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Q03358

- MSX2_MOUSE

UniProt

Q03358 - MSX2_MOUSE

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Protein

Homeobox protein MSX-2

Gene
Msx2, Hox-8.1, Msx-2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi142 – 20160Homeobox1 PublicationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. protein binding Source: IntAct
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Source: Ensembl
  5. transcription cofactor activity Source: BHF-UCL
  6. transcription factor binding Source: MGI
  7. transcription factor binding transcription factor activity Source: MGI
  8. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. activation of meiosis Source: MGI
  2. anagen Source: MGI
  3. anterior/posterior pattern specification Source: MGI
  4. BMP signaling pathway Source: MGI
  5. BMP signaling pathway involved in heart development Source: MGI
  6. bone morphogenesis Source: MGI
  7. bone trabecula formation Source: MGI
  8. branching involved in mammary gland duct morphogenesis Source: MGI
  9. cartilage development Source: MGI
  10. cellular response to estradiol stimulus Source: MGI
  11. cellular response to growth factor stimulus Source: MGI
  12. chondrocyte development Source: MGI
  13. embryonic digit morphogenesis Source: MGI
  14. embryonic forelimb morphogenesis Source: MGI
  15. embryonic hindlimb morphogenesis Source: MGI
  16. embryonic limb morphogenesis Source: MGI
  17. embryonic nail plate morphogenesis Source: MGI
  18. enamel mineralization Source: MGI
  19. endochondral bone growth Source: MGI
  20. epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
  21. frontal suture morphogenesis Source: MGI
  22. mammary gland epithelium development Source: MGI
  23. negative regulation of apoptotic process Source: MGI
  24. negative regulation of cell proliferation Source: MGI
  25. negative regulation of CREB transcription factor activity Source: MGI
  26. negative regulation of fat cell differentiation Source: MGI
  27. negative regulation of keratinocyte differentiation Source: MGI
  28. negative regulation of transcription, DNA-templated Source: UniProtKB
  29. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  30. negative regulation of transcription regulatory region DNA binding Source: BHF-UCL
  31. odontogenesis Source: MGI
  32. ossification Source: MGI
  33. osteoblast development Source: MGI
  34. osteoblast differentiation Source: UniProtKB
  35. outflow tract morphogenesis Source: MGI
  36. outflow tract septum morphogenesis Source: MGI
  37. positive regulation of BMP signaling pathway Source: MGI
  38. positive regulation of catagen Source: MGI
  39. positive regulation of mesenchymal cell apoptotic process Source: MGI
  40. positive regulation of osteoblast differentiation Source: MGI
  41. regulation of apoptotic process Source: MGI
  42. signal transduction involved in regulation of gene expression Source: MGI
  43. stem cell differentiation Source: MGI
  44. wound healing Source: MGI
  45. wound healing, spreading of epidermal cells Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Osteogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein MSX-2
Alternative name(s):
Homeobox protein Hox-8-1
Gene namesi
Name:Msx2
Synonyms:Hox-8.1, Msx-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:97169. Msx2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Homeobox protein MSX-2PRO_0000049100Add
BLAST

Proteomic databases

PRIDEiQ03358.

PTM databases

PhosphoSiteiQ03358.

Expressioni

Tissue specificityi

Expressed in mesenchymal tissue in the developing spinal cord and limbs.

Inductioni

Up-regulated by BMP2.1 Publication

Gene expression databases

ArrayExpressiQ03358.
BgeeiQ03358.
CleanExiMM_MSX2.
GenevestigatoriQ03358.

Interactioni

Subunit structurei

Interacts with MINT. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80) By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Maged1Q9QYH65EBI-1801354,EBI-1801274
NdnP252333EBI-1801354,EBI-1801080

Protein-protein interaction databases

BioGridi201537. 4 interactions.
IntActiQ03358. 3 interactions.
STRINGi10090.ENSMUSP00000021922.

Structurei

3D structure databases

ProteinModelPortaliQ03358.
SMRiQ03358. Positions 143-200.

Family & Domainsi

Sequence similaritiesi

Belongs to the Msh homeobox family.

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG298790.
HOGENOMiHOG000231922.
HOVERGENiHBG005205.
InParanoidiQ03358.
KOiK09341.
OMAiPPKEMSS.
OrthoDBiEOG70GMGK.
PhylomeDBiQ03358.
TreeFamiTF350699.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03358-1 [UniParc]FASTAAdd to Basket

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MASPTKGGDL FSSDEEGPAV LAGPGPGPGG AEGSAEERRV KVSSLPFSVE    50
ALMSDKKPPK ESPAVPPDCA SAGAVLRPLL LPGHGVRDAH SPGPLVKPFE 100
TASVKSENSE DGAPWIQEPG RYSPPPRHMS PTTCTLRKHK TNRKPRTPFT 150
TSQLLALERK FRQKQYLSIA ERAEFSSSLN LTETQVKIWF QNRRAKAKRL 200
QEAELEKLKM AAKPMLPSGF SLPFPINSPL QAASIYGASY PFHRPVLPIP 250
PVGLYATPVG YGMYHLS 267
Length:267
Mass (Da):28,915
Last modified:June 1, 1994 - v2
Checksum:iF2F4528D6E9C951E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111F → FF in CAA41945. 1 Publication
Sequence conflicti89 – 891A → R in AAC60657. 1 Publication
Sequence conflicti263 – 2631M → I in CAA41945. 1 Publication
Sequence conflicti266 – 2661L → V in CAA41945. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11739, L11738 Unassigned DNA. Translation: AAA03477.1.
S60460, S60698 Genomic DNA. Translation: AAC60657.2.
X59252 mRNA. Translation: CAA41945.1.
M38576 Genomic DNA. Translation: AAA37824.1.
CCDSiCCDS26522.1.
PIRiA46122.
PS0410.
S18814.
RefSeqiNP_038629.2. NM_013601.2.
UniGeneiMm.1763.

Genome annotation databases

EnsembliENSMUST00000021922; ENSMUSP00000021922; ENSMUSG00000021469.
GeneIDi17702.
KEGGimmu:17702.
UCSCiuc007qnw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L11739 , L11738 Unassigned DNA. Translation: AAA03477.1 .
S60460 , S60698 Genomic DNA. Translation: AAC60657.2 .
X59252 mRNA. Translation: CAA41945.1 .
M38576 Genomic DNA. Translation: AAA37824.1 .
CCDSi CCDS26522.1.
PIRi A46122.
PS0410.
S18814.
RefSeqi NP_038629.2. NM_013601.2.
UniGenei Mm.1763.

3D structure databases

ProteinModelPortali Q03358.
SMRi Q03358. Positions 143-200.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201537. 4 interactions.
IntActi Q03358. 3 interactions.
STRINGi 10090.ENSMUSP00000021922.

PTM databases

PhosphoSitei Q03358.

Proteomic databases

PRIDEi Q03358.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021922 ; ENSMUSP00000021922 ; ENSMUSG00000021469 .
GeneIDi 17702.
KEGGi mmu:17702.
UCSCi uc007qnw.1. mouse.

Organism-specific databases

CTDi 4488.
MGIi MGI:97169. Msx2.

Phylogenomic databases

eggNOGi NOG298790.
HOGENOMi HOG000231922.
HOVERGENi HBG005205.
InParanoidi Q03358.
KOi K09341.
OMAi PPKEMSS.
OrthoDBi EOG70GMGK.
PhylomeDBi Q03358.
TreeFami TF350699.

Miscellaneous databases

NextBioi 292298.
PROi Q03358.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q03358.
Bgeei Q03358.
CleanExi MM_MSX2.
Genevestigatori Q03358.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
[Graphical view ]
PRINTSi PR00024. HOMEOBOX.
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Msh-like homeobox genes define domains in the developing vertebrate eye."
    Monaghan A.P., Davidson D.R., Sime C., Graham E., Baldock R., Bhattacharya S.S., Hill R.E.
    Development 112:1053-1061(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Genomic structure, chromosomal location, and evolution of the mouse Hox 8 gene."
    Bell J.R., Noveen A., Liu Y.H., Ma L., Dobias S., Kundu R., Luo W., Xia Y.
    Genomics 16:123-131(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning and evolutionary analysis of msh-like homeobox genes from mouse, zebrafish and ascidian."
    Holland P.W.H.
    Gene 98:253-257(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-202.
  4. "Position-dependent expression of two related homeobox genes in developing vertebrate limbs."
    Davidson D.R., Crawley A., Hill R.E., Tickle C.
    Nature 352:429-431(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The RRM domain of MINT, a novel msx2 binding protein, recognizes and regulates the rat osteocalcin promoter."
    Newberry E.P., Latifi T., Towler D.A.
    Biochemistry 38:10678-10690(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MINT.
  6. "Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2."
    Kim Y.J., Lee M.H., Wozney J.M., Cho J.Y., Ryoo H.M.
    J. Biol. Chem. 279:50773-50780(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DNA-BINDING.

Entry informationi

Entry nameiMSX2_MOUSE
AccessioniPrimary (citable) accession number: Q03358
Secondary accession number(s): Q63856
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi