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Q03375

- CWC21_YEAST

UniProt

Q03375 - CWC21_YEAST

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Protein

Pre-mRNA-splicing factor CWC21

Gene
CWC21, YDR482C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. May function at or prior to the first catalytic step of splicing at the catalytic center of the spliceosome, together with ISY1. May do so by stabilizing the catalytic center or the position of the RNA substrate.2 Publications

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30007-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC21
Alternative name(s):
Complexed with CEF1 protein 21
Gene namesi
Name:CWC21
Ordered Locus Names:YDR482C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR482c.
SGDiS000002890. CWC21.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. U2-type spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 135135Pre-mRNA-splicing factor CWC21PRO_0000123505Add
BLAST

Proteomic databases

PaxDbiQ03375.

Expressioni

Gene expression databases

GenevestigatoriQ03375.

Interactioni

Subunit structurei

Associates with the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts directly with PRP8 (via SCwid domain). Interacts directly with SNU114 (via C-terminus).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TPD3P313831EBI-30409,EBI-1936

Protein-protein interaction databases

BioGridi32534. 76 interactions.
DIPiDIP-1847N.
IntActiQ03375. 6 interactions.
MINTiMINT-407313.
STRINGi4932.YDR482C.

Structurei

3D structure databases

ProteinModelPortaliQ03375.
SMRiQ03375. Positions 67-110.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 9745Interaction with PRP8Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili65 – 10238 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the CWC21 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000065927.
OMAiKRESTEY.
OrthoDBiEOG7FV43H.

Family and domain databases

InterProiIPR013170. mRNA_splic_Cwf21.
[Graphical view]
PfamiPF08312. cwf21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03375-1 [UniParc]FASTAAdd to Basket

« Hide

MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS    50
HDKASRPLAV QKQIETHMEK REIEVQVSEL RDRLEEEETL SEEQIDKKCE 100
ALRAKLTNEW QEQQRMSSLY TPRKARLTEE QHRHE 135
Length:135
Mass (Da):15,763
Last modified:November 1, 1996 - v1
Checksum:i9723E1C0A393508B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33050 Genomic DNA. Translation: AAB64929.1.
AY557800 Genomic DNA. Translation: AAS56126.1.
BK006938 Genomic DNA. Translation: DAA12315.1.
PIRiS69649.
RefSeqiNP_010770.3. NM_001180790.3.

Genome annotation databases

EnsemblFungiiYDR482C; YDR482C; YDR482C.
GeneIDi852093.
KEGGisce:YDR482C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33050 Genomic DNA. Translation: AAB64929.1 .
AY557800 Genomic DNA. Translation: AAS56126.1 .
BK006938 Genomic DNA. Translation: DAA12315.1 .
PIRi S69649.
RefSeqi NP_010770.3. NM_001180790.3.

3D structure databases

ProteinModelPortali Q03375.
SMRi Q03375. Positions 67-110.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32534. 76 interactions.
DIPi DIP-1847N.
IntActi Q03375. 6 interactions.
MINTi MINT-407313.
STRINGi 4932.YDR482C.

Proteomic databases

PaxDbi Q03375.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR482C ; YDR482C ; YDR482C .
GeneIDi 852093.
KEGGi sce:YDR482C.

Organism-specific databases

CYGDi YDR482c.
SGDi S000002890. CWC21.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000065927.
OMAi KRESTEY.
OrthoDBi EOG7FV43H.

Enzyme and pathway databases

BioCyci YEAST:G3O-30007-MONOMER.

Miscellaneous databases

NextBioi 970424.

Gene expression databases

Genevestigatori Q03375.

Family and domain databases

InterProi IPR013170. mRNA_splic_Cwf21.
[Graphical view ]
Pfami PF08312. cwf21. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome."
    Grainger R.J., Barrass J.D., Jacquier A., Rain J.-C., Beggs J.D.
    RNA 15:2161-2173(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRP8 AND SNU114.
  8. "A systematic characterization of Cwc21, the yeast ortholog of the human spliceosomal protein SRm300."
    Khanna M., Van Bakel H., Tang X., Calarco J.A., Babak T., Guo G., Emili A., Greenblatt J.F., Hughes T.R., Krogan N.J., Blencowe B.J.
    RNA 15:2174-2185(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN SPLICEOSOME.

Entry informationi

Entry nameiCWC21_YEAST
AccessioniPrimary (citable) accession number: Q03375
Secondary accession number(s): D6VTA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 556 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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