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Protein

Thrombospondin-2

Gene

Thbs2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • negative regulation of angiogenesis Source: UniProtKB
  • positive regulation of synapse assembly Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_280178. Signaling by PDGF.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-2
Gene namesi
Name:Thbs2
Synonyms:Tsp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98738. Thbs2.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • platelet alpha granule Source: MGI
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 11721154Thrombospondin-2PRO_0000035847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi266 – 266InterchainCurated
Disulfide bondi270 – 270InterchainCurated
Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi393 ↔ 425By similarity
Disulfide bondi397 ↔ 430By similarity
Disulfide bondi408 ↔ 415By similarity
Disulfide bondi449 ↔ 486By similarity
Disulfide bondi453 ↔ 491By similarity
Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi464 ↔ 476By similarity
Disulfide bondi506 ↔ 543By similarity
Disulfide bondi510 ↔ 548By similarity
Disulfide bondi521 ↔ 533By similarity
Disulfide bondi553 ↔ 564By similarity
Disulfide bondi558 ↔ 574By similarity
Disulfide bondi577 ↔ 588By similarity
Glycosylationi584 – 5841N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi594 ↔ 610By similarity
Disulfide bondi601 ↔ 619By similarity
Disulfide bondi622 ↔ 646By similarity
Disulfide bondi652 ↔ 665By similarity
Disulfide bondi659 ↔ 678By similarity
Disulfide bondi680 ↔ 691By similarity
Disulfide bondi707 ↔ 715By similarity
Glycosylationi710 – 7101N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi720 ↔ 740By similarity
Disulfide bondi756 ↔ 776By similarity
Disulfide bondi779 ↔ 799By similarity
Disulfide bondi815 ↔ 835By similarity
Disulfide bondi838 ↔ 858By similarity
Disulfide bondi876 ↔ 896By similarity
Disulfide bondi912 ↔ 932By similarity
Disulfide bondi948 ↔ 1169By similarity
Glycosylationi1069 – 10691N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ03350.
PaxDbiQ03350.
PRIDEiQ03350.

PTM databases

PhosphoSiteiQ03350.

Expressioni

Gene expression databases

BgeeiQ03350.
CleanExiMM_THBS2.
ExpressionAtlasiQ03350. baseline and differential.
GenevisibleiQ03350. MM.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked. Can bind to fibrinogen, fibronectin, laminin and type V collagen (By similarity). Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS2 with CD36. Can bind to fibrinogen, fibronectin, laminin.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Jag1Q637222EBI-4567830,EBI-4567800From a different organism.
LRP1Q079542EBI-4567830,EBI-1046087From a different organism.

Protein-protein interaction databases

BioGridi204176. 2 interactions.
IntActiQ03350. 3 interactions.
MINTiMINT-4115682.
STRINGi10090.ENSMUSP00000128308.

Structurei

3D structure databases

ProteinModelPortaliQ03350.
SMRiQ03350. Positions 27-226, 381-549, 551-1171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 215197Laminin G-likeAdd
BLAST
Domaini318 – 37558VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini381 – 43151TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini437 – 49256TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini494 – 54956TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini549 – 58941EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini648 – 69245EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Repeati693 – 72836TSP type-3 1Add
BLAST
Repeati729 – 76436TSP type-3 2Add
BLAST
Repeati765 – 78723TSP type-3 3Add
BLAST
Repeati788 – 82336TSP type-3 4Add
BLAST
Repeati824 – 84623TSP type-3 5Add
BLAST
Repeati847 – 88438TSP type-3 6Add
BLAST
Repeati885 – 92036TSP type-3 7Add
BLAST
Repeati921 – 95636TSP type-3 8Add
BLAST
Domaini960 – 1172213TSP C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 232214Heparin-bindingSequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi928 – 9303Cell attachment siteSequence Analysis

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 3 TSP type-1 domains.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00550000074507.
HOGENOMiHOG000007542.
HOVERGENiHBG018006.
InParanoidiQ03350.
KOiK04659.
OMAiTEKQVCE.
OrthoDBiEOG76QFGD.
TreeFamiTF324917.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR024731. EGF_dom_MSP1-like.
IPR001791. Laminin_G.
IPR015455. Thrombospondin-2.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR10199:SF10. PTHR10199:SF10. 1 hit.
PfamiPF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWALALLAL GIGPRASAGD HVKDTSFDLF SISNINRKTI GAKQFRGPDP
60 70 80 90 100
GVPAYRFVRF DYIPPVNTDD LNRIVKLARR KEGFFLTAQL KQDRKSRGTL
110 120 130 140 150
LVLEGPGTSQ RQFEIVSNGP GDTLDLNYWV EGNQHTNFLE DVGLADSQWK
160 170 180 190 200
NVTVQVASDT YSLYVGCDLI DSVTLEEPFY EQLEVDRSRM YVAKGASRES
210 220 230 240 250
HFRGLLQNVH LVFADSVEDI LSKKGCQHSQ GAEVNTISEH TETLHLSPHI
260 270 280 290 300
TTDLVVQGVE KAQEVCTHSC EELSNMMNEL SGLHVMVNQL SKNLERVSSD
310 320 330 340 350
NQFLLELIGG PLKTRNMSAC VQEGRIFAEN ETWVVDSCTT CTCKKFKTVC
360 370 380 390 400
HQITCSPATC ANPSFVEGEC CPSCSHSADS DEGWSPWAEW TECSVTCGSG
410 420 430 440 450
TQQRGRSCDV TSNTCLGPSI QTRTCSLGKC DTRIRQNGGW SHWSPWSSCS
460 470 480 490 500
VTCGVGNVTR IRLCNSPVPQ MGGKNCKGSG RETKPCQRDP CPIDGRWSPW
510 520 530 540 550
SPWSACTVTC AGGIRERSRV CNSPEPQYGG KDCVGDVTEH QMCNKRSCPI
560 570 580 590 600
DGCLSNPCFP GAKCNSFPDG SWSCGSCPVG FLGNGTHCED LDECAVVTDI
610 620 630 640 650
CFSTNKAPRC VNTNPGFHCL PCPPRYKGNQ PFGVGLEDAR TEKQVCEPEN
660 670 680 690 700
PCKDKTHSCH KNAECIYLGH FSDPMYKCEC QIGYAGDGLI CGEDSDLDGW
710 720 730 740 750
PNNNLVCATN ATYHCIKDNC PKLPNSGQED FDKDGIGDAC DEDDDNDGVS
760 770 780 790 800
DEKDNCQLLF NPRQLDYDKD EVGDRCDNCP YVHNPAQIDT DNNGEGDACS
810 820 830 840 850
VDIDGDDVFN ERDNCPYVYN TDQRDTDGDG VGDHCDNCPL MHNPDQIDQD
860 870 880 890 900
NDLVGDQCDN NEDIDDDGHQ NNQDNCPYIS NSNQADHDND GKGDACDSDD
910 920 930 940 950
DNDGVPDDRD NCRLVFNPDQ EDSDGDGRGD ICKDDFDNDN VPDIDDVCPE
960 970 980 990 1000
NNAITETDFR NFQMVPLDPK GTTQIDPNWV IRHQGKELVQ TANSDPGIAV
1010 1020 1030 1040 1050
GFDEFGSVDF SGTFYVNTDR DDDYAGFVFG YQSSSRFYVV MWKQVTQTYW
1060 1070 1080 1090 1100
EDKPSRAYGY SGVSLKVVNS TTGTGEHLRN ALWHTGNTEG QVRTLWHDPK
1110 1120 1130 1140 1150
NIGWKDYTAY RWHLIHRPKT GYMRVLVHEG KQVMADSGPI YDQTYAGGRL
1160 1170
GLFVFSQEMV YFSDLKYECR DA
Length:1,172
Mass (Da):129,882
Last modified:July 27, 2011 - v2
Checksum:i020ACD7EB5137B25
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti225 – 2251G → S in AAA53064 (PubMed:1371115).Curated
Sequence conflicti225 – 2251G → S in AAA40432 (PubMed:1712771).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07803 mRNA. Translation: AAA53064.1.
AF549256 Genomic DNA. Translation: AAO16244.1.
CH466630 Genomic DNA. Translation: EDL20481.1.
M64866 mRNA. Translation: AAA40432.1.
CCDSiCCDS49958.1.
PIRiA42587.
RefSeqiNP_035711.2. NM_011581.3.
UniGeneiMm.26688.

Genome annotation databases

EnsembliENSMUST00000170872; ENSMUSP00000128308; ENSMUSG00000023885.
GeneIDi21826.
KEGGimmu:21826.
UCSCiuc008anb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07803 mRNA. Translation: AAA53064.1.
AF549256 Genomic DNA. Translation: AAO16244.1.
CH466630 Genomic DNA. Translation: EDL20481.1.
M64866 mRNA. Translation: AAA40432.1.
CCDSiCCDS49958.1.
PIRiA42587.
RefSeqiNP_035711.2. NM_011581.3.
UniGeneiMm.26688.

3D structure databases

ProteinModelPortaliQ03350.
SMRiQ03350. Positions 27-226, 381-549, 551-1171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204176. 2 interactions.
IntActiQ03350. 3 interactions.
MINTiMINT-4115682.
STRINGi10090.ENSMUSP00000128308.

PTM databases

PhosphoSiteiQ03350.

Proteomic databases

MaxQBiQ03350.
PaxDbiQ03350.
PRIDEiQ03350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170872; ENSMUSP00000128308; ENSMUSG00000023885.
GeneIDi21826.
KEGGimmu:21826.
UCSCiuc008anb.2. mouse.

Organism-specific databases

CTDi7058.
MGIiMGI:98738. Thbs2.

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00550000074507.
HOGENOMiHOG000007542.
HOVERGENiHBG018006.
InParanoidiQ03350.
KOiK04659.
OMAiTEKQVCE.
OrthoDBiEOG76QFGD.
TreeFamiTF324917.

Enzyme and pathway databases

ReactomeiREACT_280178. Signaling by PDGF.

Miscellaneous databases

ChiTaRSiThbs2. mouse.
NextBioi301256.
PROiQ03350.
SOURCEiSearch...

Gene expression databases

BgeeiQ03350.
CleanExiMM_THBS2.
ExpressionAtlasiQ03350. baseline and differential.
GenevisibleiQ03350. MM.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR024731. EGF_dom_MSP1-like.
IPR001791. Laminin_G.
IPR015455. Thrombospondin-2.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR10199:SF10. PTHR10199:SF10. 1 hit.
PfamiPF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development."
    Laherty C.D., O'Rourke K., Wolf F.W., Katz R., Seldin M.F., Dixit V.M.
    J. Biol. Chem. 267:3274-3281(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic sequence analysis in the mouse t-complex region."
    Brathwaite M., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R.
    Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129S6/SvEvTac.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "A second, expressed thrombospondin gene (Thbs2) exists in the mouse genome."
    Bornstein P., O'Rourke K., Wikstrom K., Wolf F.W., Katz R., Li P., Dixit V.M.
    J. Biol. Chem. 266:12821-12824(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-873.
  5. "The antiangiogenic effect of thrombospondin-2 is mediated by CD36 and modulated by histidine-rich glycoprotein."
    Simantov R., Febbraio M., Silverstein R.L.
    Matrix Biol. 24:27-34(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CD36 AND HRG, FUNCTION.

Entry informationi

Entry nameiTSP2_MOUSE
AccessioniPrimary (citable) accession number: Q03350
Secondary accession number(s): Q8CG21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.