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Protein

Homeobox protein MSX-2

Gene

Msx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi142 – 20160HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity Source: Ensembl
  4. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
  6. sequence-specific DNA binding Source: MGI
  7. transcription cofactor activity Source: BHF-UCL
  8. transcription factor binding Source: MGI
  9. transcription factor binding transcription factor activity Source: MGI
  10. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. activation of meiosis Source: MGI
  2. anterior/posterior pattern specification Source: MGI
  3. BMP signaling pathway Source: MGI
  4. BMP signaling pathway involved in heart development Source: MGI
  5. bone morphogenesis Source: MGI
  6. bone trabecula formation Source: MGI
  7. branching involved in mammary gland duct morphogenesis Source: MGI
  8. cartilage development Source: MGI
  9. cellular response to estradiol stimulus Source: MGI
  10. cellular response to growth factor stimulus Source: MGI
  11. chondrocyte development Source: MGI
  12. embryonic digit morphogenesis Source: MGI
  13. embryonic forelimb morphogenesis Source: MGI
  14. embryonic hindlimb morphogenesis Source: MGI
  15. embryonic limb morphogenesis Source: MGI
  16. embryonic nail plate morphogenesis Source: MGI
  17. enamel mineralization Source: MGI
  18. endochondral bone growth Source: MGI
  19. epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
  20. frontal suture morphogenesis Source: MGI
  21. mammary gland epithelium development Source: MGI
  22. negative regulation of apoptotic process Source: MGI
  23. negative regulation of cell proliferation Source: MGI
  24. negative regulation of CREB transcription factor activity Source: MGI
  25. negative regulation of fat cell differentiation Source: MGI
  26. negative regulation of keratinocyte differentiation Source: MGI
  27. negative regulation of transcription, DNA-templated Source: UniProtKB
  28. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  29. negative regulation of transcription regulatory region DNA binding Source: BHF-UCL
  30. odontogenesis Source: MGI
  31. ossification Source: MGI
  32. osteoblast development Source: MGI
  33. osteoblast differentiation Source: UniProtKB
  34. outflow tract morphogenesis Source: MGI
  35. outflow tract septum morphogenesis Source: MGI
  36. positive regulation of BMP signaling pathway Source: MGI
  37. positive regulation of catagen Source: MGI
  38. positive regulation of mesenchymal cell apoptotic process Source: MGI
  39. positive regulation of osteoblast differentiation Source: MGI
  40. regulation of apoptotic process Source: MGI
  41. signal transduction involved in regulation of gene expression Source: MGI
  42. stem cell differentiation Source: MGI
  43. wound healing Source: MGI
  44. wound healing, spreading of epidermal cells Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Osteogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein MSX-2
Alternative name(s):
Homeobox protein Hox-8-1
Gene namesi
Name:Msx2
Synonyms:Hox-8.1, Msx-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:97169. Msx2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Homeobox protein MSX-2PRO_0000049100Add
BLAST

Proteomic databases

PRIDEiQ03358.

PTM databases

PhosphoSiteiQ03358.

Expressioni

Tissue specificityi

Expressed in mesenchymal tissue in the developing spinal cord and limbs.

Inductioni

Up-regulated by BMP2.1 Publication

Gene expression databases

BgeeiQ03358.
CleanExiMM_MSX2.
ExpressionAtlasiQ03358. baseline and differential.
GenevestigatoriQ03358.

Interactioni

Subunit structurei

Interacts with MINT. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Maged1Q9QYH65EBI-1801354,EBI-1801274
NdnP252333EBI-1801354,EBI-1801080

Protein-protein interaction databases

BioGridi201537. 4 interactions.
IntActiQ03358. 3 interactions.
STRINGi10090.ENSMUSP00000021922.

Structurei

3D structure databases

ProteinModelPortaliQ03358.
SMRiQ03358. Positions 143-200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Msh homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG298790.
HOGENOMiHOG000231922.
HOVERGENiHBG005205.
InParanoidiQ03358.
KOiK09341.
OMAiPPKEMSS.
OrthoDBiEOG70GMGK.
PhylomeDBiQ03358.
TreeFamiTF350699.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPTKGGDL FSSDEEGPAV LAGPGPGPGG AEGSAEERRV KVSSLPFSVE
60 70 80 90 100
ALMSDKKPPK ESPAVPPDCA SAGAVLRPLL LPGHGVRDAH SPGPLVKPFE
110 120 130 140 150
TASVKSENSE DGAPWIQEPG RYSPPPRHMS PTTCTLRKHK TNRKPRTPFT
160 170 180 190 200
TSQLLALERK FRQKQYLSIA ERAEFSSSLN LTETQVKIWF QNRRAKAKRL
210 220 230 240 250
QEAELEKLKM AAKPMLPSGF SLPFPINSPL QAASIYGASY PFHRPVLPIP
260
PVGLYATPVG YGMYHLS
Length:267
Mass (Da):28,915
Last modified:June 1, 1994 - v2
Checksum:iF2F4528D6E9C951E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111F → FF in CAA41945 (PubMed:1682128).Curated
Sequence conflicti89 – 891A → R in AAC60657 (PubMed:8098007).Curated
Sequence conflicti263 – 2631M → I in CAA41945 (PubMed:1682128).Curated
Sequence conflicti266 – 2661L → V in CAA41945 (PubMed:1682128).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11739, L11738 Unassigned DNA. Translation: AAA03477.1.
S60460, S60698 Genomic DNA. Translation: AAC60657.2.
X59252 mRNA. Translation: CAA41945.1.
M38576 Genomic DNA. Translation: AAA37824.1.
CCDSiCCDS26522.1.
PIRiA46122.
PS0410.
S18814.
RefSeqiNP_038629.2. NM_013601.2.
UniGeneiMm.1763.

Genome annotation databases

EnsembliENSMUST00000021922; ENSMUSP00000021922; ENSMUSG00000021469.
GeneIDi17702.
KEGGimmu:17702.
UCSCiuc007qnw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11739, L11738 Unassigned DNA. Translation: AAA03477.1.
S60460, S60698 Genomic DNA. Translation: AAC60657.2.
X59252 mRNA. Translation: CAA41945.1.
M38576 Genomic DNA. Translation: AAA37824.1.
CCDSiCCDS26522.1.
PIRiA46122.
PS0410.
S18814.
RefSeqiNP_038629.2. NM_013601.2.
UniGeneiMm.1763.

3D structure databases

ProteinModelPortaliQ03358.
SMRiQ03358. Positions 143-200.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201537. 4 interactions.
IntActiQ03358. 3 interactions.
STRINGi10090.ENSMUSP00000021922.

PTM databases

PhosphoSiteiQ03358.

Proteomic databases

PRIDEiQ03358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021922; ENSMUSP00000021922; ENSMUSG00000021469.
GeneIDi17702.
KEGGimmu:17702.
UCSCiuc007qnw.1. mouse.

Organism-specific databases

CTDi4488.
MGIiMGI:97169. Msx2.

Phylogenomic databases

eggNOGiNOG298790.
HOGENOMiHOG000231922.
HOVERGENiHBG005205.
InParanoidiQ03358.
KOiK09341.
OMAiPPKEMSS.
OrthoDBiEOG70GMGK.
PhylomeDBiQ03358.
TreeFamiTF350699.

Miscellaneous databases

NextBioi292298.
PROiQ03358.
SOURCEiSearch...

Gene expression databases

BgeeiQ03358.
CleanExiMM_MSX2.
ExpressionAtlasiQ03358. baseline and differential.
GenevestigatoriQ03358.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Msh-like homeobox genes define domains in the developing vertebrate eye."
    Monaghan A.P., Davidson D.R., Sime C., Graham E., Baldock R., Bhattacharya S.S., Hill R.E.
    Development 112:1053-1061(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Genomic structure, chromosomal location, and evolution of the mouse Hox 8 gene."
    Bell J.R., Noveen A., Liu Y.H., Ma L., Dobias S., Kundu R., Luo W., Xia Y.
    Genomics 16:123-131(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning and evolutionary analysis of msh-like homeobox genes from mouse, zebrafish and ascidian."
    Holland P.W.H.
    Gene 98:253-257(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-202.
  4. "Position-dependent expression of two related homeobox genes in developing vertebrate limbs."
    Davidson D.R., Crawley A., Hill R.E., Tickle C.
    Nature 352:429-431(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The RRM domain of MINT, a novel msx2 binding protein, recognizes and regulates the rat osteocalcin promoter."
    Newberry E.P., Latifi T., Towler D.A.
    Biochemistry 38:10678-10690(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MINT.
  6. "Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2."
    Kim Y.J., Lee M.H., Wozney J.M., Cho J.Y., Ryoo H.M.
    J. Biol. Chem. 279:50773-50780(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DNA-BINDING.

Entry informationi

Entry nameiMSX2_MOUSE
AccessioniPrimary (citable) accession number: Q03358
Secondary accession number(s): Q63856
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: February 4, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.