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Protein

Down-regulator of invasive growth 2

Gene

DIG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DIG2 and DIG1 are negative regulators of the filamentation and pheromone induced mating program. DIG1 and DIG2 inhibit the transcriptional activity of STE12 by direct protein-protein interaction. DIG2 binds to the DNA binding domain (DBD) of STE12 and thus inhibits transcription when overexpressed.6 Publications

Miscellaneous

Present with 1310 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • transcription factor binding Source: SGD

GO - Biological processi

  • negative regulation of invasive growth in response to glucose limitation Source: SGD
  • negative regulation of pseudohyphal growth Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-30006-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Down-regulator of invasive growth 2
Alternative name(s):
Regulator of STE12 protein 2
Regulator of sterile twelve 2
Gene namesi
Name:DIG2
Synonyms:RST2
Ordered Locus Names:YDR480W
ORF Names:D8035.23
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR480W.
SGDiS000002888. DIG2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • Ste12p-Dig1p-Dig2p complex Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000798981 – 323Down-regulator of invasive growth 2Add BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei225PhosphoserineCombined sources1
Modified residuei266PhosphoserineCombined sources1
Modified residuei270PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by FUS3 and KSS1, in a pheromone-stimulated manner.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03373.
PRIDEiQ03373.

PTM databases

iPTMnetiQ03373.

Expressioni

Inductioni

By pheromone.1 Publication

Interactioni

Subunit structurei

Forms a complex with DIG1, STE12 and either FUS3 or KSS1. The interaction of FUS3 with STE12 depends on the presence of both DIG1 and DIG2. STE12 is lost from FUS3/DIG1/DIG2 complex after pheromone treatment. DIG1 and DIG2 have also been reported to interact with CLN1 and CLN2.

Binary interactionsi

Show more details

GO - Molecular functioni

  • transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi32532. 63 interactors.
DIPiDIP-1292N.
IntActiQ03373. 23 interactors.
MINTiMINT-405008.
STRINGi4932.YDR480W.

Structurei

3D structure databases

ProteinModelPortaliQ03373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

KOiK11242.
OrthoDBiEOG092C384J.

Sequencei

Sequence statusi: Complete.

Q03373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKEEQEDPQ QEQISTVQEN DPRNLQQLGM LLVSPGLDED RLSEKMISKI
60 70 80 90 100
KKSRDIEKNQ KLLISRLSQK EEDHSGKPPT ITTSPAEKTV PFKSLNHSLK
110 120 130 140 150
RKRVPPALNF SDIQASSHLH GSKSAPPNIT RFPQHKNSLR VRYMGRMAPT
160 170 180 190 200
NQDYHPSVAN SYMTATYPYP YTGLPPVPCY PYSSTPTQTH AYEGYYSPMY
210 220 230 240 250
PGPLYNNGII PADYHAKRKK LAGRSPHLED LTSRKRTFVS KHHNGDPIIS
260 270 280 290 300
KTDEDIECSV TKNSLSEGAS LNDDADDDND KERIIIGEIS LYDDVFKFEV
310 320
RDDKNDYMKA CETIWTEWHN LKK
Length:323
Mass (Da):36,879
Last modified:November 1, 1996 - v1
Checksum:i8E71278ED6D3A912
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64911.1.
BK006938 Genomic DNA. Translation: DAA12313.1.
PIRiS69647.
RefSeqiNP_010768.1. NM_001180788.1.

Genome annotation databases

EnsemblFungiiYDR480W; YDR480W; YDR480W.
GeneIDi852091.
KEGGisce:YDR480W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDIG2_YEAST
AccessioniPrimary (citable) accession number: Q03373
Secondary accession number(s): D6VTA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names