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Q03350

- TSP2_MOUSE

UniProt

Q03350 - TSP2_MOUSE

Protein

Thrombospondin-2

Gene

Thbs2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.1 Publication

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. heparin binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. negative regulation of angiogenesis Source: UniProtKB
    3. positive regulation of synapse assembly Source: Ensembl

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thrombospondin-2
    Gene namesi
    Name:Thbs2
    Synonyms:Tsp2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:98738. Thbs2.

    Subcellular locationi

    GO - Cellular componenti

    1. basement membrane Source: MGI
    2. extracellular matrix Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 11721154Thrombospondin-2PRO_0000035847Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi266 – 266InterchainCurated
    Disulfide bondi270 – 270InterchainCurated
    Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi393 ↔ 425By similarity
    Disulfide bondi397 ↔ 430By similarity
    Disulfide bondi408 ↔ 415By similarity
    Disulfide bondi449 ↔ 486By similarity
    Disulfide bondi453 ↔ 491By similarity
    Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi464 ↔ 476By similarity
    Disulfide bondi506 ↔ 543By similarity
    Disulfide bondi510 ↔ 548By similarity
    Disulfide bondi521 ↔ 533By similarity
    Disulfide bondi553 ↔ 564By similarity
    Disulfide bondi558 ↔ 574By similarity
    Disulfide bondi577 ↔ 588By similarity
    Glycosylationi584 – 5841N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi594 ↔ 610By similarity
    Disulfide bondi601 ↔ 619By similarity
    Disulfide bondi622 ↔ 646By similarity
    Disulfide bondi652 ↔ 665By similarity
    Disulfide bondi659 ↔ 678By similarity
    Disulfide bondi680 ↔ 691By similarity
    Disulfide bondi707 ↔ 715By similarity
    Glycosylationi710 – 7101N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi720 ↔ 740By similarity
    Disulfide bondi756 ↔ 776By similarity
    Disulfide bondi779 ↔ 799By similarity
    Disulfide bondi815 ↔ 835By similarity
    Disulfide bondi838 ↔ 858By similarity
    Disulfide bondi876 ↔ 896By similarity
    Disulfide bondi912 ↔ 932By similarity
    Disulfide bondi948 ↔ 1169By similarity
    Glycosylationi1069 – 10691N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ03350.
    PRIDEiQ03350.

    PTM databases

    PhosphoSiteiQ03350.

    Expressioni

    Gene expression databases

    ArrayExpressiQ03350.
    BgeeiQ03350.
    CleanExiMM_THBS2.
    GenevestigatoriQ03350.

    Interactioni

    Subunit structurei

    Homotrimer; disulfide-linked. Can bind to fibrinogen, fibronectin, laminin and type V collagen By similarity. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS2 with CD36. Can bind to fibrinogen, fibronectin, laminin.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Jag1Q637222EBI-4567830,EBI-4567800From a different organism.
    LRP1Q079542EBI-4567830,EBI-1046087From a different organism.

    Protein-protein interaction databases

    BioGridi204176. 2 interactions.
    IntActiQ03350. 3 interactions.
    MINTiMINT-4115682.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03350.
    SMRiQ03350. Positions 27-226, 381-549, 551-1171.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 215197Laminin G-likeAdd
    BLAST
    Domaini318 – 37558VWFCPROSITE-ProRule annotationAdd
    BLAST
    Domaini381 – 43151TSP type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini437 – 49256TSP type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini494 – 54956TSP type-1 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini549 – 58941EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini648 – 69245EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati693 – 72836TSP type-3 1Add
    BLAST
    Repeati729 – 76436TSP type-3 2Add
    BLAST
    Repeati765 – 78723TSP type-3 3Add
    BLAST
    Repeati788 – 82336TSP type-3 4Add
    BLAST
    Repeati824 – 84623TSP type-3 5Add
    BLAST
    Repeati847 – 88438TSP type-3 6Add
    BLAST
    Repeati885 – 92036TSP type-3 7Add
    BLAST
    Repeati921 – 95636TSP type-3 8Add
    BLAST
    Domaini960 – 1172213TSP C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni19 – 232214Heparin-bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi928 – 9303Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Belongs to the thrombospondin family.Curated
    Contains 2 EGF-like domains.PROSITE-ProRule annotation
    Contains 1 laminin G-like domain.Curated
    Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
    Contains 3 TSP type-1 domains.PROSITE-ProRule annotation
    Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation
    Contains 1 VWFC domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00550000074507.
    HOGENOMiHOG000007542.
    HOVERGENiHBG018006.
    InParanoidiQ8CG21.
    KOiK04659.
    OMAiTEKQVCE.
    OrthoDBiEOG76QFGD.
    TreeFamiTF324917.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    4.10.1080.10. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR024731. EGF_dom_MSP1-like.
    IPR001791. Laminin_G.
    IPR015455. Thrombospondin-2.
    IPR000884. Thrombospondin_1_rpt.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR017897. Thrombospondin_3_rpt.
    IPR008859. Thrombospondin_C.
    IPR028974. TSP_type-3_rpt.
    IPR001007. VWF_C.
    [Graphical view]
    PANTHERiPTHR10199:SF10. PTHR10199:SF10. 1 hit.
    PfamiPF12947. EGF_3. 1 hit.
    PF07645. EGF_CA. 1 hit.
    PF00090. TSP_1. 3 hits.
    PF02412. TSP_3. 7 hits.
    PF05735. TSP_C. 1 hit.
    PF00093. VWC. 1 hit.
    [Graphical view]
    SMARTiSM00181. EGF. 3 hits.
    SM00209. TSP1. 3 hits.
    SM00210. TSPN. 1 hit.
    SM00214. VWC. 1 hit.
    [Graphical view]
    SUPFAMiSSF103647. SSF103647. 3 hits.
    SSF49899. SSF49899. 2 hits.
    SSF82895. SSF82895. 3 hits.
    PROSITEiPS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS50092. TSP1. 3 hits.
    PS51234. TSP3. 8 hits.
    PS51236. TSP_CTER. 1 hit.
    PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03350-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLWALALLAL GIGPRASAGD HVKDTSFDLF SISNINRKTI GAKQFRGPDP     50
    GVPAYRFVRF DYIPPVNTDD LNRIVKLARR KEGFFLTAQL KQDRKSRGTL 100
    LVLEGPGTSQ RQFEIVSNGP GDTLDLNYWV EGNQHTNFLE DVGLADSQWK 150
    NVTVQVASDT YSLYVGCDLI DSVTLEEPFY EQLEVDRSRM YVAKGASRES 200
    HFRGLLQNVH LVFADSVEDI LSKKGCQHSQ GAEVNTISEH TETLHLSPHI 250
    TTDLVVQGVE KAQEVCTHSC EELSNMMNEL SGLHVMVNQL SKNLERVSSD 300
    NQFLLELIGG PLKTRNMSAC VQEGRIFAEN ETWVVDSCTT CTCKKFKTVC 350
    HQITCSPATC ANPSFVEGEC CPSCSHSADS DEGWSPWAEW TECSVTCGSG 400
    TQQRGRSCDV TSNTCLGPSI QTRTCSLGKC DTRIRQNGGW SHWSPWSSCS 450
    VTCGVGNVTR IRLCNSPVPQ MGGKNCKGSG RETKPCQRDP CPIDGRWSPW 500
    SPWSACTVTC AGGIRERSRV CNSPEPQYGG KDCVGDVTEH QMCNKRSCPI 550
    DGCLSNPCFP GAKCNSFPDG SWSCGSCPVG FLGNGTHCED LDECAVVTDI 600
    CFSTNKAPRC VNTNPGFHCL PCPPRYKGNQ PFGVGLEDAR TEKQVCEPEN 650
    PCKDKTHSCH KNAECIYLGH FSDPMYKCEC QIGYAGDGLI CGEDSDLDGW 700
    PNNNLVCATN ATYHCIKDNC PKLPNSGQED FDKDGIGDAC DEDDDNDGVS 750
    DEKDNCQLLF NPRQLDYDKD EVGDRCDNCP YVHNPAQIDT DNNGEGDACS 800
    VDIDGDDVFN ERDNCPYVYN TDQRDTDGDG VGDHCDNCPL MHNPDQIDQD 850
    NDLVGDQCDN NEDIDDDGHQ NNQDNCPYIS NSNQADHDND GKGDACDSDD 900
    DNDGVPDDRD NCRLVFNPDQ EDSDGDGRGD ICKDDFDNDN VPDIDDVCPE 950
    NNAITETDFR NFQMVPLDPK GTTQIDPNWV IRHQGKELVQ TANSDPGIAV 1000
    GFDEFGSVDF SGTFYVNTDR DDDYAGFVFG YQSSSRFYVV MWKQVTQTYW 1050
    EDKPSRAYGY SGVSLKVVNS TTGTGEHLRN ALWHTGNTEG QVRTLWHDPK 1100
    NIGWKDYTAY RWHLIHRPKT GYMRVLVHEG KQVMADSGPI YDQTYAGGRL 1150
    GLFVFSQEMV YFSDLKYECR DA 1172
    Length:1,172
    Mass (Da):129,882
    Last modified:July 27, 2011 - v2
    Checksum:i020ACD7EB5137B25
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti225 – 2251G → S in AAA53064. (PubMed:1371115)Curated
    Sequence conflicti225 – 2251G → S in AAA40432. (PubMed:1712771)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07803 mRNA. Translation: AAA53064.1.
    AF549256 Genomic DNA. Translation: AAO16244.1.
    CH466630 Genomic DNA. Translation: EDL20481.1.
    M64866 mRNA. Translation: AAA40432.1.
    CCDSiCCDS49958.1.
    PIRiA42587.
    RefSeqiNP_035711.2. NM_011581.3.
    UniGeneiMm.26688.

    Genome annotation databases

    EnsembliENSMUST00000170872; ENSMUSP00000128308; ENSMUSG00000023885.
    GeneIDi21826.
    KEGGimmu:21826.
    UCSCiuc008anb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07803 mRNA. Translation: AAA53064.1 .
    AF549256 Genomic DNA. Translation: AAO16244.1 .
    CH466630 Genomic DNA. Translation: EDL20481.1 .
    M64866 mRNA. Translation: AAA40432.1 .
    CCDSi CCDS49958.1.
    PIRi A42587.
    RefSeqi NP_035711.2. NM_011581.3.
    UniGenei Mm.26688.

    3D structure databases

    ProteinModelPortali Q03350.
    SMRi Q03350. Positions 27-226, 381-549, 551-1171.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204176. 2 interactions.
    IntActi Q03350. 3 interactions.
    MINTi MINT-4115682.

    PTM databases

    PhosphoSitei Q03350.

    Proteomic databases

    PaxDbi Q03350.
    PRIDEi Q03350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000170872 ; ENSMUSP00000128308 ; ENSMUSG00000023885 .
    GeneIDi 21826.
    KEGGi mmu:21826.
    UCSCi uc008anb.2. mouse.

    Organism-specific databases

    CTDi 7058.
    MGIi MGI:98738. Thbs2.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00550000074507.
    HOGENOMi HOG000007542.
    HOVERGENi HBG018006.
    InParanoidi Q8CG21.
    KOi K04659.
    OMAi TEKQVCE.
    OrthoDBi EOG76QFGD.
    TreeFami TF324917.

    Miscellaneous databases

    ChiTaRSi THBS2. mouse.
    NextBioi 301256.
    PROi Q03350.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q03350.
    Bgeei Q03350.
    CleanExi MM_THBS2.
    Genevestigatori Q03350.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    4.10.1080.10. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR024731. EGF_dom_MSP1-like.
    IPR001791. Laminin_G.
    IPR015455. Thrombospondin-2.
    IPR000884. Thrombospondin_1_rpt.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR017897. Thrombospondin_3_rpt.
    IPR008859. Thrombospondin_C.
    IPR028974. TSP_type-3_rpt.
    IPR001007. VWF_C.
    [Graphical view ]
    PANTHERi PTHR10199:SF10. PTHR10199:SF10. 1 hit.
    Pfami PF12947. EGF_3. 1 hit.
    PF07645. EGF_CA. 1 hit.
    PF00090. TSP_1. 3 hits.
    PF02412. TSP_3. 7 hits.
    PF05735. TSP_C. 1 hit.
    PF00093. VWC. 1 hit.
    [Graphical view ]
    SMARTi SM00181. EGF. 3 hits.
    SM00209. TSP1. 3 hits.
    SM00210. TSPN. 1 hit.
    SM00214. VWC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103647. SSF103647. 3 hits.
    SSF49899. SSF49899. 2 hits.
    SSF82895. SSF82895. 3 hits.
    PROSITEi PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS50092. TSP1. 3 hits.
    PS51234. TSP3. 8 hits.
    PS51236. TSP_CTER. 1 hit.
    PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development."
      Laherty C.D., O'Rourke K., Wolf F.W., Katz R., Seldin M.F., Dixit V.M.
      J. Biol. Chem. 267:3274-3281(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genomic sequence analysis in the mouse t-complex region."
      Brathwaite M., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R.
      Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129S6/SvEvTac.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "A second, expressed thrombospondin gene (Thbs2) exists in the mouse genome."
      Bornstein P., O'Rourke K., Wikstrom K., Wolf F.W., Katz R., Li P., Dixit V.M.
      J. Biol. Chem. 266:12821-12824(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-873.
    5. "The antiangiogenic effect of thrombospondin-2 is mediated by CD36 and modulated by histidine-rich glycoprotein."
      Simantov R., Febbraio M., Silverstein R.L.
      Matrix Biol. 24:27-34(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CD36 AND HRG, FUNCTION.

    Entry informationi

    Entry nameiTSP2_MOUSE
    AccessioniPrimary (citable) accession number: Q03350
    Secondary accession number(s): Q8CG21
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3