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Q03373 (DIG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Down-regulator of invasive growth 2
Alternative name(s):
Regulator of STE12 protein 2
Regulator of sterile twelve 2
Gene names
Name:DIG2
Synonyms:RST2
Ordered Locus Names:YDR480W
ORF Names:D8035.23
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DIG2 and DIG1 are negative regulators of the filamentation and pheromone induced mating program. DIG1 and DIG2 inhibit the transcriptional activity of STE12 by direct protein-protein interaction. DIG2 binds to the DNA binding domain (DBD) of STE12 and thus inhibits transcription when overexpressed. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Subunit structure

Forms a complex with DIG1, STE12 and either FUS3 or KSS1. The interaction of FUS3 with STE12 depends on the presence of both DIG1 and DIG2. STE12 is lost from FUS3/DIG1/DIG2 complex after pheromone treatment. DIG1 and DIG2 have also been reported to interact with CLN1 and CLN2.

Subcellular location

Nucleus.

Induction

By pheromone.

Post-translational modification

Phosphorylated by FUS3 and KSS1, in a pheromone-stimulated manner. Ref.3 Ref.8

Miscellaneous

Present with 1310 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DIG1Q030633EBI-34019,EBI-29752
KSS1P146816EBI-34019,EBI-9945
STE12P135743EBI-34019,EBI-18264

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Down-regulator of invasive growth 2
PRO_0000079898

Amino acid modifications

Modified residue341Phosphoserine Ref.10 Ref.11
Modified residue831Phosphothreonine Ref.14
Modified residue841Phosphoserine Ref.13 Ref.14
Modified residue1241Phosphoserine Ref.12
Modified residue2251Phosphoserine Ref.11 Ref.12 Ref.13 Ref.14
Modified residue2641Phosphoserine Ref.14
Modified residue2661Phosphoserine Ref.13 Ref.14
Modified residue2701Phosphoserine Ref.14

Sequences

Sequence LengthMass (Da)Tools
Q03373 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8E71278ED6D3A912

FASTA32336,879
        10         20         30         40         50         60 
MNKEEQEDPQ QEQISTVQEN DPRNLQQLGM LLVSPGLDED RLSEKMISKI KKSRDIEKNQ 

        70         80         90        100        110        120 
KLLISRLSQK EEDHSGKPPT ITTSPAEKTV PFKSLNHSLK RKRVPPALNF SDIQASSHLH 

       130        140        150        160        170        180 
GSKSAPPNIT RFPQHKNSLR VRYMGRMAPT NQDYHPSVAN SYMTATYPYP YTGLPPVPCY 

       190        200        210        220        230        240 
PYSSTPTQTH AYEGYYSPMY PGPLYNNGII PADYHAKRKK LAGRSPHLED LTSRKRTFVS 

       250        260        270        280        290        300 
KHHNGDPIIS KTDEDIECSV TKNSLSEGAS LNDDADDDND KERIIIGEIS LYDDVFKFEV 

       310        320 
RDDKNDYMKA CETIWTEWHN LKK

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae."
Cook J.G., Bardwell L., Kron S.J., Thorner J.
Genes Dev. 10:2831-2848(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH STE12 AND KSS1, PHOSPHORYLATION BY KSS1.
[4]"Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates."
Tedford K., Kim S., Sa D., Stevens K., Tyers M.
Curr. Biol. 7:228-238(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COMPLEX WITH DIG1; FUS3 AND STE12, INTERACTION WITH CLN1 AND CLN2.
[5]"Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK."
Bardwell L., Cook J.G., Voora D., Baggott D.M., Martinez A.R., Thorner J.
Genes Dev. 12:2887-2898(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COMPLEX WITH DIG1; KSS1 AND STE12.
[6]"Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins."
Bardwell L., Cook J.G., Zhu-Shimoni J.X., Voora D., Thorner J.
Proc. Natl. Acad. Sci. U.S.A. 95:15400-15405(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms."
Olson K.A., Nelson C., Tai G., Hung W., Yong C., Astell C., Sadowski I.
Mol. Cell. Biol. 20:4199-4209(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH STE12.
[8]"Rst1 and Rst2 are required for the a/alpha diploid cell type in yeast."
Gelli A.
Mol. Microbiol. 46:845-854(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION BY FUS3, REPRESSION OF HAPLOID SPECIFIC AND A-SPECIFIC GENES.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, MASS SPECTROMETRY.
Strain: YAL6B.
[11]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34 AND SER-225, MASS SPECTROMETRY.
Strain: ADR376.
[12]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-225, MASS SPECTROMETRY.
[13]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84; SER-225 AND SER-266, MASS SPECTROMETRY.
[14]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-83; SER-84; SER-225; SER-264; SER-266 AND SER-270, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U33050 Genomic DNA. Translation: AAB64911.1.
BK006938 Genomic DNA. Translation: DAA12313.1.
PIRS69647.
RefSeqNP_010768.1. NM_001180788.1.

3D structure databases

ProteinModelPortalQ03373.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1292N.
IntActQ03373. 15 interactions.
MINTMINT-405008.
STRING4932.YDR480W.

Proteomic databases

PaxDbQ03373.
PeptideAtlasQ03373.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR480W; YDR480W; YDR480W.
GeneID852091.
KEGGsce:YDR480W.

Organism-specific databases

CYGDYDR480w.
SGDS000002888. DIG2.

Phylogenomic databases

eggNOGNOG139860.
KOK11242.
OrthoDBEOG41ZJMG.

Gene expression databases

GenevestigatorQ03373.
GermOnlineYDR480W. Saccharomyces cerevisiae.

Family and domain databases

ProtoNetSearch...

Other

NextBio970418.

Entry information

Entry nameDIG2_YEAST
AccessionPrimary (citable) accession number: Q03373
Secondary accession number(s): D6VTA3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents