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Q03388

- VPS72_YEAST

UniProt

Q03388 - VPS72_YEAST

Protein

Vacuolar protein sorting-associated protein 72

Gene

VPS72

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Participates in the catalytic exchange of histone H2A for the H2A variant HTZ1, an euchromatin-specific factor, leading to chromatin remodeling and changes in transcription of targeted genes. Indirectly involved in vacuolar protein sorting.4 Publications

    GO - Molecular functioni

    1. histone binding Source: SGD

    GO - Biological processi

    1. chromatin remodeling Source: SGD
    2. histone exchange Source: SGD
    3. regulation of transcription, DNA-templated Source: InterPro

    Keywords - Molecular functioni

    Chromatin regulator

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30010-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 72
    Alternative name(s):
    SWR complex protein 2
    Gene namesi
    Name:VPS72
    Synonyms:SWC2
    Ordered Locus Names:YDR485C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR485c.
    SGDiS000002893. VPS72.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. Swr1 complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 795795Vacuolar protein sorting-associated protein 72PRO_0000065909Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei425 – 4251Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03388.
    PaxDbiQ03388.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03388.

    Interactioni

    Subunit structurei

    Belongs to the SWR1 complex at least composed of ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWR1 and HTZ1. Interacts with HTZ1.4 Publications

    Protein-protein interaction databases

    BioGridi32537. 350 interactions.
    DIPiDIP-1008N.
    IntActiQ03388. 14 interactions.
    MINTiMINT-2782183.
    STRINGi4932.YDR485C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03388.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 281281Interaction with HTZ1Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili28 – 6336Sequence AnalysisAdd
    BLAST
    Coiled coili121 – 15030Sequence AnalysisAdd
    BLAST
    Coiled coili204 – 28178Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi57 – 8529Asp-richAdd
    BLAST
    Compositional biasi315 – 34329Lys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the VPS72/YL1 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG253988.
    HOGENOMiHOG000141998.
    KOiK11664.
    OMAiLLFQEDE.
    OrthoDBiEOG78PVK3.

    Family and domain databases

    InterProiIPR008895. YL1.
    IPR013272. YL1_C.
    [Graphical view]
    PfamiPF05764. YL1. 1 hit.
    PF08265. YL1_C. 1 hit.
    [Graphical view]
    SMARTiSM00993. YL1_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03388-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDEGADKSL DTNTEFIIQT RSRRSNAGNK LQKLLEQELR DIESTKRQIS    50
    SYKNGNDDEE DEIGLLFQED EDDEDFEMMA KDDDDEGEEK EDETQSIRKE 100
    PSQASSEQAA DDLMFSSSES EDSSNENDED AEEKEIRRQE LLSRKKRNKR 150
    LQKGPVVIKK QKPKPKSGEA IPRSHHTHEQ LNAETLLLNT RRTSKRSSVM 200
    ENTMKVYEKL SKAEKKRKII QERIRKHKEQ ESQHMLTQEE RLRIAKETEK 250
    LNILSLDKFK EQEVWKKENR LALQKRQKQK FQPNETILQF LSTAWLMTPA 300
    MELEDRKYWQ EQLNKRDKKK KKYPRKPKKN LNLGKQDASD DKKRESEESI 350
    KNDGDVNSLG ENSSSVHNQK RIEETSTNDT VEGESSPDAA VSRVNSDELK 400
    PTALPDVTLD AIANKQSTVD EAPNSQPQKN IITNEQKITN VGEPIQNLHN 450
    EEIKDEMVSA LESRENTFEN SSPAAQVVSQ RDNSATPTPS NSTGTEDTIL 500
    ISPDTDIKGE PEPCLKTEGI ENLSHNVPQE TKSNTDVSFL KQVTFTDHPQ 550
    VAIIDTEESP SKKDTANVDE SSAENSLSTQ TYEGPEQLTS RNFVTLYDFP 600
    NAPPNLKDFN TNLFGDRWSY TNGLSATQRP QDMKTVFHSI LPSPPQSSVP 650
    SPTVDISLDL SALANFPSFG EYDKKIVHQI NTEINKDLEI KIKTQPPTGV 700
    FLANGIRKKC LITNKECQYF DPRTGVPYSD VEAYKIIQRI QDPISKEEGR 750
    SDIKRDETTN EDSDDQVRFK WFGFKNGGIY LDLSQRPAKG VPEGF 795
    Length:795
    Mass (Da):90,580
    Last modified:April 26, 2005 - v2
    Checksum:i6D3053EBB610DB95
    GO

    Sequence cautioni

    The sequence AAB64928.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAT93172.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33050 Genomic DNA. Translation: AAB64928.1. Different initiation.
    AY693153 Genomic DNA. Translation: AAT93172.1. Different initiation.
    BK006938 Genomic DNA. Translation: DAA12318.1.
    PIRiS69652.
    RefSeqiNP_010773.4. NM_001180793.3.

    Genome annotation databases

    EnsemblFungiiYDR485C; YDR485C; YDR485C.
    GeneIDi852096.
    KEGGisce:YDR485C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33050 Genomic DNA. Translation: AAB64928.1 . Different initiation.
    AY693153 Genomic DNA. Translation: AAT93172.1 . Different initiation.
    BK006938 Genomic DNA. Translation: DAA12318.1 .
    PIRi S69652.
    RefSeqi NP_010773.4. NM_001180793.3.

    3D structure databases

    ProteinModelPortali Q03388.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32537. 350 interactions.
    DIPi DIP-1008N.
    IntActi Q03388. 14 interactions.
    MINTi MINT-2782183.
    STRINGi 4932.YDR485C.

    Proteomic databases

    MaxQBi Q03388.
    PaxDbi Q03388.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR485C ; YDR485C ; YDR485C .
    GeneIDi 852096.
    KEGGi sce:YDR485C.

    Organism-specific databases

    CYGDi YDR485c.
    SGDi S000002893. VPS72.

    Phylogenomic databases

    eggNOGi NOG253988.
    HOGENOMi HOG000141998.
    KOi K11664.
    OMAi LLFQEDE.
    OrthoDBi EOG78PVK3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30010-MONOMER.

    Miscellaneous databases

    NextBioi 970433.
    PROi Q03388.

    Gene expression databases

    Genevestigatori Q03388.

    Family and domain databases

    InterProi IPR008895. YL1.
    IPR013272. YL1_C.
    [Graphical view ]
    Pfami PF05764. YL1. 1 hit.
    PF08265. YL1_C. 1 hit.
    [Graphical view ]
    SMARTi SM00993. YL1_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    5. "Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae."
      Bonangelino C.J., Chavez E.M., Bonifacino J.S.
      Mol. Biol. Cell 13:2486-2501(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: FUNCTION, INTERACTION WITH ACT1; ARP4; RVB1; RVB2; ARP6; YAF9; VPS71; SWC3; SWC4; SWC5; SWR1 AND HTZ1.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex."
      Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C.
      Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ACT1; ARP4; RVB1; RVB2; ARP6; YAF9; VPS71; SWC3; SWC4; SWC5; SWR1 AND HTZ1.
    10. "A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin."
      Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J.
      PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ACT1; ARP4; RVB1; RVB2; ARP6; YAF9; VPS71; SWC3; SWC4; SWC5; SWR1 AND HTZ1.
    11. "Swc2 is a widely conserved H2AZ-binding module essential for ATP-dependent histone exchange."
      Wu W.-H., Alami S., Luk E., Wu C.-H., Sen S., Mizuguchi G., Wei D., Wu C.
      Nat. Struct. Mol. Biol. 12:1064-1071(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HTZ1.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-425, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVPS72_YEAST
    AccessioniPrimary (citable) accession number: Q03388
    Secondary accession number(s): D6VTA8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 358 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3