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Q03375

- CWC21_YEAST

UniProt

Q03375 - CWC21_YEAST

Protein

Pre-mRNA-splicing factor CWC21

Gene

CWC21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in pre-mRNA splicing. May function at or prior to the first catalytic step of splicing at the catalytic center of the spliceosome, together with ISY1. May do so by stabilizing the catalytic center or the position of the RNA substrate.2 Publications

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30007-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor CWC21
    Alternative name(s):
    Complexed with CEF1 protein 21
    Gene namesi
    Name:CWC21
    Ordered Locus Names:YDR482C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR482c.
    SGDiS000002890. CWC21.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. U2-type spliceosomal complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 135135Pre-mRNA-splicing factor CWC21PRO_0000123505Add
    BLAST

    Proteomic databases

    PaxDbiQ03375.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03375.

    Interactioni

    Subunit structurei

    Associates with the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts directly with PRP8 (via SCwid domain). Interacts directly with SNU114 (via C-terminus).3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TPD3P313831EBI-30409,EBI-1936

    Protein-protein interaction databases

    BioGridi32534. 76 interactions.
    DIPiDIP-1847N.
    IntActiQ03375. 6 interactions.
    MINTiMINT-407313.
    STRINGi4932.YDR482C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03375.
    SMRiQ03375. Positions 67-110.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 9745Interaction with PRP8Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili65 – 10238Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CWC21 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000065927.
    OMAiKRESTEY.
    OrthoDBiEOG7FV43H.

    Family and domain databases

    InterProiIPR013170. mRNA_splic_Cwf21.
    [Graphical view]
    PfamiPF08312. cwf21. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03375-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS    50
    HDKASRPLAV QKQIETHMEK REIEVQVSEL RDRLEEEETL SEEQIDKKCE 100
    ALRAKLTNEW QEQQRMSSLY TPRKARLTEE QHRHE 135
    Length:135
    Mass (Da):15,763
    Last modified:November 1, 1996 - v1
    Checksum:i9723E1C0A393508B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33050 Genomic DNA. Translation: AAB64929.1.
    AY557800 Genomic DNA. Translation: AAS56126.1.
    BK006938 Genomic DNA. Translation: DAA12315.1.
    PIRiS69649.
    RefSeqiNP_010770.3. NM_001180790.3.

    Genome annotation databases

    EnsemblFungiiYDR482C; YDR482C; YDR482C.
    GeneIDi852093.
    KEGGisce:YDR482C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33050 Genomic DNA. Translation: AAB64929.1 .
    AY557800 Genomic DNA. Translation: AAS56126.1 .
    BK006938 Genomic DNA. Translation: DAA12315.1 .
    PIRi S69649.
    RefSeqi NP_010770.3. NM_001180790.3.

    3D structure databases

    ProteinModelPortali Q03375.
    SMRi Q03375. Positions 67-110.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32534. 76 interactions.
    DIPi DIP-1847N.
    IntActi Q03375. 6 interactions.
    MINTi MINT-407313.
    STRINGi 4932.YDR482C.

    Proteomic databases

    PaxDbi Q03375.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR482C ; YDR482C ; YDR482C .
    GeneIDi 852093.
    KEGGi sce:YDR482C.

    Organism-specific databases

    CYGDi YDR482c.
    SGDi S000002890. CWC21.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000065927.
    OMAi KRESTEY.
    OrthoDBi EOG7FV43H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30007-MONOMER.

    Miscellaneous databases

    NextBioi 970424.

    Gene expression databases

    Genevestigatori Q03375.

    Family and domain databases

    InterProi IPR013170. mRNA_splic_Cwf21.
    [Graphical view ]
    Pfami PF08312. cwf21. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome."
      Grainger R.J., Barrass J.D., Jacquier A., Rain J.-C., Beggs J.D.
      RNA 15:2161-2173(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PRP8 AND SNU114.
    8. "A systematic characterization of Cwc21, the yeast ortholog of the human spliceosomal protein SRm300."
      Khanna M., Van Bakel H., Tang X., Calarco J.A., Babak T., Guo G., Emili A., Greenblatt J.F., Hughes T.R., Krogan N.J., Blencowe B.J.
      RNA 15:2174-2185(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN SPLICEOSOME.

    Entry informationi

    Entry nameiCWC21_YEAST
    AccessioniPrimary (citable) accession number: Q03375
    Secondary accession number(s): D6VTA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 556 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3