Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ral guanine nucleotide dissociation stimulator

Gene

Ralgds

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap.

GO - Molecular functioni

  • Ral guanyl-nucleotide exchange factor activity Source: RGD

GO - Biological processi

  • Ras protein signal transduction Source: Reactome
  • small GTPase mediated signal transduction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-RNO-171007. p38MAPK events.
R-RNO-187706. Signalling to p38 via RIT and RIN.

Names & Taxonomyi

Protein namesi
Recommended name:
Ral guanine nucleotide dissociation stimulator
Short name:
RalGDS
Alternative name(s):
Ral guanine nucleotide exchange factor
Short name:
RalGEF
Gene namesi
Name:Ralgds
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3533. Ralgds.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 895895Ral guanine nucleotide dissociation stimulatorPRO_0000068879Add
BLAST

Proteomic databases

PaxDbiQ03386.
PRIDEiQ03386.

PTM databases

iPTMnetiQ03386.
PhosphoSiteiQ03386.

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Interactioni

Subunit structurei

Interacts with RIT1 and RIT2.By similarity

Protein-protein interaction databases

IntActiQ03386. 1 interaction.
STRINGi10116.ENSRNOP00000013809.

Structurei

Secondary structure

1
895
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi779 – 78911Combined sources
Beta strandi791 – 80111Combined sources
Helixi806 – 81611Combined sources
Helixi824 – 8263Combined sources
Beta strandi827 – 83610Combined sources
Beta strandi838 – 8403Combined sources
Helixi847 – 8504Combined sources
Beta strandi858 – 8636Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFDX-ray2.10A/C778-864[»]
1LXDX-ray2.40A/B767-864[»]
ProteinModelPortaliQ03386.
SMRiQ03386. Positions 778-864.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03386.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 237126N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini367 – 629263Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini779 – 86688Ras-associatingPROSITE-ProRule annotationAdd
BLAST

Domaini

The Ras-associating domain interacts with Ras.

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3629. Eukaryota.
ENOG410ZQ4B. LUCA.
HOGENOMiHOG000231592.
HOVERGENiHBG005864.
InParanoidiQ03386.
KOiK08732.
PhylomeDBiQ03386.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR015758. RalGDS.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR23113:SF35. PTHR23113:SF35. 3 hits.
PfamiPF00788. RA. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQRMWAEAS GPIGGAEPLF PGSRRSRSVW DAVRLEVGVP DSCPVVLHSF
60 70 80 90 100
TQLDPDLPRL ESSTQEIGEE LINGVIYSIS LRKVQLYPGA TKGQRWLGCE
110 120 130 140 150
NESALNLYET CKVRTVKAGT LEKLVEHLVP AFQGSDLSYV TVFLCTYRAF
160 170 180 190 200
TTTQQVLDLL FKRYGCILPY SSEDGGPQDQ LKNAISSILG TWLDQYSEDF
210 220 230 240 250
CQPPDFPCLK QLVAYVQLNM PGSDLERRAH LLLAQLEDLE PSEVEPEALS
260 270 280 290 300
PAPVLSLKPA SQLEPAPALL LTPSRAVAST PVREPAPVPV LASSPVVAPA
310 320 330 340 350
SELEPALEPP LDPEPTLAPA PELDPTVSQS LHLEPAPVPA PALEPSWPLP
360 370 380 390 400
ETTENGLCAK PHLLLFPPDL VAEQFTLMDA ELFKKVVPYH CLGSIWSQRA
410 420 430 440 450
KKGKEHLAPT IRATVAQFNN VANCVITTCL GDQSMKASDR ARVVEHWIEV
460 470 480 490 500
ARECRVLKNF SSLYAILSAL QSNAIHRLKK TWEEVSRGSF RVFQKLSEIF
510 520 530 540 550
SDENNYSLSR ELLIKEGTSK FATLEMNPRR TQRRQKETGV IQGTVPYLGT
560 570 580 590 600
FLTDLVMLDT AMKDYLYGRL INFEKRRKEF EVIAQIKLLQ SACNNYSIVP
610 620 630 640 650
EEHFGAWFRA MGRLSEAESY NLSCELEPPS ESASNTLRSK KSTAIVKRWE
660 670 680 690 700
RRQAPSTELS TSSSAHSKSC DQLRCSPYLS SGDITDALSV HSAGSSTSDV
710 720 730 740 750
EEINMSFVPE SPDGQEKKFW ESASQSSPET SGISSASSST SSSSASTTPV
760 770 780 790 800
STTRTHKRSV SGVCSYSSSL PLYNQQVGDC CIIRVSLDVD NGNMYKSILV
810 820 830 840 850
TSQDKAPTVI RKAMDKHNLD EDEPEDYELL QIISEDHKLK IPENANVFYA
860 870 880 890
MNSAANYDFI LKKRAFTKGA KVKHGASSTL PRMKQKGLRI ARGIF
Length:895
Mass (Da):98,869
Last modified:June 1, 1994 - v1
Checksum:i43E1674675A4E1C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07925 mRNA. Translation: AAA41259.1.
RefSeqiNP_062123.2. NM_019250.2.
UniGeneiRn.40174.

Genome annotation databases

GeneIDi29622.
KEGGirno:29622.
UCSCiRGD:3533. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07925 mRNA. Translation: AAA41259.1.
RefSeqiNP_062123.2. NM_019250.2.
UniGeneiRn.40174.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFDX-ray2.10A/C778-864[»]
1LXDX-ray2.40A/B767-864[»]
ProteinModelPortaliQ03386.
SMRiQ03386. Positions 778-864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ03386. 1 interaction.
STRINGi10116.ENSRNOP00000013809.

PTM databases

iPTMnetiQ03386.
PhosphoSiteiQ03386.

Proteomic databases

PaxDbiQ03386.
PRIDEiQ03386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29622.
KEGGirno:29622.
UCSCiRGD:3533. rat.

Organism-specific databases

CTDi5900.
RGDi3533. Ralgds.

Phylogenomic databases

eggNOGiKOG3629. Eukaryota.
ENOG410ZQ4B. LUCA.
HOGENOMiHOG000231592.
HOVERGENiHBG005864.
InParanoidiQ03386.
KOiK08732.
PhylomeDBiQ03386.

Enzyme and pathway databases

ReactomeiR-RNO-171007. p38MAPK events.
R-RNO-187706. Signalling to p38 via RIT and RIN.

Miscellaneous databases

EvolutionaryTraceiQ03386.
PROiQ03386.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR015758. RalGDS.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR23113:SF35. PTHR23113:SF35. 3 hits.
PfamiPF00788. RA. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of a guanine nucleotide dissociation stimulator for a ras-related GTPase."
    Albright C.F., Giddings B.W., Liu J., Vito M., Weinberg R.A.
    EMBO J. 12:339-347(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fibroblast.
  2. "Three-dimensional structure of the Ras-interacting domain of RalGDS."
    Huang L., Weng X., Hofer F., Martin G.S., Kim S.H.
    Nat. Struct. Biol. 4:609-615(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 778-864.

Entry informationi

Entry nameiGNDS_RAT
AccessioniPrimary (citable) accession number: Q03386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.