Q03375 (CWC21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor CWC21

Alternative name(s):
Complexed with CEF1 protein 21

Gene names

Name:	CWC21
Ordered Locus Names:	YDR482C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. May function at or prior to the first catalytic step of splicing at the catalytic center of the spliceosome, together with ISY1. May do so by stabilizing the catalytic center or the position of the RNA substrate. Ref.7 Ref.8
Subunit structure	Associates with the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts directly with PRP8 (via SCwid domain). Interacts directly with SNU114 (via C-terminus). Ref.4 Ref.7 Ref.8
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 556 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CWC21 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from mutant phenotype Ref.8. Source: SGD
Cellular_component	U2-type spliceosomal complex Inferred from direct assay Ref.4. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TPD3	P31383	1	EBI-30409,EBI-1936

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 135	135	Pre-mRNA-splicing factor CWC21		PRO_0000123505
Regions
	Region	53 – 97	45	Interaction with PRP8
	Coiled coil	65 – 102	38	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03375 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9723E1C0A393508B	FASTA	135	15,763
	10 20 30 40 50 60 MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS HDKASRPLAV 70 80 90 100 110 120 QKQIETHMEK REIEVQVSEL RDRLEEEETL SEEQIDKKCE ALRAKLTNEW QEQQRMSSLY 130 TPRKARLTEE QHRHE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome." Grainger R.J., Barrass J.D., Jacquier A., Rain J.-C., Beggs J.D. RNA 15:2161-2173(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PRP8 AND SNU114.
[8]	"A systematic characterization of Cwc21, the yeast ortholog of the human spliceosomal protein SRm300." Khanna M., Van Bakel H., Tang X., Calarco J.A., Babak T., Guo G., Emili A., Greenblatt J.F., Hughes T.R., Krogan N.J., Blencowe B.J. RNA 15:2174-2185(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN SPLICEOSOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33050 Genomic DNA. Translation: AAB64929.1. AY557800 Genomic DNA. Translation: AAS56126.1. BK006938 Genomic DNA. Translation: DAA12315.1.
PIR	S69649.
RefSeq	NP_010770.3. NM_001180790.3.
3D structure databases
ProteinModelPortal	Q03375.
SMR	Q03375. Positions 67-110.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1847N.
IntAct	Q03375. 7 interactions.
MINT	MINT-407313.
STRING	4932.YDR482C.
Proteomic databases
PaxDb	Q03375.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR482C; YDR482C; YDR482C.
GeneID	852093.
KEGG	sce:YDR482C. sce:YDR486C.
Organism-specific databases
CYGD	YDR482c.
SGD	S000002890. CWC21.
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000065927.
KO	K12198.
OMA	RIDEECE.
OrthoDB	EOG4NPBDC.
Gene expression databases
Genevestigator	Q03375.
GermOnline	YDR482C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013170. mRNA_splic_Cwf21. [Graphical view]
Pfam	PF08312. cwf21. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970424.

Entry information

Entry name

CWC21_YEAST

Accession

Primary (citable) accession number: Q03375
Secondary accession number(s): D6VTA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program