
    PPAPTSRSLE DLSSCPRAAP TRRLTGPSRH ARRCPHAAHW GPPLPTASHR 1300
    RHRGGDLGTR RGSAHFSSLE SEV 1323
    Length:1,323
    Mass (Da):142,963
    Last modified:July 27, 2011 - v3
    Checksum:i3DF8860D19677586
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1087 – 10871A → T in BAA02254. (PubMed:1385220)Curated
    Sequence conflicti1208 – 12092PR → AP in BAA02254. (PubMed:1385220)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB154245 mRNA. Translation: BAI44630.1.
    D12822 mRNA. Translation: BAA02254.1.
    CCDSiCCDS21267.1.
    PIRiS27224.
    RefSeqiNP_032198.2. NM_008172.2.
    XP_006540695.1. XM_006540632.1.
    UniGeneiMm.322594.

    Genome annotation databases

    EnsembliENSMUST00000002848; ENSMUSP00000002848; ENSMUSG00000002771.
    GeneIDi14814.
    KEGGimmu:14814.
    UCSCiuc009gxm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB154245 mRNA. Translation: BAI44630.1 .
    D12822 mRNA. Translation: BAA02254.1 .
    CCDSi CCDS21267.1.
    PIRi S27224.
    RefSeqi NP_032198.2. NM_008172.2.
    XP_006540695.1. XM_006540632.1.
    UniGenei Mm.322594.

    3D structure databases

    ProteinModelPortali Q03391.
    SMRi Q03391. Positions 426-564, 686-826.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200071. 2 interactions.
    IntActi Q03391. 2 interactions.
    MINTi MINT-4103932.
    STRINGi 10090.ENSMUSP00000002848.

    Chemistry

    BindingDBi Q03391.
    GuidetoPHARMACOLOGYi 459.

    PTM databases

    PhosphoSitei Q03391.

    Proteomic databases

    PaxDbi Q03391.
    PRIDEi Q03391.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002848 ; ENSMUSP00000002848 ; ENSMUSG00000002771 .
    GeneIDi 14814.
    KEGGi mmu:14814.
    UCSCi uc009gxm.1. mouse.

    Organism-specific databases

    CTDi 2906.
    MGIi MGI:95823. Grin2d.

    Phylogenomic databases

    eggNOGi NOG282132.
    GeneTreei ENSGT00540000070221.
    HOGENOMi HOG000113803.
    HOVERGENi HBG052637.
    InParanoidi Q03391.
    KOi K05212.
    OMAi WDYLPPR.
    OrthoDBi EOG72ZCD1.
    TreeFami TF314731.

    Enzyme and pathway databases

    Reactomei REACT_198244. Ras activation uopn Ca2+ infux through NMDA receptor.
    REACT_198248. CREB phosphorylation through the activation of CaMKII.

    Miscellaneous databases

    NextBioi 287011.
    PROi Q03391.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q03391.
    Bgeei Q03391.
    Genevestigatori Q03391.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF10613. Lig_chan-Glu_bd. 1 hit.
    [Graphical view ]
    PRINTSi PR00177. NMDARECEPTOR.
    SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression of mRNA in mouse spinal cord."
      Nishizwa M., Ito S.
      Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ddY.
      Tissue: Spinal cord.
    2. "Cloning and expression of the epsilon 4 subunit of the NMDA receptor channel."
      Ikeda K., Nagasawa M., Mori H., Araki K., Sakimura K., Watanabe M., Inoue Y., Mishina M.
      FEBS Lett. 313:34-38(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. Ikeda K., Nagasawa M., Mori H., Araki K., Sakimura K., Watanabe M., Inoue Y., Mishina M.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.

    Entry informationi

    Entry nameiNMDE4_MOUSE
    AccessioniPrimary (citable) accession number: Q03391
    Secondary accession number(s): C9K0Z5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3