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Q03358 (MSX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein MSX-2
Alternative name(s):
Homeobox protein Hox-8-1
Gene names
Name:Msx2
Synonyms:Hox-8.1, Msx-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter. Ref.4 Ref.6

Subunit structure

Interacts with MINT. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80) By similarity. Ref.5

Subcellular location

Nucleus.

Tissue specificity

Expressed in mesenchymal tissue in the developing spinal cord and limbs.

Induction

Up-regulated by BMP2. Ref.6

Sequence similarities

Belongs to the Msh homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processOsteogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
Repressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred from direct assay PubMed 18590716. Source: MGI

BMP signaling pathway involved in heart development

Inferred from genetic interaction PubMed 18667074. Source: MGI

activation of meiosis

Inferred from physical interaction PubMed 22071108. Source: MGI

anagen

Inferred from mutant phenotype PubMed 11744114. Source: MGI

anterior/posterior pattern specification

Inferred from genetic interaction PubMed 21465616. Source: MGI

bone morphogenesis

Inferred from genetic interaction PubMed 17693062. Source: MGI

bone trabecula formation

Inferred from mutant phenotype PubMed 10742104. Source: MGI

branching involved in mammary gland duct morphogenesis

Inferred from mutant phenotype PubMed 10742104. Source: MGI

cartilage development

Inferred from mutant phenotype PubMed 10742104PubMed 11668602. Source: MGI

cellular response to estradiol stimulus

Inferred from direct assay PubMed 8787747. Source: MGI

cellular response to growth factor stimulus

Inferred from direct assay PubMed 7848824. Source: MGI

chondrocyte development

Inferred from mutant phenotype PubMed 10742104. Source: MGI

embryonic digit morphogenesis

Inferred from genetic interaction PubMed 21465616. Source: MGI

embryonic forelimb morphogenesis

Inferred from genetic interaction PubMed 21465616. Source: MGI

embryonic hindlimb morphogenesis

Inferred from genetic interaction PubMed 21465616. Source: MGI

embryonic limb morphogenesis

Inferred from genetic interaction PubMed 15930102PubMed 19422820. Source: MGI

embryonic nail plate morphogenesis

Inferred from genetic interaction PubMed 21465616. Source: MGI

enamel mineralization

Inferred from mutant phenotype PubMed 10742104. Source: MGI

endochondral bone growth

Inferred from mutant phenotype PubMed 10742104. Source: MGI

epithelial to mesenchymal transition involved in endocardial cushion formation

Inferred from genetic interaction PubMed 18667074. Source: MGI

frontal suture morphogenesis

Inferred from mutant phenotype PubMed 10742104. Source: MGI

mammary gland epithelium development

Inferred from genetic interaction PubMed 10742104. Source: MGI

negative regulation of CREB transcription factor activity

Inferred from direct assay PubMed 15175325. Source: MGI

negative regulation of apoptotic process

Inferred from genetic interaction PubMed 17601530. Source: MGI

negative regulation of cell proliferation

Inferred from genetic interaction PubMed 17601530. Source: MGI

negative regulation of fat cell differentiation

Inferred from direct assay PubMed 15175325. Source: MGI

negative regulation of keratinocyte differentiation

Inferred from mutant phenotype PubMed 19769717. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 20843790. Source: BHF-UCL

negative regulation of transcription regulatory region DNA binding

Inferred from direct assay PubMed 20843790. Source: BHF-UCL

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.6. Source: UniProtKB

odontogenesis

Inferred from mutant phenotype PubMed 10742104. Source: MGI

ossification

Inferred from mutant phenotype PubMed 10742104. Source: MGI

osteoblast development

Inferred from mutant phenotype PubMed 10742104. Source: MGI

osteoblast differentiation

Inferred from direct assay Ref.6. Source: UniProtKB

outflow tract morphogenesis

Inferred from genetic interaction PubMed 17601530. Source: MGI

outflow tract septum morphogenesis

Inferred from genetic interaction PubMed 17601530. Source: MGI

positive regulation of BMP signaling pathway

Inferred from genetic interaction PubMed 18667074. Source: MGI

positive regulation of catagen

Inferred from mutant phenotype PubMed 10742104. Source: MGI

positive regulation of mesenchymal cell apoptotic process

Inferred from genetic interaction PubMed 19422820. Source: MGI

positive regulation of osteoblast differentiation

Inferred from direct assay PubMed 15175325. Source: MGI

regulation of apoptotic process

Inferred from direct assay PubMed 18729207. Source: MGI

signal transduction involved in regulation of gene expression

Inferred from genetic interaction PubMed 21465616. Source: MGI

stem cell differentiation

Inferred from genetic interaction PubMed 17693062. Source: MGI

wound healing

Inferred from mutant phenotype PubMed 19769717. Source: MGI

wound healing, spreading of epidermal cells

Inferred from mutant phenotype PubMed 19769717. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Inferred from physical interaction PubMed 18285513. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 9265625. Source: MGI

RNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 11084035PubMed 11959851PubMed 15272023. Source: IntAct

transcription cofactor activity

Inferred from direct assay PubMed 20843790. Source: BHF-UCL

transcription factor binding

Inferred from physical interaction PubMed 9265625. Source: MGI

transcription factor binding transcription factor activity

Inferred from direct assay PubMed 9265625. Source: MGI

transcription regulatory region DNA binding

Inferred from direct assay Ref.6. Source: UniProtKB

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267Homeobox protein MSX-2
PRO_0000049100

Regions

DNA binding142 – 20160Homeobox Ref.6

Experimental info

Sequence conflict111F → FF in CAA41945. Ref.1
Sequence conflict891A → R in AAC60657. Ref.2
Sequence conflict2631M → I in CAA41945. Ref.1
Sequence conflict2661L → V in CAA41945. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q03358 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: F2F4528D6E9C951E

FASTA26728,915
        10         20         30         40         50         60 
MASPTKGGDL FSSDEEGPAV LAGPGPGPGG AEGSAEERRV KVSSLPFSVE ALMSDKKPPK 

        70         80         90        100        110        120 
ESPAVPPDCA SAGAVLRPLL LPGHGVRDAH SPGPLVKPFE TASVKSENSE DGAPWIQEPG 

       130        140        150        160        170        180 
RYSPPPRHMS PTTCTLRKHK TNRKPRTPFT TSQLLALERK FRQKQYLSIA ERAEFSSSLN 

       190        200        210        220        230        240 
LTETQVKIWF QNRRAKAKRL QEAELEKLKM AAKPMLPSGF SLPFPINSPL QAASIYGASY 

       250        260 
PFHRPVLPIP PVGLYATPVG YGMYHLS 

« Hide

References

[1]"The Msh-like homeobox genes define domains in the developing vertebrate eye."
Monaghan A.P., Davidson D.R., Sime C., Graham E., Baldock R., Bhattacharya S.S., Hill R.E.
Development 112:1053-1061(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"Genomic structure, chromosomal location, and evolution of the mouse Hox 8 gene."
Bell J.R., Noveen A., Liu Y.H., Ma L., Dobias S., Kundu R., Luo W., Xia Y.
Genomics 16:123-131(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning and evolutionary analysis of msh-like homeobox genes from mouse, zebrafish and ascidian."
Holland P.W.H.
Gene 98:253-257(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-202.
[4]"Position-dependent expression of two related homeobox genes in developing vertebrate limbs."
Davidson D.R., Crawley A., Hill R.E., Tickle C.
Nature 352:429-431(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"The RRM domain of MINT, a novel msx2 binding protein, recognizes and regulates the rat osteocalcin promoter."
Newberry E.P., Latifi T., Towler D.A.
Biochemistry 38:10678-10690(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MINT.
[6]"Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2."
Kim Y.J., Lee M.H., Wozney J.M., Cho J.Y., Ryoo H.M.
J. Biol. Chem. 279:50773-50780(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, DNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11739, L11738 Unassigned DNA. Translation: AAA03477.1.
S60460, S60698 Genomic DNA. Translation: AAC60657.2.
X59252 mRNA. Translation: CAA41945.1.
M38576 Genomic DNA. Translation: AAA37824.1.
CCDSCCDS26522.1.
PIRA46122.
PS0410.
S18814.
RefSeqNP_038629.2. NM_013601.2.
UniGeneMm.1763.

3D structure databases

ProteinModelPortalQ03358.
SMRQ03358. Positions 143-200.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201537. 4 interactions.
IntActQ03358. 3 interactions.
STRING10090.ENSMUSP00000021922.

PTM databases

PhosphoSiteQ03358.

Proteomic databases

PRIDEQ03358.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021922; ENSMUSP00000021922; ENSMUSG00000021469.
GeneID17702.
KEGGmmu:17702.
UCSCuc007qnw.1. mouse.

Organism-specific databases

CTD4488.
MGIMGI:97169. Msx2.

Phylogenomic databases

eggNOGNOG298790.
HOGENOMHOG000231922.
HOVERGENHBG005205.
InParanoidQ03358.
KOK09341.
OMAPPKEMSS.
OrthoDBEOG70GMGK.
PhylomeDBQ03358.
TreeFamTF350699.

Gene expression databases

ArrayExpressQ03358.
BgeeQ03358.
CleanExMM_MSX2.
GenevestigatorQ03358.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292298.
PROQ03358.
SOURCESearch...

Entry information

Entry nameMSX2_MOUSE
AccessionPrimary (citable) accession number: Q03358
Secondary accession number(s): Q63856
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot