Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q03391 (NMDE4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate receptor ionotropic, NMDA 2D

Short name=GluN2D
Alternative name(s):
Glutamate [NMDA] receptor subunit epsilon-4
N-methyl D-aspartate receptor subtype 2D
Short name=NMDAR2D
Short name=NR2D
Gene names
Name:Grin2d
Synonyms:GluN2D
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1323 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Subunit structure

Interacts with PDZ domains of INADL and DLG4 By similarity. Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B).

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 13231296Glutamate receptor ionotropic, NMDA 2D
PRO_0000011584

Regions

Topological domain28 – 580553Extracellular Potential
Transmembrane581 – 60121Helical; Potential
Topological domain602 – 65352Cytoplasmic Potential
Transmembrane654 – 67421Helical; Potential
Topological domain675 – 841167Extracellular Potential
Transmembrane842 – 86221Helical; Potential
Topological domain863 – 1323461Cytoplasmic Potential
Motif1321 – 13233PDZ-binding By similarity
Compositional bias278 – 2836Poly-Gly
Compositional bias905 – 9139Poly-Pro
Compositional bias1030 – 10356Poly-Ala
Compositional bias1197 – 12015Poly-Pro

Sites

Site6391Functional determinant of NMDA receptors By similarity

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation3491N-linked (GlcNAc...) Potential
Glycosylation3631N-linked (GlcNAc...) Potential
Glycosylation4641N-linked (GlcNAc...) Potential
Glycosylation5661N-linked (GlcNAc...) Potential
Disulfide bond452 ↔ 480 By similarity
Disulfide bond459 ↔ 481 By similarity
Disulfide bond770 ↔ 825 By similarity

Experimental info

Sequence conflict10871A → T in BAA02254. Ref.2
Sequence conflict1208 – 12092PR → AP in BAA02254. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q03391 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 3DF8860D19677586

FASTA1,323142,963
        10         20         30         40         50         60 
MRGAGGPRGP RGPAKMLLLL ALACASPFPE EVPGPGAAGG GTGGARPLNV ALVFSGPAYA 

        70         80         90        100        110        120 
AEAARLGPAV AAAVRSPGLD VRPVALVLNG SDPRSLVLQL CDLLSGLRVH GVVFEDDSRA 

       130        140        150        160        170        180 
PAVAPILDFL SAQTSLPIVA VHGGAALVLT PKEKGSTFLQ LGSSTEQQLQ VIFEVLEEYD 

       190        200        210        220        230        240 
WTSFVAVTTR APGHRAFLSY IEVLTDGSLV GWEHRGALTL DPGAGEAVLG AQLRSVSAQI 

       250        260        270        280        290        300 
RLLFCAREEA EPVFRAAEEA GLTGPGYVWF MVGPQLAGGG GSGVPGEPLL LPGGAPLPAG 

       310        320        330        340        350        360 
LFAVRSAGWR DDLARRVAAG VAVVARGAQA LLRDYGFLPE LGHDCRAQNR THRGESLHRY 

       370        380        390        400        410        420 
FMNITWDNRD YSFNEDGFLV NPSLVVISLT RDRTWEVVGS WEQQTLRLKY PLWSRYGRFL 

       430        440        450        460        470        480 
QPVDDTQHLT VATLEERPFV IVEPADPISG TCIRDSVPCR SQLNRTHSPP PDAPRPEKRC 

       490        500        510        520        530        540 
CKGFCIDILK RLAHTIGFSY DLYLVTNGKH GKKIDGVWNG MIGEVFYQRA DMAIGSLTIN 

       550        560        570        580        590        600 
EERSEIVDFS VPFVETGISV MVARSNGTVS PSAFLEPYSP AVWVMMFVMC LTVVAVTVFI 

       610        620        630        640        650        660 
FEYLSPVGYN RSLATGKRPG GSTFTIGKSI WLLWALVFNN SVPVENPRGT TSKIMVLVWA 

       670        680        690        700        710        720 
FFAVIFLASY TANLAAFMIQ EEYVDTVSGL SDRKFQRPQE QYPPLKFGTV PNGSTEKNIR 

       730        740        750        760        770        780 
SNYPDMHSYM VRYNQPRVEE ALTQLKAGKL DAFIYDAAVL NYMARKDEGC KLVTIGSGKV 

       790        800        810        820        830        840 
FATTGYGIAL HKGSRWKRPI DLALLQFLGD DEIEMLERLW LSGICHNDKI EVMSSKLDID 

       850        860        870        880        890        900 
NMAGVFYMLL VAMGLSLLVF AWEHLVYWRL RHCLGPTHRM DFLLAFSRGM YSCCSAEAAP 

       910        920        930        940        950        960 
PPAKPPPPPQ PLPSPAYPAA RPPPGPAPFV PRERAAADRW RRAKGTGPPG GAALADGFHR 

       970        980        990       1000       1010       1020 
YYGPIEPQGL GLGEARAAPR GAAGRPLSPP TTQPPQKPPP SYFAIVREQE PAEPPAGAFP 

      1030       1040       1050       1060       1070       1080 
GFPSPPAPPA AAAAAVGPPL CRLAFEDESP PAPSRWPRSD PESQPLLGGG AGGPSAGAPT 

      1090       1100       1110       1120       1130       1140 
APPPRRAAPP PCAYLDLEPS PSDSEDSESL GGASLGGLEP WWFADFPYPY AERLGPPPGR 

      1150       1160       1170       1180       1190       1200 
YWSVDKLGGW RAGSWDYLPP RGGPAWHCRH CASLELLPPP RHLSCSHDGL DGGWWAPPPP 

      1210       1220       1230       1240       1250       1260 
PWAAGPPPRR RARCGCPRPH PHRPRASHRA PAAAPHHHRH RRAAGGWDLP PPAPTSRSLE 

      1270       1280       1290       1300       1310       1320 
DLSSCPRAAP TRRLTGPSRH ARRCPHAAHW GPPLPTASHR RHRGGDLGTR RGSAHFSSLE 


SEV 

« Hide

References

[1]"Expression of mRNA in mouse spinal cord."
Nishizwa M., Ito S.
Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ddY.
Tissue: Spinal cord.
[2]"Cloning and expression of the epsilon 4 subunit of the NMDA receptor channel."
Ikeda K., Nagasawa M., Mori H., Araki K., Sakimura K., Watanabe M., Inoue Y., Mishina M.
FEBS Lett. 313:34-38(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]Ikeda K., Nagasawa M., Mori H., Araki K., Sakimura K., Watanabe M., Inoue Y., Mishina M.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB154245 mRNA. Translation: BAI44630.1.
D12822 mRNA. Translation: BAA02254.1.
PIRS27224.
RefSeqNP_032198.2. NM_008172.2.
XP_006540695.1. XM_006540632.1.
UniGeneMm.322594.

3D structure databases

ProteinModelPortalQ03391.
SMRQ03391. Positions 426-564, 686-826.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200071. 2 interactions.
IntActQ03391. 2 interactions.
MINTMINT-4103932.
STRING10090.ENSMUSP00000002848.

Chemistry

BindingDBQ03391.

PTM databases

PhosphoSiteQ03391.

Proteomic databases

PaxDbQ03391.
PRIDEQ03391.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002848; ENSMUSP00000002848; ENSMUSG00000002771.
GeneID14814.
KEGGmmu:14814.
UCSCuc009gxm.1. mouse.

Organism-specific databases

CTD2906.
MGIMGI:95823. Grin2d.

Phylogenomic databases

eggNOGNOG282132.
GeneTreeENSGT00540000070221.
HOGENOMHOG000113803.
HOVERGENHBG052637.
InParanoidQ03391.
KOK05212.
OMAWDYLPPR.
OrthoDBEOG72ZCD1.
TreeFamTF314731.

Gene expression databases

ArrayExpressQ03391.
BgeeQ03391.
GenevestigatorQ03391.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSPR00177. NMDARECEPTOR.
SMARTSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
ProtoNetSearch...

Other

NextBio287011.
PROQ03391.
SOURCESearch...

Entry information

Entry nameNMDE4_MOUSE
AccessionPrimary (citable) accession number: Q03391
Secondary accession number(s): C9K0Z5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot