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Protein

Glutamate receptor ionotropic, NMDA 2D

Gene

Grin2d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei639 – 6391Functional determinant of NMDA receptorsBy similarity

GO - Molecular functioni

GO - Biological processi

  • adult locomotory behavior Source: MGI
  • ionotropic glutamate receptor signaling pathway Source: GOC
  • ion transmembrane transport Source: GOC
  • regulation of sensory perception of pain Source: MGI
  • startle response Source: MGI
  • synaptic transmission, glutamatergic Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium

Enzyme and pathway databases

ReactomeiREACT_309838. CREB phosphorylation through the activation of CaMKII.
REACT_331187. Unblocking of NMDA receptor, glutamate binding and activation.
REACT_331823. Ras activation uopn Ca2+ infux through NMDA receptor.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 2D
Short name:
GluN2D
Alternative name(s):
Glutamate [NMDA] receptor subunit epsilon-4
N-methyl D-aspartate receptor subtype 2D
Short name:
NMDAR2D
Short name:
NR2D
Gene namesi
Name:Grin2d
Synonyms:GluN2D
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:95823. Grin2d.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 580553ExtracellularSequence AnalysisAdd
BLAST
Transmembranei581 – 60121HelicalSequence AnalysisAdd
BLAST
Topological domaini602 – 65352CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei654 – 67421HelicalSequence AnalysisAdd
BLAST
Topological domaini675 – 841167ExtracellularSequence AnalysisAdd
BLAST
Transmembranei842 – 86221HelicalSequence AnalysisAdd
BLAST
Topological domaini863 – 1323461CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 13231296Glutamate receptor ionotropic, NMDA 2DPRO_0000011584Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi363 – 3631N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi452 ↔ 480By similarity
Disulfide bondi459 ↔ 481By similarity
Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi566 – 5661N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi770 ↔ 825By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ03391.
PRIDEiQ03391.

PTM databases

PhosphoSiteiQ03391.

Expressioni

Gene expression databases

BgeeiQ03391.
ExpressionAtlasiQ03391. baseline.
GenevisibleiQ03391. MM.

Interactioni

Subunit structurei

Interacts with PDZ domains of INADL and DLG4 (By similarity). Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B).By similarity

Protein-protein interaction databases

BioGridi200071. 2 interactions.
IntActiQ03391. 2 interactions.
MINTiMINT-4103932.
STRINGi10090.ENSMUSP00000002848.

Structurei

3D structure databases

ProteinModelPortaliQ03391.
SMRiQ03391. Positions 426-564, 686-826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1321 – 13233PDZ-bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi278 – 2836Poly-Gly
Compositional biasi905 – 9139Poly-Pro
Compositional biasi1030 – 10356Poly-Ala
Compositional biasi1197 – 12015Poly-Pro

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG282132.
GeneTreeiENSGT00760000119186.
HOGENOMiHOG000113803.
HOVERGENiHBG052637.
InParanoidiQ03391.
KOiK05212.
OMAiWDYLPPR.
OrthoDBiEOG72ZCD1.
TreeFamiTF314731.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGAGGPRGP RGPAKMLLLL ALACASPFPE EVPGPGAAGG GTGGARPLNV
60 70 80 90 100
ALVFSGPAYA AEAARLGPAV AAAVRSPGLD VRPVALVLNG SDPRSLVLQL
110 120 130 140 150
CDLLSGLRVH GVVFEDDSRA PAVAPILDFL SAQTSLPIVA VHGGAALVLT
160 170 180 190 200
PKEKGSTFLQ LGSSTEQQLQ VIFEVLEEYD WTSFVAVTTR APGHRAFLSY
210 220 230 240 250
IEVLTDGSLV GWEHRGALTL DPGAGEAVLG AQLRSVSAQI RLLFCAREEA
260 270 280 290 300
EPVFRAAEEA GLTGPGYVWF MVGPQLAGGG GSGVPGEPLL LPGGAPLPAG
310 320 330 340 350
LFAVRSAGWR DDLARRVAAG VAVVARGAQA LLRDYGFLPE LGHDCRAQNR
360 370 380 390 400
THRGESLHRY FMNITWDNRD YSFNEDGFLV NPSLVVISLT RDRTWEVVGS
410 420 430 440 450
WEQQTLRLKY PLWSRYGRFL QPVDDTQHLT VATLEERPFV IVEPADPISG
460 470 480 490 500
TCIRDSVPCR SQLNRTHSPP PDAPRPEKRC CKGFCIDILK RLAHTIGFSY
510 520 530 540 550
DLYLVTNGKH GKKIDGVWNG MIGEVFYQRA DMAIGSLTIN EERSEIVDFS
560 570 580 590 600
VPFVETGISV MVARSNGTVS PSAFLEPYSP AVWVMMFVMC LTVVAVTVFI
610 620 630 640 650
FEYLSPVGYN RSLATGKRPG GSTFTIGKSI WLLWALVFNN SVPVENPRGT
660 670 680 690 700
TSKIMVLVWA FFAVIFLASY TANLAAFMIQ EEYVDTVSGL SDRKFQRPQE
710 720 730 740 750
QYPPLKFGTV PNGSTEKNIR SNYPDMHSYM VRYNQPRVEE ALTQLKAGKL
760 770 780 790 800
DAFIYDAAVL NYMARKDEGC KLVTIGSGKV FATTGYGIAL HKGSRWKRPI
810 820 830 840 850
DLALLQFLGD DEIEMLERLW LSGICHNDKI EVMSSKLDID NMAGVFYMLL
860 870 880 890 900
VAMGLSLLVF AWEHLVYWRL RHCLGPTHRM DFLLAFSRGM YSCCSAEAAP
910 920 930 940 950
PPAKPPPPPQ PLPSPAYPAA RPPPGPAPFV PRERAAADRW RRAKGTGPPG
960 970 980 990 1000
GAALADGFHR YYGPIEPQGL GLGEARAAPR GAAGRPLSPP TTQPPQKPPP
1010 1020 1030 1040 1050
SYFAIVREQE PAEPPAGAFP GFPSPPAPPA AAAAAVGPPL CRLAFEDESP
1060 1070 1080 1090 1100
PAPSRWPRSD PESQPLLGGG AGGPSAGAPT APPPRRAAPP PCAYLDLEPS
1110 1120 1130 1140 1150
PSDSEDSESL GGASLGGLEP WWFADFPYPY AERLGPPPGR YWSVDKLGGW
1160 1170 1180 1190 1200
RAGSWDYLPP RGGPAWHCRH CASLELLPPP RHLSCSHDGL DGGWWAPPPP
1210 1220 1230 1240 1250
PWAAGPPPRR RARCGCPRPH PHRPRASHRA PAAAPHHHRH RRAAGGWDLP
1260 1270 1280 1290 1300
PPAPTSRSLE DLSSCPRAAP TRRLTGPSRH ARRCPHAAHW GPPLPTASHR
1310 1320
RHRGGDLGTR RGSAHFSSLE SEV
Length:1,323
Mass (Da):142,963
Last modified:July 27, 2011 - v3
Checksum:i3DF8860D19677586
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1087 – 10871A → T in BAA02254 (PubMed:1385220).Curated
Sequence conflicti1208 – 12092PR → AP in BAA02254 (PubMed:1385220).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB154245 mRNA. Translation: BAI44630.1.
D12822 mRNA. Translation: BAA02254.1.
CCDSiCCDS21267.1.
PIRiS27224.
RefSeqiNP_032198.2. NM_008172.2.
UniGeneiMm.322594.

Genome annotation databases

EnsembliENSMUST00000002848; ENSMUSP00000002848; ENSMUSG00000002771.
GeneIDi14814.
KEGGimmu:14814.
UCSCiuc009gxm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB154245 mRNA. Translation: BAI44630.1.
D12822 mRNA. Translation: BAA02254.1.
CCDSiCCDS21267.1.
PIRiS27224.
RefSeqiNP_032198.2. NM_008172.2.
UniGeneiMm.322594.

3D structure databases

ProteinModelPortaliQ03391.
SMRiQ03391. Positions 426-564, 686-826.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200071. 2 interactions.
IntActiQ03391. 2 interactions.
MINTiMINT-4103932.
STRINGi10090.ENSMUSP00000002848.

Chemistry

GuidetoPHARMACOLOGYi459.

PTM databases

PhosphoSiteiQ03391.

Proteomic databases

PaxDbiQ03391.
PRIDEiQ03391.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002848; ENSMUSP00000002848; ENSMUSG00000002771.
GeneIDi14814.
KEGGimmu:14814.
UCSCiuc009gxm.1. mouse.

Organism-specific databases

CTDi2906.
MGIiMGI:95823. Grin2d.

Phylogenomic databases

eggNOGiNOG282132.
GeneTreeiENSGT00760000119186.
HOGENOMiHOG000113803.
HOVERGENiHBG052637.
InParanoidiQ03391.
KOiK05212.
OMAiWDYLPPR.
OrthoDBiEOG72ZCD1.
TreeFamiTF314731.

Enzyme and pathway databases

ReactomeiREACT_309838. CREB phosphorylation through the activation of CaMKII.
REACT_331187. Unblocking of NMDA receptor, glutamate binding and activation.
REACT_331823. Ras activation uopn Ca2+ infux through NMDA receptor.

Miscellaneous databases

NextBioi287011.
PROiQ03391.
SOURCEiSearch...

Gene expression databases

BgeeiQ03391.
ExpressionAtlasiQ03391. baseline.
GenevisibleiQ03391. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Expression of mRNA in mouse spinal cord."
    Nishizwa M., Ito S.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
    Tissue: Spinal cord.
  2. "Cloning and expression of the epsilon 4 subunit of the NMDA receptor channel."
    Ikeda K., Nagasawa M., Mori H., Araki K., Sakimura K., Watanabe M., Inoue Y., Mishina M.
    FEBS Lett. 313:34-38(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. Ikeda K., Nagasawa M., Mori H., Araki K., Sakimura K., Watanabe M., Inoue Y., Mishina M.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiNMDE4_MOUSE
AccessioniPrimary (citable) accession number: Q03391
Secondary accession number(s): C9K0Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.