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Protein

Pre-mRNA-splicing factor CWC21

Gene

CWC21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. May function at or prior to the first catalytic step of splicing at the catalytic center of the spliceosome, together with ISY1. May do so by stabilizing the catalytic center or the position of the RNA substrate.2 Publications

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30007-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC21
Alternative name(s):
Complexed with CEF1 protein 21
Gene namesi
Name:CWC21
Ordered Locus Names:YDR482C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR482c.
SGDiS000002890. CWC21.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. U2-type spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 135135Pre-mRNA-splicing factor CWC21PRO_0000123505Add
BLAST

Proteomic databases

PaxDbiQ03375.

Expressioni

Gene expression databases

GenevestigatoriQ03375.

Interactioni

Subunit structurei

Associates with the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts directly with PRP8 (via SCwid domain). Interacts directly with SNU114 (via C-terminus).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TPD3P313831EBI-30409,EBI-1936

Protein-protein interaction databases

BioGridi32534. 76 interactions.
DIPiDIP-1847N.
IntActiQ03375. 6 interactions.
MINTiMINT-407313.
STRINGi4932.YDR482C.

Structurei

3D structure databases

ProteinModelPortaliQ03375.
SMRiQ03375. Positions 67-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 9745Interaction with PRP8Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili65 – 10238Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the CWC21 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000065927.
InParanoidiQ03375.
OMAiKRESTEY.
OrthoDBiEOG7FV43H.

Family and domain databases

InterProiIPR013170. mRNA_splic_Cwf21.
[Graphical view]
PfamiPF08312. cwf21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS
60 70 80 90 100
HDKASRPLAV QKQIETHMEK REIEVQVSEL RDRLEEEETL SEEQIDKKCE
110 120 130
ALRAKLTNEW QEQQRMSSLY TPRKARLTEE QHRHE
Length:135
Mass (Da):15,763
Last modified:November 1, 1996 - v1
Checksum:i9723E1C0A393508B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64929.1.
AY557800 Genomic DNA. Translation: AAS56126.1.
BK006938 Genomic DNA. Translation: DAA12315.1.
PIRiS69649.
RefSeqiNP_010770.3. NM_001180790.3.

Genome annotation databases

EnsemblFungiiYDR482C; YDR482C; YDR482C.
GeneIDi852093.
KEGGisce:YDR482C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64929.1.
AY557800 Genomic DNA. Translation: AAS56126.1.
BK006938 Genomic DNA. Translation: DAA12315.1.
PIRiS69649.
RefSeqiNP_010770.3. NM_001180790.3.

3D structure databases

ProteinModelPortaliQ03375.
SMRiQ03375. Positions 67-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32534. 76 interactions.
DIPiDIP-1847N.
IntActiQ03375. 6 interactions.
MINTiMINT-407313.
STRINGi4932.YDR482C.

Proteomic databases

PaxDbiQ03375.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR482C; YDR482C; YDR482C.
GeneIDi852093.
KEGGisce:YDR482C.

Organism-specific databases

CYGDiYDR482c.
SGDiS000002890. CWC21.

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000065927.
InParanoidiQ03375.
OMAiKRESTEY.
OrthoDBiEOG7FV43H.

Enzyme and pathway databases

BioCyciYEAST:G3O-30007-MONOMER.

Miscellaneous databases

NextBioi970424.

Gene expression databases

GenevestigatoriQ03375.

Family and domain databases

InterProiIPR013170. mRNA_splic_Cwf21.
[Graphical view]
PfamiPF08312. cwf21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome."
    Grainger R.J., Barrass J.D., Jacquier A., Rain J.-C., Beggs J.D.
    RNA 15:2161-2173(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRP8 AND SNU114.
  8. "A systematic characterization of Cwc21, the yeast ortholog of the human spliceosomal protein SRm300."
    Khanna M., Van Bakel H., Tang X., Calarco J.A., Babak T., Guo G., Emili A., Greenblatt J.F., Hughes T.R., Krogan N.J., Blencowe B.J.
    RNA 15:2174-2185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN SPLICEOSOME.

Entry informationi

Entry nameiCWC21_YEAST
AccessioniPrimary (citable) accession number: Q03375
Secondary accession number(s): D6VTA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 556 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.