Vacuolar protein sorting-associated protein 72 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

Q03388 (VPS72_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 72

Alternative name(s):
SWR complex protein 2

Gene names

Name:	VPS72
Synonyms:	SWC2
Ordered Locus Names:	YDR485C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the catalytic exchange of histone H2A for the H2A variant HTZ1, an euchromatin-specific factor, leading to chromatin remodeling and changes in transcription of targeted genes. Indirectly involved in vacuolar protein sorting. Ref.5 Ref.6 Ref.9 Ref.10
Subunit structure	Belongs to the SWR1 complex at least composed of ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWR1 and HTZ1. Interacts with HTZ1. Ref.6 Ref.9 Ref.10 Ref.11
Subcellular location	Nucleus Ref.7.
Miscellaneous	Present with 358 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the VPS72/YL1 family.
Sequence caution	The sequence AAB64928.1 differs from that shown. Reason: Erroneous initiation. The sequence AAT93172.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone exchange Inferred from mutant phenotype Ref.11. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	Swr1 complex Inferred from direct assay Ref.9 Ref.6 Ref.11. Source: SGD cytoplasm Inferred from direct assay PubMed 11914276. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 795

795

Vacuolar protein sorting-associated protein 72

PRO_0000065909

Regions

Region

1 – 281

281

Interaction with HTZ1

Coiled coil

28 – 63

Potential

Coiled coil

121 – 150

Potential

Coiled coil

204 – 281

Potential

Compositional bias

57 – 85

Asp-rich

Compositional bias

315 – 343

Lys-rich

Amino acid modifications

Modified residue

396

Phosphoserine Ref.12 Ref.13

Modified residue

425

Phosphoserine Ref.13

Modified residue

471

Phosphoserine Ref.13

Modified residue

472

Phosphoserine Ref.13

Modified residue

502

Phosphoserine Ref.13

Modified residue

538

Phosphoserine Ref.13

Sequences

Sequence

Length

Mass (Da)

Tools

Q03388 [UniParc].

Last modified April 26, 2005. Version 2.
Checksum: 6D3053EBB610DB95
Show »

FASTA

795

90,580

        10         20         30         40         50         60 
MSDEGADKSL DTNTEFIIQT RSRRSNAGNK LQKLLEQELR DIESTKRQIS SYKNGNDDEE 

        70         80         90        100        110        120 
DEIGLLFQED EDDEDFEMMA KDDDDEGEEK EDETQSIRKE PSQASSEQAA DDLMFSSSES 

       130        140        150        160        170        180 
EDSSNENDED AEEKEIRRQE LLSRKKRNKR LQKGPVVIKK QKPKPKSGEA IPRSHHTHEQ 

       190        200        210        220        230        240 
LNAETLLLNT RRTSKRSSVM ENTMKVYEKL SKAEKKRKII QERIRKHKEQ ESQHMLTQEE 

       250        260        270        280        290        300 
RLRIAKETEK LNILSLDKFK EQEVWKKENR LALQKRQKQK FQPNETILQF LSTAWLMTPA 

       310        320        330        340        350        360 
MELEDRKYWQ EQLNKRDKKK KKYPRKPKKN LNLGKQDASD DKKRESEESI KNDGDVNSLG 

       370        380        390        400        410        420 
ENSSSVHNQK RIEETSTNDT VEGESSPDAA VSRVNSDELK PTALPDVTLD AIANKQSTVD 

       430        440        450        460        470        480 
EAPNSQPQKN IITNEQKITN VGEPIQNLHN EEIKDEMVSA LESRENTFEN SSPAAQVVSQ 

       490        500        510        520        530        540 
RDNSATPTPS NSTGTEDTIL ISPDTDIKGE PEPCLKTEGI ENLSHNVPQE TKSNTDVSFL 

       550        560        570        580        590        600 
KQVTFTDHPQ VAIIDTEESP SKKDTANVDE SSAENSLSTQ TYEGPEQLTS RNFVTLYDFP 

       610        620        630        640        650        660 
NAPPNLKDFN TNLFGDRWSY TNGLSATQRP QDMKTVFHSI LPSPPQSSVP SPTVDISLDL 

       670        680        690        700        710        720 
SALANFPSFG EYDKKIVHQI NTEINKDLEI KIKTQPPTGV FLANGIRKKC LITNKECQYF 

       730        740        750        760        770        780 
DPRTGVPYSD VEAYKIIQRI QDPISKEEGR SDIKRDETTN EDSDDQVRFK WFGFKNGGIY 

       790 
LDLSQRPAKG VPEGF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]	"Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae." Bonangelino C.J., Chavez E.M., Bonifacino J.S. Mol. Biol. Cell 13:2486-2501(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1." Krogan N.J., Keogh M.-C., Datta N., Sawa C., Ryan O.W., Ding H., Haw R.A., Pootoolal J., Tong A., Canadien V., Richards D.P., Wu X., Emili A., Hughes T.R., Buratowski S., Greenblatt J.F. Mol. Cell 12:1565-1576(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ACT1; ARP4; RVB1; RVB2; ARP6; YAF9; VPS71; SWC3; SWC4; SWC5; SWR1 AND HTZ1.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex." Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C. Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ACT1; ARP4; RVB1; RVB2; ARP6; YAF9; VPS71; SWC3; SWC4; SWC5; SWR1 AND HTZ1.
[10]	"A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin." Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J. PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ACT1; ARP4; RVB1; RVB2; ARP6; YAF9; VPS71; SWC3; SWC4; SWC5; SWR1 AND HTZ1.
[11]	"Swc2 is a widely conserved H2AZ-binding module essential for ATP-dependent histone exchange." Wu W.-H., Alami S., Luk E., Wu C.-H., Sen S., Mizuguchi G., Wei D., Wu C. Nat. Struct. Mol. Biol. 12:1064-1071(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HTZ1.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-396, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-396; SER-425; SER-471; SER-472; SER-502 AND SER-538, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33050 Genomic DNA. Translation: AAB64928.1. Different initiation. AY693153 Genomic DNA. Translation: AAT93172.1. Different initiation. BK006938 Genomic DNA. Translation: DAA12318.1.
PIR	S69652.
RefSeq	NP_010773.4. NM_001180793.3.
3D structure databases
ProteinModelPortal	Q03388.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1008N.
IntAct	Q03388. 14 interactions.
MINT	MINT-2782183.
STRING	4932.YDR485C.
Proteomic databases
PaxDb	Q03388.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR485C; YDR485C; YDR485C.
GeneID	852096.
KEGG	sce:YDR485C.
Organism-specific databases
CYGD	YDR485c.
SGD	S000002893. VPS72.
Phylogenomic databases
eggNOG	NOG253988.
HOGENOM	HOG000141998.
KO	K11664.
OMA	LLFQEDE.
OrthoDB	EOG42NN8G.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30010-MONOMER.
Gene expression databases
Genevestigator	Q03388.
GermOnline	YDR485C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008895. YL1. IPR013272. YL1_C. [Graphical view]
Pfam	PF05764. YL1. 1 hit. PF08265. YL1_C. 1 hit. [Graphical view]
SMART	SM00993. YL1_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970433.

Entry information

Entry name

VPS72_YEAST

Accession

Primary (citable) accession number: Q03388
Secondary accession number(s): D6VTA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	May 29, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents