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Protein

Glutamate receptor ionotropic, NMDA 2D

Gene

Grin2d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+ (PubMed:1385220). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable).Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei538GlutamateBy similarity1
Binding sitei543GlutamateBy similarity1
Sitei639Functional determinant of NMDA receptorsBy similarity1
Binding sitei756GlutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adult locomotory behavior Source: MGI
  • calcium ion transmembrane import into cytosol Source: UniProtKB
  • regulation of sensory perception of pain Source: MGI
  • startle response Source: MGI

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandCalcium, Magnesium

Enzyme and pathway databases

ReactomeiR-MMU-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-MMU-442729. CREB phosphorylation through the activation of CaMKII.
R-MMU-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-MMU-5673001. RAF/MAP kinase cascade.
R-MMU-8849932. Synaptic adhesion-like molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 2D
Short name:
GluN2D
Alternative name(s):
Glutamate [NMDA] receptor subunit epsilon-42 Publications
N-methyl D-aspartate receptor subtype 2D
Short name:
NMDAR2D
Short name:
NR2D
Gene namesi
Name:Grin2d
Synonyms:GluN2D
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95823. Grin2d.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 581ExtracellularCuratedAdd BLAST554
Transmembranei582 – 600HelicalBy similarityAdd BLAST19
Topological domaini601 – 627CytoplasmicCuratedAdd BLAST27
Intramembranei628 – 647Discontinuously helicalBy similarityAdd BLAST20
Topological domaini648 – 654CytoplasmicCurated7
Transmembranei655 – 670HelicalBy similarityAdd BLAST16
Topological domaini671 – 841ExtracellularCuratedAdd BLAST171
Transmembranei842 – 861HelicalBy similarityAdd BLAST20
Topological domaini862 – 1323CytoplasmicCuratedAdd BLAST462

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate, are viable and fertile. Their brains appear grossly normal. Mutant mice show reduced spontaneous locomotion activity, but have no visible impairment of motor skills. They have normal exploratory behavior, but their behavior in a new situation suggests increased novelty preference.1 Publication

Chemistry databases

ChEMBLiCHEMBL3832634.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001158428 – 1323Glutamate receptor ionotropic, NMDA 2DAdd BLAST1296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi101 ↔ 345By similarity
Glycosylationi349N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi363N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi452 ↔ 480By similarity
Disulfide bondi459 ↔ 481By similarity
Glycosylationi464N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi566N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi770 ↔ 825By similarity
Modified residuei1303Omega-N-methylarginineCombined sources1
Modified residuei1313PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ03391.
PeptideAtlasiQ03391.
PRIDEiQ03391.

PTM databases

iPTMnetiQ03391.
PhosphoSitePlusiQ03391.

Expressioni

Tissue specificityi

Detected in neonate brain synaptosomes (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000002771.
ExpressionAtlasiQ03391. baseline and differential.
GenevisibleiQ03391. MM.

Interactioni

Subunit structurei

Heterotetramer. Forms heterotetrameric channels composed of two zeta subunits (GRIN1), and two epsilon subunits (GRIN2A, GRIN2B, GRIN2C or GRIN2D) (in vitro) (PubMed:1385220). In vivo, the subunit composition may depend on the expression levels of the different subunits (Probable). Interacts with PDZ domains of PATJ and DLG4 (By similarity).By similarityCurated1 Publication

Protein-protein interaction databases

BioGridi200071. 4 interactors.
IntActiQ03391. 2 interactors.
MINTiMINT-4103932.
STRINGi10090.ENSMUSP00000002848.

Structurei

3D structure databases

ProteinModelPortaliQ03391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni536 – 538Glutamate bindingBy similarity3
Regioni628 – 647Pore-formingBy similarityAdd BLAST20
Regioni714 – 715Glutamate bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1321 – 1323PDZ-bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi278 – 283Poly-Gly6
Compositional biasi905 – 913Poly-Pro9
Compositional biasi1030 – 1035Poly-Ala6
Compositional biasi1197 – 1201Poly-Pro5

Domaini

A hydrophobic region that gives rise to the prediction of a transmembrane span does not cross the membrane, but is part of a discontinuously helical region that dips into the membrane and is probably part of the pore and of the selectivity filter.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1053. Eukaryota.
ENOG410XNUR. LUCA.
GeneTreeiENSGT00760000119186.
HOGENOMiHOG000113803.
HOVERGENiHBG052637.
InParanoidiQ03391.
KOiK05212.
OMAiWDYLPPR.
OrthoDBiEOG091G09KH.
TreeFamiTF314731.

Family and domain databases

InterProiView protein in InterPro
IPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
PfamiView protein in Pfam
PF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
PRINTSiPR00177. NMDARECEPTOR.
SMARTiView protein in SMART
SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGAGGPRGP RGPAKMLLLL ALACASPFPE EVPGPGAAGG GTGGARPLNV
60 70 80 90 100
ALVFSGPAYA AEAARLGPAV AAAVRSPGLD VRPVALVLNG SDPRSLVLQL
110 120 130 140 150
CDLLSGLRVH GVVFEDDSRA PAVAPILDFL SAQTSLPIVA VHGGAALVLT
160 170 180 190 200
PKEKGSTFLQ LGSSTEQQLQ VIFEVLEEYD WTSFVAVTTR APGHRAFLSY
210 220 230 240 250
IEVLTDGSLV GWEHRGALTL DPGAGEAVLG AQLRSVSAQI RLLFCAREEA
260 270 280 290 300
EPVFRAAEEA GLTGPGYVWF MVGPQLAGGG GSGVPGEPLL LPGGAPLPAG
310 320 330 340 350
LFAVRSAGWR DDLARRVAAG VAVVARGAQA LLRDYGFLPE LGHDCRAQNR
360 370 380 390 400
THRGESLHRY FMNITWDNRD YSFNEDGFLV NPSLVVISLT RDRTWEVVGS
410 420 430 440 450
WEQQTLRLKY PLWSRYGRFL QPVDDTQHLT VATLEERPFV IVEPADPISG
460 470 480 490 500
TCIRDSVPCR SQLNRTHSPP PDAPRPEKRC CKGFCIDILK RLAHTIGFSY
510 520 530 540 550
DLYLVTNGKH GKKIDGVWNG MIGEVFYQRA DMAIGSLTIN EERSEIVDFS
560 570 580 590 600
VPFVETGISV MVARSNGTVS PSAFLEPYSP AVWVMMFVMC LTVVAVTVFI
610 620 630 640 650
FEYLSPVGYN RSLATGKRPG GSTFTIGKSI WLLWALVFNN SVPVENPRGT
660 670 680 690 700
TSKIMVLVWA FFAVIFLASY TANLAAFMIQ EEYVDTVSGL SDRKFQRPQE
710 720 730 740 750
QYPPLKFGTV PNGSTEKNIR SNYPDMHSYM VRYNQPRVEE ALTQLKAGKL
760 770 780 790 800
DAFIYDAAVL NYMARKDEGC KLVTIGSGKV FATTGYGIAL HKGSRWKRPI
810 820 830 840 850
DLALLQFLGD DEIEMLERLW LSGICHNDKI EVMSSKLDID NMAGVFYMLL
860 870 880 890 900
VAMGLSLLVF AWEHLVYWRL RHCLGPTHRM DFLLAFSRGM YSCCSAEAAP
910 920 930 940 950
PPAKPPPPPQ PLPSPAYPAA RPPPGPAPFV PRERAAADRW RRAKGTGPPG
960 970 980 990 1000
GAALADGFHR YYGPIEPQGL GLGEARAAPR GAAGRPLSPP TTQPPQKPPP
1010 1020 1030 1040 1050
SYFAIVREQE PAEPPAGAFP GFPSPPAPPA AAAAAVGPPL CRLAFEDESP
1060 1070 1080 1090 1100
PAPSRWPRSD PESQPLLGGG AGGPSAGAPT APPPRRAAPP PCAYLDLEPS
1110 1120 1130 1140 1150
PSDSEDSESL GGASLGGLEP WWFADFPYPY AERLGPPPGR YWSVDKLGGW
1160 1170 1180 1190 1200
RAGSWDYLPP RGGPAWHCRH CASLELLPPP RHLSCSHDGL DGGWWAPPPP
1210 1220 1230 1240 1250
PWAAGPPPRR RARCGCPRPH PHRPRASHRA PAAAPHHHRH RRAAGGWDLP
1260 1270 1280 1290 1300
PPAPTSRSLE DLSSCPRAAP TRRLTGPSRH ARRCPHAAHW GPPLPTASHR
1310 1320
RHRGGDLGTR RGSAHFSSLE SEV
Length:1,323
Mass (Da):142,963
Last modified:July 27, 2011 - v3
Checksum:i3DF8860D19677586
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1087A → T in BAA02254 (PubMed:1385220).Curated1
Sequence conflicti1208 – 1209PR → AP in BAA02254 (PubMed:1385220).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB154245 mRNA. Translation: BAI44630.1.
D12822 mRNA. Translation: BAA02254.1.
CCDSiCCDS21267.1.
PIRiS27224.
RefSeqiNP_032198.2. NM_008172.2.
XP_011249107.1. XM_011250805.2.
UniGeneiMm.322594.

Genome annotation databases

EnsembliENSMUST00000002848; ENSMUSP00000002848; ENSMUSG00000002771.
ENSMUST00000211713; ENSMUSP00000147663; ENSMUSG00000002771.
GeneIDi14814.
KEGGimmu:14814.
UCSCiuc009gxm.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiNMDE4_MOUSE
AccessioniPrimary (citable) accession number: Q03391
Secondary accession number(s): C9K0Z5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: November 22, 2017
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families