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Protein

Ral guanine nucleotide dissociation stimulator

Gene

Ralgds

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-171007. p38MAPK events.

Names & Taxonomyi

Protein namesi
Recommended name:
Ral guanine nucleotide dissociation stimulator
Short name:
RalGDS
Alternative name(s):
Ral guanine nucleotide exchange factor
Short name:
RalGEF
Gene namesi
Name:Ralgds
Synonyms:Rgds
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107485. Ralgds.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 852852Ral guanine nucleotide dissociation stimulatorPRO_0000068878Add
BLAST

Proteomic databases

MaxQBiQ03385.
PaxDbiQ03385.
PRIDEiQ03385.

PTM databases

iPTMnetiQ03385.

Expressioni

Gene expression databases

BgeeiQ03385.
CleanExiMM_RALGDS.
ExpressionAtlasiQ03385. baseline and differential.
GenevisibleiQ03385. MM.

Interactioni

Subunit structurei

Interacts with RIT1 and RIT2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
oogenesinQ810D95EBI-923705,EBI-923698

Protein-protein interaction databases

BioGridi202878. 1 interaction.
IntActiQ03385. 2 interactions.
MINTiMINT-1511030.
STRINGi10090.ENSMUSP00000097812.

Structurei

3D structure databases

ProteinModelPortaliQ03385.
SMRiQ03385. Positions 59-268, 291-586, 735-821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 194138N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini324 – 586263Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini736 – 82388Ras-associatingPROSITE-ProRule annotationAdd
BLAST

Domaini

The Ras-associating domain interacts with Ras.By similarity

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3629. Eukaryota.
ENOG410ZUT5. LUCA.
GeneTreeiENSGT00810000125349.
HOGENOMiHOG000231592.
HOVERGENiHBG005864.
InParanoidiQ03385.
KOiK08732.
OrthoDBiEOG76DTRS.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR015758. RalGDS.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR23113:SF35. PTHR23113:SF35. 4 hits.
PfamiPF00788. RA. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVDCQSSTQ EIGEELINGV IYSISLRKVQ LHQGATKGQR WLGCENESAL
60 70 80 90 100
NLYETCKVRT VKAGTLEKLV EHLVPAFQGS DLSYVTVFLC TYRAFTTTQQ
110 120 130 140 150
VLDLLFKRYG RCDALTASSR YGCILPYSSE DGGPQDQLKN AISSILGTWL
160 170 180 190 200
DQYSEDFCQP PDFPCLKQLV AYVQLNMPGS DLERRAHLLL AQLEDLEPSE
210 220 230 240 250
AESEALSPAP VLSLKPASQL EPALLLTPSQ VVTSTPVREP AAAPVPVLAS
260 270 280 290 300
SPVVAPAPEL EPVPEPPQEP EPSLALAPEL EPAVSQSLEL ESAPVPTPAL
310 320 330 340 350
EPSWSLPEAT ENGLTEKPHL LLFPPDLVAE QFTLMDAELF KKVVPYHCLG
360 370 380 390 400
SIWSQRDKKG KEHLAPTIRA TVAQFNNVAN CVITTCLGDQ SMKAPDRARV
410 420 430 440 450
VEHWIEVARE CRALKNFSSL YAILSALQSN AIHRLKKTWE EVSRDSFRVF
460 470 480 490 500
QKLSEIFSDE NNYSLSRELL IKEGTSKFAT LEMNPRRAQR RQKETGVIQG
510 520 530 540 550
TVPYLGTFLT DLVMLDTAMK DYLYGRLINF EKRRKEFEVI AQIKLLQSAC
560 570 580 590 600
NNYSIAPEEH FGTWFRAMER LSEAESYTLS CELEPPSESA SNTLRSKKST
610 620 630 640 650
AIVKRWSDRQ APSTELSTSS SAHSKSCDQL RCSPYLGSGD ITDALSVHSA
660 670 680 690 700
GSSSSDVEEI NMSFVPESPD GQEKKFWESA SQSSPETSGI SSASSSTSSS
710 720 730 740 750
SASTTPVSTT RTHKRSVSGV CSYSSSLPLY NQQVGDCCII RVSLDVDNGN
760 770 780 790 800
MYKSILVTSQ DKAPTVIRKA MDKHNLDEDE PEDYELVQII SEDHKLKIPE
810 820 830 840 850
NANVFYAMNS TANYDFILKK RTFTKGAKVK HGASSTLPRM KQKGLRIAKG

IF
Length:852
Mass (Da):94,202
Last modified:July 27, 2011 - v2
Checksum:i5C6731F7BF3B07B8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti357 – 3571D → A in AAA37714 (PubMed:8094051).Curated
Sequence conflicti691 – 6911S → T in AAA37714 (PubMed:8094051).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07924 mRNA. Translation: AAA37714.1.
AL772249 Genomic DNA. Translation: CAM24090.1.
CH466542 Genomic DNA. Translation: EDL08385.1.
BC027225 mRNA. Translation: AAH27225.1.
CCDSiCCDS15840.1.
PIRiS28415.
RefSeqiNP_033084.2. NM_009058.2.
UniGeneiMm.5236.

Genome annotation databases

EnsembliENSMUST00000028170; ENSMUSP00000028170; ENSMUSG00000026821.
GeneIDi19730.
KEGGimmu:19730.
UCSCiuc008iym.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07924 mRNA. Translation: AAA37714.1.
AL772249 Genomic DNA. Translation: CAM24090.1.
CH466542 Genomic DNA. Translation: EDL08385.1.
BC027225 mRNA. Translation: AAH27225.1.
CCDSiCCDS15840.1.
PIRiS28415.
RefSeqiNP_033084.2. NM_009058.2.
UniGeneiMm.5236.

3D structure databases

ProteinModelPortaliQ03385.
SMRiQ03385. Positions 59-268, 291-586, 735-821.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202878. 1 interaction.
IntActiQ03385. 2 interactions.
MINTiMINT-1511030.
STRINGi10090.ENSMUSP00000097812.

PTM databases

iPTMnetiQ03385.

Proteomic databases

MaxQBiQ03385.
PaxDbiQ03385.
PRIDEiQ03385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028170; ENSMUSP00000028170; ENSMUSG00000026821.
GeneIDi19730.
KEGGimmu:19730.
UCSCiuc008iym.2. mouse.

Organism-specific databases

CTDi5900.
MGIiMGI:107485. Ralgds.

Phylogenomic databases

eggNOGiKOG3629. Eukaryota.
ENOG410ZUT5. LUCA.
GeneTreeiENSGT00810000125349.
HOGENOMiHOG000231592.
HOVERGENiHBG005864.
InParanoidiQ03385.
KOiK08732.
OrthoDBiEOG76DTRS.

Enzyme and pathway databases

ReactomeiR-MMU-171007. p38MAPK events.

Miscellaneous databases

ChiTaRSiRalgds. mouse.
PROiQ03385.
SOURCEiSearch...

Gene expression databases

BgeeiQ03385.
CleanExiMM_RALGDS.
ExpressionAtlasiQ03385. baseline and differential.
GenevisibleiQ03385. MM.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR015758. RalGDS.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR23113:SF35. PTHR23113:SF35. 4 hits.
PfamiPF00788. RA. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a guanine nucleotide dissociation stimulator for a ras-related GTPase."
    Albright C.F., Giddings B.W., Liu J., Vito M., Weinberg R.A.
    EMBO J. 12:339-347(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fibroblast.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiGNDS_MOUSE
AccessioniPrimary (citable) accession number: Q03385
Secondary accession number(s): Q8R077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.