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Q03350

- TSP2_MOUSE

UniProt

Q03350 - TSP2_MOUSE

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Protein
Thrombospondin-2
Gene
Thbs2, Tsp2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.1 Publication

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. heparin binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. negative regulation of angiogenesis Source: UniProtKB
  3. positive regulation of synapse assembly Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-2
Gene namesi
Name:Thbs2
Synonyms:Tsp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:98738. Thbs2.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: MGI
  2. extracellular matrix Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed prediction
Add
BLAST
Chaini19 – 11721154Thrombospondin-2
PRO_0000035847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi151 – 1511N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi266 – 266Interchain Inferred
Disulfide bondi270 – 270Interchain Inferred
Glycosylationi316 – 3161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi330 – 3301N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi393 ↔ 425 By similarity
Disulfide bondi397 ↔ 430 By similarity
Disulfide bondi408 ↔ 415 By similarity
Disulfide bondi449 ↔ 486 By similarity
Disulfide bondi453 ↔ 491 By similarity
Glycosylationi457 – 4571N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi464 ↔ 476 By similarity
Disulfide bondi506 ↔ 543 By similarity
Disulfide bondi510 ↔ 548 By similarity
Disulfide bondi521 ↔ 533 By similarity
Disulfide bondi553 ↔ 564 By similarity
Disulfide bondi558 ↔ 574 By similarity
Disulfide bondi577 ↔ 588 By similarity
Glycosylationi584 – 5841N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi594 ↔ 610 By similarity
Disulfide bondi601 ↔ 619 By similarity
Disulfide bondi622 ↔ 646 By similarity
Disulfide bondi652 ↔ 665 By similarity
Disulfide bondi659 ↔ 678 By similarity
Disulfide bondi680 ↔ 691 By similarity
Disulfide bondi707 ↔ 715 By similarity
Glycosylationi710 – 7101N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi720 ↔ 740 By similarity
Disulfide bondi756 ↔ 776 By similarity
Disulfide bondi779 ↔ 799 By similarity
Disulfide bondi815 ↔ 835 By similarity
Disulfide bondi838 ↔ 858 By similarity
Disulfide bondi876 ↔ 896 By similarity
Disulfide bondi912 ↔ 932 By similarity
Disulfide bondi948 ↔ 1169 By similarity
Glycosylationi1069 – 10691N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ03350.
PRIDEiQ03350.

PTM databases

PhosphoSiteiQ03350.

Expressioni

Gene expression databases

ArrayExpressiQ03350.
BgeeiQ03350.
CleanExiMM_THBS2.
GenevestigatoriQ03350.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked. Can bind to fibrinogen, fibronectin, laminin and type V collagen By similarity. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS2 with CD36. Can bind to fibrinogen, fibronectin, laminin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Jag1Q637222EBI-4567830,EBI-4567800From a different organism.
LRP1Q079542EBI-4567830,EBI-1046087From a different organism.

Protein-protein interaction databases

BioGridi204176. 2 interactions.
IntActiQ03350. 3 interactions.
MINTiMINT-4115682.

Structurei

3D structure databases

ProteinModelPortaliQ03350.
SMRiQ03350. Positions 27-226, 381-549, 551-1171.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 215197Laminin G-like
Add
BLAST
Domaini318 – 37558VWFC
Add
BLAST
Domaini381 – 43151TSP type-1 1
Add
BLAST
Domaini437 – 49256TSP type-1 2
Add
BLAST
Domaini494 – 54956TSP type-1 3
Add
BLAST
Domaini549 – 58941EGF-like 1
Add
BLAST
Domaini648 – 69245EGF-like 2
Add
BLAST
Repeati693 – 72836TSP type-3 1
Add
BLAST
Repeati729 – 76436TSP type-3 2
Add
BLAST
Repeati765 – 78723TSP type-3 3
Add
BLAST
Repeati788 – 82336TSP type-3 4
Add
BLAST
Repeati824 – 84623TSP type-3 5
Add
BLAST
Repeati847 – 88438TSP type-3 6
Add
BLAST
Repeati885 – 92036TSP type-3 7
Add
BLAST
Repeati921 – 95636TSP type-3 8
Add
BLAST
Domaini960 – 1172213TSP C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 232214Heparin-binding Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi928 – 9303Cell attachment site Reviewed prediction

Sequence similaritiesi

Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00550000074507.
HOGENOMiHOG000007542.
HOVERGENiHBG018006.
InParanoidiQ8CG21.
KOiK04659.
OMAiTEKQVCE.
OrthoDBiEOG76QFGD.
TreeFamiTF324917.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR024731. EGF_dom_MSP1-like.
IPR001791. Laminin_G.
IPR015455. Thrombospondin-2.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR10199:SF10. PTHR10199:SF10. 1 hit.
PfamiPF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03350-1 [UniParc]FASTAAdd to Basket

« Hide

MLWALALLAL GIGPRASAGD HVKDTSFDLF SISNINRKTI GAKQFRGPDP     50
GVPAYRFVRF DYIPPVNTDD LNRIVKLARR KEGFFLTAQL KQDRKSRGTL 100
LVLEGPGTSQ RQFEIVSNGP GDTLDLNYWV EGNQHTNFLE DVGLADSQWK 150
NVTVQVASDT YSLYVGCDLI DSVTLEEPFY EQLEVDRSRM YVAKGASRES 200
HFRGLLQNVH LVFADSVEDI LSKKGCQHSQ GAEVNTISEH TETLHLSPHI 250
TTDLVVQGVE KAQEVCTHSC EELSNMMNEL SGLHVMVNQL SKNLERVSSD 300
NQFLLELIGG PLKTRNMSAC VQEGRIFAEN ETWVVDSCTT CTCKKFKTVC 350
HQITCSPATC ANPSFVEGEC CPSCSHSADS DEGWSPWAEW TECSVTCGSG 400
TQQRGRSCDV TSNTCLGPSI QTRTCSLGKC DTRIRQNGGW SHWSPWSSCS 450
VTCGVGNVTR IRLCNSPVPQ MGGKNCKGSG RETKPCQRDP CPIDGRWSPW 500
SPWSACTVTC AGGIRERSRV CNSPEPQYGG KDCVGDVTEH QMCNKRSCPI 550
DGCLSNPCFP GAKCNSFPDG SWSCGSCPVG FLGNGTHCED LDECAVVTDI 600
CFSTNKAPRC VNTNPGFHCL PCPPRYKGNQ PFGVGLEDAR TEKQVCEPEN 650
PCKDKTHSCH KNAECIYLGH FSDPMYKCEC QIGYAGDGLI CGEDSDLDGW 700
PNNNLVCATN ATYHCIKDNC PKLPNSGQED FDKDGIGDAC DEDDDNDGVS 750
DEKDNCQLLF NPRQLDYDKD EVGDRCDNCP YVHNPAQIDT DNNGEGDACS 800
VDIDGDDVFN ERDNCPYVYN TDQRDTDGDG VGDHCDNCPL MHNPDQIDQD 850
NDLVGDQCDN NEDIDDDGHQ NNQDNCPYIS NSNQADHDND GKGDACDSDD 900
DNDGVPDDRD NCRLVFNPDQ EDSDGDGRGD ICKDDFDNDN VPDIDDVCPE 950
NNAITETDFR NFQMVPLDPK GTTQIDPNWV IRHQGKELVQ TANSDPGIAV 1000
GFDEFGSVDF SGTFYVNTDR DDDYAGFVFG YQSSSRFYVV MWKQVTQTYW 1050
EDKPSRAYGY SGVSLKVVNS TTGTGEHLRN ALWHTGNTEG QVRTLWHDPK 1100
NIGWKDYTAY RWHLIHRPKT GYMRVLVHEG KQVMADSGPI YDQTYAGGRL 1150
GLFVFSQEMV YFSDLKYECR DA 1172
Length:1,172
Mass (Da):129,882
Last modified:July 27, 2011 - v2
Checksum:i020ACD7EB5137B25
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti225 – 2251G → S in AAA53064. 1 Publication
Sequence conflicti225 – 2251G → S in AAA40432. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07803 mRNA. Translation: AAA53064.1.
AF549256 Genomic DNA. Translation: AAO16244.1.
CH466630 Genomic DNA. Translation: EDL20481.1.
M64866 mRNA. Translation: AAA40432.1.
CCDSiCCDS49958.1.
PIRiA42587.
RefSeqiNP_035711.2. NM_011581.3.
UniGeneiMm.26688.

Genome annotation databases

EnsembliENSMUST00000170872; ENSMUSP00000128308; ENSMUSG00000023885.
GeneIDi21826.
KEGGimmu:21826.
UCSCiuc008anb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07803 mRNA. Translation: AAA53064.1 .
AF549256 Genomic DNA. Translation: AAO16244.1 .
CH466630 Genomic DNA. Translation: EDL20481.1 .
M64866 mRNA. Translation: AAA40432.1 .
CCDSi CCDS49958.1.
PIRi A42587.
RefSeqi NP_035711.2. NM_011581.3.
UniGenei Mm.26688.

3D structure databases

ProteinModelPortali Q03350.
SMRi Q03350. Positions 27-226, 381-549, 551-1171.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204176. 2 interactions.
IntActi Q03350. 3 interactions.
MINTi MINT-4115682.

PTM databases

PhosphoSitei Q03350.

Proteomic databases

PaxDbi Q03350.
PRIDEi Q03350.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000170872 ; ENSMUSP00000128308 ; ENSMUSG00000023885 .
GeneIDi 21826.
KEGGi mmu:21826.
UCSCi uc008anb.2. mouse.

Organism-specific databases

CTDi 7058.
MGIi MGI:98738. Thbs2.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00550000074507.
HOGENOMi HOG000007542.
HOVERGENi HBG018006.
InParanoidi Q8CG21.
KOi K04659.
OMAi TEKQVCE.
OrthoDBi EOG76QFGD.
TreeFami TF324917.

Miscellaneous databases

ChiTaRSi THBS2. mouse.
NextBioi 301256.
PROi Q03350.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q03350.
Bgeei Q03350.
CleanExi MM_THBS2.
Genevestigatori Q03350.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR024731. EGF_dom_MSP1-like.
IPR001791. Laminin_G.
IPR015455. Thrombospondin-2.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view ]
PANTHERi PTHR10199:SF10. PTHR10199:SF10. 1 hit.
Pfami PF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development."
    Laherty C.D., O'Rourke K., Wolf F.W., Katz R., Seldin M.F., Dixit V.M.
    J. Biol. Chem. 267:3274-3281(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic sequence analysis in the mouse t-complex region."
    Brathwaite M., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R.
    Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129S6/SvEvTac.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "A second, expressed thrombospondin gene (Thbs2) exists in the mouse genome."
    Bornstein P., O'Rourke K., Wikstrom K., Wolf F.W., Katz R., Li P., Dixit V.M.
    J. Biol. Chem. 266:12821-12824(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-873.
  5. "The antiangiogenic effect of thrombospondin-2 is mediated by CD36 and modulated by histidine-rich glycoprotein."
    Simantov R., Febbraio M., Silverstein R.L.
    Matrix Biol. 24:27-34(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CD36 AND HRG, FUNCTION.

Entry informationi

Entry nameiTSP2_MOUSE
AccessioniPrimary (citable) accession number: Q03350
Secondary accession number(s): Q8CG21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi