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Q03358

- MSX2_MOUSE

UniProt

Q03358 - MSX2_MOUSE

Protein

Homeobox protein MSX-2

Gene

Msx2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi142 – 20160HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: IntAct
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Source: Ensembl
    5. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    6. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
    7. transcription cofactor activity Source: BHF-UCL
    8. transcription factor binding Source: MGI
    9. transcription factor binding transcription factor activity Source: MGI
    10. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. activation of meiosis Source: MGI
    2. anagen Source: MGI
    3. anterior/posterior pattern specification Source: MGI
    4. BMP signaling pathway Source: MGI
    5. BMP signaling pathway involved in heart development Source: MGI
    6. bone morphogenesis Source: MGI
    7. bone trabecula formation Source: MGI
    8. branching involved in mammary gland duct morphogenesis Source: MGI
    9. cartilage development Source: MGI
    10. cellular response to estradiol stimulus Source: MGI
    11. cellular response to growth factor stimulus Source: MGI
    12. chondrocyte development Source: MGI
    13. embryonic digit morphogenesis Source: MGI
    14. embryonic forelimb morphogenesis Source: MGI
    15. embryonic hindlimb morphogenesis Source: MGI
    16. embryonic limb morphogenesis Source: MGI
    17. embryonic nail plate morphogenesis Source: MGI
    18. enamel mineralization Source: MGI
    19. endochondral bone growth Source: MGI
    20. epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
    21. frontal suture morphogenesis Source: MGI
    22. mammary gland epithelium development Source: MGI
    23. negative regulation of apoptotic process Source: MGI
    24. negative regulation of cell proliferation Source: MGI
    25. negative regulation of CREB transcription factor activity Source: MGI
    26. negative regulation of fat cell differentiation Source: MGI
    27. negative regulation of keratinocyte differentiation Source: MGI
    28. negative regulation of transcription, DNA-templated Source: UniProtKB
    29. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    30. negative regulation of transcription regulatory region DNA binding Source: BHF-UCL
    31. odontogenesis Source: MGI
    32. ossification Source: MGI
    33. osteoblast development Source: MGI
    34. osteoblast differentiation Source: UniProtKB
    35. outflow tract morphogenesis Source: MGI
    36. outflow tract septum morphogenesis Source: MGI
    37. positive regulation of BMP signaling pathway Source: MGI
    38. positive regulation of catagen Source: MGI
    39. positive regulation of mesenchymal cell apoptotic process Source: MGI
    40. positive regulation of osteoblast differentiation Source: MGI
    41. regulation of apoptotic process Source: MGI
    42. signal transduction involved in regulation of gene expression Source: MGI
    43. stem cell differentiation Source: MGI
    44. wound healing Source: MGI
    45. wound healing, spreading of epidermal cells Source: MGI

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Osteogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein MSX-2
    Alternative name(s):
    Homeobox protein Hox-8-1
    Gene namesi
    Name:Msx2
    Synonyms:Hox-8.1, Msx-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:97169. Msx2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: UniProtKB-SubCell
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 267267Homeobox protein MSX-2PRO_0000049100Add
    BLAST

    Proteomic databases

    PRIDEiQ03358.

    PTM databases

    PhosphoSiteiQ03358.

    Expressioni

    Tissue specificityi

    Expressed in mesenchymal tissue in the developing spinal cord and limbs.

    Inductioni

    Up-regulated by BMP2.1 Publication

    Gene expression databases

    ArrayExpressiQ03358.
    BgeeiQ03358.
    CleanExiMM_MSX2.
    GenevestigatoriQ03358.

    Interactioni

    Subunit structurei

    Interacts with MINT. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80) By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Maged1Q9QYH65EBI-1801354,EBI-1801274
    NdnP252333EBI-1801354,EBI-1801080

    Protein-protein interaction databases

    BioGridi201537. 4 interactions.
    IntActiQ03358. 3 interactions.
    STRINGi10090.ENSMUSP00000021922.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03358.
    SMRiQ03358. Positions 143-200.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Msh homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG298790.
    HOGENOMiHOG000231922.
    HOVERGENiHBG005205.
    InParanoidiQ03358.
    KOiK09341.
    OMAiPPKEMSS.
    OrthoDBiEOG70GMGK.
    PhylomeDBiQ03358.
    TreeFamiTF350699.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    PRINTSiPR00024. HOMEOBOX.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03358-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPTKGGDL FSSDEEGPAV LAGPGPGPGG AEGSAEERRV KVSSLPFSVE    50
    ALMSDKKPPK ESPAVPPDCA SAGAVLRPLL LPGHGVRDAH SPGPLVKPFE 100
    TASVKSENSE DGAPWIQEPG RYSPPPRHMS PTTCTLRKHK TNRKPRTPFT 150
    TSQLLALERK FRQKQYLSIA ERAEFSSSLN LTETQVKIWF QNRRAKAKRL 200
    QEAELEKLKM AAKPMLPSGF SLPFPINSPL QAASIYGASY PFHRPVLPIP 250
    PVGLYATPVG YGMYHLS 267
    Length:267
    Mass (Da):28,915
    Last modified:June 1, 1994 - v2
    Checksum:iF2F4528D6E9C951E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111F → FF in CAA41945. (PubMed:1682128)Curated
    Sequence conflicti89 – 891A → R in AAC60657. (PubMed:8098007)Curated
    Sequence conflicti263 – 2631M → I in CAA41945. (PubMed:1682128)Curated
    Sequence conflicti266 – 2661L → V in CAA41945. (PubMed:1682128)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11739, L11738 Unassigned DNA. Translation: AAA03477.1.
    S60460, S60698 Genomic DNA. Translation: AAC60657.2.
    X59252 mRNA. Translation: CAA41945.1.
    M38576 Genomic DNA. Translation: AAA37824.1.
    CCDSiCCDS26522.1.
    PIRiA46122.
    PS0410.
    S18814.
    RefSeqiNP_038629.2. NM_013601.2.
    UniGeneiMm.1763.

    Genome annotation databases

    EnsembliENSMUST00000021922; ENSMUSP00000021922; ENSMUSG00000021469.
    GeneIDi17702.
    KEGGimmu:17702.
    UCSCiuc007qnw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L11739 , L11738 Unassigned DNA. Translation: AAA03477.1 .
    S60460 , S60698 Genomic DNA. Translation: AAC60657.2 .
    X59252 mRNA. Translation: CAA41945.1 .
    M38576 Genomic DNA. Translation: AAA37824.1 .
    CCDSi CCDS26522.1.
    PIRi A46122.
    PS0410.
    S18814.
    RefSeqi NP_038629.2. NM_013601.2.
    UniGenei Mm.1763.

    3D structure databases

    ProteinModelPortali Q03358.
    SMRi Q03358. Positions 143-200.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201537. 4 interactions.
    IntActi Q03358. 3 interactions.
    STRINGi 10090.ENSMUSP00000021922.

    PTM databases

    PhosphoSitei Q03358.

    Proteomic databases

    PRIDEi Q03358.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021922 ; ENSMUSP00000021922 ; ENSMUSG00000021469 .
    GeneIDi 17702.
    KEGGi mmu:17702.
    UCSCi uc007qnw.1. mouse.

    Organism-specific databases

    CTDi 4488.
    MGIi MGI:97169. Msx2.

    Phylogenomic databases

    eggNOGi NOG298790.
    HOGENOMi HOG000231922.
    HOVERGENi HBG005205.
    InParanoidi Q03358.
    KOi K09341.
    OMAi PPKEMSS.
    OrthoDBi EOG70GMGK.
    PhylomeDBi Q03358.
    TreeFami TF350699.

    Miscellaneous databases

    NextBioi 292298.
    PROi Q03358.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q03358.
    Bgeei Q03358.
    CleanExi MM_MSX2.
    Genevestigatori Q03358.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    PRINTSi PR00024. HOMEOBOX.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Msh-like homeobox genes define domains in the developing vertebrate eye."
      Monaghan A.P., Davidson D.R., Sime C., Graham E., Baldock R., Bhattacharya S.S., Hill R.E.
      Development 112:1053-1061(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "Genomic structure, chromosomal location, and evolution of the mouse Hox 8 gene."
      Bell J.R., Noveen A., Liu Y.H., Ma L., Dobias S., Kundu R., Luo W., Xia Y.
      Genomics 16:123-131(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Cloning and evolutionary analysis of msh-like homeobox genes from mouse, zebrafish and ascidian."
      Holland P.W.H.
      Gene 98:253-257(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-202.
    4. "Position-dependent expression of two related homeobox genes in developing vertebrate limbs."
      Davidson D.R., Crawley A., Hill R.E., Tickle C.
      Nature 352:429-431(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "The RRM domain of MINT, a novel msx2 binding protein, recognizes and regulates the rat osteocalcin promoter."
      Newberry E.P., Latifi T., Towler D.A.
      Biochemistry 38:10678-10690(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MINT.
    6. "Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2."
      Kim Y.J., Lee M.H., Wozney J.M., Cho J.Y., Ryoo H.M.
      J. Biol. Chem. 279:50773-50780(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, DNA-BINDING.

    Entry informationi

    Entry nameiMSX2_MOUSE
    AccessioniPrimary (citable) accession number: Q03358
    Secondary accession number(s): Q63856
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3